不同产地巫山淫羊藿指纹图谱的主成分、因子和聚类分析
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摘要
目的:采用HPLC建立巫山淫羊藿的指纹图谱,结合主成分、因子和聚类分析对不同产地巫山淫羊藿综合品质进行研究,为其质量评价提供理论基础。方法:采用Agilent Infinity Lab Poroshe1l 120 SB-C18色谱柱(3. 0 mm×100 mm,2. 7μm),流动相乙腈(A)-水(B)梯度洗脱(0～5 min,25%～26%A; 5～6 min,26%～34%A; 6～11 min,34%～38. 5%A; 11～17 min,38. 5%～100%A; 17～20 min,100%A),流速0. 8 m L·min~(-1),检测波长270 nm,柱温30℃。结果:聚类分析较好地对不同产地的巫山淫羊藿进行分类,贵州产巫山淫羊藿和重庆巫山淫羊藿在聚类分析中明显分为二类,相距较远,说明由于环境气候的影响,不同产地的淫羊藿在品质上存在差异。主成分、因子分析结果,重庆巫山淫羊藿质量总体优于贵州产巫山淫羊藿,其中CQWS-02(重庆市巫山县官阳镇鸦鹊村),CQWS-10(重庆市巫山县官阳镇河湾)两个产地的样品在优质品种筛选时可重点考虑。另外,1号(朝藿定A),2号(朝藿定B),4号(淫羊藿苷),5号(未知成分)共有峰可作为巫山淫羊藿质量评价指标成分。结论:通过主成分、因子和聚类分析,可实现快速分析,发挥各自优势,互相验证和补充,多方面综合评判不同产地巫山淫羊藿药材的质量。
Objective: To establish the fingerprint of Epimedium wushanense by HPLC,and study the comprehensive quality of E. wushanense by combining principal components,factors and cluster analysis,so as to provide theoretical basis for its quality evaluation. Method: The chromatographic column was Agilent infinitylab poroshell 120 SB-C_(18)( 3. 0 mm × 100 mm,2. 7 μm),the flow phase was acetonitrile( A)-water( B) with a gradient of 0-5 min,25%-26% A; 5-6 min,26%-34% A; 6-11 min,34%-38. 5% A; 11-17 min,38. 5%-100% A; 17-20 min,100% A,the flow rate was 0. 8 m L·min~(-1),the detection wavelength was 270 nm,and the column temperature was 30 ℃. Result: The cluster analysis better classified E. wushanense from different producing areas. E. wushanense from Guizhou province and E. wushanense from Chongqing were classified as classⅡ. E. wushanense from Guizhou province and Chongqing were far apart, indicating that the quality of E. wushanense varies from place to place affected by environment and climate. The results of principal component analysis showed that the quality of E. wushanense produced in Chongqing was better than that of E. wushanense produced in Guizhou. Among them,CQWS-02( Yaque village,Guanyang town,Wushan county,Chongqing)and CQWS-10( Hewan,Guanyang town,Wushan county,Chongqing) can be considered in the selection of highquality varieties. In addition,No. 1 common peak( epimedin A),No. 2 common peak( epimedin B),No. 4 common peak( icariin) and No. 5 common peak( unknown component) in the fingerprint of the test samples could be used as the evaluation index components of E. wushanense quality. Conclusion: Principal components,factors and cluster analysis are used to achieve the rapid analysis,and their respective advantages are brought into full play for mutual verification and supplement. And the quality of E. wushanense in different origins can be comprehensively evaluated in all-round ways.
Objective: To establish the fingerprint of Epimedium wushanense by HPLC,and study the comprehensive quality of E. wushanense by combining principal components,factors and cluster analysis,so as to provide theoretical basis for its quality evaluation. Method: The chromatographic column was Agilent infinitylab poroshell 120 SB-C_(18)( 3. 0 mm × 100 mm,2. 7 μm),the flow phase was acetonitrile( A)-water( B) with a gradient of 0-5 min,25%-26% A; 5-6 min,26%-34% A; 6-11 min,34%-38. 5% A; 11-17 min,38. 5%-100% A; 17-20 min,100% A,the flow rate was 0. 8 m L·min~(-1),the detection wavelength was 270 nm,and the column temperature was 30 ℃. Result: The cluster analysis better classified E. wushanense from different producing areas. E. wushanense from Guizhou province and E. wushanense from Chongqing were classified as classⅡ. E. wushanense from Guizhou province and Chongqing were far apart, indicating that the quality of E. wushanense varies from place to place affected by environment and climate. The results of principal component analysis showed that the quality of E. wushanense produced in Chongqing was better than that of E. wushanense produced in Guizhou. Among them,CQWS-02( Yaque village,Guanyang town,Wushan county,Chongqing)and CQWS-10( Hewan,Guanyang town,Wushan county,Chongqing) can be considered in the selection of highquality varieties. In addition,No. 1 common peak( epimedin A),No. 2 common peak( epimedin B),No. 4 common peak( icariin) and No. 5 common peak( unknown component) in the fingerprint of the test samples could be used as the evaluation index components of E. wushanense quality. Conclusion: Principal components,factors and cluster analysis are used to achieve the rapid analysis,and their respective advantages are brought into full play for mutual verification and supplement. And the quality of E. wushanense in different origins can be comprehensively evaluated in all-round ways.
引文
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[11]聂韡,朱培林,房海灵,等. HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[12]刘威,龚伟,张嵩,等.基于多元统计分析的鹿茸品质评价[J].中国实验方剂学杂志,2018,24(14):57-62.
[13]丁捷,赵雪梅,朱金艳,等.基于主成分、因子和聚类分析淡水鱼品种对速冻青稞鱼面品质的影响[J].食品工业科技,2018,39(1):34-40,51.
[14]张晓冬,陈龙,白玉,等.近红外光谱结合主成分分析和聚类分析鉴别炉甘石生品、伪品和炮制品[J].中国实验方剂学杂志,2018,24(15):1-8.
[15]陈懿瑶,罗阳,闫燊,等. HPAEC-PAD法测定石斛属植物单糖组分与主成分聚类分析[J].中国实验方剂学杂志,2018,24(10):106-111.
[16]石召华,叶利春,关小羽,等.娑罗子药材HPLC色谱指纹图谱的建立及其在药材鉴定中的应用[J].中国实验方剂学杂志,2018,24(14):52-56.
[17]杨雯.中国淫羊藿属药用植物孢粉学及其药材品质评价研究[D].贵阳:贵阳中医学院,2015.
[18]蔡晓洋,张思荻,曾俊,等.基于主成分分析和聚类分析的栀子种质资源评价[J].中国实验方剂学杂志,2017,23(14):30-37.
[19]公丽艳,孟宪军,刘乃侨,等.基于主成分与聚类分析的苹果加工品质评价[J].农业工程学报,2014,30(13):276-285.
[20]谈小刚.广东省旅游经济发展的区域差异及其影响因素分析[D].广州:广州大学,2013.
[21]黄娇.指纹图谱和主成分分析法评价不同海拔高度的桔梗药材质量[J].食品工业科技,2015,36(13):309-313,320.
[22]宋江峰,刘春泉,姜晓青,等.基于主成分与聚类分析的菜用大豆品质综合评价[J].食品科学,2015,36(13):12-17.
[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:167.
[3]张华峰,杨晓华.淫羊藿药材质量控制的问题与对策[J].中草药,2009,40(1):160-163.
[4] WANG C C,SU J Y,CAI J Y,et al. Response surface analysis for the optimization of extraction condition for polysaccharides from Epimedium polysaccharides and studies on its tumor immune activities[J]. Acta Pharm Sin,2016,51(9):1464-1471.
[5] WANG C C,FENG L,SU J Y,et al. Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice[J]. J Ethnopharmacol,2017,207(31):8-18.
[6]孙勇.淫羊藿苷对髓母细胞瘤细胞生长的作用及其机制研究[D].济南:山东大学,2017.
[7] ZHOU J,MA Y H,ZHOU Z,et al. Intestinal absorption and metabolism of Epimedium flavonoids in osteoporosis rats[J]. Drug Metab Dispos,2015,43(10):1590-1600.
[8]权秋梅,黎云祥.巫山淫羊藿开花物候与生殖特征[J].生态学杂志,2013,32(4):859-866.
[9]李作洲,徐艳琴,王瑛,等.淫羊藿属药用植物的研究现状与展望[J].中草药,2005,36(2):289-295.
[10]刘仁权. SPSS统计软件[M].北京:中国中医药出版社,2007:150.
[11]聂韡,朱培林,房海灵,等. HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[12]刘威,龚伟,张嵩,等.基于多元统计分析的鹿茸品质评价[J].中国实验方剂学杂志,2018,24(14):57-62.
[13]丁捷,赵雪梅,朱金艳,等.基于主成分、因子和聚类分析淡水鱼品种对速冻青稞鱼面品质的影响[J].食品工业科技,2018,39(1):34-40,51.
[14]张晓冬,陈龙,白玉,等.近红外光谱结合主成分分析和聚类分析鉴别炉甘石生品、伪品和炮制品[J].中国实验方剂学杂志,2018,24(15):1-8.
[15]陈懿瑶,罗阳,闫燊,等. HPAEC-PAD法测定石斛属植物单糖组分与主成分聚类分析[J].中国实验方剂学杂志,2018,24(10):106-111.
[16]石召华,叶利春,关小羽,等.娑罗子药材HPLC色谱指纹图谱的建立及其在药材鉴定中的应用[J].中国实验方剂学杂志,2018,24(14):52-56.
[17]杨雯.中国淫羊藿属药用植物孢粉学及其药材品质评价研究[D].贵阳:贵阳中医学院,2015.
[18]蔡晓洋,张思荻,曾俊,等.基于主成分分析和聚类分析的栀子种质资源评价[J].中国实验方剂学杂志,2017,23(14):30-37.
[19]公丽艳,孟宪军,刘乃侨,等.基于主成分与聚类分析的苹果加工品质评价[J].农业工程学报,2014,30(13):276-285.
[20]谈小刚.广东省旅游经济发展的区域差异及其影响因素分析[D].广州:广州大学,2013.
[21]黄娇.指纹图谱和主成分分析法评价不同海拔高度的桔梗药材质量[J].食品工业科技,2015,36(13):309-313,320.
[22]宋江峰,刘春泉,姜晓青,等.基于主成分与聚类分析的菜用大豆品质综合评价[J].食品科学,2015,36(13):12-17.
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