响应面优化超声辅助提取林下参中无机砷

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英文篇名：Optimization of Ultrasonic-assisted Extraction of Inorganic Arsenic in Wild Ginseng by Response Surface Methodology 作者：曾晓丹 ; 胡冉 ; 柳彩云 ; 朱琳 英文作者：Zeng Xiaodan;Hu Ran;Liu Caiyun;Zhu Lin;Analysis and Test Center, Jilin Institute of Chemical Technology;College of Chemical Engineering, East China University of Science and Technology;School of Resources and Environment, University of Jinan;School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology; 关键词：林下参 ; 无机砷 ; 响应面法 ; 超声提取 英文关键词：Wild ginseng;;Inorganic arsenic;;Response surface methodology;;Ultrasonic assistant extraction 中文刊名：FZZW 英文刊名：Molecular Plant Breeding 机构：吉林化工学院分析测试中心;华东理工大学化工学院;济南大学资源与环境学院;吉林化工学院化学与制药工程学院; 出版日期：2019-03-14 出版单位：分子植物育种 年：2019 期：v.17 基金：国家自然基金(21607053);; 吉林市科技局(20166023)共同资助 语种：中文; 页：FZZW201905040 页数：5 CN：05 ISSN：46-1068/S 分类号：305-309

摘要

为了优化林下参中无机砷的超声提取条件,本研究以林下参为原料,无机砷的含量为响应值,筛选提取浓度、提取时间以及提取温度作响应面分析,采用三因素三水平的Box-Behnken试验确定无机砷提取条件,结合原子荧光光谱法测定无机砷的含量。经响应面分析法优化的最佳提取条件为参数为HCl浓度7.1 mol/L,提取温度为42.0℃,提取时间是34.9 min。理论含量为127.65 ng/g,但实测为119.06 ng/g,相对误差为6.73%。证明该模型有效、可靠。与传统方法相比时间短、温度低,具有方便、快捷等优点。本研究结果为人参的加工利用及相关研究提供了参考依据。
        In order to optimize the ultrasonic-assisted extraction process conditions of inorganic arsenic in wild ginseng, three factors, including extraction time, extraction concentration and extraction temperature, were selected to be analyzed by surface methodology using the wild ginseng as a raw materials and the content of inorganic arsenic as the response value in this study. Also, a three-variable and three-level Box-Behnken experimental designs were used to determine the extraction conditions of inorganic arsenic in wild ginseng, and the content of inorganic arsenic was determined by atom fluorescence spectroscopy. After response surface analysis, the optimum extraction conditions were as follows: the HCl concentration was 7.1 mol/L, extraction temperature was42.0℃, and extraction time was 34.9 min. The content of inorganic arsenic in theory and actual extraction process were 127.65 ng/g and 119.06 ng/g, respectively, with a relative error of 6.73%, which indicated that the model was valid and reliable. Compared with the traditional method, this method had the advantages of short time, low temperature, convenience, and rapid extraction process. The results of study could provide reference for the processing and related research of ginseng.

引文

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