美国流苏嫩枝扦插及生根内源激素含量的变化
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摘要
剪取美国流苏嫩枝作为插穗,研究外源激素、插穗部位、留叶类型等因素对扦插生根的影响,寻求更优的生根处理方式;测定生根进程中内源激素的含量,揭示其与生根的内在关系。结果表明,外源激素3因素对生根率的影响程度为种类>处理时间>浓度,均达到显著差异水平,500mg/L的IBA浸泡60min得到的生根率最高,为56.67%;枝条中部作为插穗时生根率显著高于基部和顶部,为55.00%;插穗保留2片1/2叶时生根率最高,为53.33%,无叶处理的各项指标最差;经IBA处理插穗IAA含量在愈伤组织形成期升高,不定根形成期下降,对照处于不断下降趋势;ABA含量表现为先上升后下降的趋势;GA3、ZR含量在愈伤组织的形成期较高,不定根形成期较低;IBA处理提升了IAA、GA3、ZR含量,降低了ABA含量,有利于插穗生根。
Shoots of Chionanthus virginicus were used as materials to conduct cutting experiment.Effects of exogenous hormones,cutting positions and leaf blade numbers reserved on rooting were examined to seek better rooting treatment.The contents of endogenous hormones during rooting were measured to reveal the intrinsic relationship between the hormones and rooting.The results showed that the influences of three factors related to exogenous hormones on rooting rate was hormone type>treatment time>concentration.The differences among the factors were significantly different.The highest rooting rate(56.67%)was found in the treatment of 500 mg/L IBA soaked for 60 min.The rooting rate of using middle part of shoots as cuttings was 55.00%,significantly higher than those of using shoot base and tip.The highest rooting rate(53.33%)achieved when 2 or 1/2 leaf blades were reserved in the cuttings,and all the indexes without leaf treatment were the worst.The IAA content of the IBA-treated cuttings increased in callus formation period,and decreased in the formation period of adventitious roots,and decreased all the time in the control.The ABA content showed a trend of rising first and then decreasing.The contents of GA3 and ZR were higher in the formation of callus and lower in the formation period of adentitious roots.IBA treatment promoted the contents of IAA,GA3 and ZR in cuttings,reduced the content of ABA,and was conducive to rooting of cuttings.
Shoots of Chionanthus virginicus were used as materials to conduct cutting experiment.Effects of exogenous hormones,cutting positions and leaf blade numbers reserved on rooting were examined to seek better rooting treatment.The contents of endogenous hormones during rooting were measured to reveal the intrinsic relationship between the hormones and rooting.The results showed that the influences of three factors related to exogenous hormones on rooting rate was hormone type>treatment time>concentration.The differences among the factors were significantly different.The highest rooting rate(56.67%)was found in the treatment of 500 mg/L IBA soaked for 60 min.The rooting rate of using middle part of shoots as cuttings was 55.00%,significantly higher than those of using shoot base and tip.The highest rooting rate(53.33%)achieved when 2 or 1/2 leaf blades were reserved in the cuttings,and all the indexes without leaf treatment were the worst.The IAA content of the IBA-treated cuttings increased in callus formation period,and decreased in the formation period of adventitious roots,and decreased all the time in the control.The ABA content showed a trend of rising first and then decreasing.The contents of GA3 and ZR were higher in the formation of callus and lower in the formation period of adentitious roots.IBA treatment promoted the contents of IAA,GA3 and ZR in cuttings,reduced the content of ABA,and was conducive to rooting of cuttings.
引文
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[14]CONTESSA C,VALENTINI N,BOTTA R.Decreasing the concentration of IBA or combination with ethylene inhibitors improve bud retention in semi-hardwood cuttings of hazelnut cultivar‘Tonda Gentile delle Langhe’[J].Scientia Horticulturae,2011(131):103-106.
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[17]徐世彦,梁建军,梁彬,等.不同处理对吉塞拉系樱桃砧木扦插效果的影响[J].西北农业学报,2017,26(1):54-60.XU S Y,LIANG J J,LIANG B,et al.Factors affecting cutting propagation of two Gisela sweet cherry rootstocks[J].Acta Agriculturae Boreali-occidentalis Sinica,2017,26(1):54-60.(in Chinese)
[18]梁有旺,彭方仁,何雅萍.常绿白蜡扦插繁殖技术[J].林业科技开发,2010,24(1):110-112.LIANG Y W,PENG F R,HE Y P.Cutting propagation of Fraxinus griffithii[J].Journal of Forestry Engineering,2010,24(1):110-112.(in Chinese)
[19]VAKOUFTSIS G,SYROS T,KOSTAS S,et al.Effect of IBA,time of cutting collection,type of cuttings and rooting substrate on vegetative propagation in Cupressus macrocarpa‘goldcrest’[J].Propagation of Ornamental Plants,2009,9(2):65-70.
[20]郭有燕,余宏远,吕彪,等.扦插生根影响因素重要性排序的研究[J].西北林学院学报,2013,28(4):103-105.GUO Y Y,YU H Y,LYU B,et al.Importance order of the factors affecting cutting rooting[J].Journal of Northwest Forestry University,2013,28(4):103-105.(in Chinese)
[21]OPUNI-FRIMPONG E,KARNOSKY D F,STORER A J,et al.Key roles of leaves,stockplant age,and auxin concentration in vegetative propagation of two African mahoganies:Khaya anthotheca Welw.and Khaya ivorensis A.Chev[J].New Forests,2008,36(2):115-123.
[22]唐玲,王艳芳,李荣英,等.插穗规格与遮荫度对肾茶扦插苗质量的影响[J].中药材,2017,40(2):281-283.
[23]KELEN M,OZKAN G.Relationships between rooting ability and changes of endogenous IAA and ABA during the rooting of hardwood cuttings of some grapevine rootstocks[J].European Journal of Horticultural Science,2003,68(1):8-13.
[24]李朝婵,赵云龙,张冬林,等.长蕊杜鹃扦插内源激素变化及解剖结构观察[J].林业科学研究,2012,25(3):360-365.LI C C,ZHAO Y L,ZHANG D L,et al.Changes of endogenous hormones and anatomical structure of Rhododendron stamineum during cutting propagation[J].Forest Research,2012,25(3):360-365.(in Chinese)
[25]马振华,王吉斌,周贵平,等.四倍体刺槐嫩枝扦插生根过程中内源激素的变化[J].西北林学院学报,2013,28(5):18-23.MA Z H,WANG J B,ZHOU G P,et al.Studied on changes hormone in the periods of making roots in the softwood of Robinia pseudoacacia[J].Journal of Northwest Forestry University,2013,28(5):18-23.(in Chinese)
[26]黄志玲,郝海坤,曹艳云,等.红锥扦插生根过程中内源激素的变化[J].中南林业科技大学学报,2015,35(2):22-25.HUANG Z L,HAO H K,CAO Y Y,et al.Changes of endogenous hormones in Castanopsis hystrix cuttings[J].Journal of Central South University of Forestry&Technology,2015,35(2):22-25.(in Chinese)
[27]吴文浩,曹凡,刘壮壮,等.NAA对薄壳山核桃扦插生根过程中内源激素含量变化的影响[J].南京林业大学学报:自然科学版,2016,40(5):191-196.WU W H,CAO F,LIU Z Z,et al.Effects of NAA treatment on the endogenous hormone changes in cuttings of Carya illinoinensis during rooting[J].Journal of Nanjing Forestry University:Natural Sciences Edition,2016,40(5):191-196.(in Chinese)
[28]董胜君,刘明国,戴菲,等.山杏嫩枝扦插生根过程中插穗内源激素含量的变化[J].经济林研究,2013,31(4):108-114.DONG S J,LIU M G,DAI F,et al.Variation of endogenous hormone contents in softwood cuttings of Armeniaca sibirica during adventitious root formation[J].Nonwood Forest Research,2013,31(4):108-114.(in Chinese)
[29]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.OUYANG F Q,FU G Z,WANG J H,et al.Qualitative analysis of endogenesis hormone and polyphenol during rooting of cuttings in norway spruce(Picea abies)[J].Scientia Silvae Sinicae,2015,51(3):155-162.(in Chinese)
[30]杨雪萌,房伟民,陈发棣,等.两个菊花品种扦插生根过程及其插穗碳氮营养和内源激素的变化[J].南京农业大学学报,2010,33(4):19-24.YANG X M,FANG W M,CHEN F D,et al.Cuttings rooting process and changes in carbohydrate,nitrogen and endogenous hormone levels during the rooting of two chrysanthemum cultivars[J].Journal of Nanjing Agricultural University,2010,33(4):19-24.(in Chinese)
[31]王艳晶,彭祚登.不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J].西北林学院学报,2017,32(5):109-114.WANG Y J,PENG Z D.Effects of different rooting accelerators on the changes of endogenous hormones in Sophora japonica during softwood-cutting rooting process[J].Journal of Northwest Forestry University,2017,32(5):109-114.(in Chinese)
[32]何崇单,姚锐,黄瑞春,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.HE C D,YAO R,HUANG R C,et al.Effects of K-IBA treatments on adventitious rooting and endogenous hormones contents of shoot cuttings of Thuja occidentals L.[J].Journal of Central South University of Forestry&Technology,2017,37(10):7-12.(in Chinese)
[33]刘昊,宋晓波,周乃富,等.吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响[J].浙江农林大学学报,2017,34(6):1038-1043.LIU H,SONG X B,ZHOU N F,et al.Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings[J].Journal of Zhejiang A&F University,2017,34(6):1038-1043.(in Chinese)
[2]BOYER L,BAGHDIKIAN B,BUN S S,et al.Chionanthus virginica L.:phytochemical analysis and quality control of herbal drug and herbal preparations[J].Nat Prod Commun,2011,6(6):753-800.
[3]PENMAN K G,BONE K M,LEHMANN R P.Fringe tree(Chionanthus virginica L.)stem bark offers a sustainable alternative to root bark[J].Australian Journal of Medical Herbalism,2008,20(3):107.
[4]GLIN,ELIAS R,GEPDIREMEN A,et al.Antioxidant secoiridoids from fringe tree(Chionanthus virginicus L.)[J].Wood Science and Technology,2009,43(34):195-212.
[5]REDCAY S,FRETT J J.Germination of doubly dormant Chionanthus virginicus seeds[J].Hort Science,1990,25(6):627.
[6]孟玲玲,张鸽香.美国流苏种子休眠原因探析[J].广东农业科学,2015,42(6):35-39.MENG L L,ZHANG G X.Causes of dormancy of Chionanthus virginicus seed[J].Guangdong Agricultural Sciences,2015,42(6):35-39.(in Chinese)
[7]张鸽香,孟玲玲.美国流苏种子酸蚀处理后种子结构的变化[J].江苏农业科学,2015,43(10):245-247.
[8]李继华.扦插的原理与应用[M].上海:上海科学技术出版社,1987:16-22.
[9]金建邦,祝遵凌,林庆梅.欧洲鹅耳枥扦插生根及解剖特性[J].西北农林科技大学学报:自然科学版,2015,43(2):92-98.JIN J B,ZHU Z L,LIN Q M.Rooting characteristics and anatomical structure of Carpinus betulus cuttings[J].Journal of Northwest A&F Univcrsity:Nat.Sci.Ed.,2015,43(2):92-98.(in Chinese)
[10]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:231-234.
[11]时军霞.不同浓度IBA和NAA处理流苏树茎段对扦插生根的影响[J].山东农业科学,2011(9):55-56.
[12]魏巍,张鸽香.流苏树嫩枝扦插及生根营养物质的动态变化[J].河南农业科学,2015,44(10):127-131.
[13]宋越冬.不同激素处理对流苏扦插繁殖的影响[J].林业科技,2008,33(3):13-14.
[14]CONTESSA C,VALENTINI N,BOTTA R.Decreasing the concentration of IBA or combination with ethylene inhibitors improve bud retention in semi-hardwood cuttings of hazelnut cultivar‘Tonda Gentile delle Langhe’[J].Scientia Horticulturae,2011(131):103-106.
[15]HOSSAIN M A,ISLAM M A,HOSSAIN M M.Rooting ability of cuttings of Swietenia macrophylla King and Chukrasia velutina wight et arn as influenced by exogenous hormone[J].International Journal of Agriculture&Biology,2003(3):560-564.
[16]王业社,杨贤均,陈立军,等.藤本植物千里光的无性扦插繁殖技术[J].草业科学,2014,31(6):1120-1125.
[17]徐世彦,梁建军,梁彬,等.不同处理对吉塞拉系樱桃砧木扦插效果的影响[J].西北农业学报,2017,26(1):54-60.XU S Y,LIANG J J,LIANG B,et al.Factors affecting cutting propagation of two Gisela sweet cherry rootstocks[J].Acta Agriculturae Boreali-occidentalis Sinica,2017,26(1):54-60.(in Chinese)
[18]梁有旺,彭方仁,何雅萍.常绿白蜡扦插繁殖技术[J].林业科技开发,2010,24(1):110-112.LIANG Y W,PENG F R,HE Y P.Cutting propagation of Fraxinus griffithii[J].Journal of Forestry Engineering,2010,24(1):110-112.(in Chinese)
[19]VAKOUFTSIS G,SYROS T,KOSTAS S,et al.Effect of IBA,time of cutting collection,type of cuttings and rooting substrate on vegetative propagation in Cupressus macrocarpa‘goldcrest’[J].Propagation of Ornamental Plants,2009,9(2):65-70.
[20]郭有燕,余宏远,吕彪,等.扦插生根影响因素重要性排序的研究[J].西北林学院学报,2013,28(4):103-105.GUO Y Y,YU H Y,LYU B,et al.Importance order of the factors affecting cutting rooting[J].Journal of Northwest Forestry University,2013,28(4):103-105.(in Chinese)
[21]OPUNI-FRIMPONG E,KARNOSKY D F,STORER A J,et al.Key roles of leaves,stockplant age,and auxin concentration in vegetative propagation of two African mahoganies:Khaya anthotheca Welw.and Khaya ivorensis A.Chev[J].New Forests,2008,36(2):115-123.
[22]唐玲,王艳芳,李荣英,等.插穗规格与遮荫度对肾茶扦插苗质量的影响[J].中药材,2017,40(2):281-283.
[23]KELEN M,OZKAN G.Relationships between rooting ability and changes of endogenous IAA and ABA during the rooting of hardwood cuttings of some grapevine rootstocks[J].European Journal of Horticultural Science,2003,68(1):8-13.
[24]李朝婵,赵云龙,张冬林,等.长蕊杜鹃扦插内源激素变化及解剖结构观察[J].林业科学研究,2012,25(3):360-365.LI C C,ZHAO Y L,ZHANG D L,et al.Changes of endogenous hormones and anatomical structure of Rhododendron stamineum during cutting propagation[J].Forest Research,2012,25(3):360-365.(in Chinese)
[25]马振华,王吉斌,周贵平,等.四倍体刺槐嫩枝扦插生根过程中内源激素的变化[J].西北林学院学报,2013,28(5):18-23.MA Z H,WANG J B,ZHOU G P,et al.Studied on changes hormone in the periods of making roots in the softwood of Robinia pseudoacacia[J].Journal of Northwest Forestry University,2013,28(5):18-23.(in Chinese)
[26]黄志玲,郝海坤,曹艳云,等.红锥扦插生根过程中内源激素的变化[J].中南林业科技大学学报,2015,35(2):22-25.HUANG Z L,HAO H K,CAO Y Y,et al.Changes of endogenous hormones in Castanopsis hystrix cuttings[J].Journal of Central South University of Forestry&Technology,2015,35(2):22-25.(in Chinese)
[27]吴文浩,曹凡,刘壮壮,等.NAA对薄壳山核桃扦插生根过程中内源激素含量变化的影响[J].南京林业大学学报:自然科学版,2016,40(5):191-196.WU W H,CAO F,LIU Z Z,et al.Effects of NAA treatment on the endogenous hormone changes in cuttings of Carya illinoinensis during rooting[J].Journal of Nanjing Forestry University:Natural Sciences Edition,2016,40(5):191-196.(in Chinese)
[28]董胜君,刘明国,戴菲,等.山杏嫩枝扦插生根过程中插穗内源激素含量的变化[J].经济林研究,2013,31(4):108-114.DONG S J,LIU M G,DAI F,et al.Variation of endogenous hormone contents in softwood cuttings of Armeniaca sibirica during adventitious root formation[J].Nonwood Forest Research,2013,31(4):108-114.(in Chinese)
[29]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.OUYANG F Q,FU G Z,WANG J H,et al.Qualitative analysis of endogenesis hormone and polyphenol during rooting of cuttings in norway spruce(Picea abies)[J].Scientia Silvae Sinicae,2015,51(3):155-162.(in Chinese)
[30]杨雪萌,房伟民,陈发棣,等.两个菊花品种扦插生根过程及其插穗碳氮营养和内源激素的变化[J].南京农业大学学报,2010,33(4):19-24.YANG X M,FANG W M,CHEN F D,et al.Cuttings rooting process and changes in carbohydrate,nitrogen and endogenous hormone levels during the rooting of two chrysanthemum cultivars[J].Journal of Nanjing Agricultural University,2010,33(4):19-24.(in Chinese)
[31]王艳晶,彭祚登.不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J].西北林学院学报,2017,32(5):109-114.WANG Y J,PENG Z D.Effects of different rooting accelerators on the changes of endogenous hormones in Sophora japonica during softwood-cutting rooting process[J].Journal of Northwest Forestry University,2017,32(5):109-114.(in Chinese)
[32]何崇单,姚锐,黄瑞春,等.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12.HE C D,YAO R,HUANG R C,et al.Effects of K-IBA treatments on adventitious rooting and endogenous hormones contents of shoot cuttings of Thuja occidentals L.[J].Journal of Central South University of Forestry&Technology,2017,37(10):7-12.(in Chinese)
[33]刘昊,宋晓波,周乃富,等.吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响[J].浙江农林大学学报,2017,34(6):1038-1043.LIU H,SONG X B,ZHOU N F,et al.Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings[J].Journal of Zhejiang A&F University,2017,34(6):1038-1043.(in Chinese)
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