基于无患子科ITS序列探讨龙荔的系统发育
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摘要
【目的】利用无患子科间隔转录区序列(ITS)探讨了龙荔的系统发育地位。【方法】通过无患子科27个属62个种ITS序列信息序列比对和系统发育分析,探讨龙荔和龙眼、荔枝之间的系统进化关系。【结果】基于ITS序列信息可以清楚区分无患子科内不同的种属,其属间ITS序列相似度为(91.0±4.4)%、种间为(96.3±2.8)%。龙荔与龙眼、荔枝之间的ITS序列同源性均为92%,低于龙眼与荔枝间的同源性(93%),但高于和红毛丹的同源性(91%),其ITS序列中有4.5%的碱基位点与龙眼和荔枝均不同,主要表现为T和C互换、A和G互换,共占序列差异位点的56.5%。以韶子属红毛丹为外类群的系统发育树显示,龙荔、荔枝、龙眼之间的进化发育明显,龙荔最早自成分支,其次是荔枝,最后是龙眼。【结论】ITS序列信息分析结果支持龙荔作为独立属的分类可能。
【Objective】To establish the phylogeny of longli, Dimocarpus confinis, based on its nuclear encoded internal transcribed spacer(ITS) sequences. 【Method】 The ITS sequences of 27 genera and 62 species of Sapindaceae were collected to examine the relationship among longli, longan(Dimocarpus longan) and litchi(Litchi chinensis).【Result】The genera in Sapindaceae family could be clearly classified by ITS sequence. The homology among the genera was(91.0±4.4)%, and among the species(96.3±2.8)%. The ITS sequence homology between longli and longan or litchi was 92%, which was lower than that between longan and litchi at 93% but higher than that between longli and rambutan(Nephelium lappaceum L.) at 91%. Four and half percent of the longli ITS sequence differed from those of longan and litchi. The differences were mainly changing between T and C or A and G that accounted for 56.5% of the total. Based on the phylogenetic tree of rambutan as the outgroup, the evolution of longli was shown to be the earliest to branch out, followed by litchi and longan. 【Conclusion】The result suggested classifying longli as a separate genus in the family.
【Objective】To establish the phylogeny of longli, Dimocarpus confinis, based on its nuclear encoded internal transcribed spacer(ITS) sequences. 【Method】 The ITS sequences of 27 genera and 62 species of Sapindaceae were collected to examine the relationship among longli, longan(Dimocarpus longan) and litchi(Litchi chinensis).【Result】The genera in Sapindaceae family could be clearly classified by ITS sequence. The homology among the genera was(91.0±4.4)%, and among the species(96.3±2.8)%. The ITS sequence homology between longli and longan or litchi was 92%, which was lower than that between longan and litchi at 93% but higher than that between longli and rambutan(Nephelium lappaceum L.) at 91%. Four and half percent of the longli ITS sequence differed from those of longan and litchi. The differences were mainly changing between T and C or A and G that accounted for 56.5% of the total. Based on the phylogenetic tree of rambutan as the outgroup, the evolution of longli was shown to be the earliest to branch out, followed by litchi and longan. 【Conclusion】The result suggested classifying longli as a separate genus in the family.
引文
[1]邱武陵, 章恢志.中国果树志·龙眼批把卷[M].北京:中国林业出版社,1996.QIU W L, ZHANG H Z.China Fruit Graphy:Longan and Loquat Tomus[M].Beijing:China Forestry Publishing House,1996.(in Chinese)
[2]潘丽梅, 朱建华, 刘冰浩, 等.广西龙虎山自然保护区龙荔种群生命表分析[J].园艺学报, 2011, 38(7):1349-1355.PAN L M, ZHU J H, LIU B H, et al.Life Table Analysis of Dimocarpus confinis Population in Guangxi Longhushan Nature Reserve[J].Acta Horticulturae Sinica,2011, 38(7):1349-1355.(in Chinese)
[3]陈秀萍, 蒋际谋, 许家辉, 等.不同砧木对龙眼生长的影响试验[J].中国南方果树, 2006, 35(5):21-22.CHE X P, JIANG J M, XU J H, et al.Reseach of longan development based on different storcks[J].South China Fruits, 2006, 35(5):21-22.(in Chinese)
[4]陆贵锋, 朱松生, 黄川, 等.龙荔嫁接龙眼、荔枝的试验初报[J].中国南方果树, 2016, 45(5):148-149. LU G F, ZHU S S, HUANG C, et al.research of grafting between Longli and Longan and Litchi[J].South China Fruits, 2016, 45(5):148-149.(in Chinese)
[5]刘冰浩,徐宁,朱建华, 等.广西龙眼种质耐寒性的CTR值鉴定[J].西南农业学报, 2006, 19(4):668-671.LIU B H, XU N, ZHU J H, et al.The CTR identification of chilling-tolerance of Longan germplasm in Guangxi[J].Southwest China Journalof Agricultural Science, 2006, 19(4):668-671.(in Chinese)
[6]姜帆,高慧颖,陈秀萍, 等.龙眼属rDNA的ITS序列分析[J].果树学报, 2008, 25(2): 262- 268.JIANG F, GAO H Y, CHE X P, et al.Analysis of ITS sequences of rDNA in Dimocarpus plants[J].Journalof Fruit Science, 2008, 25(2): 262- 268.(in Chinese)
[7]高慧颖,姜帆,陈秀萍, 等.不同地域的代表性基因型龙眼RAPD分析[J].福建林业科技, 2007,34(1): 67-71,88.GAO H Y, JIANG F, CHE X P, et al.Random Amplification Polymorphic DNA (RAPD) Analysis of the Typical Longan Genotypes from Different Origins[J].Journal of Fujian Forestry Science and Technology, 2007,34(1): 67-71,88.(in Chinese)
[8]彭宏祥, 曹辉庆, 朱建华, 等.利用 AFLP 分子标记对广西荔枝优稀种质遗传多样性及分类研究[J].西南农业学报, 2006, 9(1): 108–111.PENG H X, CAO H Q, ZHU J H, et al.Studies on the application of AFLP molecular markers on genetic diversity and classification of good and rare litchi resources in Guangxi[J].Southwest China Journalof Agricultural Science, 2006, 9(1): 108-111.(in Chinese)
[9]易干军, 谭卫萍, 霍合强, 等.龙眼品种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报, 2003,30(3): 272-276. YI G J, TAN W P, HU H Q, et al.Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP Analysis[J].Acta Horticulturae Sinica,2003,30(3): 272-276.(in Chinese)
[10]苏伟强, 黄海滨, 陆玉英, 等.龙荔与龙眼荔枝过氧化物酶同工酶分析及亲缘关系研究初报[J].广西农业科学, 1993(4): 158-159. SU W Q, HUANG H B, LU Y Y, et al.Studies on the Relationship of Longli and Longan by POD Analysis[J].Agricultural Science of Guangxi, 1993(4): 158-159.(in Chinese)
[11]ROBERT VenBUREN,et al.Longli is not a Hybrid of Longan and Lychee as Revealed by Genome Size Analysis and Trichome Morphology[J].Tropical Plant Biology, Tropical Plant Biol, 2011,4: 228-236.(in Chinese)
[12]张永福,韩丽,黄鹤平,等.荔枝、龙眼及龙荔的茎解剖结构比较[J].植物科学学报, 2014, 32(6): 551-560. ZHANG YF, HAN L, HUANG HP et al.Comparisons of Stem Anatomical Structures among Litchi, Longan and Longli[J].Plant Science Journal, 2014, 32(6): 551-560.(in Chinese)
[13]LIN T X, LIN Y, KOSHUN LSHIKI.Genetic diversity of Dimocarpus longan in China revealed by AFLP markers and partial rbcL gene sequences[J]. Scientia Horticulturae, 2005, 103(4):489-498.
[14]LIU C M, MEI M T.Classification of lychee cultivars with RAPD analysis[J].Acta Horticulturae, 2005, 665: 149-159.
[15]BUERKI S, FOREST F, ACEVEDO-RODRIGUEZ P,et al.Plastid and nuclear DNA markers reveal intricate relationships at subfamilial and tribal levels in the soapberry family (Sapindaceae) [J]. Mol Phylogenet Evol, 2009,51 (2), 238-258.
[16]何婷婷, 柴军红.基于ITS基因的部分兰属序列分析[J].分子植物育种, 2017, 15(1): 52- 57. HE T T, CAI J H.Sequence Analysis of Cymbidium Based on ITS Gene[J]. Molecular Plant Breeding, 2017, 15(1): 52- 57.(in Chinese)
[17]曹丽敏, 夏念和, 曹明, 等.中国无患子科的花粉形态及其系统学意义[J].植物科学学报, 2016, 34(6): 821-833. CAO LM, XIA NH, CAO M, et al.Pollen Morphology and Its Systematic Significance in Sapindaceae from China[J]. Plant Science Journal,2016, 34(6): 821-833.(in Chinese)
[18]PETKOVITS T, NAGY L G, HOFFMANN K, et al.Data Partitions, Bayesian Analysis and Phylogeny of the Zygomycetous Fungal Family Mortierellaceae, Inferred from Nuclear Ribosomal DNA Sequences[J]. PLoS ONE, 2011, 6(11): e27507.
[2]潘丽梅, 朱建华, 刘冰浩, 等.广西龙虎山自然保护区龙荔种群生命表分析[J].园艺学报, 2011, 38(7):1349-1355.PAN L M, ZHU J H, LIU B H, et al.Life Table Analysis of Dimocarpus confinis Population in Guangxi Longhushan Nature Reserve[J].Acta Horticulturae Sinica,2011, 38(7):1349-1355.(in Chinese)
[3]陈秀萍, 蒋际谋, 许家辉, 等.不同砧木对龙眼生长的影响试验[J].中国南方果树, 2006, 35(5):21-22.CHE X P, JIANG J M, XU J H, et al.Reseach of longan development based on different storcks[J].South China Fruits, 2006, 35(5):21-22.(in Chinese)
[4]陆贵锋, 朱松生, 黄川, 等.龙荔嫁接龙眼、荔枝的试验初报[J].中国南方果树, 2016, 45(5):148-149. LU G F, ZHU S S, HUANG C, et al.research of grafting between Longli and Longan and Litchi[J].South China Fruits, 2016, 45(5):148-149.(in Chinese)
[5]刘冰浩,徐宁,朱建华, 等.广西龙眼种质耐寒性的CTR值鉴定[J].西南农业学报, 2006, 19(4):668-671.LIU B H, XU N, ZHU J H, et al.The CTR identification of chilling-tolerance of Longan germplasm in Guangxi[J].Southwest China Journalof Agricultural Science, 2006, 19(4):668-671.(in Chinese)
[6]姜帆,高慧颖,陈秀萍, 等.龙眼属rDNA的ITS序列分析[J].果树学报, 2008, 25(2): 262- 268.JIANG F, GAO H Y, CHE X P, et al.Analysis of ITS sequences of rDNA in Dimocarpus plants[J].Journalof Fruit Science, 2008, 25(2): 262- 268.(in Chinese)
[7]高慧颖,姜帆,陈秀萍, 等.不同地域的代表性基因型龙眼RAPD分析[J].福建林业科技, 2007,34(1): 67-71,88.GAO H Y, JIANG F, CHE X P, et al.Random Amplification Polymorphic DNA (RAPD) Analysis of the Typical Longan Genotypes from Different Origins[J].Journal of Fujian Forestry Science and Technology, 2007,34(1): 67-71,88.(in Chinese)
[8]彭宏祥, 曹辉庆, 朱建华, 等.利用 AFLP 分子标记对广西荔枝优稀种质遗传多样性及分类研究[J].西南农业学报, 2006, 9(1): 108–111.PENG H X, CAO H Q, ZHU J H, et al.Studies on the application of AFLP molecular markers on genetic diversity and classification of good and rare litchi resources in Guangxi[J].Southwest China Journalof Agricultural Science, 2006, 9(1): 108-111.(in Chinese)
[9]易干军, 谭卫萍, 霍合强, 等.龙眼品种(系)遗传多样性及亲缘关系的AFLP分析[J].园艺学报, 2003,30(3): 272-276. YI G J, TAN W P, HU H Q, et al.Studies on the Genetic Diversity and Relationship of Longan Cultivars by AFLP Analysis[J].Acta Horticulturae Sinica,2003,30(3): 272-276.(in Chinese)
[10]苏伟强, 黄海滨, 陆玉英, 等.龙荔与龙眼荔枝过氧化物酶同工酶分析及亲缘关系研究初报[J].广西农业科学, 1993(4): 158-159. SU W Q, HUANG H B, LU Y Y, et al.Studies on the Relationship of Longli and Longan by POD Analysis[J].Agricultural Science of Guangxi, 1993(4): 158-159.(in Chinese)
[11]ROBERT VenBUREN,et al.Longli is not a Hybrid of Longan and Lychee as Revealed by Genome Size Analysis and Trichome Morphology[J].Tropical Plant Biology, Tropical Plant Biol, 2011,4: 228-236.(in Chinese)
[12]张永福,韩丽,黄鹤平,等.荔枝、龙眼及龙荔的茎解剖结构比较[J].植物科学学报, 2014, 32(6): 551-560. ZHANG YF, HAN L, HUANG HP et al.Comparisons of Stem Anatomical Structures among Litchi, Longan and Longli[J].Plant Science Journal, 2014, 32(6): 551-560.(in Chinese)
[13]LIN T X, LIN Y, KOSHUN LSHIKI.Genetic diversity of Dimocarpus longan in China revealed by AFLP markers and partial rbcL gene sequences[J]. Scientia Horticulturae, 2005, 103(4):489-498.
[14]LIU C M, MEI M T.Classification of lychee cultivars with RAPD analysis[J].Acta Horticulturae, 2005, 665: 149-159.
[15]BUERKI S, FOREST F, ACEVEDO-RODRIGUEZ P,et al.Plastid and nuclear DNA markers reveal intricate relationships at subfamilial and tribal levels in the soapberry family (Sapindaceae) [J]. Mol Phylogenet Evol, 2009,51 (2), 238-258.
[16]何婷婷, 柴军红.基于ITS基因的部分兰属序列分析[J].分子植物育种, 2017, 15(1): 52- 57. HE T T, CAI J H.Sequence Analysis of Cymbidium Based on ITS Gene[J]. Molecular Plant Breeding, 2017, 15(1): 52- 57.(in Chinese)
[17]曹丽敏, 夏念和, 曹明, 等.中国无患子科的花粉形态及其系统学意义[J].植物科学学报, 2016, 34(6): 821-833. CAO LM, XIA NH, CAO M, et al.Pollen Morphology and Its Systematic Significance in Sapindaceae from China[J]. Plant Science Journal,2016, 34(6): 821-833.(in Chinese)
[18]PETKOVITS T, NAGY L G, HOFFMANN K, et al.Data Partitions, Bayesian Analysis and Phylogeny of the Zygomycetous Fungal Family Mortierellaceae, Inferred from Nuclear Ribosomal DNA Sequences[J]. PLoS ONE, 2011, 6(11): e27507.
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