响应面法优化栀子中绿原酸的提取工艺

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英文篇名：Optimization of Extraction Process of Chlorogenic Acid from Gardenia jasminoides Ellis by Response Surface Methodology 作者：易梦媛 ; 邓爱华 ; 刘凤英 ; 王云 ; 谢鹏 ; 卢俊明 ; 王志 英文作者：YI Mengyuan;DENG Aihua;LIU Fenying;WANG Yun;XIE Peng;LU Junming;WANG Zhi;College of Life and Environment Science,Hu'nan University of Arts and Science;College of Furong,Hu'nan University of Arts and Science;Lixian Funong Professional Cooperatives of Chinese Herbal Medicine; 关键词：超声波辅助 ; 栀子 ; 绿原酸 ; 响应面法 ; 优化 英文关键词：ultrasound assisted;;Gardenia jasminoides Ellis;;chlorogenic acid;;response surface methodology;;optimization 中文刊名：NCPJ 英文刊名：Farm Products Processing 机构：湖南文理学院生命与环境科学学院;湖南文理学院芙蓉学院;澧县扶农中药材农民专业合作社; 出版日期：2019-03-15 出版单位：农产品加工 年：2019 期：No.475 基金：中央引导地方科技发展专项资金项目“万亩药桅子标准化种植及精深加工示范基地”(2017XF5031);; 湖南省教育厅重点项目(17A154);; 湖南省自然科学基金项目(2017JJ4043) 语种：中文; 页：NCPJ201905009 页数：4 CN：05 ISSN：14-1310/S 分类号：32-35

摘要

采用超声波辅助甲醇提取栀子中的绿原酸,考查超声功率、超声时间、甲醇体积分数、料液比、超声温度对栀子中绿原酸提取效果的影响。在单因素试验的基础上选取试验因素与水平,根据中心组合试验设计,采用三因素三水平响应面分析方法,拟合试验因素与响应值的多元二次方程,对各个因素的显著性及交互作用进行分析,运用Design Expert 8.0.6 Trial优化试验条件。得到最佳工艺参数为超声功率72.87 W,超声时间18.46 min,甲醇体积分数60.54%。在此条件下,绿原酸提取率为5.68%。
        The ultrasonic assisted methanol extraction of chlorogenic acid in Gardenia,the effects of ultrasonic power,ultrasonic time,methanol concentration,solid-liquid ratio,ultrasonic temperature on the extraction effect. The effect of selected test factors and levels on the basis of single factor experiment,according to the central composite experimental design,3 factors and 3 levels of response surface analysis multivariate quadratic equation,fitting the experimental factors and response value,was analyzed and the interaction of various factors,the optimum process parameters obtained by ultrasonic extraction under the condition of Design Expert 8.0.6 Trial optimization,ultrasonic power 72.87%,ultrasonic time 18.46 min,the concentration of methanol was 60.54%. Under this condition,the extraction rate of chlorogenic acid was 5.68%.

引文

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