广西壮族绝经后妇女雌激素受体-α基因多态性对碱性磷酸酶和骨密度的影响
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摘要
目的明确雌激素受体(ER)-α不同基因型在广西壮族妇女中分布,了解其不同基因与广西壮族绝经后妇女血清碱性磷酸酶(ALP)水平和跟骨骨密度(BMD)的关系。方法对612例广西壮族绝经妇女采外周静脉血5 ml,提取DNA检测ER-α基因,用试剂盒法测定相应标本的血清ALP。用超声BMD仪检测受试者右侧跟骨BMD。结果 60岁之前绝经妇女ER-α所有基因型的ALP和BMD都是随着年龄增大而降低的,各基因型的同一年龄组之间的ALP和BMD均无明显差别(P>0. 05)。而60岁后ER-αPvuⅡ酶切的P等位基因与同一年龄组其他4个基因组比较,ALP明显升高(P<0. 05),而BMD明显降低(P<0. 05); 65岁后ER-αXbaⅠ酶切的Xx等位基因与同一年龄组其他4个基因组比较,ALP明显降低,而BMD明显提高(P<0. 05)。结论 ER-α的P等位基因是绝经后骨质疏松易感基因,此类基因型绝经妇女应该提前防治;而ER-α的Xx等位基因的BMD较高,不易患绝经后骨质疏松。应该根据不同基因型个体采取个性化防治。
Objective To determine the distribution of different oestrogen receptor-beta genes in women of Guangxi Zhuang nationality and understand the relationship between different genes and serum alkaline phosphatase level and bone mineral density ( BMD) in postmenopausal women of Guangxi Zhuang nationality.Methods 5 ml peripheral venous blood was collected from 612 post-menopausal women in Guangxi Zhuang nationality,DNA was extracted to detect the estrogen receptor-sterile gene. The BMD of the right calcaneal was measured by ultrasonic bone density meter. Results Before the age of 60,alkaline phosphatase and BMD of all estrogen receptor-alpha genotypes in postmenopausal women decreased with aging and there was no significant difference between alkaline phosphatase and BMD in the same age group of each genotype( P>0.05). And estrogen receptor after 60 years of age-alpha Pvu Ⅱ enzyme P allele compared with the same age group of four other genomes,alkaline phosphatase increased significantly( P<0.05) and BMD decreased obviously( P< 0.05). After the 65-year-old estrogen receptor-alpha Xba Ⅰ enzyme Xx alleles and other four genome in the same age groups,alkaline phosphatase decreased obviously and BMD increased significantly( P<0.05).Conclusions The P allele of estrogen receptor-positive is a predisposing gene of postmenopausal osteoporosis,which is more susceptible to postmenopausal osteoporosis. The Xx allele of estrogen receptor( alpha) has higher BMD and is less susceptible to postmenopausal osteoporosis.
Objective To determine the distribution of different oestrogen receptor-beta genes in women of Guangxi Zhuang nationality and understand the relationship between different genes and serum alkaline phosphatase level and bone mineral density ( BMD) in postmenopausal women of Guangxi Zhuang nationality.Methods 5 ml peripheral venous blood was collected from 612 post-menopausal women in Guangxi Zhuang nationality,DNA was extracted to detect the estrogen receptor-sterile gene. The BMD of the right calcaneal was measured by ultrasonic bone density meter. Results Before the age of 60,alkaline phosphatase and BMD of all estrogen receptor-alpha genotypes in postmenopausal women decreased with aging and there was no significant difference between alkaline phosphatase and BMD in the same age group of each genotype( P>0.05). And estrogen receptor after 60 years of age-alpha Pvu Ⅱ enzyme P allele compared with the same age group of four other genomes,alkaline phosphatase increased significantly( P<0.05) and BMD decreased obviously( P< 0.05). After the 65-year-old estrogen receptor-alpha Xba Ⅰ enzyme Xx alleles and other four genome in the same age groups,alkaline phosphatase decreased obviously and BMD increased significantly( P<0.05).Conclusions The P allele of estrogen receptor-positive is a predisposing gene of postmenopausal osteoporosis,which is more susceptible to postmenopausal osteoporosis. The Xx allele of estrogen receptor( alpha) has higher BMD and is less susceptible to postmenopausal osteoporosis.
引文
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9杨小猛,陈丽娟,李宝花,等.深圳市学龄前儿童血清碱性磷酸酶参考值范围的建立及临床意义[J].中国妇幼保健,2011;26(8):1227-8.
10李海,陈建海,王金花,等.山茶籽联合雌二醇对去卵巢大鼠骨组织和骨代谢生化指标的影响[J].中国妇幼保健,2012;27(4):595-8.
2 Luo Z,Liu M,Sun L,et al.Icariin recovers the osteogenic differentiation and bone formation of bone marrow stromal cells from a rat model of estrogen deficiency-induced osteoporosis[J].Mol Med Rep,2015;12(1):382-8.
3 Shoukry A,Shalaby SM,Etewa RL,et al.Association of estrogen receptorβand estrogen-related receptorαgene polymorphisms with bone mineral density in postmenopausal women[J].Mol Cell Biochem,2015;45(2):23-31.
4 Deng W,Han JC,Chen L,et al.Estrogen receptor alpha gene PvuⅡpolymorphism and risk of fracture in postmenopausal women:a metaanalysis[J].Genet Mol Res,2015;14(1):1293-300.
5 Tural S,Kara N,Alayli G,et al.Association between osteoporosis and polymorphisms of the bone Gla protein,estrogen receptor 1,collagen1-A1 and calcitonin receptor genes in Turkish postmenopausal women[J].Gene,2013;515(1):167-72.
6 Obemayer PB,Chararas C,Kotschan S,et al.Genetic background of osteoporosis[J].Acta Med Austriaca,2000;27(1):18.
7 Schuit SC,de Jong FH,Stolk L,et al.Estrogen receptor alphu gene polymorphisms are associated with estradiol levels in postmenopausal women[J].Eur J Endocrinol,2005;153(2):327-34.
8 Luo D,Liu Y,Zhou Y,et al.Association between dietary phytoestrogen intake and bone mineral density varied with estrogen receptor alpha gene polymorphisms in southern Chinese postmenopausal women[J].Food Funct,2015;6(6):1977-83.
9杨小猛,陈丽娟,李宝花,等.深圳市学龄前儿童血清碱性磷酸酶参考值范围的建立及临床意义[J].中国妇幼保健,2011;26(8):1227-8.
10李海,陈建海,王金花,等.山茶籽联合雌二醇对去卵巢大鼠骨组织和骨代谢生化指标的影响[J].中国妇幼保健,2012;27(4):595-8.
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