四逆散有效组分拮抗空瓶刺激所致大鼠慢性情绪应激的作用
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摘要
目的:探讨四逆散有效组分拮抗慢性情绪应激大鼠的作用。方法:利用连续21 d空瓶刺激所致大鼠慢性情绪应激模型,观察四逆散有效组分(450 mg/kg/day,连续21 d)对模型大鼠的体重、行为学、血清皮质酮含量及大鼠海马组织病变的影响。结果:四逆散有效组分能够有效拮抗空瓶刺激诱导的大鼠慢性情绪应激反应,表现为大鼠体重明显升高、攻击和探究行为减少、修饰行为增加、皮质酮含量降低、改善情绪应激导致的海马损伤。结论:四逆散有效组分可能是通过抑制皮质酮的含量升高,减少海马损伤而发挥其拮抗慢性情绪应激的作用。
Objective: To explore the reversal effects of Sinisan active ingredients on chronic emotional stress in rats.Methods: The rats received empty bottle stimulation for 21 days in order to make chronic emotional stress. The modeled rats received Sinisan active ingredients treatment( 450 mg/kg/day,i. g.)or saline in the same volume for 21 days,respectively. The changing of body weight,behavioristics,concentration of serum corticosterone and central hippocampus neuronal tissue in model rats were measured as the main indexes.Results: Sinisan active ingredients had protective effect on rat's chronic emotional stress induced by empty bottles,and the effect was characterized by enhancement of the body weight gain,reduction of aggressive and exploratory behaviors,increase of grooming behavior,reduced level of serum corticosterone, and development of hippocampus injuries.Conclusion: The results suggested that inhibition of the elevated corticosterone levels and reduction of the area of hippocampus injuries may play important roles in the reversal effects of active ingredients of Sinisan on chronic emotional stress.
Objective: To explore the reversal effects of Sinisan active ingredients on chronic emotional stress in rats.Methods: The rats received empty bottle stimulation for 21 days in order to make chronic emotional stress. The modeled rats received Sinisan active ingredients treatment( 450 mg/kg/day,i. g.)or saline in the same volume for 21 days,respectively. The changing of body weight,behavioristics,concentration of serum corticosterone and central hippocampus neuronal tissue in model rats were measured as the main indexes.Results: Sinisan active ingredients had protective effect on rat's chronic emotional stress induced by empty bottles,and the effect was characterized by enhancement of the body weight gain,reduction of aggressive and exploratory behaviors,increase of grooming behavior,reduced level of serum corticosterone, and development of hippocampus injuries.Conclusion: The results suggested that inhibition of the elevated corticosterone levels and reduction of the area of hippocampus injuries may play important roles in the reversal effects of active ingredients of Sinisan on chronic emotional stress.
引文
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[14]黄文英,周萍,杨晓艇,等.构建影响免疫功能的慢性心理应激动物模型:不同应激源刺激差异比较[J].中国组织工程研究与临床康复,2007,51:10308-10311.
[15]邵枫,林文娟,陈极寰.慢性情绪应激对大鼠行为的影响及变化趋势[J].中国行为医学科学,2003(1):5-6+17.
[16]刘莉,凤林谱,刘新民.情绪和生理应激对大鼠行为及血粘度的影响[J].皖南医学院学报,2010(3):176-178.
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[2]闫兵,杨卓欣,皮敏,等.四逆散病机及其临床应用的研究进展[J].中国当代医药,2017,24(33):17-20.
[3]贾子尧,林瑞超,马志强,等.四逆散药理作用和临床应用文献研究[J].辽宁中医药大学学报,2017(6):159-162.
[4]蒲应炎,潘晓微,潘奔前.四逆散加味配合耳穴治疗不寐83例疗效观察[J].中医药临床杂志,2016(8):1179-1180.
[5]李廷利,黄玉新,都晓伟.中药复方四逆散水煎物HPLC指纹图谱研究[J].世界科学技术,2005,S1:48-52.
[6]李越峰,严兴科,李廷利,等.四逆散冻干粉化学成分变化的研究[J].中草药,2010(6):854-859.
[7]李越峰,李廷利,严兴科.四逆散冻干粉及其拆方镇静催眠作用的等效性研究[J].中国临床药理学杂志,2014(8):719-720.
[8]黄莉莉,徐瑞鑫,卞宏生,等.基于5-HT能神经系统的四逆散冻干粉改善睡眠的作用机制研究[J].哈尔滨医科大学学报,2011(3):214-217.
[9]陈霞,杨世杰,陈立,等.下丘脑外侧区注射人参单体皂甙Rb1的抗应激作用及其机理[J].中国药学杂志,1994,29(7):401-404.
[10]居同法,赵雪俭.人参皂甙抗鼠应激作用研究进展[J].人参研究,1999(3):6-9.
[11]金鑫.山药多糖对小鼠的抗应激作用研究[J].河南农业科学,2015,44(1):143-145.
[12]郭宏伟.中药拮抗慢性应激药理研究的现状[J].暨南大学学报(自然科学与医学版),2017,38(1):86-92.
[13]林文娟,王玮雯,邵枫.慢性情绪应激对大鼠行为、神经内分泌和免疫反应的影响:一个新的情绪应激模型[J].科学通报,2003(9):926-929.
[14]黄文英,周萍,杨晓艇,等.构建影响免疫功能的慢性心理应激动物模型:不同应激源刺激差异比较[J].中国组织工程研究与临床康复,2007,51:10308-10311.
[15]邵枫,林文娟,陈极寰.慢性情绪应激对大鼠行为的影响及变化趋势[J].中国行为医学科学,2003(1):5-6+17.
[16]刘莉,凤林谱,刘新民.情绪和生理应激对大鼠行为及血粘度的影响[J].皖南医学院学报,2010(3):176-178.
[17]赵虎,徐小虎,杨德森.皮质类固醇受体系统对中枢应激反应的调节[J].国外医学.精神病学分册,2003(1):55-59.
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