黄麻链霉菌AUH-1发酵液的抗菌活性及其稳定性研究
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摘要
旨在研究黄麻链霉菌AUH-1菌株发酵液的抗真菌活性,并明确温度、紫外线及pH等理化因素对活性物质稳定性的影响。以8种植物病原真菌为供试菌,采用抑制菌丝生长速率法测定发酵液的抗真菌活性,并以水稻纹枯病菌为指示菌,测定不同条件下发酵液的抑菌活性。抗真菌活性结果表明:AUH-1菌株发酵液对8种植物病原真菌均具有不同程度的抑制作用,其中对西瓜枯萎病菌、烟草黑胫病菌、水稻纹枯病菌以及辣椒疫病菌的抑制效率分别高达81%、78%、68%和67%以上。稳定性实验结果表明:AUH-1菌株所产抗真菌活性物质具有良好的热稳定性和紫外稳定性。可见,黄麻链霉菌AUH-1具有广谱拮抗作用,具备开发生物农药的良好应用前景。
This work aims to study antifungal activity of fermentation broth from Streptomyces corchorusii AUH-1,and to verify the effects of biochemical and physical factors such as temperature,pH and ultraviolet on the stability of antifungal active substances. With 8 plant pathogenic fungi as the tested phytopathogen,inhibition rate of mycelial growth was used to determine antifungal activities of fermentation broth.With Rhizoctonia solani as indicator phytopathogen,the antifungal activities of fermentation broth under different conditions were measured.The antifungal activity results showed that the fermentation broth of strain AUH-1 had inhibitory effects on 8 pathogenic fungi at varied degrees.The inhibition rate of the fermentation broth to Fusarium oxysporum f.sp. niveum,Phytophthora parasitica var. nicotianae,Rhizoctonia solani,and Phytophthora capsici was over 81%,78%,68%,and 67%,respectively. The stability test showed that the antifungal active substance produced by this bacterium had favorable temperature stability and UV stability. The above results suggest that S. corchorusii AUH-1 have broadspectrum in suppressing the growth of various fungal plant pathogens,and have promising prospects for the development of microbial pesticides.
This work aims to study antifungal activity of fermentation broth from Streptomyces corchorusii AUH-1,and to verify the effects of biochemical and physical factors such as temperature,pH and ultraviolet on the stability of antifungal active substances. With 8 plant pathogenic fungi as the tested phytopathogen,inhibition rate of mycelial growth was used to determine antifungal activities of fermentation broth.With Rhizoctonia solani as indicator phytopathogen,the antifungal activities of fermentation broth under different conditions were measured.The antifungal activity results showed that the fermentation broth of strain AUH-1 had inhibitory effects on 8 pathogenic fungi at varied degrees.The inhibition rate of the fermentation broth to Fusarium oxysporum f.sp. niveum,Phytophthora parasitica var. nicotianae,Rhizoctonia solani,and Phytophthora capsici was over 81%,78%,68%,and 67%,respectively. The stability test showed that the antifungal active substance produced by this bacterium had favorable temperature stability and UV stability. The above results suggest that S. corchorusii AUH-1 have broadspectrum in suppressing the growth of various fungal plant pathogens,and have promising prospects for the development of microbial pesticides.
引文
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[12]赵娟,贾卫国,刘伟成,等.草莓灰霉病菌拮抗放线菌的筛选及活性测定[J].北方园艺, 2018, 42(4):59-65.
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[14]吴翔,甘炳成,黄忠乾,等. 2株降解纤维素放线菌的鉴定[J].西南农业学报, 2014, 27(1):117-120.
[15]Adinarayana G, Venkateshan MR, Bapiraju VVSNK, et al. Cytotoxic compounds from the marine actinobacterium Streptomyces corchorusii, AUBN 1/7 1[J]. Russian Journal of Bioorganic Chemistry, 2006, 32(3):295-300.
[2]俞晗.致病杆菌Xenorhabdus bovienii SN84次生代谢产物对三种植物病原真菌抑菌作用的研究[D].沈阳:沈阳农业大学,2017.
[3]毛良居,毛赫.链霉菌生物防治研究进展[J].安徽农业科学,2017, 45(1):145-147.
[4]招衡,张翼翾.生物农药及其未来研究和应用[J].世界农药,2010, 32(2):16-24.
[5]Deshpande BS, Ambedkar SS, Shewale JG. Biologically active secondary metabolites from Streptomyces[J]. Enzyme&Microbial Technology, 1988, 10(8):455-473.
[6]王敬伟,曾鑫年,林壁润.农用放线菌代谢产物研究概况[C].中国植物保护学会2007年学术年会, 2007.
[7]邱德文.生物农药研究进展与未来展望[J].植物保护, 2013,39(5):81-89.
[8]倪国荣,涂晓嵘,张智平,等.弗氏链霉菌S-221变种发酵液的抗菌活性及稳定性研究[J].中国酿造, 2009, 28(2):37-39.
[9]章帅文,杨勇,刘群,等.拮抗植物病原真菌Streptomyces corchorusii AUH-1的分离与鉴定[J].广东农业科学, 2018, 45(2):103-108.
[10]杨敬辉,吉沐祥,文平兰,等.黄麻链霉菌NF0919菌株对水稻纹枯病的生防活性[J].江苏农业学报, 2012, 28(6):1288-1293.
[11]杨敬辉,吉沐祥,陈宏州,等.黄麻链霉菌NF0919菌株发酵培养基的优化[J].江西农业学报, 2012, 24(10):136-139.
[12]赵娟,贾卫国,刘伟成,等.草莓灰霉病菌拮抗放线菌的筛选及活性测定[J].北方园艺, 2018, 42(4):59-65.
[13]陈宏州,庄义庆,杨敬辉.黄麻链霉菌NF0919菌株对草莓枯萎病的生防活性初探[J].江西农业学报, 2014, 26(11):54-57.
[14]吴翔,甘炳成,黄忠乾,等. 2株降解纤维素放线菌的鉴定[J].西南农业学报, 2014, 27(1):117-120.
[15]Adinarayana G, Venkateshan MR, Bapiraju VVSNK, et al. Cytotoxic compounds from the marine actinobacterium Streptomyces corchorusii, AUBN 1/7 1[J]. Russian Journal of Bioorganic Chemistry, 2006, 32(3):295-300.
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