HIV抗体初筛阳性标本的复检与确证结果分析

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英文篇名：Analysis of re-inspection and confirmation for HIV-antibody preliminary screening positive samples 作者：周昀 ; 杨新智 ; 周秋颖 ; 王建华 ; 孙玉萍 ; 杨政权 英文作者：ZHOU Yun;YANG Xinzhi;ZHOU Qiuying;WANG Jianhua;SUN Yuping;YANG Zhengquan;Dandong Disease Prevention and Control Center; 关键词：HIV抗体 ; 筛查阳性 ; 确证试验 ; Western ; blot法 英文关键词：HIV antibody;;screening positive;;confirmatory testing;;Western blot 中文刊名：SYYX 英文刊名：Journal of Shenyang Medical College 机构：丹东市疾病预防控制中心; 出版日期：2019-01-15 出版单位：沈阳医学院学报 年：2019 期：v.21;No.87 语种：中文; 页：SYYX201901018 页数：4 CN：01 ISSN：21-1393/R 分类号：62-64+67

摘要

目的:探讨HIV抗体初筛阳性标本的复检与确证试验结果之间的关系。方法:对2011年1月至2017年10月HIV抗体初筛阳性标本按照《全国艾滋病检测技术规范(2009年、2015年修订版)》要求分别采用酶联免疫吸附试验(ELISA)和胶体硒法进行复检,复检任一方法阳性采用Western blot法进行确证,收集数据进行分析。结果:656份HIV抗体初筛阳性标本,622份复检阳性,初筛假阳性率为5.2%(34/656)。其中受检者信息齐全的490份标本进行确证试验,确证阳性标本450份(91.8%),阴性11份(2.3%),不确定29份(5.9%)。ELISA法复检阳性标本确证阳性率为92.9%(450/488),胶体硒法复检阳性标本确证阳性率为99.1%(450/454),差异有统计学意义(χ~2=31.22,P<0.01);ELISA法中S/CO值>6组与3≤S/CO值≤6组和1≤S/CO值<3组的确证阳性率比较差异均有统计学意义(χ~2=96.33、301.15,P<0.01);ELISA法S/CO值与确证阳性标本出现条带数量呈正相关,Gamma系数为0.788。结论:采用优先选择胶体硒法+ELISA法补充的模式进行复检可以大大提高检测效率,保证及时有效地发现HIV感染者。较高S/CO值的标本感染HIV的可能性较大,且病毒处于复制活跃期具有较强感染性的可能性也较大。
        Objective:To analyze the correlation between the re-inspection and confirmation for HIV-antibody preliminary screening positive samples. Method:ELISA and colloidal selenium assay were used to re-inspect preliminarily screening positive samples from Jan 2011 to Oct 2017,then the suspicious re-inspection positive samples were confirmed by Western blot. Results:There were 656 HIV-antibody preliminary screening positive samples in total,and 622 re-inspection samples were positive. The false positive rate of HIV-antibody preliminary screening was 5.2%(34/656). And 490 samples with complete information of the subjects were confirmed by Western blot,and 450 samples were positive(91.8%),11 samples were negative(2.3%),and 29 samples were uncertain(5.9%). The confirmed positive rate was 92.9%(450/488)in samples re-inspected by ELISA,and th econfirmed positive rate was 99.1%(450/454)in samples re-inspected by colloidal selenium assay(P<0.01). Compared with the S/CO>6 group,the confirmed positive rates of the 3≤ S/CO≤ 6 group and 1≤ S/CO<3 group in samples re-inspected by ELISA were significantly lower(P<0.01). The S/CO value was positively correlated with the number of bands of confirmed positive samples(P<0.01). Conclusion:Application of colloidal selenium assay combined with ELISA can greatly increase the confirmed positive rate.

引文

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