荧光光谱法测定淀粉中的直链淀粉
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摘要
为了建立一种荧光光谱法测定淀粉中直链淀粉含量的方法,采用荧光光谱法测定了直链淀粉的激发光谱和发射光谱以及直链淀粉与支链淀粉的浓度比对荧光强度的影响。结果表明:直链淀粉的激发波长为370 nm、发射波长为422 nm;在0.10～0.70μg/mL浓度范围内,直链淀粉荧光强度与其浓度呈良好线性关系,相关系数r=0.9996,检出限为0.026μg/mL。用该方法测定玉米淀粉和土豆淀粉中直链淀粉的含量,加标回收率为94.5%～121.7%,相对标准偏差(RSD)为2.42%～3.24%。该方法不需加任何生化试剂,不受时间、温度、共存支链淀粉的影响,快速简便,绿色环保,检出限低,结果准确。
In order to establish method for determination of amylose contents in starch by fluorescence spectrometry. The excitation and emission spectra of amylose,and the effect of ratios of amylose to amylopectin on fluorescence intensity were determined by fluorescence spectrometry.The results showed that: The amyloseemitted fluorescence at theexcitation wavelengths of 370 nm and emission wavelengths of 422 nm.In the concentration range of 0.10～0.70 μg/mL,the fluorescence intensity of amylose presented a good linear relationship with its concentration,and the correlation coefficients was 0.9996,and the detection limit was 0.026 μg/mL.The method was applied to the determination of amylose contents in corn starchand potato with recoveries 94.6% ～121.7%,and the RSD was 2.42% ～3.24%.This method didn't need any biochemical reagents,and had no affected by time,temperature,coexistence of amylopectin,which was fast and simple,green and environmental friendly,low detection limit with accurate results.
In order to establish method for determination of amylose contents in starch by fluorescence spectrometry. The excitation and emission spectra of amylose,and the effect of ratios of amylose to amylopectin on fluorescence intensity were determined by fluorescence spectrometry.The results showed that: The amyloseemitted fluorescence at theexcitation wavelengths of 370 nm and emission wavelengths of 422 nm.In the concentration range of 0.10～0.70 μg/mL,the fluorescence intensity of amylose presented a good linear relationship with its concentration,and the correlation coefficients was 0.9996,and the detection limit was 0.026 μg/mL.The method was applied to the determination of amylose contents in corn starchand potato with recoveries 94.6% ～121.7%,and the RSD was 2.42% ～3.24%.This method didn't need any biochemical reagents,and had no affected by time,temperature,coexistence of amylopectin,which was fast and simple,green and environmental friendly,low detection limit with accurate results.
引文
[1]余飞,邓丹雯,董婧,等.直链淀粉含量的影响因素及其应用研究进展[J].食品科学,2007,28(10):604-608.
[2]王中荣,刘雄.高直链淀粉性质及应用研究[J].粮食与油脂,2005(11):10-13.
[3]杨有仙,赵燕,李建科,等.直链淀粉含量测定方法研究进展[J].食品科学,2010,31(23):417-422.
[4]Williams P C,Kuzina F D,Hlynka I. A rapid colorimetric method for estimating the amylose content of starches and flours[J].Cereal Chemistry,1970,47(4):411-420.
[5]曹忙选.电位滴定法快速测定直链淀粉含量[J].西北农业学报,2003,12(4):91-92,97.
[6]金玉红,张开利,张兴春,等.双波长法测定小麦及小麦芽中直链、支链淀粉含量[J].中国粮油学报,2009,24(1):137-140.
[7]李海普,李彬,欧阳明,等.直链淀粉和支链淀粉的表征[J].食品科学,2010,31(11):273-277.
[8]Grantl A,Ostenson A M,Rayas P.Determination of amylose and amylopectin of wheat starch using high performance sizeexclusion chromatography(HPSEC)[J]. Cereal Chemistry,2002,79:771-773.
[9]谢新华,肖昕,李晓芳,等.用近红外透射光谱技术测定精米直链淀粉含量研究[J].食品科学,2004(1):118-121.
[10]倪小英,刘荣,张晓燕,等.SKALAR SAN+化学自动分析仪在直链淀粉分析中的应用[J].粮食科技与经济,2009(3):41-42.
[11]Charoenkuln,Uttapap D,Pathipanawat W,et al.Simultaneous determination of amylose content&unit chain distribution of amylopectins of cassava starches by fluorescent labeling/HPSEC[J].Carbohydrate Polymers,2006,65(1):102-108.
[12]高若梅,刘鸿皋.检出限概念问题讨论—IUPA及其它检出限定义的综合探讨和实验论证[J].分析化学,1993,21(10):1232-1236.
[13]李满秀,张文琴.荧光光谱法测定残杀威的研究[J].分析科学学报,2009,25(2):235-237.
[14]何海建.淀粉荧光光谱特性的研究[D].无锡:江南大学,2006.
[15]GB 7648-87水稻、玉米、谷子籽粒直链淀粉含量的测定[S].北京:中国标准出版社,1998:309-313.
[2]王中荣,刘雄.高直链淀粉性质及应用研究[J].粮食与油脂,2005(11):10-13.
[3]杨有仙,赵燕,李建科,等.直链淀粉含量测定方法研究进展[J].食品科学,2010,31(23):417-422.
[4]Williams P C,Kuzina F D,Hlynka I. A rapid colorimetric method for estimating the amylose content of starches and flours[J].Cereal Chemistry,1970,47(4):411-420.
[5]曹忙选.电位滴定法快速测定直链淀粉含量[J].西北农业学报,2003,12(4):91-92,97.
[6]金玉红,张开利,张兴春,等.双波长法测定小麦及小麦芽中直链、支链淀粉含量[J].中国粮油学报,2009,24(1):137-140.
[7]李海普,李彬,欧阳明,等.直链淀粉和支链淀粉的表征[J].食品科学,2010,31(11):273-277.
[8]Grantl A,Ostenson A M,Rayas P.Determination of amylose and amylopectin of wheat starch using high performance sizeexclusion chromatography(HPSEC)[J]. Cereal Chemistry,2002,79:771-773.
[9]谢新华,肖昕,李晓芳,等.用近红外透射光谱技术测定精米直链淀粉含量研究[J].食品科学,2004(1):118-121.
[10]倪小英,刘荣,张晓燕,等.SKALAR SAN+化学自动分析仪在直链淀粉分析中的应用[J].粮食科技与经济,2009(3):41-42.
[11]Charoenkuln,Uttapap D,Pathipanawat W,et al.Simultaneous determination of amylose content&unit chain distribution of amylopectins of cassava starches by fluorescent labeling/HPSEC[J].Carbohydrate Polymers,2006,65(1):102-108.
[12]高若梅,刘鸿皋.检出限概念问题讨论—IUPA及其它检出限定义的综合探讨和实验论证[J].分析化学,1993,21(10):1232-1236.
[13]李满秀,张文琴.荧光光谱法测定残杀威的研究[J].分析科学学报,2009,25(2):235-237.
[14]何海建.淀粉荧光光谱特性的研究[D].无锡:江南大学,2006.
[15]GB 7648-87水稻、玉米、谷子籽粒直链淀粉含量的测定[S].北京:中国标准出版社,1998:309-313.
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