2016年-2017年上海市金山区HIV筛查阳性标本确证实验结果及带型分析
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摘要
目的对上海市金山区2016年-2017年667份初筛阳性样本进行确证实验,对确证阳性样本进行免疫印迹试验(WB)条带分析,提高实验室HIV抗体检测能力,增强对WB实验结果的分析判断能力。方法对金山区各艾滋病筛查实验室送检的HIV抗体初筛阳性样品,WB确证检测。结果 667份筛查阳性标本WB阳性482份(72.26%),不确定84份(12.59%),阴性101份(15.14%);确证阳性病例主要来自自愿咨询检测和医疗机构就诊检测,不同送检来源的样品阳性率差异无统计学意义(P>0.05);确证阳性样品带型以全带型及次全带型为主。结论 HIV感染的确证依赖于WB实验,WB带型的阳性判读应严格按照《全国艾滋病检测技术规范》(2015版)及试剂盒说明书,参考实际情况,谨慎判定;随着宣传力度的加大,艾滋病的自愿咨询检测得以推广,艾滋病自愿咨询检测及医疗机构检测已经成为发现HIV感染者的主要方式。
Objective To perform WB banding pattern analysis on 667 screening positive samples in Jinshan District, Shanghai during 2016-2017, improve the capacity of laboratory HIV antibody detection, and enhance the ability of analyzing and judging WB experimental results. Methods To perform Western blot(WB) on HIV antibody screening positive samples in Jinshan District. Results There were 482 WB confirmed positive samples in 667 screening positive samples, accounting for 72.26%, and 84 samples were uncertain, accounting for 12.59%; 101 samples were negative, accounting for 15.14%. Confirmed positive cases mainly came from voluntary counseling and medical institutions. There was no statistical significance on the difference in the positive rate of samples from different sources(P>0.05). The major banding patterns of WB confirmed positive samples were the complete banding pattern and the sub-complete banding patterns. Conclusion The diagnosis of HIV depends on WB test. Positive judgment of WB banding pattern should be strictly in accordance with the National HIV Testing Technical Specifications(2015) and judgment should be made carefully by binding the reagent specification and referring to the actual situation. More and more people know the voluntary counseling and testing(VCT) with the increase of propaganda, and it has been a mainly way to find the infection of HIV.
Objective To perform WB banding pattern analysis on 667 screening positive samples in Jinshan District, Shanghai during 2016-2017, improve the capacity of laboratory HIV antibody detection, and enhance the ability of analyzing and judging WB experimental results. Methods To perform Western blot(WB) on HIV antibody screening positive samples in Jinshan District. Results There were 482 WB confirmed positive samples in 667 screening positive samples, accounting for 72.26%, and 84 samples were uncertain, accounting for 12.59%; 101 samples were negative, accounting for 15.14%. Confirmed positive cases mainly came from voluntary counseling and medical institutions. There was no statistical significance on the difference in the positive rate of samples from different sources(P>0.05). The major banding patterns of WB confirmed positive samples were the complete banding pattern and the sub-complete banding patterns. Conclusion The diagnosis of HIV depends on WB test. Positive judgment of WB banding pattern should be strictly in accordance with the National HIV Testing Technical Specifications(2015) and judgment should be made carefully by binding the reagent specification and referring to the actual situation. More and more people know the voluntary counseling and testing(VCT) with the increase of propaganda, and it has been a mainly way to find the infection of HIV.
引文
[1] 中国疾病预防控制中心. 全国艾滋病检测技术规范(2015年修订版)[Z]. 2015-12.
[2] 张丽中, 姜焕荣, 王瑞雪, 等. 127例艾滋病病毒感染者/艾滋病患者检测结果分析[J]. 中国药物与临床, 2017, 17(12): 1841-1844.
[3] 刘建敏, 董雪, 李欣, 等. 沈阳市2013 年HIV 抗体初筛阳性标本的复检及确证结果分析[J]. 中国卫生检验杂志, 2015, 25(6): 844-851.
[4] 周雯婧, 任智晶, 叶震璇, 等. 贵州地区2013～2015年HIV抗体筛查及确证结果分析[J].检验医学与临床, 2016, 13(15): 2141-2143.
[5] 王印, 李在村, 代丽丽. HIV感染的诊断、治疗及预防[J]. 中国血液净化, 2017, 16(10): 651-671.
[6] 王险峰, 李峰, 刘晓松, 等. 2013-2015年石家庄市HIV确证结果分析[J]. 江苏预防医学, 2017, 28(2): 163-165.
[7] 崔海燕, 薛秀娟, 刘春华, 等. HIV抗体确证试验不确定结果的解析[J]. 中国艾滋病性病, 2017, 23(7): 596-598.
[8] 王颖, 李一苇. 菏泽市蛋白印迹试验HIV-1抗体不确定结果分析[J]. 中国艾滋病性病, 2016, 22(16): 911-913.
[9] 龙春平, 彭瑾瑜, 张昌芝, 等. 湘西自治州HIV抗体免疫印迹试验不确定结果分析[J]. 中国卫生检验杂志, 2018, 28(9): 1054-1056.
[10] 韩燕, 李玄, 魏志刚. 咸阳市2010-2015年HIV抗体不确定结果分析[J]. 中国艾滋病性病, 2016, 22(10): 831-833.
[11] 廖文兰, 郑禄祥, 阙金财, 等. 龙岩市2012-2016年艾滋病流行特征[J]. 海峡预防医学杂志, 2017, 23(6): 24-26.
[12] 郑禄祥, 林梅艳, 邓小如, 等. 龙岩市2014-2015年艾滋病异性传播危险因素调查[J]. 海峡预防医学杂志, 2016, 22(4): 36-37.
[13] 潘珂君, 斯热吉木·依明, 古丽赛娜木·艾合买提, 等. 1761名VCT者人口学特征及结果分析[J]. 中国艾滋病性病, 2015, 21(3): 223-224.
[14] 安红波. 多部门合作开展VCT综合服务模式探讨[J]. 内蒙古中医药, 2013, 32(28): 86-86.
[15] 高娟, 严钏元, 田辉. 宝鸡市某VCT门诊6年间求询者HIV感染情况及影响因素分析[J].中国健康教育, 2017, 33(11): 1008-1010.
[2] 张丽中, 姜焕荣, 王瑞雪, 等. 127例艾滋病病毒感染者/艾滋病患者检测结果分析[J]. 中国药物与临床, 2017, 17(12): 1841-1844.
[3] 刘建敏, 董雪, 李欣, 等. 沈阳市2013 年HIV 抗体初筛阳性标本的复检及确证结果分析[J]. 中国卫生检验杂志, 2015, 25(6): 844-851.
[4] 周雯婧, 任智晶, 叶震璇, 等. 贵州地区2013～2015年HIV抗体筛查及确证结果分析[J].检验医学与临床, 2016, 13(15): 2141-2143.
[5] 王印, 李在村, 代丽丽. HIV感染的诊断、治疗及预防[J]. 中国血液净化, 2017, 16(10): 651-671.
[6] 王险峰, 李峰, 刘晓松, 等. 2013-2015年石家庄市HIV确证结果分析[J]. 江苏预防医学, 2017, 28(2): 163-165.
[7] 崔海燕, 薛秀娟, 刘春华, 等. HIV抗体确证试验不确定结果的解析[J]. 中国艾滋病性病, 2017, 23(7): 596-598.
[8] 王颖, 李一苇. 菏泽市蛋白印迹试验HIV-1抗体不确定结果分析[J]. 中国艾滋病性病, 2016, 22(16): 911-913.
[9] 龙春平, 彭瑾瑜, 张昌芝, 等. 湘西自治州HIV抗体免疫印迹试验不确定结果分析[J]. 中国卫生检验杂志, 2018, 28(9): 1054-1056.
[10] 韩燕, 李玄, 魏志刚. 咸阳市2010-2015年HIV抗体不确定结果分析[J]. 中国艾滋病性病, 2016, 22(10): 831-833.
[11] 廖文兰, 郑禄祥, 阙金财, 等. 龙岩市2012-2016年艾滋病流行特征[J]. 海峡预防医学杂志, 2017, 23(6): 24-26.
[12] 郑禄祥, 林梅艳, 邓小如, 等. 龙岩市2014-2015年艾滋病异性传播危险因素调查[J]. 海峡预防医学杂志, 2016, 22(4): 36-37.
[13] 潘珂君, 斯热吉木·依明, 古丽赛娜木·艾合买提, 等. 1761名VCT者人口学特征及结果分析[J]. 中国艾滋病性病, 2015, 21(3): 223-224.
[14] 安红波. 多部门合作开展VCT综合服务模式探讨[J]. 内蒙古中医药, 2013, 32(28): 86-86.
[15] 高娟, 严钏元, 田辉. 宝鸡市某VCT门诊6年间求询者HIV感染情况及影响因素分析[J].中国健康教育, 2017, 33(11): 1008-1010.
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