160份广西春大豆种质对大豆花叶病毒株系SC15和SC18的抗性评价
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摘要
广西是大豆花叶病发生较为严重的地区,大豆花叶病毒株系SC15及SC18株系是广西区内主要流行株系。为了调查广西本地大豆种质对这两个流行株系的抗性,采用人工接种的方法对160份广西本地春大豆种质进行抗性鉴定。结果显示:桂下湾灰地豆、小青豆(桂平)、小青豆-1(上思)、横县黑豆-1、横县黑豆-2、横县黑豆-3、那坡黑眼豆、隆林蛇场本地豆8份种质对SC15株系高抗,占所鉴定材料总数的5%;石塘五月黄、灌阳黄豆、凤山早黄豆、三石六月黄、两造黄豆、横县黑豆-1、田黑豆7份春大豆种质对SC18株系表现抗浸染,占所鉴定材料总数的4.37%。所鉴定的广西春大豆种质中,抗SC15株系的抗病种质抗性级别低,没有对SC15株系完全免疫的大豆种质;而抗SC18株系的大豆种质抗性级别比SC15株系高,其中有7份春大豆种质对SC18株系免疫。从抗性鉴定结果看,SC18株系对广西春大豆的致病力比SC15株系弱。因此SC15株系对大豆生产的危害更为严重,是更为值得重视的一个强毒株系。本研究筛选出的抗SMV春大豆种质可作为抗源材料用于大豆抗病育种及抗性相关的研究。
Soybean mosaic virus(SMV) is a very serious disease of soybean in its cultivation in Guangxi,where the strain SC15 and SC18 are the most prevalence strains. In order to screen for resistant materials to these two popular SMV strains,160 Guangxi spring-sowing soybean germplasms were inoculated with those two strains in two independent experiment. The results showed that 8 soybeans germplasms(Guixiawanhuididou, Guipingxiaoqingdou, Shangsixiaoqingdou-1, Hengxianheidou-1, Hengxianheidou-2, Hengxianheidou-3, Napoheiyandou, and Longlinshechangbendidou) were highly resistant to strain SC15, accounting for 5% of the tested soybean germplasms. Shitangwuyuehuang, Guanyanghuangdou, Fengshanzaohuangdou, Sanshiliuyuehuang, Liangzaohuangdou, Hengxianheidou-1, Tianheidou were resistance in infection to strain SC18, accounting for 4.37% of the tested soybean germplasms. In all those tested spring-sowing soybean germplasms, no immune germplasm was found to SC15 and the resistant rand was lower than SC18 for the resistant germplasms. There were 7 soybean germplasms immune to strain SC18. It showed that strain SC15 is virulent strain, and strain SC18 is less virulent strain, we should pay more attention to strain SC15 prevalence in soybean planting regions. Furthermore, the resistant germplasms could be used in soybean breeding, production and SMV related researches.
Soybean mosaic virus(SMV) is a very serious disease of soybean in its cultivation in Guangxi,where the strain SC15 and SC18 are the most prevalence strains. In order to screen for resistant materials to these two popular SMV strains,160 Guangxi spring-sowing soybean germplasms were inoculated with those two strains in two independent experiment. The results showed that 8 soybeans germplasms(Guixiawanhuididou, Guipingxiaoqingdou, Shangsixiaoqingdou-1, Hengxianheidou-1, Hengxianheidou-2, Hengxianheidou-3, Napoheiyandou, and Longlinshechangbendidou) were highly resistant to strain SC15, accounting for 5% of the tested soybean germplasms. Shitangwuyuehuang, Guanyanghuangdou, Fengshanzaohuangdou, Sanshiliuyuehuang, Liangzaohuangdou, Hengxianheidou-1, Tianheidou were resistance in infection to strain SC18, accounting for 4.37% of the tested soybean germplasms. In all those tested spring-sowing soybean germplasms, no immune germplasm was found to SC15 and the resistant rand was lower than SC18 for the resistant germplasms. There were 7 soybean germplasms immune to strain SC18. It showed that strain SC15 is virulent strain, and strain SC18 is less virulent strain, we should pay more attention to strain SC15 prevalence in soybean planting regions. Furthermore, the resistant germplasms could be used in soybean breeding, production and SMV related researches.
引文
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[4]孙祖东,张志鹏,蔡昭艳,等.大豆耐荫性评价体系的建立与中国南方大豆资源耐荫性变异[J].中国农业科学,2017,50(5):792-801.(Sun Z D,Zhang Z P,Cai Z Y,et al.Establishment of an evaluation system of shade tolerance in soybean and its variation in southern China germplasm population[J].Scientia Agricultura Sinica,2017,50(5):792-801.)(下转第197页)
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[6]智海剑.大豆对大豆花叶病毒抗侵染和抗扩展特性的鉴定、遗传和利用研究[D].南京:南京农业大学,2005:5-12.(Zhi HJ.Evaluation,inheritance and utilization of resistance in infection and resistance in development to soybean mosaic virus[D].Nanjing:Nanjing Agricultural University,2005:5-12.)
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[8]马莹.大豆对大豆花叶病毒抗病基因的遗传分析、精细定位和标记辅助选择研究[D].南京:南京农业大学,2010.(Ma Y.Inheritance,fine mapping and marker assisted selection of resistance genes to soybean mosaic virus in soybeans[D].Nanjing:Nanjing Agricultural University,2010.)
[9]李凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(5):670-675.(Li K,Liu Z T,Li H C,et al.Resistance to soybean mosaic virus and soybean cyst nematode of soybean cultivars from China national soybean uniform trials[J].Soybean Science,2013,32(5):670-675.)
[10]杨清华,董德坤,郁晓敏,等.浙江省大豆花叶病毒(SMV)流行株系抗性种质的筛选及农艺性状调查[J].浙江农业学报,2015,27(4):527-531.(Yang Q H,Dong D K,Yu X M,et al.Screening and agronomic character investigation of resistant germplasms to the popular strains of soybean mosaic virus(SMV)in Zhejiang province[J].Acta Agriculturae Zhejiangensis,2015,27(4):527-531.)
[11]李凯,任锐,王涛,等.大豆对大豆花叶病毒SC18株系的抗性遗传和基因定位[J].大豆科学,2017,36(2):187-191.(Li K,Ren R,Wang T,et al.Genetic analysis and mapping of soybean mosaic virus resistance genes to SC18 in soybean[J].Soybean Science,2017,36(2):187-191.)
[12]阳小凤,杨永庆,郑桂杰,等.大豆对大豆花叶病毒株系SC6和SC17抗病基因的精细定位[J].作物学报,2013,39(2):216-221.(Yang X F,Yang Y Q,Zhen G J,et al.Fine mapping of resistance genes to smv strains SC6 and SC17 in soybean[J].Acta Agronomica Sinica,2013,39(2):216-221.)
[13]邱丽娟,常汝镇,刘章雄,等.大豆种质资源描述规范和数据标准[M].北京:中国农业出版社,2006:78-79.(Qiu L J,Chang R Z,liu Z X,et al.Description specification and data standard of soybean germplasm resources[M].Beijing:Agricultural Press,2006:78-79.)
[14]智海剑,盖钧镒.大豆对大豆花叶病毒抗侵染与抗扩展育种应用的讨论[J].作物杂志,2005(3):4-6.(Zhi H J,Gai J Y.E-valuation,inheritance and utilization of resistance in infection and resistance in development to soybean mosaic virus[J].Crops,2005(3):4-6.)
[15]智海剑,盖钧镒,何小红.大豆对SMV抗侵染与抗扩展的遗传分析[J].作物学报,2005,31(10):1260-1264.(Zhi H J,Gai JY,He X H.Inheritance of resistance in infection and resistance in development to soybean mosaic virus in soybeans[J].Acta Agronomica Sinica,2005,31(10):1260-1264.)
[2]杨守臻,李初英,陈怀珠,等.广西春大豆地方品种农艺性状鉴定及聚类分析[J].广西农业科学,2005,36(1):71-74.(Yang S Z,Li C Y,Chen H Z,et al.Agronomic traits and cluster analysis of local spring soybean varieties in Guangxi[J].Guangxi Agricultural Sciences,2005,36(1):71-74.)
[3]孙祖东,杨守臻,陈怀珠,等.大豆对豆卷叶螟的抗性鉴定[J].中国油料作物学报,2005,27(4):69-71.(Sun Z D,Yang SZ,Chen H Z,et al.Identification of soybean resistance to bean pyralid(Lamprosema indicataFabricius)and oviposition preference of bean pyralid on soybean varieties[J].Chinese Journal of Oil Crop Sciences,2005,27(4):69-71.)
[4]孙祖东,张志鹏,蔡昭艳,等.大豆耐荫性评价体系的建立与中国南方大豆资源耐荫性变异[J].中国农业科学,2017,50(5):792-801.(Sun Z D,Zhang Z P,Cai Z Y,et al.Establishment of an evaluation system of shade tolerance in soybean and its variation in southern China germplasm population[J].Scientia Agricultura Sinica,2017,50(5):792-801.)(下转第197页)
[5]陈文杰,梁江,钟开珍,等.大豆抗花叶病毒材料初步筛选及评价[J].大豆科学,2012,31(4):617-620.(Chen W J,Liang J,Zhong K Z,et al.Preliminary selection and evaluation for Glycine max resistant to soybean mosaic virus[J].Soybean Science,2012,31(4):617-620.)
[6]智海剑.大豆对大豆花叶病毒抗侵染和抗扩展特性的鉴定、遗传和利用研究[D].南京:南京农业大学,2005:5-12.(Zhi HJ.Evaluation,inheritance and utilization of resistance in infection and resistance in development to soybean mosaic virus[D].Nanjing:Nanjing Agricultural University,2005:5-12.)
[7]李凯.中国南方大豆花叶病毒株系的鉴定_抗性遗传和抗性基因的定位[D].南京:南京农业大学,2009:31-58.(Li K.Strain identification of soybean mosaic virus and inheritance and gene mapping of its resistance in soybeans in southern china[D].Nanjing:Nanjing Agricultural University,2009:31-58.)
[8]马莹.大豆对大豆花叶病毒抗病基因的遗传分析、精细定位和标记辅助选择研究[D].南京:南京农业大学,2010.(Ma Y.Inheritance,fine mapping and marker assisted selection of resistance genes to soybean mosaic virus in soybeans[D].Nanjing:Nanjing Agricultural University,2010.)
[9]李凯,刘志涛,李海朝,等.国家大豆区域试验品种对SMV和SCN的抗性分析[J].大豆科学,2013,32(5):670-675.(Li K,Liu Z T,Li H C,et al.Resistance to soybean mosaic virus and soybean cyst nematode of soybean cultivars from China national soybean uniform trials[J].Soybean Science,2013,32(5):670-675.)
[10]杨清华,董德坤,郁晓敏,等.浙江省大豆花叶病毒(SMV)流行株系抗性种质的筛选及农艺性状调查[J].浙江农业学报,2015,27(4):527-531.(Yang Q H,Dong D K,Yu X M,et al.Screening and agronomic character investigation of resistant germplasms to the popular strains of soybean mosaic virus(SMV)in Zhejiang province[J].Acta Agriculturae Zhejiangensis,2015,27(4):527-531.)
[11]李凯,任锐,王涛,等.大豆对大豆花叶病毒SC18株系的抗性遗传和基因定位[J].大豆科学,2017,36(2):187-191.(Li K,Ren R,Wang T,et al.Genetic analysis and mapping of soybean mosaic virus resistance genes to SC18 in soybean[J].Soybean Science,2017,36(2):187-191.)
[12]阳小凤,杨永庆,郑桂杰,等.大豆对大豆花叶病毒株系SC6和SC17抗病基因的精细定位[J].作物学报,2013,39(2):216-221.(Yang X F,Yang Y Q,Zhen G J,et al.Fine mapping of resistance genes to smv strains SC6 and SC17 in soybean[J].Acta Agronomica Sinica,2013,39(2):216-221.)
[13]邱丽娟,常汝镇,刘章雄,等.大豆种质资源描述规范和数据标准[M].北京:中国农业出版社,2006:78-79.(Qiu L J,Chang R Z,liu Z X,et al.Description specification and data standard of soybean germplasm resources[M].Beijing:Agricultural Press,2006:78-79.)
[14]智海剑,盖钧镒.大豆对大豆花叶病毒抗侵染与抗扩展育种应用的讨论[J].作物杂志,2005(3):4-6.(Zhi H J,Gai J Y.E-valuation,inheritance and utilization of resistance in infection and resistance in development to soybean mosaic virus[J].Crops,2005(3):4-6.)
[15]智海剑,盖钧镒,何小红.大豆对SMV抗侵染与抗扩展的遗传分析[J].作物学报,2005,31(10):1260-1264.(Zhi H J,Gai JY,He X H.Inheritance of resistance in infection and resistance in development to soybean mosaic virus in soybeans[J].Acta Agronomica Sinica,2005,31(10):1260-1264.)
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