海洋性弧菌致病机制研究进展
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摘要
弧菌是一种革兰阴性菌,广泛分布于自然界的水中。弧菌是引起人类和水生动物细菌性感染的主要病原体之一,人类食用被污染的食物或伤口直接接触被污染的水体环境,会引起腹泻、伤口感染甚至败血症等疾病。随着我国海产品养殖产业的蓬勃发展,海洋性致病性弧菌感染所致疾病的发病率越来越高,探索其致病机制刻不容缓。弧菌的致病力与其毒力因子密切相关,主要的毒力因子包括溶血素、铁过载和内、外毒素等。本文主要针对几种常见的海洋性弧菌(创伤弧菌、溶藻弧菌、霍乱弧菌、副溶血性弧菌)进行探讨,分别从其生物学性状、所致疾病及致病因子生物机制3个方面展开综述,以期为海洋性弧菌致病机制和防治提供借鉴和参考。
Vibrio is a gram-negative bacterium, which is widely distributed in natural water. Vibrio is one of the major pathogens which could cause bacterial infections in humans and aquatic animals. Human consumption of contaminated food or wounds directly exposed to contaminated water environment can cause diarrhea, wound infection and even septicemia. With the vigorous development of China's marine aquaculture industry, the incidence of diseases caused by marine Vibrio infection is increasing. It is urgent to explore its pathogenic mechanism. The virulence of Vibrio is closely related to its virulence factors, including hemolysin, iron overload, endotoxin and exotoxin. The article summarizes research development of main marine Vibrios(Vibrio vulnificus, Vibrio alginolyticus, Vibrio cholerae and Vibrio parahaemolyticus) from their biological characters, related diseases,and Pathogenic Factors and pathogenic molecular mechanism, in order to provide reference for pathogenic mechanism and prevention of marine Vibrio.
Vibrio is a gram-negative bacterium, which is widely distributed in natural water. Vibrio is one of the major pathogens which could cause bacterial infections in humans and aquatic animals. Human consumption of contaminated food or wounds directly exposed to contaminated water environment can cause diarrhea, wound infection and even septicemia. With the vigorous development of China's marine aquaculture industry, the incidence of diseases caused by marine Vibrio infection is increasing. It is urgent to explore its pathogenic mechanism. The virulence of Vibrio is closely related to its virulence factors, including hemolysin, iron overload, endotoxin and exotoxin. The article summarizes research development of main marine Vibrios(Vibrio vulnificus, Vibrio alginolyticus, Vibrio cholerae and Vibrio parahaemolyticus) from their biological characters, related diseases,and Pathogenic Factors and pathogenic molecular mechanism, in order to provide reference for pathogenic mechanism and prevention of marine Vibrio.
引文
[1]武静,刘晓斐,胡成进.创伤弧菌流行病学调查及致病机制研究现状[J].医学研究杂志,2015,44(3):166-168.
[2]邢丽萍,周虓.创伤弧菌生物学研究进展[J].中国卫生检验杂志,2011,21(7):1833-1836.
[3]JEONG H,SATCHELL K. Additive function of Vibrio vulnificus MARTX(Vv)and VvhA cytolysins promotes rapid growth and epithelial tissue necrosisduringintestinalinfection[J].PLoSPathog,2012,8(3):e1002581.
[4]叶淑瑶,杨保伟,李凤琴.创伤弧菌致病性及致病机制研究进展[J].中国食品卫生杂志,2018,30(2):213-219.
[5] KWON K,YANG J,RYU D,et al. Vibrio vulnificus cytolysin induces superoxide anion-initiated apoptotic signaling pathway in human ECV304 cells[J]. J Biol Chem,2001,276(50):47518-47523.
[6]李桂军,楼永良.创伤弧菌细胞素致病机制的研究进展[J].国际检验医学杂志,2006(7):633-635.
[7]MIYOSHI S,NARUKAWA H,TOMOCHIKA K,et al. Actions of Vibrio vulnificus metalloprotease on human plasma proteinase-proteinase inhibitor systems:a comparative study of native protease with its derivative modified by polyethylene glycol[J]. Microbiol Immunol,1995,39(12):959-966.
[8]滕勇勇,王琪,吴雷,等.致病性弧菌的生物学特性和致病因子研究进展[J].热带医学杂志,2014,14(10):1396-1399.
[9]DATTA S,CROSA JH. Identification and characterization of a novel outer membrane protein receptor required for hemin utilization in Vibrio vulnificus[J]. Biometals,2012,25(2):275-283.
[10]PAJUELO D,HERNANDEZ-CABANYERO C,SAJUAN E,et al.Iron and Fur in the life cycle of the zoonotic pathogen Vibrio vulnificus[J]. Environ Microbiol,2016,18(11):4005-4022.
[11]KIM I,SHIM J,LEE K,et al. Nonribosomal peptide synthase is responsible for the biosynthesis of siderophore in Vibrio vulnificus MO6-24/O[J]. J Microbiol Biotechnol,2008,18(1):35-42.
[12]HILTON T,ROSCHE T,FROELICH B,et al. Capsular polysaccharide phase variation in Vibrio vulnificus[J]. Appl Environ Microbiol,2006,72(11):6986-6993.
[13]郑晨晖.黄姑鱼源溶藻弧菌的分离鉴定[J].北京农业,2014(27):7-9.
[14]彭喜春,张宁,冉艳红,等.海产品中溶藻弧菌的筛选及其致病因子的研究[J].现代食品科技,2008(4):312-315.
[15]BAFFONE W,CITTERIO B,VITTORIA E,et al. Determination of several potential virulence factors in Vibrio spp isolated from seawater[J/OL].Fod Microbiology,2001(18):479-488.[2018-10-29].https://www.idealibrary.com on.DOI:10.1006/fmic.2001.0441
[16]鄢庆枇,苏永全,王军.溶藻弧菌脂多糖对大黄鱼的毒性与免疫保护性试验[J].台湾海峡,2003,22(2):163-167.
[17]BALEBONA M,ANDREU M,BORDAS M,et al. Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream(Sparus aurata L.)[J].Appl Environ Microbiol,1998,64(11):4269-4275.
[18]苑淑宾,朱爱意.溶藻弧菌对水产动物致病性及其防治的研究进展[J].浙江海洋学院学报(自然科学版),2012,31(3):256-264.
[19]BELAS M,COLWELL R. Adsorption kinetics of laterally and polarly flagellated Vibrio[J]. J Bacteriol,1982,151(3):1568-1580.
[20]BORDAS M,BALEBONA M,ZOEEILLA I,et al. Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream(Sparus aurata L.)[J].Appl Environ Microbiol,1996,62(10):3650-3654.
[21]王蓬勃,马悦,刘琴.溶藻弧菌铁载体合成及外膜蛋白表达的研究[J].微生物学报,2006,33(2):48-53.
[22]梅冰,陆翔,王丽娜,等.溶藻弧菌的毒力因子与相关基因的研究进展[J].辽宁农业科学,2015(5):58-60.
[23]毕振强,赵仲堂.霍乱弧菌致病因子及调控基因研究进展[J].中国公共卫生,2005,21(6):754-755.
[24]TAYLOR R,KIRN T,MEEKS M,et al. A Vibrio cholerae classical TcpA amino acid sequence induces protective antibody that binds an area hypothesized to be important for toxin-coregulated pilus structure[J].Infect Immun,2004,72(10):6050-6060.
[25]KARAOLIS D,SOMARA S,MANEVAL D,et al. A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria[J].Nature,1999,399(6734):375-379.
[26]王红.副溶血性弧菌传播流行及分子检测技术概述[J].大众科技,2018,20(4):46-48,53.
[27] HAMADA D,HIGURASHI T,MAYANAGI K,et al. Tetrameric structure of thermostable direct hemolysin from vibrio parahaemolyticus revealed by ultracentrifugation,small-angle X-ray scattering and electron microscopy[J].J Mol Biol,2007,365(1):187-195.
[28]LI Y,XIE X,SHI X,et al. Vibrio parahaemolyticus,Southern Coastal Region of China,2007-2012[J].Emerg Infect Dis,2014,20(4):685-688.
[29]JOSEPH S,COLWELL R,KAPER J. Vibrio parahaemolyticus and related halophilic Vibrios[J]. Crit Rev Microbiol,1982,10(1):77-124.
[30]LANGP,KAISERS,MYSSINAS,etal.Effectof Vibrioparahaemolyticus haemolysin on human erythrocytes[J].Cell Microbiol,2004,6(4):391-400.
[31]SHIMOHATA T,TAKAHASHI A. Diarrhea induced by infection of Vibrio parahaemolyticus[J].J Med Invest,2010,57(3-4):179-182.
[32]KISHISHITA M,MATSUOKA N,KUMAGAI K,et al. Sequence variation in the thermostable direct hemolysin-related hemolysin(trh)gene of Vibrio parahaemolyticus[J]. Appl Environ Microbiol,1992,58(8):2449-2457.
[33]陈茂义,胡婕,陈婷,等.副溶血性弧菌毒力基因研究进展[J].公共卫生与预防医学,2013,24(3):65-67.
[34]黄彦,晁耐霞,庞毅恒.副溶血性弧菌致病性研究进展[J].岭南急诊医学杂志,2018,23(1):88-90.
[35]SUTHIENKUL O,ISHIBASHI M,IIDA T,et al.Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand[J]. J Infect Dis,1995,172(5):1405-1408.
[36]GOTOH K,KODAMA T,HIYOSHI H,et al. Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants[J]. PLOS ONE,2010,5(10):e13365.
[37]OKADA N,MATSUDA S,MATSUYAMA J,et al. Presence of genes for type III secretion system 2 in Vibrio mimicus strains[J/OL].BMC Microbiol,2010(10):302.[2018-10-29].https://www.ncbi.nlm.nih.gov/pubmed/21110901.DOI:10.1186/1471-2180-10-302.
[2]邢丽萍,周虓.创伤弧菌生物学研究进展[J].中国卫生检验杂志,2011,21(7):1833-1836.
[3]JEONG H,SATCHELL K. Additive function of Vibrio vulnificus MARTX(Vv)and VvhA cytolysins promotes rapid growth and epithelial tissue necrosisduringintestinalinfection[J].PLoSPathog,2012,8(3):e1002581.
[4]叶淑瑶,杨保伟,李凤琴.创伤弧菌致病性及致病机制研究进展[J].中国食品卫生杂志,2018,30(2):213-219.
[5] KWON K,YANG J,RYU D,et al. Vibrio vulnificus cytolysin induces superoxide anion-initiated apoptotic signaling pathway in human ECV304 cells[J]. J Biol Chem,2001,276(50):47518-47523.
[6]李桂军,楼永良.创伤弧菌细胞素致病机制的研究进展[J].国际检验医学杂志,2006(7):633-635.
[7]MIYOSHI S,NARUKAWA H,TOMOCHIKA K,et al. Actions of Vibrio vulnificus metalloprotease on human plasma proteinase-proteinase inhibitor systems:a comparative study of native protease with its derivative modified by polyethylene glycol[J]. Microbiol Immunol,1995,39(12):959-966.
[8]滕勇勇,王琪,吴雷,等.致病性弧菌的生物学特性和致病因子研究进展[J].热带医学杂志,2014,14(10):1396-1399.
[9]DATTA S,CROSA JH. Identification and characterization of a novel outer membrane protein receptor required for hemin utilization in Vibrio vulnificus[J]. Biometals,2012,25(2):275-283.
[10]PAJUELO D,HERNANDEZ-CABANYERO C,SAJUAN E,et al.Iron and Fur in the life cycle of the zoonotic pathogen Vibrio vulnificus[J]. Environ Microbiol,2016,18(11):4005-4022.
[11]KIM I,SHIM J,LEE K,et al. Nonribosomal peptide synthase is responsible for the biosynthesis of siderophore in Vibrio vulnificus MO6-24/O[J]. J Microbiol Biotechnol,2008,18(1):35-42.
[12]HILTON T,ROSCHE T,FROELICH B,et al. Capsular polysaccharide phase variation in Vibrio vulnificus[J]. Appl Environ Microbiol,2006,72(11):6986-6993.
[13]郑晨晖.黄姑鱼源溶藻弧菌的分离鉴定[J].北京农业,2014(27):7-9.
[14]彭喜春,张宁,冉艳红,等.海产品中溶藻弧菌的筛选及其致病因子的研究[J].现代食品科技,2008(4):312-315.
[15]BAFFONE W,CITTERIO B,VITTORIA E,et al. Determination of several potential virulence factors in Vibrio spp isolated from seawater[J/OL].Fod Microbiology,2001(18):479-488.[2018-10-29].https://www.idealibrary.com on.DOI:10.1006/fmic.2001.0441
[16]鄢庆枇,苏永全,王军.溶藻弧菌脂多糖对大黄鱼的毒性与免疫保护性试验[J].台湾海峡,2003,22(2):163-167.
[17]BALEBONA M,ANDREU M,BORDAS M,et al. Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream(Sparus aurata L.)[J].Appl Environ Microbiol,1998,64(11):4269-4275.
[18]苑淑宾,朱爱意.溶藻弧菌对水产动物致病性及其防治的研究进展[J].浙江海洋学院学报(自然科学版),2012,31(3):256-264.
[19]BELAS M,COLWELL R. Adsorption kinetics of laterally and polarly flagellated Vibrio[J]. J Bacteriol,1982,151(3):1568-1580.
[20]BORDAS M,BALEBONA M,ZOEEILLA I,et al. Kinetics of adhesion of selected fish-pathogenic Vibrio strains of skin mucus of gilt-head sea bream(Sparus aurata L.)[J].Appl Environ Microbiol,1996,62(10):3650-3654.
[21]王蓬勃,马悦,刘琴.溶藻弧菌铁载体合成及外膜蛋白表达的研究[J].微生物学报,2006,33(2):48-53.
[22]梅冰,陆翔,王丽娜,等.溶藻弧菌的毒力因子与相关基因的研究进展[J].辽宁农业科学,2015(5):58-60.
[23]毕振强,赵仲堂.霍乱弧菌致病因子及调控基因研究进展[J].中国公共卫生,2005,21(6):754-755.
[24]TAYLOR R,KIRN T,MEEKS M,et al. A Vibrio cholerae classical TcpA amino acid sequence induces protective antibody that binds an area hypothesized to be important for toxin-coregulated pilus structure[J].Infect Immun,2004,72(10):6050-6060.
[25]KARAOLIS D,SOMARA S,MANEVAL D,et al. A bacteriophage encoding a pathogenicity island, a type-IV pilus and a phage receptor in cholera bacteria[J].Nature,1999,399(6734):375-379.
[26]王红.副溶血性弧菌传播流行及分子检测技术概述[J].大众科技,2018,20(4):46-48,53.
[27] HAMADA D,HIGURASHI T,MAYANAGI K,et al. Tetrameric structure of thermostable direct hemolysin from vibrio parahaemolyticus revealed by ultracentrifugation,small-angle X-ray scattering and electron microscopy[J].J Mol Biol,2007,365(1):187-195.
[28]LI Y,XIE X,SHI X,et al. Vibrio parahaemolyticus,Southern Coastal Region of China,2007-2012[J].Emerg Infect Dis,2014,20(4):685-688.
[29]JOSEPH S,COLWELL R,KAPER J. Vibrio parahaemolyticus and related halophilic Vibrios[J]. Crit Rev Microbiol,1982,10(1):77-124.
[30]LANGP,KAISERS,MYSSINAS,etal.Effectof Vibrioparahaemolyticus haemolysin on human erythrocytes[J].Cell Microbiol,2004,6(4):391-400.
[31]SHIMOHATA T,TAKAHASHI A. Diarrhea induced by infection of Vibrio parahaemolyticus[J].J Med Invest,2010,57(3-4):179-182.
[32]KISHISHITA M,MATSUOKA N,KUMAGAI K,et al. Sequence variation in the thermostable direct hemolysin-related hemolysin(trh)gene of Vibrio parahaemolyticus[J]. Appl Environ Microbiol,1992,58(8):2449-2457.
[33]陈茂义,胡婕,陈婷,等.副溶血性弧菌毒力基因研究进展[J].公共卫生与预防医学,2013,24(3):65-67.
[34]黄彦,晁耐霞,庞毅恒.副溶血性弧菌致病性研究进展[J].岭南急诊医学杂志,2018,23(1):88-90.
[35]SUTHIENKUL O,ISHIBASHI M,IIDA T,et al.Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand[J]. J Infect Dis,1995,172(5):1405-1408.
[36]GOTOH K,KODAMA T,HIYOSHI H,et al. Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants[J]. PLOS ONE,2010,5(10):e13365.
[37]OKADA N,MATSUDA S,MATSUYAMA J,et al. Presence of genes for type III secretion system 2 in Vibrio mimicus strains[J/OL].BMC Microbiol,2010(10):302.[2018-10-29].https://www.ncbi.nlm.nih.gov/pubmed/21110901.DOI:10.1186/1471-2180-10-302.
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