紫花高乌头药材的质量标准研究

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英文篇名：Study on Quality Standard for Aconitum excelsum Reichb 作者：史巧霞 ; 朱应怀 ; 靳子明 英文作者：SHI Qiaoxia;ZHU Yinghuai;JIN Ziming;Gansu University of Chinese Medicine,Preparation Center;Provincial-Level Key Laboratory for Tibet Herbal Chemicals and Quality Research inGansu Colleges and Universities; 关键词：紫花高乌头 ; 质量标准 ; 薄层色谱法 ; 高效液相色谱法 ; 指纹图谱 ; 高乌甲素 ; 冉乌头碱 英文关键词：Aconitum excelsum Reichb;;quality standard;;TLC;;HPLC;;fingerprint;;lappacontine;;ranaconitine 中文刊名：ZYXY 英文刊名：Traditional Chinese Drug Research & Clinical Pharmacology 机构：甘肃中医药大学附属医院制剂中心;甘肃省中藏药化学与质量研究省级重点实验室; 出版日期：2019-03-25 出版单位：中药新药与临床药理 年：2019 期：v.30;No.156 基金：国家自然科学基金(81460611);; 甘肃省中医药管理局科研课题(GZK-2013-54) 语种：中文; 页：ZYXY201903017 页数：5 CN：03 ISSN：44-1308/R 分类号：90-94

摘要

目的建立紫花高乌头药材的质量标准,提高其质量控制水平。方法采用薄层色谱(TLC)对不同产地紫花高乌头药材进行定性鉴别;按《中国药典》方法测定其水分、灰分与浸出物;采用高效液相色谱-二极管阵列检测(HPLC-DAD)技术建立不同产地紫花高乌头药材指纹图谱并对其有效成分进行含量测定。结果不同产地紫花高乌头药材TLC斑点清晰,分离度好;暂定其药材水分、总灰分及酸不溶性灰分分别不得过10.0%、11.0%、4.5%,醇溶性浸出物应不少于24.0%;建立了紫花高乌头药材指纹图谱,标定共有峰14个,紫花高乌头药材中高乌甲素与冉乌头碱的含量应不少于0.126%、0.018%。结论该研究所建立的方法准确可靠、重复性好、特征性强,可用于紫花高乌头药材的质量评价。
        Objective To improve the quality standard of Aconitum excelsum Reichb for its quality control. Methods The thin layer chromatography(TLC) were used for qualitative identification of Aconitum excelsum Reichb from different areas;and the pharmacopeia methods were used for the tests of moisture,ash and extractives. At the same time,HPLC-DAD method was developed to establish fingerprints for Aconitum excelsum Reichb from different areas and determination of the contents. Results The TLC showed the spots developed clearly and the separation was good. The moisture,total ash and acid insoluble ash of Aconitum excelsum Reichb should not be more than 10.0%,11.0%,4.5%,the alcohol soluble extractives should not be less than 24.0%,respectively. The common profile for Aconitum excelsum Reichb fingerprint was established, and 14 common fingerprint peaks were identified. The lappacontine and ranaconitine of Aconitum excelsum Reichb should not be less than 0.126% and 0.018%,respectively. Conclusion The methods developed were accurate,reproducible with high specificity,which can be used for quality control of Aconitum excelsum Reichb in the future.

引文

[1]桂香,包海泉.蒙药紫花高乌头的研究进展[J].中国民族医药杂志,2012,18(1):73-75.
    [2]朱亚民.内蒙古植物药志[M].呼和浩特:内蒙古人民出版社,1989:369.
    [3]杨玉梅,张慧灵,刘和莉,等.紫花高乌头总碱对大鼠实验性心律失常的影响[J].包头医学院学报,2000,16(2):87-88.
    [4]韩瑞兰,盘乐鸯,王红红,等.蒙药紫花高乌头醇提物镇咳祛痰作用研究[J].北方药学,2010,7(3):19-20.
    [5]呼格吉勒图,陶高.紫花高乌头治疗慢支225例体会[J].内蒙古医学杂志,1992,12(2):59-60.
    [6]SAYED H M,DESAI H K,ROSS S A,et al.New diterpenoid alkaloids from the roots of Aconitum septentrionale:isolation by an ion exchange method[J].J Nat Prod,1992,55(11):1595-1606.
    [7]张树祥,贾世山.蒙药紫花高乌头根中新二萜生物碱的分离和鉴定[J].药学学报,1999,34(10):762-766.
    [8]张立军,戴海蓉,李芸,等.高乌头炮制品质量标准研究[J].中华中医药杂志,2018,33(02):675-678.
    [9]国家药典委员会.中华人民共和国药典(四部)[M].北京:化学工业出版社,2015:2101-2301.
    [10]谢凡,吴迎春,王峥涛,等.葎草药材的质量标准研究[J].中国中药杂志,2014,39(20):3986-3990.
    [11]周礼仕,王毓杰,张艺,等.藏药康定乌头质量标准研究[J].中药材,2014,37(6):977-980.
    [12]常福厚,韩瑞兰,齐君,等.高效液相色谱法测定紫花高乌头中乌头碱的含量[J].时珍国医国药,2009,20(5):1164-1165.
    [13]任文栓,马佳,常福厚.紫外-可见分光光度法测定紫花高乌头中乌头碱的含量[J].内蒙古医学杂志,2010,42(2):136-139.