中国板栗9个结果母枝相关表型性状遗传多样性研究
|
摘要
以中国10个省份(群体)的164份板栗种质为材料(种植在立地条件相同的同一地块,连续3年采用相同的修剪技术和标准修剪),对母枝结果枝数、雄花枝数、纤弱枝数、结果枝长度、果前梢长度、结果枝节间长度、结果枝粗度、结果枝节位数和结果枝结苞数等9个表型性状量化赋值后进行系统分组设计、方差分析和聚类分析。结果显示:结果母枝的9个表型性状值在群体间和群体内差异均达极显著水平,说明这些性状在群体间和群体内均存在丰富变异;9个表型性状平均变异系数为19.4%～64.0%,其中母枝上雄花枝数、纤弱枝数和果枝结苞数的变异系数> 40%,说明这3个性状改良潜力较大;9个表型性状在群体间表型分化系数为65.9%,群体内为34.1%,说明这些表型性状的变异主要来源于群体间;通过系统聚类,依据表型差异可将10个群体划分为4大类群,基本按地理距离而聚类。
For further enriching genetic diversity information of phenotypic traits on Chinese chestnut germplasm resources,9 phenotypic traits related to biennial bearing branch of 164 Chinese chestnut germplasms from 10 provinces(as 10 populations)were designed with hierarchical classification and then were done with analysis of variance and systematic cluster analysis. The results showed that differences among and within populations of 9 phenotypic traits value were highly significant,indicating a wide range of variation existed in these two levels. The average variation coefficient of the 9 phenotypic traits was 19.4%–64.0%,especially,variation coefficient concerning 3 of the 9 traits including the average male flower shoot number/biennial bearing branch,the delicate shoot number/biennial bearing branch and the average bur number/branch,reached > 40%,which suggested that the germplams had a great potential of genetic breeding in the 3 phenotypic traits. The average of phenotypic differentiation coefficient among populations and within populations were 65.9% and 34.1%,respectively,illustrating that the variationamong populations was the leading source of trait variation. The systematic cluster analysis showed that 10 populations can be divided into 4 groups based on the variation of phenotypic traits,which indicated that the phenotypic traits of Chinese chestnut populations were basically clustered according to geographical distance.
For further enriching genetic diversity information of phenotypic traits on Chinese chestnut germplasm resources,9 phenotypic traits related to biennial bearing branch of 164 Chinese chestnut germplasms from 10 provinces(as 10 populations)were designed with hierarchical classification and then were done with analysis of variance and systematic cluster analysis. The results showed that differences among and within populations of 9 phenotypic traits value were highly significant,indicating a wide range of variation existed in these two levels. The average variation coefficient of the 9 phenotypic traits was 19.4%–64.0%,especially,variation coefficient concerning 3 of the 9 traits including the average male flower shoot number/biennial bearing branch,the delicate shoot number/biennial bearing branch and the average bur number/branch,reached > 40%,which suggested that the germplams had a great potential of genetic breeding in the 3 phenotypic traits. The average of phenotypic differentiation coefficient among populations and within populations were 65.9% and 34.1%,respectively,illustrating that the variationamong populations was the leading source of trait variation. The systematic cluster analysis showed that 10 populations can be divided into 4 groups based on the variation of phenotypic traits,which indicated that the phenotypic traits of Chinese chestnut populations were basically clustered according to geographical distance.
引文
Campbell Diane R.2009.Using phenotypic manipulations to study multivariate selection of floral trait associations.Annals of Botany,103(9):1557-1566.
García-Verdugo C,Fay M F,Granado-Yela C,de Casas R Rubio,Balaguer L,Besnard G,Vargas P.2009.Genetic diversity and differentiation processes in the ploidy series of Olea europaea L.:a multiscale approach from subspecies to insular populations.Molecular Ecology,18(3):454-467.
García-Verdugo C,Meíndez M,Velaízquez-Rosas N,Balaguer L.2010.Contrasting patterns of morphological and physiological differentiation across insular environments:phenotypic variation and heritability of light-related traits in Olea europaea.Oecologia,164(3):647-655.
Ge Song,Wang Ming-xiu,Chen Yue-wu.1988.An analysis of population genetic structure of masson pine by isozyme technique.Scientia Silvae Sinicae,24:399-409.(in Chinese)葛颂,王明庥,陈岳武.1988.用同工酶研究马尾松群体的遗传结构.林业科学,24:399-409.
Hu Biao-lin,Wan Yong,Li Xia,Lei Jian-guo,Luo Xiang-dong,Yan Wen-gui,Xie Jian-kun.2012.Analysis on genetic diversity of phenotypic traits in rice(Oryza sativa)core collection and its comprehensive assessment.Acta Agronomica Sinica,38(5):829-839.(in Chinese)胡标林,万勇,李霞,雷建国,罗向东,严文贵,谢建坤.2012.水稻核心种质表型性状遗传多样性分析及综合评价.作物学报,38(5):829-839.
Huang Yong,Yao Xiao-hua,Wang Kai-liang,Ren Hua-dong,Li Zhi-zhen,Xie Yi-qing.2011.Genetic diversities of phenotypical traits of fruit and seed in Camellia meiocarpa Hu.Journal of Anhui Agricultural University,38(5):698-707.(in Chinese)黄勇,姚小华,王开良,任华东,李志真,谢一青.2011.小果油茶种实表型性状遗传多样性研究.安徽农业大学学报,38(5):698-707.
Jiang Xi-bing,Gong Bang-chu,Liu Qing-zhong,Chen Xin,Wu Kai-yun,Deng Quan-en,Tang Dan.2014.Phenotypic diversity of important agronomic traits of local cultivars of Chinese chestnut.Acta Horticulturae Sinica,41(4):641-652.(in Chinese)江锡兵,龚榜初,刘庆忠,陈新,吴开云,邓全恩,汤丹.2014.中国板栗地方品种重要农艺性状的表型多样性.园艺学报,41(4):641-652.
Kong Fan-ling.2006.Plant quantitative genetics.Beijing:China Agricultural University Press.(in Chinese)孔繁玲.2006.植物数量遗传学.北京:中国农业大学出版社.
Lan Yan-ping,Zhou Lian-di,Zhou Jia-hua,Yao Yan-wu,Wang Shang-de.2007.Researches on characteristics frequency of northern population in Castanea mollissima.Acta Agriculturae Boreali-Sinica,22(5):106-109.(in Chinese)兰彦平,周连第,周家华,姚砚武,王尚德.2007.中国板栗北方种群表型变异频率研究.华北农学报,22(5):106-109.
Lan Yan-ping,Zhou Lian-di,Yao Yan-wu,Wang Shang-de,Liu Guo-bin.2010.Analysis of Castanea mollissima germplasm resources by AFLP.Acta Horticulturae Sinica,37(9):1499-1506.(in Chinese)兰彦平,周连第,姚研武,王尚德,刘国彬.2010.中国板栗种质资源的AFLP分析.园艺学报,37(9):1499-1506.
Li Bin,Gu Wan-chun,Lu Bao-ming.2002.A study on phenotypic diversity of seeds and cones characteristics in Pinus bungeana.Biodiversity Science,10(2):181-188.(in Chinese)李斌,顾万春,卢宝明.2002.白皮松天然群体种实性状表型多样性研究.生物多样性,10(2):181-188.
Li M M,Cai Y L,Qian Z Q,Zhao G F.2009.Genetic diversity and differentiation in Chinese sour cherry Prunus pseudocerasus Lindl.,and its implications for conservation.Genetic Resources and Crop Evolution,56(4):455-464.
Liu Guo-bin,Lan Yan-ping,Lan Wei-zong,Cao Jun.2013.An analysis of phenotypic traits of native chestnut varieties.Journal of Jiangxi Agricultural University,35(5):977-981,987.(in Chinese)刘国彬,兰彦平,兰卫宗,曹均.2013.板栗农家品种资源坚果表型性状分析.江西农业大学学报,35(5):977-981,987.
Liu Guo-bin,Lan Yan-ping,Yao Yan-wu,Lan Wei-zong,Wang Jin-bao,Liu Jian-ling.2011.Genetic variation of nut morphological traits in crossed chestnut progenies.Acta Agriculturae Boreali-Sinica,26(5):117-121.(in Chinese)刘国彬,兰彦平,姚研武,兰卫宗,王金宝,刘建玲.2011.板栗杂交后代坚果表型性状的遗传变异.华北农学报,26(5):117-121.
Liu Qing-zhong.2006.Descriptors and data standard for chestnut(Castanea mollissima Blume).Beijing:China Agriculture Press:51.(in Chinese)刘庆忠.2006.板栗种质资源描述规范和数据标准.北京:中国农业出版社:51.
Ma Yu-min,Chen Xue-sen,He Tian-ming,Wu Chuan-jin,Wang Na.2008.Genetic diversity of morphological traits in wild populations of Castanea mollissima Blume.Acta Horticulturae Sinica,35(12):1717-1726.(in Chinese)马玉敏,陈学森,何天明,吴传金,王娜.2008.中国板栗3个野生居群部分表型性状的遗传多样性.园艺学报,35(12):1717-1726.
Mitchell-Olds T,Willis J H,Goldstein D B.2007.Which evolutionary processes influence natural genetic variation for phenotypic traits.Nature Reviews Genetics,8(11):845-856.
Shen Guang-ning.2015.The science and practice of fruit trees in China·Chestnut.Xi’an:Shaanxi Technological Press.(in Chinese)沈广宁.2015.中国果树科学与实践·板栗.西安:陕西科技出版社.
Wang Guang-peng,Kong De-jun,Liu Qing-xiang.2009.Inheritance characters of three economic characteristics of crossed chestnut progeny.Acta Agriculturae Boreali-Sinica,24(Supplement):102-104.(in Chinese)王广鹏,孔德军,刘庆香.2009.板栗杂交后代3个重要经济性状的遗传特点.华北农学报,24(增刊):102-104.
Wang Guang-peng,Lu Feng-qin,Kong De-jun.2016.Highly effective cultivation and control technique of main pests and diseases of chestnut.Beijing:China Agriculture Press:81-84.(in Chinese)王广鹏,陆凤勤,孔德军.2016.板栗高效栽培技术与主要病虫害防治.北京:中国农业出版社:81-84.
Wang Guang-peng,Liu Qing-xiang,Kong De-jun.2004.The path analysis of main quantitative characteristics on single plant yield of chestnut.Journal of Hebei Agricultural Sciences,8(3):60-62.(in Chinese)王广鹏,刘庆香,孔德军.2004.板栗主要园艺性状与单株产量的通径分析.河北农业科学,8(3):60-62.
Wang Ya-li,Li Yi,Chen Xiao-yang.2008.Phenotypic diversity of natural populations in Picea crassifolia in Qilian mountains.Scientia Silvae Sinicae,44(2):70-77.(in Chinese)王娅丽,李毅,陈晓阳.2008.祁连山青海云杉天然群体表型性状遗传多样性分析.林业科学,44(2):70-77.
Yang Yang,Guo Yan,Zhang Shu-hang,Li Ying,Zhang Xin-fang,Wang Guang-peng.2017.The origin,evolution and classification of chestnut germplasm resources in China.Journal of Hebei Agricultural Sciences,21(2):25-28.(in Chinese)杨阳,郭燕,张树航,李颖,张馨方,王广鹏.2017.中国栗属植物起源演化和分类研究进展.河北农业科学,21(2):25-28.
Zhang Xiang-qian,Liu Jing-hui,Qi Bing-jie,Guo Xiao-xia,Jiao Wei-hong.2010.Cluster diversity analysis of the main agronomic traits in oat germplasm.Journal of Plant Genetic Resources,11(2):168-174.(in Chinese)张向前,刘景辉,齐冰洁,郭晓霞,焦伟红.2010.燕麦种质资源主要农艺性状的遗传多样性分析.植物遗传资源学报,11(2):168-174.
Zhang Yu-he,Wang Fu-tang,Gao Xin-yi,Zhao Yong-xiao.1989.Chestnut.China Forestry Publishing House:52-61.(in Chinese)张宇和,王福堂,高新一,赵永孝.1989.板栗.北京:中国林业出版社:52-61.
Zhang Yun-yue,Ma Chang-geng,Lin Mu-jiu,Li Bai-hai.2001.Study on one of genetic variations for Ginkgo biloba in China the variation of breeding fruit-stone characters among and within population.Scientia Silvae sinicae,7(4):35-40.(in Chinese)张云跃,马常耕,林睦就,李柏海.2001.我国银杏遗传变异研究之一--种核性状的群体间和群体内变异.林业科学,37(4):35-40.
Zhou Lian-di,Lan Yan-ping,Cao Qing-chang,Li Shu-ying,Lan Wei-zong.2005.Geographical variation of morphologic characteristics of Castanea mollissima seeds and legumes.Chinese Agricultural Science Bulletin,21(9):136-139.(in Chinese)周连第,兰彦平,曹庆昌,李淑英,兰卫宗.2005.板栗叶片性状表型多样性研究.中国农学通报,21(9):136-139.
García-Verdugo C,Fay M F,Granado-Yela C,de Casas R Rubio,Balaguer L,Besnard G,Vargas P.2009.Genetic diversity and differentiation processes in the ploidy series of Olea europaea L.:a multiscale approach from subspecies to insular populations.Molecular Ecology,18(3):454-467.
García-Verdugo C,Meíndez M,Velaízquez-Rosas N,Balaguer L.2010.Contrasting patterns of morphological and physiological differentiation across insular environments:phenotypic variation and heritability of light-related traits in Olea europaea.Oecologia,164(3):647-655.
Ge Song,Wang Ming-xiu,Chen Yue-wu.1988.An analysis of population genetic structure of masson pine by isozyme technique.Scientia Silvae Sinicae,24:399-409.(in Chinese)葛颂,王明庥,陈岳武.1988.用同工酶研究马尾松群体的遗传结构.林业科学,24:399-409.
Hu Biao-lin,Wan Yong,Li Xia,Lei Jian-guo,Luo Xiang-dong,Yan Wen-gui,Xie Jian-kun.2012.Analysis on genetic diversity of phenotypic traits in rice(Oryza sativa)core collection and its comprehensive assessment.Acta Agronomica Sinica,38(5):829-839.(in Chinese)胡标林,万勇,李霞,雷建国,罗向东,严文贵,谢建坤.2012.水稻核心种质表型性状遗传多样性分析及综合评价.作物学报,38(5):829-839.
Huang Yong,Yao Xiao-hua,Wang Kai-liang,Ren Hua-dong,Li Zhi-zhen,Xie Yi-qing.2011.Genetic diversities of phenotypical traits of fruit and seed in Camellia meiocarpa Hu.Journal of Anhui Agricultural University,38(5):698-707.(in Chinese)黄勇,姚小华,王开良,任华东,李志真,谢一青.2011.小果油茶种实表型性状遗传多样性研究.安徽农业大学学报,38(5):698-707.
Jiang Xi-bing,Gong Bang-chu,Liu Qing-zhong,Chen Xin,Wu Kai-yun,Deng Quan-en,Tang Dan.2014.Phenotypic diversity of important agronomic traits of local cultivars of Chinese chestnut.Acta Horticulturae Sinica,41(4):641-652.(in Chinese)江锡兵,龚榜初,刘庆忠,陈新,吴开云,邓全恩,汤丹.2014.中国板栗地方品种重要农艺性状的表型多样性.园艺学报,41(4):641-652.
Kong Fan-ling.2006.Plant quantitative genetics.Beijing:China Agricultural University Press.(in Chinese)孔繁玲.2006.植物数量遗传学.北京:中国农业大学出版社.
Lan Yan-ping,Zhou Lian-di,Zhou Jia-hua,Yao Yan-wu,Wang Shang-de.2007.Researches on characteristics frequency of northern population in Castanea mollissima.Acta Agriculturae Boreali-Sinica,22(5):106-109.(in Chinese)兰彦平,周连第,周家华,姚砚武,王尚德.2007.中国板栗北方种群表型变异频率研究.华北农学报,22(5):106-109.
Lan Yan-ping,Zhou Lian-di,Yao Yan-wu,Wang Shang-de,Liu Guo-bin.2010.Analysis of Castanea mollissima germplasm resources by AFLP.Acta Horticulturae Sinica,37(9):1499-1506.(in Chinese)兰彦平,周连第,姚研武,王尚德,刘国彬.2010.中国板栗种质资源的AFLP分析.园艺学报,37(9):1499-1506.
Li Bin,Gu Wan-chun,Lu Bao-ming.2002.A study on phenotypic diversity of seeds and cones characteristics in Pinus bungeana.Biodiversity Science,10(2):181-188.(in Chinese)李斌,顾万春,卢宝明.2002.白皮松天然群体种实性状表型多样性研究.生物多样性,10(2):181-188.
Li M M,Cai Y L,Qian Z Q,Zhao G F.2009.Genetic diversity and differentiation in Chinese sour cherry Prunus pseudocerasus Lindl.,and its implications for conservation.Genetic Resources and Crop Evolution,56(4):455-464.
Liu Guo-bin,Lan Yan-ping,Lan Wei-zong,Cao Jun.2013.An analysis of phenotypic traits of native chestnut varieties.Journal of Jiangxi Agricultural University,35(5):977-981,987.(in Chinese)刘国彬,兰彦平,兰卫宗,曹均.2013.板栗农家品种资源坚果表型性状分析.江西农业大学学报,35(5):977-981,987.
Liu Guo-bin,Lan Yan-ping,Yao Yan-wu,Lan Wei-zong,Wang Jin-bao,Liu Jian-ling.2011.Genetic variation of nut morphological traits in crossed chestnut progenies.Acta Agriculturae Boreali-Sinica,26(5):117-121.(in Chinese)刘国彬,兰彦平,姚研武,兰卫宗,王金宝,刘建玲.2011.板栗杂交后代坚果表型性状的遗传变异.华北农学报,26(5):117-121.
Liu Qing-zhong.2006.Descriptors and data standard for chestnut(Castanea mollissima Blume).Beijing:China Agriculture Press:51.(in Chinese)刘庆忠.2006.板栗种质资源描述规范和数据标准.北京:中国农业出版社:51.
Ma Yu-min,Chen Xue-sen,He Tian-ming,Wu Chuan-jin,Wang Na.2008.Genetic diversity of morphological traits in wild populations of Castanea mollissima Blume.Acta Horticulturae Sinica,35(12):1717-1726.(in Chinese)马玉敏,陈学森,何天明,吴传金,王娜.2008.中国板栗3个野生居群部分表型性状的遗传多样性.园艺学报,35(12):1717-1726.
Mitchell-Olds T,Willis J H,Goldstein D B.2007.Which evolutionary processes influence natural genetic variation for phenotypic traits.Nature Reviews Genetics,8(11):845-856.
Shen Guang-ning.2015.The science and practice of fruit trees in China·Chestnut.Xi’an:Shaanxi Technological Press.(in Chinese)沈广宁.2015.中国果树科学与实践·板栗.西安:陕西科技出版社.
Wang Guang-peng,Kong De-jun,Liu Qing-xiang.2009.Inheritance characters of three economic characteristics of crossed chestnut progeny.Acta Agriculturae Boreali-Sinica,24(Supplement):102-104.(in Chinese)王广鹏,孔德军,刘庆香.2009.板栗杂交后代3个重要经济性状的遗传特点.华北农学报,24(增刊):102-104.
Wang Guang-peng,Lu Feng-qin,Kong De-jun.2016.Highly effective cultivation and control technique of main pests and diseases of chestnut.Beijing:China Agriculture Press:81-84.(in Chinese)王广鹏,陆凤勤,孔德军.2016.板栗高效栽培技术与主要病虫害防治.北京:中国农业出版社:81-84.
Wang Guang-peng,Liu Qing-xiang,Kong De-jun.2004.The path analysis of main quantitative characteristics on single plant yield of chestnut.Journal of Hebei Agricultural Sciences,8(3):60-62.(in Chinese)王广鹏,刘庆香,孔德军.2004.板栗主要园艺性状与单株产量的通径分析.河北农业科学,8(3):60-62.
Wang Ya-li,Li Yi,Chen Xiao-yang.2008.Phenotypic diversity of natural populations in Picea crassifolia in Qilian mountains.Scientia Silvae Sinicae,44(2):70-77.(in Chinese)王娅丽,李毅,陈晓阳.2008.祁连山青海云杉天然群体表型性状遗传多样性分析.林业科学,44(2):70-77.
Yang Yang,Guo Yan,Zhang Shu-hang,Li Ying,Zhang Xin-fang,Wang Guang-peng.2017.The origin,evolution and classification of chestnut germplasm resources in China.Journal of Hebei Agricultural Sciences,21(2):25-28.(in Chinese)杨阳,郭燕,张树航,李颖,张馨方,王广鹏.2017.中国栗属植物起源演化和分类研究进展.河北农业科学,21(2):25-28.
Zhang Xiang-qian,Liu Jing-hui,Qi Bing-jie,Guo Xiao-xia,Jiao Wei-hong.2010.Cluster diversity analysis of the main agronomic traits in oat germplasm.Journal of Plant Genetic Resources,11(2):168-174.(in Chinese)张向前,刘景辉,齐冰洁,郭晓霞,焦伟红.2010.燕麦种质资源主要农艺性状的遗传多样性分析.植物遗传资源学报,11(2):168-174.
Zhang Yu-he,Wang Fu-tang,Gao Xin-yi,Zhao Yong-xiao.1989.Chestnut.China Forestry Publishing House:52-61.(in Chinese)张宇和,王福堂,高新一,赵永孝.1989.板栗.北京:中国林业出版社:52-61.
Zhang Yun-yue,Ma Chang-geng,Lin Mu-jiu,Li Bai-hai.2001.Study on one of genetic variations for Ginkgo biloba in China the variation of breeding fruit-stone characters among and within population.Scientia Silvae sinicae,7(4):35-40.(in Chinese)张云跃,马常耕,林睦就,李柏海.2001.我国银杏遗传变异研究之一--种核性状的群体间和群体内变异.林业科学,37(4):35-40.
Zhou Lian-di,Lan Yan-ping,Cao Qing-chang,Li Shu-ying,Lan Wei-zong.2005.Geographical variation of morphologic characteristics of Castanea mollissima seeds and legumes.Chinese Agricultural Science Bulletin,21(9):136-139.(in Chinese)周连第,兰彦平,曹庆昌,李淑英,兰卫宗.2005.板栗叶片性状表型多样性研究.中国农学通报,21(9):136-139.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |