高乌头炮制前后毒性部位多波长指纹图谱研究
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摘要
目的:建立高乌头炮制前后毒性部位多波长指纹图谱(MWCF),从物质基础上揭示高乌头炮制后毒性降低的原因。方法:通过HPLC梯度洗脱分别建立10批不同地区高乌头药材炮制前后正丁醇萃取部分多波长指纹图谱,采用"中药指纹图谱相似度评价系统2012版"进行分析。结果:建立了生、制高乌头正丁醇毒性部位多波长指纹图谱;在生、制品正丁醇毒性部位图谱中分别标出24个共有峰、17个共有峰,制品消失10个峰,新增7个峰,分别占生、制品共有峰总峰面积30.00%,26.38%;同时对比生、制品正丁醇萃取部位2个成分(高乌甲素、冉乌头碱)变化,发现炮制后高乌甲素含量降低至原来的1/27,冉乌头碱未检测到。结论:该方法重现性好、特征性强,可用于高乌头生、制品毒性部位的质量评价,为阐明高乌头炮制前后物质基础变化及炮制原理提供科学依据。
Objective: To set up an analysis method of multi-wavelength chromatographic fingerprints(MWCF) for toxicity parts from crude and processed the roots of Aconitum sinomontanum Nakai.(ASN), and to discuss the chemical composition changes after processing that reduce the toxicity. Methods: The MWCF of n-butanol extraction was prepared by HPLC gradient elution in 10 batches of different regions. And the Chinese Materia Medica(CMM) Fingerprint Similarity Evaluation System(2012 edition) was used for analysis. Results: The MWCF of n-butanol extract from crude and processed the roots of ASN was established. The gained 24 and 17 common peaks from crude and processed roots of ASN, respectively. The processed product disappeared by 10 peaks, accounting for 30.00% of the total peak area of the crude products; 7 new peaks were added, accounting for 26.38% of the total peak area of the processed products. At the same time, the changes of the two components(lappacontine and ranaconitine) in the extraction part of crude and processed n-butanol were compared. It was found that the content of lappaconitine decreased to 1/27 of the original, and ranaconitine was not detected. Conclusion: This method with good reproducibility and strong characteristic, could be used for the full quality evaluation of toxicity parts from crude and processed roots of ASN. To provide a scientific basis for elucidating the chemical substance base and processing principle of crude and processed roots of ASN.
Objective: To set up an analysis method of multi-wavelength chromatographic fingerprints(MWCF) for toxicity parts from crude and processed the roots of Aconitum sinomontanum Nakai.(ASN), and to discuss the chemical composition changes after processing that reduce the toxicity. Methods: The MWCF of n-butanol extraction was prepared by HPLC gradient elution in 10 batches of different regions. And the Chinese Materia Medica(CMM) Fingerprint Similarity Evaluation System(2012 edition) was used for analysis. Results: The MWCF of n-butanol extract from crude and processed the roots of ASN was established. The gained 24 and 17 common peaks from crude and processed roots of ASN, respectively. The processed product disappeared by 10 peaks, accounting for 30.00% of the total peak area of the crude products; 7 new peaks were added, accounting for 26.38% of the total peak area of the processed products. At the same time, the changes of the two components(lappacontine and ranaconitine) in the extraction part of crude and processed n-butanol were compared. It was found that the content of lappaconitine decreased to 1/27 of the original, and ranaconitine was not detected. Conclusion: This method with good reproducibility and strong characteristic, could be used for the full quality evaluation of toxicity parts from crude and processed roots of ASN. To provide a scientific basis for elucidating the chemical substance base and processing principle of crude and processed roots of ASN.
引文
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[4]谢斌,周军辉,石新卫,等.高乌头活性成分提取工艺的优化.植物学报,2014,49(2):198-202
[5]徐斌,张琴,郭夫江,等.高乌头的研究进展.中成药,2016,38(4):882-890
[6]李芸,胡昌江,侯桃霞,等.正交实验优选高乌头解毒的炮制工艺研究.中成药,2015,37(4):905-909
[7]张立军,戴海蓉,李芸,等.高乌头炮制品质量标准研究.中华中医药杂志,2018,33(2):675-678
[8]张立军,戴海蓉,李芸,等.高乌头炮制前后毒性部位与镇痛抗炎药效部位筛选研究.中药药理与临床,2017,33(4):78-82
[9]孙国祥,车磊,李闫飞.一种评价多波长中药色谱指纹图谱新方法——均谱法.中南药学,2011,9(7):533-538
[10]孙国祥,吴波,毕开顺.平行五波长高效液相色谱指纹图谱全息整合法定量鉴定杞菊地黄丸的整体质量.色谱,2010,28(9):877-884
[2]江苏新医学院.中药大辞典.下册.上海:上海科技出版社,2001:751-752
[3]Shrestha B B,Dall’Acqua S,Gewali M B,et al.New flavonoid glycosides from Aconitum naviculare(Brühl)Stapf,a medicinal herb from the trans-himalayan region of Nepal.Carbohydr Res,2006,341(12):2161-2165
[4]谢斌,周军辉,石新卫,等.高乌头活性成分提取工艺的优化.植物学报,2014,49(2):198-202
[5]徐斌,张琴,郭夫江,等.高乌头的研究进展.中成药,2016,38(4):882-890
[6]李芸,胡昌江,侯桃霞,等.正交实验优选高乌头解毒的炮制工艺研究.中成药,2015,37(4):905-909
[7]张立军,戴海蓉,李芸,等.高乌头炮制品质量标准研究.中华中医药杂志,2018,33(2):675-678
[8]张立军,戴海蓉,李芸,等.高乌头炮制前后毒性部位与镇痛抗炎药效部位筛选研究.中药药理与临床,2017,33(4):78-82
[9]孙国祥,车磊,李闫飞.一种评价多波长中药色谱指纹图谱新方法——均谱法.中南药学,2011,9(7):533-538
[10]孙国祥,吴波,毕开顺.平行五波长高效液相色谱指纹图谱全息整合法定量鉴定杞菊地黄丸的整体质量.色谱,2010,28(9):877-884
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