指纹图谱技术在葡萄及葡萄酒研究上的应用
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摘要
葡萄及葡萄酒品质难辨,易形成混乱的市场局面,制约了葡萄及葡萄酒产业现代化发展进程。近年来,指纹图谱技术在葡萄和葡萄酒研究上的应用越来越多,本文就DNA指纹图谱技术在葡萄种质资源领域的应用;色谱指纹图谱技术在葡萄酒品种、酒龄、产区及真伪鉴定方面的应用进行了综述,并对存在的问题进行了分析,对其今后的发展做出了展望,为指纹图谱技术在葡萄及葡萄酒研究上的进一步应用提供理论基础和参考依据。
Chaotic market situation was formed for identifying the quality of grapevine and wine difficultly, which affected the modern development of grapevine and wine industry. In recent years, more and more application of grapevine and wine was studied by fingerprinting techniques. DNA fingerprinting technique used in the field of grape germplasm resources. Chromatographic fingerprint technique was used in varieties of wine, wine ages, regions and authenticity. In this paper, the methods of fingerprint technique were reviewed, the existing problems were analyzed, and a prospect on the development of the future was made. The fingerprint technology in grape and wine research provided the theoretical basis and reference for the further application.
Chaotic market situation was formed for identifying the quality of grapevine and wine difficultly, which affected the modern development of grapevine and wine industry. In recent years, more and more application of grapevine and wine was studied by fingerprinting techniques. DNA fingerprinting technique used in the field of grape germplasm resources. Chromatographic fingerprint technique was used in varieties of wine, wine ages, regions and authenticity. In this paper, the methods of fingerprint technique were reviewed, the existing problems were analyzed, and a prospect on the development of the future was made. The fingerprint technology in grape and wine research provided the theoretical basis and reference for the further application.
引文
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[2]国家统计局.中国统计年鉴[J].北京:中国统计出版社,2017:560-570.
[3]任国慧,吴伟民,房经贵,等.我国葡萄国家级种质资源圃的建设现状[J].江西农业学报,2012,24(7):10-13.
[4]任继波,胡文效.葡萄籽蛋白质利用研究进展[J].中外葡萄与葡萄酒,2014(3):58-61.
[5]任继波,李敬龙,胡文效.葡萄籽蛋白质的分离纯化及电泳分析[J].江苏农业科学,2016,44(4):318-321.
[6]刘镇东.山葡萄高密度分子遗传图谱构建及抗寒性QTL定位研究[D].沈阳农业大学,2012.
[7]李霞.基于近红外光谱技术的赤霞珠干红葡萄酒品质指标检测方法研究[D].西北农林科技大学,2018.
[8]姚玉新,左方梅,翟衡,等.‘蛇龙珠’等酿酒葡萄品种的DNA分析[J].园艺学报,2005,32(4):604-608.
[9]LAUCOU V,BOURSIQUOT J M,LACOMBE T,et al.Parentage of grapevine rootstock'Feral'finally elucidated[J].Vitis,2008,47(3):163-167.
[10]史圆圆,张旭彤,王跃进,等.无核葡萄种质亲缘关系的SRAP研究[J].中国农学通报,2018,34(9):57-61.
[11]郭春苗,李宁,周晓明,等.基于SSR标记的葡萄品种(系)遗传多样性分析与指纹图谱构建[J].新疆农业科学,2015,52(11):2051-2058.
[12]尹玲,张晨,向江,等.我国新育成葡萄品种SSR指纹图谱的建立[J].果树学报,2015,32(3):366-373.
[13]张永辉,刘崇怀,樊秀彩,等.ISSR标记在中国野生葡萄分类中的应用[J].果树学报,2011,28(3):406-412.
[14]金炳奎,宗成文,曹后男,等.山葡萄SRAP技术体系的建立及其在品种鉴定中的应用[J].吉林农业大学学报,2013,35(2):198-205.
[15]DANIELA B,DORU P,ALEXANDRU B,et al.The polymorphic study of ten table grapes varieties using RAPD method[J].Bulletin UASVM Animal Science and Biotechnologies,2010,67:364-369.
[16]鲍露,徐昌杰,江文彬,等.葡萄AFLP技术体系建立及其在超藤与藤稔葡萄品种鉴别中的应用[J].果树学报,2005,22(4):422-425.
[17]温景辉,申海林,邹利人,等.20份葡萄种质亲缘关系的SSR分析[J].果树学报,2011,28(5):782-786.
[18]杨航宇,杨哲,何非,等.43份葡萄种质遗传多样性的SSR分析[J].中外葡萄与葡萄酒,2018(1):1-8.
[19]杜晶晶,刘国银,魏军亚,等.基于SSR标记构建葡萄种质资源分子身份证[J].植物研究,2013,33(2):232-237.
[20]EMANUELLI F,LORENZI S,GRZESKOWIAKL,et al.Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape[J].Bmc Plant Biology,2013,13(39):2-17.
[21]王慧玲,闫爱玲,孙磊,等.13个中国葡萄优新品种的分子身份证构建[J].生物技术通报,2016,32(4):137-142.
[22]董志刚,刘伟,李晓梅,等.15个酿酒葡萄品种的分子身份证构建[J].中国农业技术导报,2017,19(9):42-50.
[23]李贝贝,姜建福,张颖.葡萄品种DNA指纹数据库的构建及遗传多样性分析[J].植物遗传资源学报,2018,19(2):338-350.
[24]庞立,刘素纯,夏菠,等.指纹图谱在酒的检测中的作用[J].酿酒科技,2009(8):128-130.
[25]I?IGO AROZARENA,BELéN AYESTARáN,MARíACANTALEJO,et al.Anthocyanin composition of Tempranillo,Garnacha and Cabernet Sauvignon grapes from high-and lowquality vineyards over two years[J].European Food Research&Technology,2002,214(4):303-309.
[26]REVILLA E,GARCíA-BENEYTEZ F,CABELLO F,et al.Value of high-performance liquid chromatographic analysis of anthocyanins in the differentiation of red grape cultivars and red wines made from them[J].Journal of Chromatography A,2001,915(1):53-60.
[27]张军翔,冯长根,李华.不同品种红葡萄酒花色苷高效液相色谱指纹图谱识别[J].农业工程学报,2006,22(10):216-221.
[28]郭艳波.南方山葡萄酒品质控制关键技术研究及功能性评价[D].福建农林大学,2013.
[29]TSANOVA-SAVOVA S,DIMOVW S,RIBAROVA F.Anthocyanins and color variables of Bulgarian aged red wines[J].Journal of Food Composition and Analysis,2002,15:647-654.
[30]张军翔,冯长根,李华.蛇龙珠葡萄酒酒龄花青素高效液相色谱(HPLC)指纹图谱研究[J].中国农业科学,2006,39(7):1451-1456.
[31]李彪.基于指纹图谱的白酒类别定性研究[D].武汉轻工大学,2015.
[32]SCHLOSSER J,REYNOLDS A G,KING M,et al.Canadian terroir:sensory characterization of Chardonnay in the Niagara Peninsula[J].Food Research International,2005,38(1):11-18.
[33]王秀芹,张庆华,战吉成,等.产地与品种对葡萄酒中酚类物质含量的影响[J].食品科学,2009,30(21):113-118
[34]韩国民.葡萄酒多酚HPLC指纹图谱的初步研究[D].西北农林科技大学,2010.
[35]谢建军.气相色谱指纹图谱法进行红葡萄酒产地溯源表征[J].食品科学,2013,34(18):253-257.
[36]张宪臣,华洪波,张朋杰,等.两种特征提取物的指纹图谱技术溯源进口霞多丽干白葡萄酒产地[J].吉林农业大学学报,2015,37(2):242-249.
[37]莫寅斌.贺兰山东麓地区葡萄酒指纹图谱的研究[D].西北农林科技大学,2009.
[38]谢建军,陈小帆,相大鹏,等.气相色谱指纹图谱法快速鉴别红葡萄酒真假[J].酿酒科技,2012(9):107-111.
[2]国家统计局.中国统计年鉴[J].北京:中国统计出版社,2017:560-570.
[3]任国慧,吴伟民,房经贵,等.我国葡萄国家级种质资源圃的建设现状[J].江西农业学报,2012,24(7):10-13.
[4]任继波,胡文效.葡萄籽蛋白质利用研究进展[J].中外葡萄与葡萄酒,2014(3):58-61.
[5]任继波,李敬龙,胡文效.葡萄籽蛋白质的分离纯化及电泳分析[J].江苏农业科学,2016,44(4):318-321.
[6]刘镇东.山葡萄高密度分子遗传图谱构建及抗寒性QTL定位研究[D].沈阳农业大学,2012.
[7]李霞.基于近红外光谱技术的赤霞珠干红葡萄酒品质指标检测方法研究[D].西北农林科技大学,2018.
[8]姚玉新,左方梅,翟衡,等.‘蛇龙珠’等酿酒葡萄品种的DNA分析[J].园艺学报,2005,32(4):604-608.
[9]LAUCOU V,BOURSIQUOT J M,LACOMBE T,et al.Parentage of grapevine rootstock'Feral'finally elucidated[J].Vitis,2008,47(3):163-167.
[10]史圆圆,张旭彤,王跃进,等.无核葡萄种质亲缘关系的SRAP研究[J].中国农学通报,2018,34(9):57-61.
[11]郭春苗,李宁,周晓明,等.基于SSR标记的葡萄品种(系)遗传多样性分析与指纹图谱构建[J].新疆农业科学,2015,52(11):2051-2058.
[12]尹玲,张晨,向江,等.我国新育成葡萄品种SSR指纹图谱的建立[J].果树学报,2015,32(3):366-373.
[13]张永辉,刘崇怀,樊秀彩,等.ISSR标记在中国野生葡萄分类中的应用[J].果树学报,2011,28(3):406-412.
[14]金炳奎,宗成文,曹后男,等.山葡萄SRAP技术体系的建立及其在品种鉴定中的应用[J].吉林农业大学学报,2013,35(2):198-205.
[15]DANIELA B,DORU P,ALEXANDRU B,et al.The polymorphic study of ten table grapes varieties using RAPD method[J].Bulletin UASVM Animal Science and Biotechnologies,2010,67:364-369.
[16]鲍露,徐昌杰,江文彬,等.葡萄AFLP技术体系建立及其在超藤与藤稔葡萄品种鉴别中的应用[J].果树学报,2005,22(4):422-425.
[17]温景辉,申海林,邹利人,等.20份葡萄种质亲缘关系的SSR分析[J].果树学报,2011,28(5):782-786.
[18]杨航宇,杨哲,何非,等.43份葡萄种质遗传多样性的SSR分析[J].中外葡萄与葡萄酒,2018(1):1-8.
[19]杜晶晶,刘国银,魏军亚,等.基于SSR标记构建葡萄种质资源分子身份证[J].植物研究,2013,33(2):232-237.
[20]EMANUELLI F,LORENZI S,GRZESKOWIAKL,et al.Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape[J].Bmc Plant Biology,2013,13(39):2-17.
[21]王慧玲,闫爱玲,孙磊,等.13个中国葡萄优新品种的分子身份证构建[J].生物技术通报,2016,32(4):137-142.
[22]董志刚,刘伟,李晓梅,等.15个酿酒葡萄品种的分子身份证构建[J].中国农业技术导报,2017,19(9):42-50.
[23]李贝贝,姜建福,张颖.葡萄品种DNA指纹数据库的构建及遗传多样性分析[J].植物遗传资源学报,2018,19(2):338-350.
[24]庞立,刘素纯,夏菠,等.指纹图谱在酒的检测中的作用[J].酿酒科技,2009(8):128-130.
[25]I?IGO AROZARENA,BELéN AYESTARáN,MARíACANTALEJO,et al.Anthocyanin composition of Tempranillo,Garnacha and Cabernet Sauvignon grapes from high-and lowquality vineyards over two years[J].European Food Research&Technology,2002,214(4):303-309.
[26]REVILLA E,GARCíA-BENEYTEZ F,CABELLO F,et al.Value of high-performance liquid chromatographic analysis of anthocyanins in the differentiation of red grape cultivars and red wines made from them[J].Journal of Chromatography A,2001,915(1):53-60.
[27]张军翔,冯长根,李华.不同品种红葡萄酒花色苷高效液相色谱指纹图谱识别[J].农业工程学报,2006,22(10):216-221.
[28]郭艳波.南方山葡萄酒品质控制关键技术研究及功能性评价[D].福建农林大学,2013.
[29]TSANOVA-SAVOVA S,DIMOVW S,RIBAROVA F.Anthocyanins and color variables of Bulgarian aged red wines[J].Journal of Food Composition and Analysis,2002,15:647-654.
[30]张军翔,冯长根,李华.蛇龙珠葡萄酒酒龄花青素高效液相色谱(HPLC)指纹图谱研究[J].中国农业科学,2006,39(7):1451-1456.
[31]李彪.基于指纹图谱的白酒类别定性研究[D].武汉轻工大学,2015.
[32]SCHLOSSER J,REYNOLDS A G,KING M,et al.Canadian terroir:sensory characterization of Chardonnay in the Niagara Peninsula[J].Food Research International,2005,38(1):11-18.
[33]王秀芹,张庆华,战吉成,等.产地与品种对葡萄酒中酚类物质含量的影响[J].食品科学,2009,30(21):113-118
[34]韩国民.葡萄酒多酚HPLC指纹图谱的初步研究[D].西北农林科技大学,2010.
[35]谢建军.气相色谱指纹图谱法进行红葡萄酒产地溯源表征[J].食品科学,2013,34(18):253-257.
[36]张宪臣,华洪波,张朋杰,等.两种特征提取物的指纹图谱技术溯源进口霞多丽干白葡萄酒产地[J].吉林农业大学学报,2015,37(2):242-249.
[37]莫寅斌.贺兰山东麓地区葡萄酒指纹图谱的研究[D].西北农林科技大学,2009.
[38]谢建军,陈小帆,相大鹏,等.气相色谱指纹图谱法快速鉴别红葡萄酒真假[J].酿酒科技,2012(9):107-111.
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