硫氧化菌的吸附固定化及对硫化物的去除效果研究
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摘要
针对海水养殖环境中硫化物含量过高的问题,利用沸石及陶粒吸附固定化耐盐硫氧化菌嗜热氢弧菌(Hydrog-enovibrio thermophilus)TT,探讨其对还原性硫化物的去除能力,研究不同改性条件下陶粒对还原性硫化物的去除效果。研究发现,相较于沸石,陶粒由于表面疏松多孔,具有较强的微生物吸附能力;改性陶粒与未处理陶粒相比对硫化物的去除效果提高了35%左右,其中通过pH=8的氢氧化钠溶液改性的碱浸陶粒去除效果最好,对硫化物的去除率达到75%。结果表明,碱改性陶粒对该硫氧化菌具有较好的吸附固定能力,在海水养殖系统硫化物污染的持续控制和治理中具有很好的应用前景。
To remove sulfide in the sulfide-rich mariculture area,the adsorption ability of low-price zeolite and ceramsite to immobilize sulfur-oxidizing bacteria(Hydrogenovibrio thermophilus strain TT)was investigated by analyzing its sulfide removal rate.The modified method was explored in order to obtain higher sulfide removal ability.The results showed that ceramsite with a loose and porous structure had the better sulfide removal ability compared with zeolite.The sulfide removal rate of modified ceramsite increased to the maximum of 35%than natural material and cerasmite modified by alkali(pH=8)exhibited the best sulfide removal rate.In conclusion,the ceramsite modified by alkali showed better adsorption ability to sulfur-oxidizing bacteria,depicting aquite promising application for biological remediation of sulfide-rich mariculture system.
To remove sulfide in the sulfide-rich mariculture area,the adsorption ability of low-price zeolite and ceramsite to immobilize sulfur-oxidizing bacteria(Hydrogenovibrio thermophilus strain TT)was investigated by analyzing its sulfide removal rate.The modified method was explored in order to obtain higher sulfide removal ability.The results showed that ceramsite with a loose and porous structure had the better sulfide removal ability compared with zeolite.The sulfide removal rate of modified ceramsite increased to the maximum of 35%than natural material and cerasmite modified by alkali(pH=8)exhibited the best sulfide removal rate.In conclusion,the ceramsite modified by alkali showed better adsorption ability to sulfur-oxidizing bacteria,depicting aquite promising application for biological remediation of sulfide-rich mariculture system.
引文
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[27]Ma C,Qin D,Sun Q,et al.Removal of environmental estrogens by bacterial cell immobilization technique[J].Chemosphere,2016,144:607-614.
[28]Elliott C,Ye Z,Mojumdar S C,et al.A potential bacterial carrier for bioremediation[J].Journal of Thermal Analysis and Calorimetry,2007,90(3):707-711.
[29]彭昌盛,刘慧,张倩,等.颗粒粒径和水溶液性质对多孔介质渗透性的影响[J].中国海洋大学学报(自然科学版),2015,45(3):107-115.Peng C S,Liu H,Zhang Q,et al.Effects of particle size and solution properties on permeability of porous media[J].Periodical of Ocean University of China,2015,45(3):107-115.
[30]张桂芝,廖强,王永忠.微生物固定化载体材料研究进展[J].材料导报,2011,25(17):105-109.He X M,Ding L L,Zhang L L,et al.Performance,sludge characteristics and the microbial community dynamics of bulking sludge under different nitrogen and phosphorus imbalances[J].Environmental Science,2011,25(17):105-109.
[31]于凤娥,叶志平,郭杏妹,等.沸石的改性及其在废水处理中的应用[J].广东化工,2016,38(6):431-436.Yu F E,Ye Z P,Guo X M,et al.The method of modifying zeolite and their application in wastewater treatment[J].Guangdong Chemical Industry,2016,38(6):431-436.
[32]姜霞,周小宁,丁明玉,等.天然沸石及改性沸石去除低浓度氨氮的研究[J].环境科学研究,2008,21(5):37-42.Jiang X,Zhou X N,Ding M Y,et al.Study on low concentration ammonia nitrogen removal by natural and modified clinoptilolites[J].Research of Environmental Sciences,2008,21(5):37-42.
[33]姚丽群,高利平,托罗别克,等.活性炭的表面化学改性及其对有机硫化物的吸附性能的研究[J].燃料化学学报,2006,34(6):749-752.Yao L Q,Gao L P,Tuo L P K,et al.Chemicalmodification of activated carbon surface and its adsorption propertyof organic sulfur complexes[J].Journal of Fuel Chemistry and Technology,2006,34(6):749-752.
[34]Bayazidi P,Almasi H,Asl A K.Immobilization of lysozyme on bacterial cellulose nanofibers:Characteristics,antimicrobial activity and morphological properties[J].International Journal of Biological Macromolecules,2018,107:2544-2551.
[35]Lian Z,Ma Z,Wei J,et al.Preparation and characterization of immobilized lysozyme and evaluation of its application in edible coatings[J].Process Biochemistry,2012,47(2):201-208.
[36]梅佳军,张常青,刘嵘明,等.利用改性载体固定化大肠杆菌产琥珀酸[J].化工进展,201332(1):161-165.Mei J J,Zhang C Q,Liu R M,et al.Succinic acid production with immobilized cells of Escherichia coli onmodified carrier[J].Chemical Industry and Engineering Progress,2013,32(1):161-165.
[2]陈小红,陈宇锋,郑惠东,等.网箱养殖沉积环境中硫氧化菌的分离鉴定及生长特性[J].渔业研究,2016,38(6):431-436.Chen X H,Chen Y F,Zheng H D,et al.Isolation,identification and characteristics of a sulfide-oxidizing bacterium in sediment of marine[J].Journal of Fisheries Research,2016,38(6):431-436.
[3]Asaoka S,Yamamoto T,Yoshioka I,et al.Remediation of coastal marine sediments using granulated coal ash[J].Journal of Hazardous Materials,2009,172(1):92-98.
[4]张文革.南美白对虾养殖过程中硫化物的控制[J].河北渔业,2011(4):50-52.Zhang W G.The technology of sulfide control in the culture of Penaeus vannamei[J].Hebei Fisheries,2011(4):50-52.
[5]Sun J,Zhou J,Shang C,et al.Removal of aqueous hydrogen sulfide by granular ferric hydroxide-kinetics,capacity and reuse[J].Chemosphere,2014,117:324-329.
[6]杨娟,谢翼飞,李旭东,等.一株耐盐硫氧化细菌的分离鉴定及脱硫机理[J].应用与环境生物学报,2015(5):819-823.Yang J,Xie Y F,Li X D,et al.Isolation,identification and desulfurization mechanism of a sulfur-oxidizing bacterium with salt tolerance[J].Marine Environmental Science,2015(5):819-823.
[7]贡俊,张肇铭,王玉芬,等.一株高效脱硫菌的分离鉴定和脱硫特性研究[J].环境工程学报,2009,3(11):2031-2036.Gong J,Zhang Z M,Wang Y F,et al.Isolation,identification and desulfurization characteristics of an efficient desulfurization strain[J].Chinese Journal of Environmental Engineering,2009,3(11):2031-2036.
[8]Hou D,Shen X,Luo Q,et al.Enhancement of the diesel oil degradation ability of a marine bacterial strain by immobilization on a novel compound carrier material[J].Marine Pollution Bulletin,2013,67(1):146-151.
[9]Sekaran G,Karthikeyan S,Gupta V K,et al.Immobilization of Bacillus sp.in mesoporous activated carbon for degradation of sulphonated phenolic compound in wastewater[J].Materials Science and Engineering:C,2013,33(2):735-745.
[10]郑宇,王晓琼,赵阳国,等.包埋法固定化对硫氧化微生物菌群结构和功能的影响[J].微生物学报,2016,56(9):1504-1512.Zheng Y,Wang X Q,Zhao Y G,et al.Effects of immobilization on community structure and function ofsulfide oxidizing microbiota[J].Acta Microbiologica Sinica,2016,56(9):1504-1512.
[11]Ravichandra P,Gopal M,Annapurna J.Biological sulfide oxidation using autotrophic Thiobacillus sp.:Evaluation of different immobilization methods and bioreactors[J].Journal of Applied Microbiology,2009,106(4):1280-1291.
[12]邓晓皋.吸附法固定微生物细胞的研究进展[J].生物工程进展,1993,13(4):7-10.Deng X G.Research progress of immobilized microbial cells by adsorption method[J].Progress in Biotechnology,1993,13(4):7-10.
[13]Skoronski E,Souza D H,Ely C,et al.Immobilization of laccase fromAspergillus oryzae on graphene nanosheets[J].International Journal of Biological Macromolecules,2017,99:121-127.
[14]Vichuviwat R,Boonsombuti A,Luengnaruemitchai A,et al.Enhanced butanol production by immobilized Clostridium beijerinckii TISTR 1461using zeolite 13Xas a carrier[J].Bioresource Technology,2014,172:76-82.
[15]龚浩,郭劲松,方芳,等.改性陶粒对水中卡马西平去除的动态吸附实验及模型[J].环境工程学报,2016,10(7):3573-3579.Gong H,Guo J S,Fang F,et al.Experiments and model for dynamic adsorption of carbamazepine onto modified ceramisite in drinking water[J].Chinese Journal of Environmental Engineering,2016,10(7):3573-3579.
[16]Zhou W,Liu X,Dong X,et al.Sulfur-based autotrophic denitrification from the micro-polluted water[J].Journal of Environmental Sciences,2016,44:180-188.
[17]Rattanapan C,Boonsawang P,Kantachote D.Removal of H2S in down-flow GAC biofiltration using sulfide oxidizing bacteria from concentrated latex wastewater[J].Bioresource Technology,2009,100(1):125-130.
[18]Luo J,Tian G,Lin W.Enrichment,isolation and identification of sulfur-oxidizing bacteria from sulfide removing bioreactor[J].Journal of Environmental Sciences,2013,25(7):1393-1399.
[19]Pawlak Z,Pawlak A S.Modification of iodometric determination of total and reactive sulfide in environmental samples[J].Talanta,1999,48(2):347-353.
[20]Chung K,Okabe S.Continuous power generation and microbial community structure of the anode biofilms in a three-stage microbial fuel cell system[J].Applied Microbiology and Biotechnology,2009,83(5):965-977.
[21]Zhou J,Sui Z,Zhu J,et al.Characterization of surface oxygen complexes on carbon nanofibers by TPD,XPS and FT-IR[J].Carbon,2007,45(4):785-796.
[22]冯守帅,计云鹤,杨海麟.硫氧化菌种脱除硫化物生成单质硫限制性因素优化[J].微生物学通报,2016,43(1):36-43.Feng S S,Ji Y H,Yang H L.Optimization of restrictive factors during process of removing sulfide into elemental sulfur by sulfur oxidizer[J].Microbiology China,2016,43(1):36-43.
[23]Chen C,Wang A,Ren N,et al.Enhancing denitrifying sulfide removal with functional strains under micro-aerobic condition[J].Process Biochemistry,2010,45(6):1007-1010.
[24]Takai K,Hirayama H,Nakagawa T,et al.Thiomicrospira thermophila sp.nov.,a novel microaerobic,thermotolerant,sulfuroxidizing chemolithomixotroph isolated from a deep-sea hydrothermal fumarole in the TOTO caldera,Mariana Arc,Western Pacific[J].Int J Syst Evol Microbiol,2004,54(Pt6):2325-2333.
[25]Liu D,Sun J,Zhang J,et al.Response of the diatom flora in Jiaozhou Bay,China to environmental changes during the last century[J].Marine Micropaleontology,2008,66(3):279-290.
[26]王克明,许文友,庄树宏.固定化桔青霉气升式反应器生产核酸酶P1的研究[J].烟台大学学报(自然科学与工程版),2001,14(1):37-41.Wang K M,Xu W Y,Zhuang S H.Study on production of nuclease P1by immobilized penicilium citrinumcell in three phase airlift reactor[J].Journal of Yantai University(Natural Science and Engineering Edition),2001,14(1):37-41.
[27]Ma C,Qin D,Sun Q,et al.Removal of environmental estrogens by bacterial cell immobilization technique[J].Chemosphere,2016,144:607-614.
[28]Elliott C,Ye Z,Mojumdar S C,et al.A potential bacterial carrier for bioremediation[J].Journal of Thermal Analysis and Calorimetry,2007,90(3):707-711.
[29]彭昌盛,刘慧,张倩,等.颗粒粒径和水溶液性质对多孔介质渗透性的影响[J].中国海洋大学学报(自然科学版),2015,45(3):107-115.Peng C S,Liu H,Zhang Q,et al.Effects of particle size and solution properties on permeability of porous media[J].Periodical of Ocean University of China,2015,45(3):107-115.
[30]张桂芝,廖强,王永忠.微生物固定化载体材料研究进展[J].材料导报,2011,25(17):105-109.He X M,Ding L L,Zhang L L,et al.Performance,sludge characteristics and the microbial community dynamics of bulking sludge under different nitrogen and phosphorus imbalances[J].Environmental Science,2011,25(17):105-109.
[31]于凤娥,叶志平,郭杏妹,等.沸石的改性及其在废水处理中的应用[J].广东化工,2016,38(6):431-436.Yu F E,Ye Z P,Guo X M,et al.The method of modifying zeolite and their application in wastewater treatment[J].Guangdong Chemical Industry,2016,38(6):431-436.
[32]姜霞,周小宁,丁明玉,等.天然沸石及改性沸石去除低浓度氨氮的研究[J].环境科学研究,2008,21(5):37-42.Jiang X,Zhou X N,Ding M Y,et al.Study on low concentration ammonia nitrogen removal by natural and modified clinoptilolites[J].Research of Environmental Sciences,2008,21(5):37-42.
[33]姚丽群,高利平,托罗别克,等.活性炭的表面化学改性及其对有机硫化物的吸附性能的研究[J].燃料化学学报,2006,34(6):749-752.Yao L Q,Gao L P,Tuo L P K,et al.Chemicalmodification of activated carbon surface and its adsorption propertyof organic sulfur complexes[J].Journal of Fuel Chemistry and Technology,2006,34(6):749-752.
[34]Bayazidi P,Almasi H,Asl A K.Immobilization of lysozyme on bacterial cellulose nanofibers:Characteristics,antimicrobial activity and morphological properties[J].International Journal of Biological Macromolecules,2018,107:2544-2551.
[35]Lian Z,Ma Z,Wei J,et al.Preparation and characterization of immobilized lysozyme and evaluation of its application in edible coatings[J].Process Biochemistry,2012,47(2):201-208.
[36]梅佳军,张常青,刘嵘明,等.利用改性载体固定化大肠杆菌产琥珀酸[J].化工进展,201332(1):161-165.Mei J J,Zhang C Q,Liu R M,et al.Succinic acid production with immobilized cells of Escherichia coli onmodified carrier[J].Chemical Industry and Engineering Progress,2013,32(1):161-165.
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