响应面法优化郁柴茯神颗粒剂的提取工艺
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摘要
目的优选郁柴茯神颗粒剂的提取工艺。方法以浸出物得率和HPLC法检测的2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量为综合考察指标,单因素筛选提取工艺参数,考察回流时间、乙醇体积分数、乙醇用量和提取次数4个因素,利用Box-Behnken中心组合实验和Design-Expert 8.0.6.1软件统计分析,进一步优化郁柴茯神颗粒剂的提取工艺条件。结果确定郁柴茯神颗粒剂的最佳提取工艺条件为:回流时间为3.0 h,乙醇用量为6倍,乙醇体积分数为80%,提取2次。结论采用响应面法优化郁柴茯神颗粒剂的提取工艺,实际值与预测值吻合度良好,研究结果为郁柴茯神颗粒剂的研究开发提供参考依据。
Objective To optimize the extraction process of Yuchaifushen Granules.Methods The extraction yield and HPLC method for the determination of 2,3,5,4′-tetrahydroxydistyrene-2-O-β-D-glucoside content were used as the comprehensive evaluation indexes,and the parameters were selected by single factor screening,and 4 factors such as reflux time,ethanol volume fraction,alcohol consumption and extraction times were investigated.Box-Behnken center combined test and software Design-Expert 8.0.6.1 were used to further optimize the extraction conditions of Yuchaifushen Granules.Results The optimum extraction conditions of Yuchaifushen Granules were as follows:the reflux time was 3.0 h,the ethanol dosage was 6 times,and the ethanol volume fraction was 80% with extraction 2 times.Conclusion The response surface method was used to optimize the extraction process of Yuchai fushen Granules.The actual values were in good agreement with the predicted values,and the research results provide reference for the research and development of Yuchaifushen Granules.
Objective To optimize the extraction process of Yuchaifushen Granules.Methods The extraction yield and HPLC method for the determination of 2,3,5,4′-tetrahydroxydistyrene-2-O-β-D-glucoside content were used as the comprehensive evaluation indexes,and the parameters were selected by single factor screening,and 4 factors such as reflux time,ethanol volume fraction,alcohol consumption and extraction times were investigated.Box-Behnken center combined test and software Design-Expert 8.0.6.1 were used to further optimize the extraction conditions of Yuchaifushen Granules.Results The optimum extraction conditions of Yuchaifushen Granules were as follows:the reflux time was 3.0 h,the ethanol dosage was 6 times,and the ethanol volume fraction was 80% with extraction 2 times.Conclusion The response surface method was used to optimize the extraction process of Yuchai fushen Granules.The actual values were in good agreement with the predicted values,and the research results provide reference for the research and development of Yuchaifushen Granules.
引文
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[14] 江连洲,王海晴,陈思,等.响应面法优化超声波辅助水酶法提取牡丹籽油工艺研究[J].食品工业科技,2016,37(8):247-251.
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[16] 确生,王志波,李成思,等.响应面法优化斑花黄堇中总生物碱的提取工艺[J].中国现代应用药学,2017,34(3):358-362.
[17] 张占军,葛洪,郑倩倩,等.响应面法优化热水浸提啤酒花多糖的工艺研究[J].食品研究与开发,2015,36(2):30-36.
[18] 古丽巴哈尔·卡吾力,常占瑛,哈及尼沙,等.黑果枸杞花青素的提取工艺及抗氧化活性研究[J].西北药学杂志,2017,32(4):455-460.
[19] 王永菲,王成国.响应面法的理论与应用[J].中央民族大学学报:自然科学版,2005,14(3):236-240.
[2] 孙从永.助眠天然活性成分丁香酸脂质体的制备及体内外评价研究[D].镇江:江苏大学,2017.
[3] 国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:265-267.
[4] Lin L,Ni B,Lin H,et al.Simultaneous determination and pharmacokinetic study of P-hydroxybenzaldehyde,2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside,emodin-8-O-β-D-glucopyranoside,and emodin in rat plasma by liquid chromatography tandem mass spectrometry after oral administration of Polygonum multiflorum[J].Anal Methods,2014,7(1):244-252.
[5] 王付荣,周洪雷.何首乌及夜交藤化学成分及药理作用的研究进展[J].江西中医学院学报,2007,19(5):98-100.
[6] Lin L,Ni B,Lin H,et al.Traditional usages,botany,phytochemistry,pharmacology and toxicology of Polygonum multiflorum Thunb.:a review[J].J Ethnopharmacol,2015,159:158-183.
[7] 白璐,贺文娟,崔春利,等.Box-Behnken响应面法优化延胡索配方颗粒的提取工艺[J].西北药学杂志,2013,28(1):1-4.
[8] 武婧,宋忠兴,古川,等.星点设计-响应面法优化黄柏提取工艺研究[J].中国现代中药,2015,17(9):953-956.
[9] 刘长龙,李云霄,钱俊,等.响应面法优化十味盆安颗粒虎杖醋延胡索的提取工艺研究[J].世界科学技术-中医药现代化,2016,18(9):1602-1608.
[10] 海妮·巴音达,阿不都拉·阿巴斯.响应面试验优化紫薯皮色素提取工艺及其稳定性分析[J].食品科学,2016,37(14):56-61.
[11] 单鸣秋,钱岩,于生,等.基于响应面法的天麻产地加工炮制一体化工艺研究[J].中草药,2016,47(3):420-424.
[12] 李存玉,马赟,龚柔佳,等.响应面分析法耦合调节Donnan效应优化苦参提取液的纳滤浓缩工艺[J].中草药,2016,47(19):3395-3400.
[13] 刘丹,何凤军,刘长龙,等.响应面法优选妇科Ⅳ颗粒一步制粒工艺[J].中国中药杂志,2016,41(1):75-82.
[14] 江连洲,王海晴,陈思,等.响应面法优化超声波辅助水酶法提取牡丹籽油工艺研究[J].食品工业科技,2016,37(8):247-251.
[15] 施政,王建平,章建军,等.响应面法优化穿山龙总皂苷提取工艺[J].中国现代应用药学,2017,34(1):68-71.
[16] 确生,王志波,李成思,等.响应面法优化斑花黄堇中总生物碱的提取工艺[J].中国现代应用药学,2017,34(3):358-362.
[17] 张占军,葛洪,郑倩倩,等.响应面法优化热水浸提啤酒花多糖的工艺研究[J].食品研究与开发,2015,36(2):30-36.
[18] 古丽巴哈尔·卡吾力,常占瑛,哈及尼沙,等.黑果枸杞花青素的提取工艺及抗氧化活性研究[J].西北药学杂志,2017,32(4):455-460.
[19] 王永菲,王成国.响应面法的理论与应用[J].中央民族大学学报:自然科学版,2005,14(3):236-240.
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