丹参与其伪品、混淆品比较鉴别分析
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摘要
目的对正品丹参及其伪品(牛蒡根、川续断)、混淆品(甘肃丹参、滇丹参及白花丹参)进行鉴别研究。方法采用性状鉴别、显微鉴别、薄层色谱(TLC)及高效液相色谱(HPLC)法对丹参及其伪品、混淆品进行鉴别。结果性状鉴别研究表明,从外观形状、颜色等能较好区分丹参与牛蒡根、川续断、甘肃丹参、滇丹参,但是不易区分白花丹参;显微鉴别研究表明,丹参与牛蒡根、川续断、白花丹参可通过显微粉末鉴别区分,而丹参与甘肃丹参、滇丹参显微差别小,较难区分;薄层色谱法可以区别伪品牛蒡根、川续断及混淆品甘肃丹参、滇丹参,难以鉴别白花丹参;HPLC特征图谱、含量测定与薄层色谱三者结果一致,可以区别丹参与甘肃丹参、滇丹参,但无法区别白花丹参。结论综合运用本研究的鉴别方法可以有效鉴别正品丹参及其伪品、混淆品。
Objective To identify the Salviae miltiorrhizae Radix et Rhizoma and its counterfeit(roots of Arctium lappa and Dipsacus asper) and adulterants(roots of Salvia przewalskii,Salvia yunnanensis and Salvia miltiorrhiza Bge. f. alba). Methods The identification of Salviae miltiorrhizae Radix et Rhizoma and its counterfeit and adulterants were based on the methods of trait characteristics,microscope,Thin layer chromatography(TLC) and high-performance liquid chromatography(HPLC). Results The study of trait characteristics indicated that Salviae miltiorrhizae Radix et Rhizoma and roots of Arctium lappa,Dipsacus asper,Salvia przewalskii,and Salvia yunnanensis could be distinguished from the appearance shape,color and so on,but it was difficult to distinguish the roots of Salvia miltiorrhiza Bge. f. alba. Microscopic identification results showed that the microscopic characteristics of Salviae miltiorrhizae Radix et Rhizoma and roots of Arctium lappa,Dipsacus asper were obviously different,while roots of Salvia miltiorrhiza and Salvia przewalskii,Salvia yunnanensis was difficult to distinguish. TLC could distinguish the roots of Arctium lappa,Dipsacus asper,Salvia przewalskii,and Salvia yunnanensis,but it was difficult to identify the roots of Salvia miltiorrhiza Bge. f. alba. The HPLC characteristic spectra and the content determination were consistent with the results of TLC,which showed that there was a certain difference between Salviae miltiorrhizae Radix et Rhizoma and the roots of Salvia przewalskii,Salvia yunnanensis,but it was difficult to distinguish the roots of Salvia miltiorrhiza Bge. f. alba. Conclusion The methods of this study can effectively distinguish Salviae miltiorrhizae Radix et Rhizoma and its counterfeit and adulterants.
Objective To identify the Salviae miltiorrhizae Radix et Rhizoma and its counterfeit(roots of Arctium lappa and Dipsacus asper) and adulterants(roots of Salvia przewalskii,Salvia yunnanensis and Salvia miltiorrhiza Bge. f. alba). Methods The identification of Salviae miltiorrhizae Radix et Rhizoma and its counterfeit and adulterants were based on the methods of trait characteristics,microscope,Thin layer chromatography(TLC) and high-performance liquid chromatography(HPLC). Results The study of trait characteristics indicated that Salviae miltiorrhizae Radix et Rhizoma and roots of Arctium lappa,Dipsacus asper,Salvia przewalskii,and Salvia yunnanensis could be distinguished from the appearance shape,color and so on,but it was difficult to distinguish the roots of Salvia miltiorrhiza Bge. f. alba. Microscopic identification results showed that the microscopic characteristics of Salviae miltiorrhizae Radix et Rhizoma and roots of Arctium lappa,Dipsacus asper were obviously different,while roots of Salvia miltiorrhiza and Salvia przewalskii,Salvia yunnanensis was difficult to distinguish. TLC could distinguish the roots of Arctium lappa,Dipsacus asper,Salvia przewalskii,and Salvia yunnanensis,but it was difficult to identify the roots of Salvia miltiorrhiza Bge. f. alba. The HPLC characteristic spectra and the content determination were consistent with the results of TLC,which showed that there was a certain difference between Salviae miltiorrhizae Radix et Rhizoma and the roots of Salvia przewalskii,Salvia yunnanensis,but it was difficult to distinguish the roots of Salvia miltiorrhiza Bge. f. alba. Conclusion The methods of this study can effectively distinguish Salviae miltiorrhizae Radix et Rhizoma and its counterfeit and adulterants.
引文
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[12] 李海鹰,邵林,岳林峰.丹参及其伪品牛蒡根的理化鉴别[J].中药材,1999,22(9):449-451.
[13] 赵一烈,王心伟,王大猷,等.白花丹参的生药鉴定简报[J].中药材科技,1982(3):35-37.
[14] 杨先国,刘塔斯,林丽美,等.湖南地区白花丹参的引种试验研究[J].中国实验方剂学杂志,2012,18(1):117-120.
[2] 高兵.丹参的药理作用及临床应用分析[J].中国现代药物应用,2018,12(1):196-197.
[3] 郭兰萍,黄璐琦,华国栋,等.丹参地理变异及其道地性探讨[J].现代中药研究与实践,2006,20(5):3-6.
[4] 王涵,杨娜,谭静,等.丹参化学成分、药理作用及临床应用的研究进展[J].特产研究,2018,40(1):48-53.
[5] 苟占平,吕应年,龚先玲,等.丹参类中药的品种与鉴定研究概述[J].时珍国医国药,2008,19(12):2869-2871.
[6] 陈慧.丹参市售混淆品和伪品鉴别[J].广东微量元素科学,2016,23(4):69-71.
[7] 于广孝.丹参及其常见伪品鉴别[J].中国继续医学教育,2015,7(21):204-205.
[8] 张军伟,白明.丹参及其伪品的鉴别[J].河南中医学院学报,2004,19(11):32.
[9] 梁素娇,刘如良.丹参及其易混淆品的真伪分析[J].光明中医,2014,29(10):2215-2238.
[10] 马逾英,卢晓琳,翟萌,杨莹.几种中药材及其混伪品的微性状对比鉴别[J].中国中药杂志,2012,37(8):1161-1164.
[11] 季峰.丹参与伪品续断的区别[J].国医论坛,2006,21(3):52.
[12] 李海鹰,邵林,岳林峰.丹参及其伪品牛蒡根的理化鉴别[J].中药材,1999,22(9):449-451.
[13] 赵一烈,王心伟,王大猷,等.白花丹参的生药鉴定简报[J].中药材科技,1982(3):35-37.
[14] 杨先国,刘塔斯,林丽美,等.湖南地区白花丹参的引种试验研究[J].中国实验方剂学杂志,2012,18(1):117-120.
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