珍珠龙胆石斑鱼听觉阈值研究
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摘要
珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)是重要的海水养殖经济鱼类,通过建立稳定的珍珠龙胆石斑鱼听性脑干反应(auditory brainstem response,ABR)检测系统,分别施加100、200、300、500、800、1 000、1 500、2 000、3 000、5 000和10 000 Hz的正弦波声音刺激信号,并应用该系统检测20条体长为32～35 cm的珍珠龙胆石斑鱼的听觉阈值。结果显示:该听性脑干反应检测系统获得的响应为听觉电生理反应,所有珍珠龙胆石斑鱼的听频范围为100～5 000 Hz,其中最适听频范围为100～2 000 Hz,最敏感声音频率为300 Hz,听觉阈值约为75. 8 d B。根据得到的平均听觉阈值,构建了珍珠龙胆石斑鱼的听性脑干反应听力曲线。研究表明:研究获得的珍珠龙胆石斑鱼听觉阈值及曲线对于研究噪声对不同养殖模式下石斑鱼的动物福利具有重要意义,不仅是评估噪声对石斑鱼影响的直接试验数据,也能为养殖环境的优化提供间接参考。
Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ is predominantly a commercial species cultured in seawater. An stable auditory brainstem response( ABR) detection system in Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ was established and sine wave acoustic stimulus signals of 100,200,300,500,800,1 000,1 500,2 000,3 000,5 000 and 10 000 Hz were applied to test the auditory thresholds of 20 Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂ with body length of 32-35 cm. The results showed that the response to the brainstem potential detection system was auditory electrophysiological response,and the auditory frequency range of all Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂ was100-5 000 Hz,where the best hearing was observed at 100-2 000 Hz,and the most sensitive sound frequency was 300 Hz with threshold of 75. 8 d B. ABR audiogram of Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ was created based on the average auditory thresholds obtained. The study showed that the auditory thresholds and the audiogram of Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ obtained have an important significance in studying effects of noise on animal welfare of Epinephelus in different aquaculture mode,and they are not only the direct data for evaluating the effect of noise on Epinephelus,but also can provide indirect reference for optimization of aquaculture environment.
Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ is predominantly a commercial species cultured in seawater. An stable auditory brainstem response( ABR) detection system in Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ was established and sine wave acoustic stimulus signals of 100,200,300,500,800,1 000,1 500,2 000,3 000,5 000 and 10 000 Hz were applied to test the auditory thresholds of 20 Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂ with body length of 32-35 cm. The results showed that the response to the brainstem potential detection system was auditory electrophysiological response,and the auditory frequency range of all Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂ was100-5 000 Hz,where the best hearing was observed at 100-2 000 Hz,and the most sensitive sound frequency was 300 Hz with threshold of 75. 8 d B. ABR audiogram of Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus ♂ was created based on the average auditory thresholds obtained. The study showed that the auditory thresholds and the audiogram of Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ obtained have an important significance in studying effects of noise on animal welfare of Epinephelus in different aquaculture mode,and they are not only the direct data for evaluating the effect of noise on Epinephelus,but also can provide indirect reference for optimization of aquaculture environment.
引文
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[13]YANG Y S,SEO D O,LEE C H. A basic study for the behaviourcontrol of sevenband grouper,Epinephelus septemfasciatus,tounderwater low frequency sound I. The Auditory thresholds ofsevenband grouper[J]. Journal of Korea Information&Communications Society,1999,35(4):391-396.
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[15]LADICH F. Did auditory sensitivity and vocalization evolveindependently in otophysan fishes[J]. Brain,Behavior andEvolution,1999,53(5/6):288-304.
[16]MANN D,HIGGS D M,TAVOLGA W N,et al. Ultrasounddetection by clupeiform fishes[J]. The Journal of the AcousticalSociety of America,2001,109(6):3048-3054.
[17]心砚.鱼类的听觉[J].垂钓,2002(6):55-56.
[18]LOVELL J M,FINDLAY M M,NEDWELL J R,et al,Thehearing abilities of the silver carp(Hypopthalmichthys molitrix)and bighead carp(Aristichthys nobilis)[J]. Comp BiochemPhys A,2006,143(3):286-291.
[19]MANN D,LU Z,POPPER A. A clupeid fish can detectultrasound[J]. Nature,1997,389:341.
[20]邢彬彬,王振宇,张国胜,等.基于心电图法(ECG)的牙鲆听觉特性研究[J].中国水产科学,2018(3):467-474.
[21]CODARIN A,WYSOCKI L E,LADICH F,et al. Effects of ambientand boat noise on hearing and communication in three fish speciesliving in a marine protected area(Miramare,Italy)[J]. Mar PollutBull,2009,58(12):1880-1887.
[22]YAN H,FINE M,HORN N,et al. Variability in the role of thegasbladder in fish audition[J]. Journal of ComparativePhysiology A,2000,186(5):435-445.
[23]CORDOVA M S,BRAUN C B. The use of anesthesia duringevoked potential audiometry in goldfish(Carassius auratus)[J].Brain Res,2007,1153(1):78-83.
[2]宋红桥,王振华,单建军,等.人为噪音对鱼类影响的研究进展[J].农学学报,2017(11):46-50.
[3]刘猛,危起伟,杜浩,等.胭脂鱼听觉阈值研究[J].中国水产科学,2013(4):750-757.
[4] KENYON T,LADICH F,YAN H. A comparative study of hearingability in fishes:the auditory brainstem response approach[J].Comp Physiol A,1998,182(3):307-318.
[5] LADICH F,WYSOCKI L E. Does speaker presentation affectauditory evoked potential thresholds in goldfish[J]. CompBiochem Phys A,2009,154(3):341-346.
[6]潘豪来,王际平,于栋帧,等.斑马鱼听觉诱发电位检测系统的建立和应用[J].听力学及言语疾病杂志,2017(3):269-274.
[7]张国胜,傅恩波,许传才,等.鱼类的听觉特性[A]//中国水产学会学术年会论文集[C].北京:海洋出版社,2000:413-419.
[8]张阳,戴媛媛,吴宇钢,等.心电图技术和听性脑干反应技术在测定鱼类听觉性能上的比较[J].河北渔业,2009(5):51-55.
[9] JEWETT D L. Volume-conducted potentials in response toauditory stimuli as detected by averaging in the cat[J].Electroencephalography and clinical neurophysiology,1970,28(6):609-618.
[10]JEWETT D L,WILLISTON J S. Auditory-evoked far fieldsaveraged from the scalp of humans[J]. Brain,1971,94(4):681-696.
[11]LECHNER W,WYSOCKI L E,LADICH F. Ontogeneticdevelopment of auditory sensitivity and sound production in thesqueaker catfish Synodontis schoutedeni[J]. BMC Biology,2010,8(1):1-12.
[12]张旭光,郭弘艺,宋佳坤.褐菖鲉的听觉阈值研究[J].水生生物学报,2018(3):593-598.
[13]YANG Y S,SEO D O,LEE C H. A basic study for the behaviourcontrol of sevenband grouper,Epinephelus septemfasciatus,tounderwater low frequency sound I. The Auditory thresholds ofsevenband grouper[J]. Journal of Korea Information&Communications Society,1999,35(4):391-396.
[14]TAKAHITO K,HIROSUKE I,TOMOYOKI K,et al. Measurementsof auditory sensitivity in common carp Cyprinus carpio by theauditory brainstem response technique and cardiac conditioningmethod[J]. Fisheries Science,2005,71(1):95-100.
[15]LADICH F. Did auditory sensitivity and vocalization evolveindependently in otophysan fishes[J]. Brain,Behavior andEvolution,1999,53(5/6):288-304.
[16]MANN D,HIGGS D M,TAVOLGA W N,et al. Ultrasounddetection by clupeiform fishes[J]. The Journal of the AcousticalSociety of America,2001,109(6):3048-3054.
[17]心砚.鱼类的听觉[J].垂钓,2002(6):55-56.
[18]LOVELL J M,FINDLAY M M,NEDWELL J R,et al,Thehearing abilities of the silver carp(Hypopthalmichthys molitrix)and bighead carp(Aristichthys nobilis)[J]. Comp BiochemPhys A,2006,143(3):286-291.
[19]MANN D,LU Z,POPPER A. A clupeid fish can detectultrasound[J]. Nature,1997,389:341.
[20]邢彬彬,王振宇,张国胜,等.基于心电图法(ECG)的牙鲆听觉特性研究[J].中国水产科学,2018(3):467-474.
[21]CODARIN A,WYSOCKI L E,LADICH F,et al. Effects of ambientand boat noise on hearing and communication in three fish speciesliving in a marine protected area(Miramare,Italy)[J]. Mar PollutBull,2009,58(12):1880-1887.
[22]YAN H,FINE M,HORN N,et al. Variability in the role of thegasbladder in fish audition[J]. Journal of ComparativePhysiology A,2000,186(5):435-445.
[23]CORDOVA M S,BRAUN C B. The use of anesthesia duringevoked potential audiometry in goldfish(Carassius auratus)[J].Brain Res,2007,1153(1):78-83.