基于信息熵赋权法的正交试验优化七味蟾参方提取工艺研究
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摘要
目的:优化七味蟾参方的水提取工艺。方法:采用紫外法测定壁虎和西洋参中的多糖含量,高效液相色谱(HPLC)法测定喜树果中喜树碱、西洋参中人参皂苷Rb_1的含量;以上述3种成分的提取率及干膏得率、HPLC指纹图谱相似度作为评价指标,通过信息熵赋权法确定各指标权重而计算综合评分;以煎煮时间、加水量、煎煮次数为因素,设计L_9(3~4)正交试验筛选七味蟾参方的最优提取工艺并进行验证试验。结果:最优提取工艺为药材加10倍量水,煎煮3次,每次煎煮1.0 h。验证试验结果显示,在3次提取试验中,多糖、喜树碱、人参皂苷Rb_1平均提取率分别为74.306%、13.860%、52.958%,干膏平均得率为16.150%,指纹图谱相似度平均值为0.991,各指标RSD均小于3.0%(n=3)。结论:优化后的提取工艺重复性好、稳定可行,为该处方的后续开发和产业化生产提供参考依据。
OBJECTIVE:To optimize the water extraction technology of Qiwei chanshen formula. METHODS:The content ofpolysaccharides from Gekko japonicus and Panax quinquefolium was determined by UV spectrophotometry, the contents ofcamptothecin from Camptotheca acuminata and ginsenoside Rb_1 from P. quinquefolium were determined by HPLC. The extractionrate of above three components, dry extractum yield and HPLC fingerprint similarity were used as evaluation indicators;information entropy theory was used to determine the weight of each indicator so as to calculate comprehensive score. L_9(3~4)orthogonal test was used to screen the optimal extraction technology of Qiwei chanshen formula with decoction time,water volumeand decoction times as factors. Validation test was also performed. RESULTS:The optimal extraction technology included 10-foldwater, decocting for 3 times, 1.0 h each time. The results of validation test showed that the average extraction rate ofpolysaccharide,camptothecin and ginsenoside Rb_1 were 74.306%,13.860% and 52.958%,respectively. The average dry extractumyield was 16.150%,the average value of fingerprint similarity was 0.991(all RSDs<3.0%,n=3). CONCLUSIONS:Theoptimized extraction technology is reproducible,stable and feasible,providing reference for the subsequent development andindustrial production of the formula.
OBJECTIVE:To optimize the water extraction technology of Qiwei chanshen formula. METHODS:The content ofpolysaccharides from Gekko japonicus and Panax quinquefolium was determined by UV spectrophotometry, the contents ofcamptothecin from Camptotheca acuminata and ginsenoside Rb_1 from P. quinquefolium were determined by HPLC. The extractionrate of above three components, dry extractum yield and HPLC fingerprint similarity were used as evaluation indicators;information entropy theory was used to determine the weight of each indicator so as to calculate comprehensive score. L_9(3~4)orthogonal test was used to screen the optimal extraction technology of Qiwei chanshen formula with decoction time,water volumeand decoction times as factors. Validation test was also performed. RESULTS:The optimal extraction technology included 10-foldwater, decocting for 3 times, 1.0 h each time. The results of validation test showed that the average extraction rate ofpolysaccharide,camptothecin and ginsenoside Rb_1 were 74.306%,13.860% and 52.958%,respectively. The average dry extractumyield was 16.150%,the average value of fingerprint similarity was 0.991(all RSDs<3.0%,n=3). CONCLUSIONS:Theoptimized extraction technology is reproducible,stable and feasible,providing reference for the subsequent development andindustrial production of the formula.
引文
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[17]于晓娜,崔波,任贵兴.西洋参多糖的研究进展[J].食品科学,2014,35(9):301-305.
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[19]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017,48(2):278-282.
[20]张东,张宁.物理学中的熵理论及其应用研究[J].北京联合大学学报,2007,21(1):4-8.
[21]吴璐,杨华生.基于信息熵理论的中药提取工艺优选[J].中国实验方剂学杂志,2012,18(9):29-31.
[2]彭红英,江涛,刘敏珊,等.正交试验优化益骨颗粒的提取工艺[J].中国药房,2017,28(19):2684-2687.
[3]任烨,李厚聪,刘永恒,等.加拿大原产地西洋参多糖提取工艺优化研究[J].西南民族大学学报(自然科学版),2011,37(6):940-945.
[4]陈军辉,谢明勇,易秀琴,等.西洋参多糖提取新工艺研究[J].时珍国医国药,2005,16(10):977-979.
[5]隆林,罗朝凤,李天先,等.喜树果质量标准修定研究[J].现代食品,2017,15(30):87-92.
[6]王振涛,常红波,黄海英,等.HPLC法测定抗纤益心浓缩丸中人参皂苷Rg1、Rb1、Re的含量[J].中医临床研究,2016,8(28):24-27.
[7]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:131.
[8]谢凡,施崇精,杨秀清,等.基于信息熵理论的正交设计结合指纹图谱优选加味四妙颗粒的提取工艺[J].中草药,2018,49(6):1331-1337.
[9]李国转,于凡,陈卫东,等.正交试验结合HPLC指纹图谱优选丹参总酚酸的提取工艺[J].中药材,2017,40(5):1159-1162.
[10]王丹,程刚,许汉林,等.丹参提取工艺的指纹图谱技术结合正交试验优化[J].时珍国医国药,2016,27(4):857-860.
[11]李淼,王星星,康小东,等.基于信息熵理论的哮喘方水提取工艺优选研究[J].中草药,2016,47(12):2113-2117.
[12]王仁杰,李淼,闫明,等.信息熵理论在热毒宁口服制剂中金银花与栀子提取工艺优选中的应用[J].中草药,2015,46(5):683-687.
[13]孙超,孙明江.壁虎抗肿瘤作用的研究进展[J].山东中医药大学学报,2011,35(6):576-578.
[14]WU XZ,CHEN D,XIE GR.Effects of Gekko sulfated polysaccharide on the proliferation and differentiation of hepatic cancer cell line[J].Cell Biol Int,2006,30(8):659-664.
[15]代鲁平,宋春霞,邵先祥,等.抗癌药物喜树碱类衍生物的研究进展[J].中国药学杂志,2010,45(23):1813-1815.
[16]喻春皓,王冲之,袁钧苏.西洋参抗癌研究进展:以结直肠癌为研究范例[J].药学学报,2013,48(7):986-992.
[17]于晓娜,崔波,任贵兴.西洋参多糖的研究进展[J].食品科学,2014,35(9):301-305.
[18]伍振峰,陈伟良,王雅琪,等.丹参减压提取工艺优化及技术适宜性研究[J].中草药,2014,45(6):795-800.
[19]汪露露,何丹丹,王满,等.基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究[J].中草药,2017,48(2):278-282.
[20]张东,张宁.物理学中的熵理论及其应用研究[J].北京联合大学学报,2007,21(1):4-8.
[21]吴璐,杨华生.基于信息熵理论的中药提取工艺优选[J].中国实验方剂学杂志,2012,18(9):29-31.
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