淋病奈瑟菌选择性固体培养基性能评估
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摘要
目的对4种商品化淋病奈瑟菌选择性固体培养基(TM培养基)进行性能评估。方法参照《培养基制备指南》及《淋球菌琼脂基础培养基》推荐方法,用生长率、选择性和微生物污染,以及结合临床应用效果,综合评价4种商品化TM培养基的检测性能。结果待测目标菌在进口厂商1(梅里埃)、国产厂商2、3、4TM培养基上的生长率(PR)分别为0.84、0.45、0.30和0.05,其中厂商4 PR低于规定下限,不同厂商之间TM培养基的PR比较,差异均有统计学意义(P<0.05)。4厂商TM培养基对非目标菌的选择因子(SF)为2～5之间,均符合规定。各厂商TM培养基的微生物污染率符合要求(均为0)。调查临床应用厂商1、2、3、4TM培养基的淋病奈瑟菌检出率分别为17.6%、17.0%、7.3%、8.5%,其与相应厂商培养基的PR有较好的一致性。培养基杂菌生长测试,厂商4培养基对真菌防污染能力弱,其他厂商均符合要求。结论市售淋病奈瑟菌固体选择性培养基的质量存在品牌间差异,选用时可通过性能测试选择优质的培养基,以提高临床样本淋病奈瑟菌的检出率。
Objective To evaluate the performance of four commercial selective media(Thayer-Martin medium,TM)for Neisseria gonorrhoeae.Methods According to the guidelines for the preparation of media and the recommended method of N.Gonorrhoeae agar base medium preparation,the performance of four commercial TM media was comprehensively evaluated by growth rate,selectivity,microbial contamination and clinical application.Results The growth rate(PR)of the tested bacteria on BioMerieux medium and other three domestic manufacturer media were 0.84,0.45,0.30 and 0.05 respectively.Among them,the rate of PRof manufacturer 4 was lower than that of the prescribed lower limit.The PRof TM medium was significantly different among different manufacturers(P<0.05).The selection factor(SF)of all the four TM media for non-target bacteria was from 2 to 5,which met the requirements.The microbial contamination rates of all the tested TM media met the requirements(0).The detection rates of the 1,2,3 and 4 TM media in the clinical settings were 17.6%,17.0%,7.3%and 8.5%respectively,which were in good agreement with the PRof the corresponding manufacturers.The non-target fungi growth test showed that the anti-contamination ability of manufacturer 4 was weak,and other manufacturers met the requirements.Conclusion The quality of the N.gonorrhoeae selective media varies from brand to brand.The high-quality media should be selected by performance test to improve the detection rate of N.gonorrhoeae in clinical settings.
Objective To evaluate the performance of four commercial selective media(Thayer-Martin medium,TM)for Neisseria gonorrhoeae.Methods According to the guidelines for the preparation of media and the recommended method of N.Gonorrhoeae agar base medium preparation,the performance of four commercial TM media was comprehensively evaluated by growth rate,selectivity,microbial contamination and clinical application.Results The growth rate(PR)of the tested bacteria on BioMerieux medium and other three domestic manufacturer media were 0.84,0.45,0.30 and 0.05 respectively.Among them,the rate of PRof manufacturer 4 was lower than that of the prescribed lower limit.The PRof TM medium was significantly different among different manufacturers(P<0.05).The selection factor(SF)of all the four TM media for non-target bacteria was from 2 to 5,which met the requirements.The microbial contamination rates of all the tested TM media met the requirements(0).The detection rates of the 1,2,3 and 4 TM media in the clinical settings were 17.6%,17.0%,7.3%and 8.5%respectively,which were in good agreement with the PRof the corresponding manufacturers.The non-target fungi growth test showed that the anti-contamination ability of manufacturer 4 was weak,and other manufacturers met the requirements.Conclusion The quality of the N.gonorrhoeae selective media varies from brand to brand.The high-quality media should be selected by performance test to improve the detection rate of N.gonorrhoeae in clinical settings.
引文
[1]中国疾病预防控制中心性病艾滋病预防控制中心性病控制中心.2016年12月全国艾滋病性病疫情[J].中国艾滋病性病,2017,24(2):93.
[2]李正添,熊明洲,王晓华,等.2008-2016年中山市性病疫情的流行病学特征分析[J].现代预防医学,2018,45(3):409-411.
[3]穆金萍.2012-2016年沈阳市淋病发病趋势与流行特征分析[J].现代预防医学,2018,45(3):401-404.
[4]Aexandra R,Corinna M,Petra M,et al.Innate recognition by neutrophil granulocytes differs between Neisseria gonorrhoeae strains causing local or disseminating infections[J].Infect Immun,2013,81(7):2358-2370.
[5]彭端亮,李永文,赵鹃.淋病奈瑟菌、沙眼衣原体、解脲脲支原体感染与女性不孕症的关系[J].中华医院感染学杂志,2015,25(23):5494-5496.
[6]Chen YC,Liu HY,Li CY,et al.The Rising Trend of Sexually Transmitted Infections Among HIV-Infected Persons:A Population-Based Cohort Study in Taiwan,2000 Through 2010[J].JAIDS Journal of Acquired Immune Deficiency Syndromes,2015,68(4):432-438.
[7]Sanders EJ,Wahome E,Okuku HS,et al.Evaluation of who screening algorithm for the presumptive treatment of asymptomatic rectal gonorrhea and Chlamydia infections in at-risk MSM in Kenya[J].Sex Transm Infect,2014,90(2):94-99.
[8]朱邦勇,李伟,梁铭,等.纸片扩散法检测淋病奈瑟菌对头孢菌素类药物敏感性的准确率分析[J].中国艾滋病性病,2016,22(8):651-653.
[9]刘小凤,沈守星,魏秋姣.广东省珠海地区2010-2015年淋球菌耐药监测变化趋势分析[J].皮肤性病诊疗学杂志,2016,23(6):389-392.
[10]张文辉,杨毅.性病和皮肤病检验诊断[M].北京:人民卫生出版社,2016:84-103.
[11]郑麟毅,吴文凡,陈舒奕,等.SNT 1538.2-2016,培养基制备指南第2部分:培养基性能测试实用指南[S].北京:国家质量监督检验检疫总局,2016.
[12]刘艳,孙彬裕,曲守方,等.YYT 1166-2009,淋球菌琼脂基础培养基[S].北京:中国医药科技出版社,2009.
[13]容艳芬,帅惠萍,朱海明,等.肠道致病菌选择性固体培养基质量性能评价方法的探讨[J].中国卫生检验杂志,2013,23(6):1447-1480.
[14]何天文,陈亮亮,卢勉飞,等.三种创伤弧菌分离培养基的性能测试研究[J].中国卫生检验杂志,2013,23(6):1469-1472.
[15]黄晓雯,赵虎,张敏,等.淋病奈瑟菌耐药性调查及耐药基因的定位与转移研究[J].扬州大学学报,2015,36(2):11-13.
[16]查则瑾,陈林龙,裘珍珍,等.阴道与宫颈分泌物细菌培养及药敏分析[J].中华医院感染学杂志,2013,23(23):5883-5885.
[2]李正添,熊明洲,王晓华,等.2008-2016年中山市性病疫情的流行病学特征分析[J].现代预防医学,2018,45(3):409-411.
[3]穆金萍.2012-2016年沈阳市淋病发病趋势与流行特征分析[J].现代预防医学,2018,45(3):401-404.
[4]Aexandra R,Corinna M,Petra M,et al.Innate recognition by neutrophil granulocytes differs between Neisseria gonorrhoeae strains causing local or disseminating infections[J].Infect Immun,2013,81(7):2358-2370.
[5]彭端亮,李永文,赵鹃.淋病奈瑟菌、沙眼衣原体、解脲脲支原体感染与女性不孕症的关系[J].中华医院感染学杂志,2015,25(23):5494-5496.
[6]Chen YC,Liu HY,Li CY,et al.The Rising Trend of Sexually Transmitted Infections Among HIV-Infected Persons:A Population-Based Cohort Study in Taiwan,2000 Through 2010[J].JAIDS Journal of Acquired Immune Deficiency Syndromes,2015,68(4):432-438.
[7]Sanders EJ,Wahome E,Okuku HS,et al.Evaluation of who screening algorithm for the presumptive treatment of asymptomatic rectal gonorrhea and Chlamydia infections in at-risk MSM in Kenya[J].Sex Transm Infect,2014,90(2):94-99.
[8]朱邦勇,李伟,梁铭,等.纸片扩散法检测淋病奈瑟菌对头孢菌素类药物敏感性的准确率分析[J].中国艾滋病性病,2016,22(8):651-653.
[9]刘小凤,沈守星,魏秋姣.广东省珠海地区2010-2015年淋球菌耐药监测变化趋势分析[J].皮肤性病诊疗学杂志,2016,23(6):389-392.
[10]张文辉,杨毅.性病和皮肤病检验诊断[M].北京:人民卫生出版社,2016:84-103.
[11]郑麟毅,吴文凡,陈舒奕,等.SNT 1538.2-2016,培养基制备指南第2部分:培养基性能测试实用指南[S].北京:国家质量监督检验检疫总局,2016.
[12]刘艳,孙彬裕,曲守方,等.YYT 1166-2009,淋球菌琼脂基础培养基[S].北京:中国医药科技出版社,2009.
[13]容艳芬,帅惠萍,朱海明,等.肠道致病菌选择性固体培养基质量性能评价方法的探讨[J].中国卫生检验杂志,2013,23(6):1447-1480.
[14]何天文,陈亮亮,卢勉飞,等.三种创伤弧菌分离培养基的性能测试研究[J].中国卫生检验杂志,2013,23(6):1469-1472.
[15]黄晓雯,赵虎,张敏,等.淋病奈瑟菌耐药性调查及耐药基因的定位与转移研究[J].扬州大学学报,2015,36(2):11-13.
[16]查则瑾,陈林龙,裘珍珍,等.阴道与宫颈分泌物细菌培养及药敏分析[J].中华医院感染学杂志,2013,23(23):5883-5885.
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