同轴扫描式实时光刺激系统
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摘要
为了解决常规选择性激活方法无法与激光扫描共焦显微镜共用扫描光路,难以与成像光路同轴的问题,提出一种同轴扫描式实时光刺激系统,使用现场可编程门阵列控制声光调制器,以调制激光扫描共焦显微镜的成像光源,经成像光路扫描后,刺激输出和成像扫描实现同步;该系统采用Xilinx KC705开发板中现场可编程门阵列集成的外设部件互连标准硬核,采用外设部件互连标准接口将上位机的刺激图像传输到现场可编程门阵列。测试结果表明,所提系统可以满足传输要求,能有效调节光刺激区域。
To solve the problem that conventional selective activation methods cannot share the scanning path in laser scanning confocal microscopy and is difficult to be coaxial with the imaging path, we design a coaxial scanning real-time light stimulus system. The field-programmable gate array controls the acousto-optic modulator to modulate the light source of the laser scanning confocal microscope. After the light path scanning, the output of stimulus can be synchronized with the imaging scanning. The system uses the peripheral component interconnect express hard core integrated by the field-programmable gate array in Xilinx KC705 development board, and uses the peripheral component interconnect express interface to transmit the stimulus image of the host computer to the field-programmable gate array. The test results show that the proposed system can satisfy the transmission requirement and adjust the light stimulus area effectively.
To solve the problem that conventional selective activation methods cannot share the scanning path in laser scanning confocal microscopy and is difficult to be coaxial with the imaging path, we design a coaxial scanning real-time light stimulus system. The field-programmable gate array controls the acousto-optic modulator to modulate the light source of the laser scanning confocal microscope. After the light path scanning, the output of stimulus can be synchronized with the imaging scanning. The system uses the peripheral component interconnect express hard core integrated by the field-programmable gate array in Xilinx KC705 development board, and uses the peripheral component interconnect express interface to transmit the stimulus image of the host computer to the field-programmable gate array. The test results show that the proposed system can satisfy the transmission requirement and adjust the light stimulus area effectively.
引文
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[10] Zheng S W. Research on a kind of CBCT image reconstruction acceleration system based on Kintex-7 board[D]. Harbin: Harbin Institute of Technology, 2014: 37-40. 郑世伟. 一种基于Kintex-7开发板的CBCT图像重建加速系统的研究[D]. 哈尔滨: 哈尔滨工业大学, 2014: 37-40.
[11] Zou C, Gao Y. Design and implementation of DMA transmission with PCIe bus based on FPGA[J]. Electronics Optics & Control, 2015, 22(7): 84-88. 邹晨, 高云. 基于FPGA的PCIe总线DMA传输的设计与实现[J]. 电光与控制, 2015, 22(7): 84-88.
[12] Zhang B, Song H J, Liu L, et al. Design of high-speed data transmission system based on PCIE interface[J]. Electronic Measurement Technology, 2015, 38(10): 113-117. 张彪, 宋红军, 刘霖, 等. 基于 PCIE 接口的高速数据传输系统设计[J]. 电子测量技术, 2015, 38(10): 113-117.
[2] Zhu Q, Yang X B, Li S M, et al. Spot array optimization of parallel confocal imaging based on digital micromirror device[J]. Acta Optica Sinica, 2018, 38(1): 0118001. 朱茜, 杨西斌, 李思黾, 等. 基于数字微镜器件并行共焦成像的光点阵列优化[J]. 光学学报, 2018, 38(1): 0118001.
[3] Kong W, Gao F, Fan J Y, et al. Application of confocal line scanning imaging technique in biomedical imaging[J]. Laser & Optoelectronics Progress, 2018, 55(5): 050003. 孔文, 高峰, 樊金宇, 等. 线扫描共聚焦成像技术在生物医学成像中的应用[J]. 激光与光电子学进展, 2018, 55(5): 050003.
[4] Qi X L, Yang T, Li L H, et al. Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme[J]. Neurophotonics, 2015, 2(4): 041406.
[5] Sakai S, Ueno K, Ishizuka T, et al. Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector[J]. Neuroscience Research, 2013, 75(1): 59-64.
[6] Papagiakoumou E, Anselmi F, Bègue A, et al. Scanless two-photon excitation of channelrhodopsin-2[J]. Nature Methods, 2010, 7(10): 848-854.
[7] Wang F X. A nerve stimulation system based on optical release of caged-compound and research[D]. Hangzhou: Zhejiang University, 2015: 10-13. 王飞翔. 基于光解笼锁的神经刺激系统研制及其实验研究[D]. 杭州: 浙江大学, 2015: 10-13.
[8] Lu X D, Zhang L J, Ou W Y. The experimental study of polarization characteristics in acousto-optic modulator[J]. Optical Technique, 2006, 32(s1): 395-397. 卢向东, 张丽娟, 欧伟英. 声光调制器偏振特性的实验研究[J]. 光学技术, 2006, 32(s1): 395-397.
[9] Xu S H, Xiao S L, Wang S, et al. Research on AOM-based modulation technology for laser communications[J]. Study on Optical Communications, 2014(5): 59-62. 徐山河, 肖沙里, 王珊, 等. 基于声光调制的激光通信调制系统研究[J]. 光通信研究, 2014(5): 59-62.
[10] Zheng S W. Research on a kind of CBCT image reconstruction acceleration system based on Kintex-7 board[D]. Harbin: Harbin Institute of Technology, 2014: 37-40. 郑世伟. 一种基于Kintex-7开发板的CBCT图像重建加速系统的研究[D]. 哈尔滨: 哈尔滨工业大学, 2014: 37-40.
[11] Zou C, Gao Y. Design and implementation of DMA transmission with PCIe bus based on FPGA[J]. Electronics Optics & Control, 2015, 22(7): 84-88. 邹晨, 高云. 基于FPGA的PCIe总线DMA传输的设计与实现[J]. 电光与控制, 2015, 22(7): 84-88.
[12] Zhang B, Song H J, Liu L, et al. Design of high-speed data transmission system based on PCIE interface[J]. Electronic Measurement Technology, 2015, 38(10): 113-117. 张彪, 宋红军, 刘霖, 等. 基于 PCIE 接口的高速数据传输系统设计[J]. 电子测量技术, 2015, 38(10): 113-117.
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