凹凸棒石缓解热处理豆粕对肉鸡氧化应激的研究
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摘要
本实验旨在研究日粮中添加凹凸棒石对摄入热处理豆粕肉鸡生产性能、血清生化和抗氧化能力的影响。将144只1日龄健康的爱拔益加肉鸡随机分为3组,每组6个重复,每个重复8只鸡,对照组肉鸡饲喂正常豆粕配制的基础日粮,实验1组所用豆粕为100℃加热处理8 h的豆粕,实验2组在实验1组的基础上添加1%凹凸棒石。结果表明:与对照组相比,饲喂热处理豆粕提高了肉鸡前期和全期料重比,提高了21 d回肠黏膜和42 d肝脏羧基含量(P <0. 05),添加凹凸棒石后,上述指标均恢复至对照组水平(P>0. 05)。此外,与对照组和实验1组相比,添加凹凸棒石可提高肉鸡前期平均日增重和21、42 d肝脏总超氧化物歧化酶活性,降低21 d血清丙二醛含量、21 d空肠黏膜羧基含量(P <0.05)。结果提示,添加凹凸棒石可改善饲喂热处理豆粕肉鸡的生产性能和抗氧化能力。
The aim of this experiment was to study the effects of Palygorskite supplementation on growth performance, serum biochemical index, and antioxidant capacity of broilers fed with heat-treated soybean meal. A total of 144 one-day-old healthy Arbor Acres broiler chicks were randomly divided into 3 groups with 6 replicates of 8 chicks each. Broilers in the control group were fed a basal diet with normal soybean meal, while chicks in the test groups were given heat-treated soybean meal diet(test group 1: soybean meal treated at 100 ℃ for 8 h) and the heat-treated soybean meal diet supplemented with 1 % palygorskite(test group 2). Compared with the control group, feeding heat-treated soybean meal increased feed to gain ratio of broilers during the early and overall period,and also increased protein carbonyl contents in the ileal mucosa at 21 d as well as liver at 42 d(P <0. 05), which were normalized to the levels comparable with the control group after Palygorskite inclusion( P > 0. 05). Moreover,the supplementation of Palygorskite promoted average daily gain of broilers during the early period, and total superoxide dismutase activity in the liver at both 21 and 42 d, whereas decreased malondialdehyde accumulation in the serum at 21 d and protein carbonyl content in the jejunal mucosa at 21 d when compared with the other two groups(P <0. 05). The results suggested that Palygorskite supplementation can improve growth performance and antioxidant capacity of broilers fed with heat-treated soybean meal.
The aim of this experiment was to study the effects of Palygorskite supplementation on growth performance, serum biochemical index, and antioxidant capacity of broilers fed with heat-treated soybean meal. A total of 144 one-day-old healthy Arbor Acres broiler chicks were randomly divided into 3 groups with 6 replicates of 8 chicks each. Broilers in the control group were fed a basal diet with normal soybean meal, while chicks in the test groups were given heat-treated soybean meal diet(test group 1: soybean meal treated at 100 ℃ for 8 h) and the heat-treated soybean meal diet supplemented with 1 % palygorskite(test group 2). Compared with the control group, feeding heat-treated soybean meal increased feed to gain ratio of broilers during the early and overall period,and also increased protein carbonyl contents in the ileal mucosa at 21 d as well as liver at 42 d(P <0. 05), which were normalized to the levels comparable with the control group after Palygorskite inclusion( P > 0. 05). Moreover,the supplementation of Palygorskite promoted average daily gain of broilers during the early period, and total superoxide dismutase activity in the liver at both 21 and 42 d, whereas decreased malondialdehyde accumulation in the serum at 21 d and protein carbonyl content in the jejunal mucosa at 21 d when compared with the other two groups(P <0. 05). The results suggested that Palygorskite supplementation can improve growth performance and antioxidant capacity of broilers fed with heat-treated soybean meal.
引文
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[4]SANTE-LHOUTELLIER V, AUBRY L, GATELLIER P.Effect of oxidation on in vitro digestibility of skeletal musclemyofibrillar proteins[J]. Journal of Agricultural and Food Chemistry, 2007, 55(13):5343-5348
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[7]罗丽琼,陈宗瑜,古今,等.紫外线-B辐射对植物DNA及蛋白质的影响[J].生态学杂志,2006(05):572-576LUO Liqiong, CHEN Zongyu, GU Ji, et al. Effects of enhanced UV-B radiation on protein synthesis and DNA damage in plants[J]. Chinese Journal of Ecology, 2006(5):572-576
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[10]TANG X, WU Q, LE G, et al. Effects of heat treatment on structural modification and in vivo antioxidant capacity of soy protein[J]. Nutrition, 2012, 28(11-12):1180-1185
[11]ZHANG X L, LU P, XUE W Y, et al. An evaluation of heat on protein oxidation of soy protein isolate or soy protein isolate mixed with soybean oil in vitro and its consequences on redox status of broilers at early age[J]. Asian-Australasian Journal of Animal Sciences. 2017. 30(8):1135-1142
[12]MURRAY H H. Traditional and new applications for kaolin, smectite,and palygorskite:a general overview[J].Applied Clay Science, 2000, 17(5-6):207-221
[13]PAPPAS A C, ZOIDIS E, THEOPHILOU N, et al. Effects of palygorskite on broiler performance, feed technological characteristics and litter quality[J]. Applied Clay Science,2010, 49(3):276-280
[14]张磊.凹凸棒石对肉鸡饲料制粒质量与肠道功能的影响及其相关机制研究[D].南京:南京农业大学,2016ZHANG Lei. A study on mechanism of palygorskite on feed pellet qualty and intestinal protection in broilers[D]. Nanjing:Nanjing Agricultural University, 2016
[15]CERVINI-SILVA J, NIETO-C.AMACHO A, GOMEZVIDALES V. Oxidative stress inhibition and oxidant activity by fibrous clays[J]. Colloids and Surface B:Biointerfaces,2015, 133:32-35
[16]SARIBEYOGLU K. AYTAC E.PEKMEZCI S.et al.Effects of clinoptilolite treatment on oxidative stress after partial hepatectomy in rats[J]. Asian Journal of Surgery,2011, 34(4):153-157
[17]CHEN Y P, CHENG Y F, LI X H, et al. Dietary palygorskite supplementation improves immunity, oxidative status,intestinal integrity, and barrier function of broilers at early age[J]. Animal Feed Science and Technology, 2016,219:200-209
[18]HENDRIKS W H, EMMENS M M, TRASS B, et al. Heat processing changes the protein quality of canned cat foods as measured with a rat bioassay[J]. Journal of Animal Science, 1999, 77(3):669-676
[19]LU P, ZHANG X L, XUE W Y, et al. The protein oxidation of soybean meal induced by heating decreases its protein digestion in vitro and impairs growth performance and digestive function in broilers[J]. British Poultry Science. 2017,58(6):704-711
[20]WU D W, CHEN X, YANG X, et al. Effects of heat treatment of soy protein isolate on the growth performance and immune function ofbroiler chickens[J]. Poultry Science,2014, 93(2):326-334
[21]ZHANG L, YAN R, ZHANG R Q, et al. Effect of different levels of palygorskite inclusion on pellet quality, growth performance and nutrient utilization in broilers[J]. Animal Feed Scienceand Technology, 2017, 223:73-81
[22]TANG C H. WANG X Q, ZHANG J M. Effects of supplemental palygorskite instead of zinc oxide on growth performance,apparent nutrient digestibility and fecal zinc excretion in weaned piglets[J].Animal Science Journal, 2014. 85(4):435-439
[23]张伟.沸石在畜牧养殖及饲料工业中的应用研究进展[J].当代畜禽养殖业,2003, 24(12):23-27ZHANG Wei. Research progress of application of zeolite in animal husbandry and feed industry[J]. Modern Animal Husbandly,2003, 24(12):23-27
[24]罗有文,周岩民,王恬.凹凸棒石粘土的生物学功能及其在动物生产上的应用[J].硅酸盐通报,2006, 25(6):159-164LUO Youwen, ZHOU Yanmin, WANG Tian. biological function and application of attapulgite in anmial Production[J]. Bulletin of the Chinese Ceramic Society, 2006, 25(6):159-164
[25]MORE J, FIORAMONTI J. BUENO L. Changes in gastrointestinal mucins caused by attapulgite Experimental studyin rats[J]. Gastroenterologie Clinique Et Biologique,1992,16(12):988-993
[26]王桂芳,吕宪俊,马少健.蒙脱石的药理作用与医学应用[J].中国非金属矿工业导刊,2009(3):14-16WANG Guifang, LU Xianjun, MA Shaojian. Pharmacological Action of Montmorillonite and Its Application in Medicine[J]. China Non-metallic Mining Industry Herald,2009(3):14-16
[27]OLIVER C N, AHN B W, MOERMAN E J, et al. Agerelated changes in oxidized proteins[J]. Journal of Biological Chemistry, 1987, 262(12):5488-5491
[28]LEWIS S E, BOYLE P M. MCKINNEY K A,et al. Total antioxidant capacity of seminal plasma is different in fertile and infertile men[J]. Fertility and Sterility, 1995, 64(4):868-870
[29]吴秋萍.氧化大豆蛋白对小鼠氧化还原状态及肝脏基因表达的影响[D].无锡:江南大学,2010WU Qiuping. Effect of oxidized soybean protein on the redox state and liver gene expression in mice[D]. Wuxi:Jiangnan University, 2010
[30]罗有文,王龙昌,周岩民,等.沸石、凹凸棒石黏土对肉鸡抗氧化性能和组织胆固醇的影响[J].粮食与饲料工业,2006(12):37-39LUO Youwen, WANG Longchang, ZHOU Yanmin, et al.Effects of zeolite, attapulgite on anti-oxidant capacity and tissue cholesterol of broilers[J]. Cereal&Feed Industry,2006(12):37-39
[31]KESHAVARZIAN A, FARHADI A, FORSYTH C B, et al.Evidence that chronic alcohol exposure promotes intestinal oxidative stress, intestinal hyperpermeability and endotoxemia prior to development of alcoholic steatohepatitis in rats[J]. Journal of Hepatology, 2009, 50(3):538-547
[32]尹彩娜.不同油脂对小鼠氧化应激及血脂代谢的影响[D].无锡:江南大学,2008YIN Caina. Dietary selenium and vitamin E alleviate dexinduced oxidative stress in broilers[D]. Wuxi:Jiangnan University, 2008
[33]WANG J P, CHI F, KIM I H. Effects of montmorillonite clay on growth performance, nutrient digestibility, vulva size, faecal microflora, and oxidative stress in weaning gilts challenged with zearalenone[J]. Animal Feed Science and Technology, 2012, 178(3-4):158-166.
[2]HEADLAM H A, DAVIES M J. Markers of protein oxidation:different oxidants give rise to variable yields of bound and released carbonyl products[J]. Free Radical Biology and Medicine, 2004. 36(9):1175-1184
[3]CHEN N, ZHAO M, SUN W, et al. Effect of oxidation on the emulsifying properties of soy protein isolate[J]. Food Research International, 2013, 52(1):26-32
[4]SANTE-LHOUTELLIER V, AUBRY L, GATELLIER P.Effect of oxidation on in vitro digestibility of skeletal musclemyofibrillar proteins[J]. Journal of Agricultural and Food Chemistry, 2007, 55(13):5343-5348
[5]TANG X, WU Q, LE G, et al. Structural and antioxidant modification of wheat peptides modified by the heat and lpid peroxidation poduct malondialdehyde[J]. Journal of Food Science, 2012, 77(1):H16-H22
[6]吴大伟.热氧化大豆蛋白对肉鸡的影响及维生素E和硫辛酸的调控研究[D].南京:南京农业大学,2014WU Dawei. Effects of soy protein oxidation by heating on broilers and the regulation by vitamin E and lipoic acid[D].Nanjing:Nanjing Agricultural University, 2014
[7]罗丽琼,陈宗瑜,古今,等.紫外线-B辐射对植物DNA及蛋白质的影响[J].生态学杂志,2006(05):572-576LUO Liqiong, CHEN Zongyu, GU Ji, et al. Effects of enhanced UV-B radiation on protein synthesis and DNA damage in plants[J]. Chinese Journal of Ecology, 2006(5):572-576
[8]MARCO M D, MARTINEZ S, HERNANDEZ F, et al. Nutritional value of two insect larval meals(Tenebrio molitor and Hernetia illucens)for broiler chickens:Apparent nutrient digestibility, apparent ileal amino acid digestibility and apparent metabolizable energy[J]. Animal Feed Science and Technology, 2015, 209:211-218
[9]吴大伟,陈星,洑琴,等.常温储存和干热处理对豆粕蛋白质氧化的影响[J].中国粮油学报,2014, 29(5):90-94WU Dawei, CHEN Xing, FU Qin, et al. Effects of storing and heating on protein oxidation of soybean meal[J]. Journal of the Chinese Cereals and Oils Association, 2014, 29(5):90-94
[10]TANG X, WU Q, LE G, et al. Effects of heat treatment on structural modification and in vivo antioxidant capacity of soy protein[J]. Nutrition, 2012, 28(11-12):1180-1185
[11]ZHANG X L, LU P, XUE W Y, et al. An evaluation of heat on protein oxidation of soy protein isolate or soy protein isolate mixed with soybean oil in vitro and its consequences on redox status of broilers at early age[J]. Asian-Australasian Journal of Animal Sciences. 2017. 30(8):1135-1142
[12]MURRAY H H. Traditional and new applications for kaolin, smectite,and palygorskite:a general overview[J].Applied Clay Science, 2000, 17(5-6):207-221
[13]PAPPAS A C, ZOIDIS E, THEOPHILOU N, et al. Effects of palygorskite on broiler performance, feed technological characteristics and litter quality[J]. Applied Clay Science,2010, 49(3):276-280
[14]张磊.凹凸棒石对肉鸡饲料制粒质量与肠道功能的影响及其相关机制研究[D].南京:南京农业大学,2016ZHANG Lei. A study on mechanism of palygorskite on feed pellet qualty and intestinal protection in broilers[D]. Nanjing:Nanjing Agricultural University, 2016
[15]CERVINI-SILVA J, NIETO-C.AMACHO A, GOMEZVIDALES V. Oxidative stress inhibition and oxidant activity by fibrous clays[J]. Colloids and Surface B:Biointerfaces,2015, 133:32-35
[16]SARIBEYOGLU K. AYTAC E.PEKMEZCI S.et al.Effects of clinoptilolite treatment on oxidative stress after partial hepatectomy in rats[J]. Asian Journal of Surgery,2011, 34(4):153-157
[17]CHEN Y P, CHENG Y F, LI X H, et al. Dietary palygorskite supplementation improves immunity, oxidative status,intestinal integrity, and barrier function of broilers at early age[J]. Animal Feed Science and Technology, 2016,219:200-209
[18]HENDRIKS W H, EMMENS M M, TRASS B, et al. Heat processing changes the protein quality of canned cat foods as measured with a rat bioassay[J]. Journal of Animal Science, 1999, 77(3):669-676
[19]LU P, ZHANG X L, XUE W Y, et al. The protein oxidation of soybean meal induced by heating decreases its protein digestion in vitro and impairs growth performance and digestive function in broilers[J]. British Poultry Science. 2017,58(6):704-711
[20]WU D W, CHEN X, YANG X, et al. Effects of heat treatment of soy protein isolate on the growth performance and immune function ofbroiler chickens[J]. Poultry Science,2014, 93(2):326-334
[21]ZHANG L, YAN R, ZHANG R Q, et al. Effect of different levels of palygorskite inclusion on pellet quality, growth performance and nutrient utilization in broilers[J]. Animal Feed Scienceand Technology, 2017, 223:73-81
[22]TANG C H. WANG X Q, ZHANG J M. Effects of supplemental palygorskite instead of zinc oxide on growth performance,apparent nutrient digestibility and fecal zinc excretion in weaned piglets[J].Animal Science Journal, 2014. 85(4):435-439
[23]张伟.沸石在畜牧养殖及饲料工业中的应用研究进展[J].当代畜禽养殖业,2003, 24(12):23-27ZHANG Wei. Research progress of application of zeolite in animal husbandry and feed industry[J]. Modern Animal Husbandly,2003, 24(12):23-27
[24]罗有文,周岩民,王恬.凹凸棒石粘土的生物学功能及其在动物生产上的应用[J].硅酸盐通报,2006, 25(6):159-164LUO Youwen, ZHOU Yanmin, WANG Tian. biological function and application of attapulgite in anmial Production[J]. Bulletin of the Chinese Ceramic Society, 2006, 25(6):159-164
[25]MORE J, FIORAMONTI J. BUENO L. Changes in gastrointestinal mucins caused by attapulgite Experimental studyin rats[J]. Gastroenterologie Clinique Et Biologique,1992,16(12):988-993
[26]王桂芳,吕宪俊,马少健.蒙脱石的药理作用与医学应用[J].中国非金属矿工业导刊,2009(3):14-16WANG Guifang, LU Xianjun, MA Shaojian. Pharmacological Action of Montmorillonite and Its Application in Medicine[J]. China Non-metallic Mining Industry Herald,2009(3):14-16
[27]OLIVER C N, AHN B W, MOERMAN E J, et al. Agerelated changes in oxidized proteins[J]. Journal of Biological Chemistry, 1987, 262(12):5488-5491
[28]LEWIS S E, BOYLE P M. MCKINNEY K A,et al. Total antioxidant capacity of seminal plasma is different in fertile and infertile men[J]. Fertility and Sterility, 1995, 64(4):868-870
[29]吴秋萍.氧化大豆蛋白对小鼠氧化还原状态及肝脏基因表达的影响[D].无锡:江南大学,2010WU Qiuping. Effect of oxidized soybean protein on the redox state and liver gene expression in mice[D]. Wuxi:Jiangnan University, 2010
[30]罗有文,王龙昌,周岩民,等.沸石、凹凸棒石黏土对肉鸡抗氧化性能和组织胆固醇的影响[J].粮食与饲料工业,2006(12):37-39LUO Youwen, WANG Longchang, ZHOU Yanmin, et al.Effects of zeolite, attapulgite on anti-oxidant capacity and tissue cholesterol of broilers[J]. Cereal&Feed Industry,2006(12):37-39
[31]KESHAVARZIAN A, FARHADI A, FORSYTH C B, et al.Evidence that chronic alcohol exposure promotes intestinal oxidative stress, intestinal hyperpermeability and endotoxemia prior to development of alcoholic steatohepatitis in rats[J]. Journal of Hepatology, 2009, 50(3):538-547
[32]尹彩娜.不同油脂对小鼠氧化应激及血脂代谢的影响[D].无锡:江南大学,2008YIN Caina. Dietary selenium and vitamin E alleviate dexinduced oxidative stress in broilers[D]. Wuxi:Jiangnan University, 2008
[33]WANG J P, CHI F, KIM I H. Effects of montmorillonite clay on growth performance, nutrient digestibility, vulva size, faecal microflora, and oxidative stress in weaning gilts challenged with zearalenone[J]. Animal Feed Science and Technology, 2012, 178(3-4):158-166.
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