葡萄糖氧化酶和过氧化氢酶对肉鸡生长性能及养分代谢率的影响
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摘要
试验以玉米豆粕为基础日粮,在日粮中添加不同水平的葡萄糖氧化酶和过氧化氢酶,探讨了两种酶单独或者复合后替代抗生素对肉鸡生长性能及养分代谢率的影响。试验选用1 080只1日龄健康的肉仔鸡,公母各半,按照试验要求随机分为9组,即对照组,抗生素组,处理组Ⅰ、Ⅱ组和处理Ⅲ组均加葡萄糖氧化酶150 g/t,活力分别是1 000、2 000 U/g和4 000 U/g,处理Ⅳ、Ⅴ组和处理Ⅵ组均加过氧化氢酶150 g/t,活力分别是5 000、10 000、20 000 U/g,处理Ⅶ组复合酶150 g/t(葡萄糖氧化酶2 000 U/g、过氧化氢酶10 000 U/g)。结果表明,从料重比来看,与对照组和抗生素组相比,1～21 d各试验组显著降低(P<0.05),22～42 d除了处理Ⅱ组和处理Ⅴ组外其余各组显著降低(P<0.05),1～42 d除了处理Ⅱ组外其余各组显著降低(P<0.05);粗蛋白质表观消化率方面,处理Ⅰ、Ⅲ、Ⅶ组比对照组显著增加(P<0.05),相比抗生素组,除处理Ⅳ组外其余各试验组显著增加(P<0.05);粗脂肪表观消化率方面,处理Ⅰ、Ⅱ、Ⅲ、Ⅶ组比对照组显著增加(P<0.05),与抗生素组比,除添加CAT组外其余显著增加(P<0.05);与对照组相比,各处理组钙和磷表观消化率差异不显著(P>0.05);能量表观消化率上,与对照组和抗生素组相比,除处理Ⅳ、Ⅴ组外其余各试验组显著增加(P<0.05)。因此,从结果来看,过氧化氢酶和葡萄糖氧化酶能够促进肉鸡的生长,增加肉鸡的养分表观消化率;在适宜添加量的前提下,能够替代抗生素。
In the test, the purpose is to study the influence of glucose oxidase and catalase on broil-er's growth performance and nutrient digestibility, which fed by diet based on corn-soybean meal.1 080 healthy and one-day broilers(male 50%, female 50%) were randomly divided into nine groups ac-cording to the test requirements. The control group, antibiotic group, treatment group Ⅰ, Ⅱ and Ⅲ(added glucose oxidase of 150 g/t, enzyme activity is 1 000, 2 000 U/g and 4 000 U/g respectively), treat-ment group Ⅳ, Ⅴ and Ⅵ(added catalase of 150 g/t, enzyme activity is 5 000, 10 000, 20 000 U/g re-spectively), treatment group Ⅶ(added compound enzyme 150 g/t glucose oxidase activity is 2 000 U/g,catalase activity is 10 000 U/g). The results showed that, compared with the control group and theantibiotic group, feed conversion rate of the 1～21 day was decreased significantly(P<0.05). The 22～42 days was decreased significantly(P<0.05), except for the group Ⅱ and Ⅴ. The 1～42 dayswas decreased significantly(P<0.05), except forthe group Ⅱ. Compared with control group, theapparent nutrition digestion efficiency of CP of group Ⅰ, Ⅲ and Ⅶ increased significantly(P<0.05), compared with the antibiotic group, increased sig-nificantly(P<0.05), except for the group Ⅵ. Compared with control group, the apparent nutrition diges-tion efficiency of CF of group Ⅰ, Ⅱ, Ⅲ and Ⅶ increased significantly(P<0.05), compared with the anti-biotic group, increased significantly(P<0.05), except for the group CAT. Compared with control group, di-gestion efficiency of phosphorus and calcium were not significant(P>0.05). Compared with control groupand the antibiotic group, every group of the energy apparent digestibility was increased significantly(P<0.05), except for group Ⅳ and Ⅴ. Therefore, from the results, catalase and glucose oxidase can promotethe growth of chicken and increase the apparent nutrition digestibility of broiler chickens; Under thepremise of suitable dosage, it can replace antibiotics.
In the test, the purpose is to study the influence of glucose oxidase and catalase on broil-er's growth performance and nutrient digestibility, which fed by diet based on corn-soybean meal.1 080 healthy and one-day broilers(male 50%, female 50%) were randomly divided into nine groups ac-cording to the test requirements. The control group, antibiotic group, treatment group Ⅰ, Ⅱ and Ⅲ(added glucose oxidase of 150 g/t, enzyme activity is 1 000, 2 000 U/g and 4 000 U/g respectively), treat-ment group Ⅳ, Ⅴ and Ⅵ(added catalase of 150 g/t, enzyme activity is 5 000, 10 000, 20 000 U/g re-spectively), treatment group Ⅶ(added compound enzyme 150 g/t glucose oxidase activity is 2 000 U/g,catalase activity is 10 000 U/g). The results showed that, compared with the control group and theantibiotic group, feed conversion rate of the 1～21 day was decreased significantly(P<0.05). The 22～42 days was decreased significantly(P<0.05), except for the group Ⅱ and Ⅴ. The 1～42 dayswas decreased significantly(P<0.05), except forthe group Ⅱ. Compared with control group, theapparent nutrition digestion efficiency of CP of group Ⅰ, Ⅲ and Ⅶ increased significantly(P<0.05), compared with the antibiotic group, increased sig-nificantly(P<0.05), except for the group Ⅵ. Compared with control group, the apparent nutrition diges-tion efficiency of CF of group Ⅰ, Ⅱ, Ⅲ and Ⅶ increased significantly(P<0.05), compared with the anti-biotic group, increased significantly(P<0.05), except for the group CAT. Compared with control group, di-gestion efficiency of phosphorus and calcium were not significant(P>0.05). Compared with control groupand the antibiotic group, every group of the energy apparent digestibility was increased significantly(P<0.05), except for group Ⅳ and Ⅴ. Therefore, from the results, catalase and glucose oxidase can promotethe growth of chicken and increase the apparent nutrition digestibility of broiler chickens; Under thepremise of suitable dosage, it can replace antibiotics.
引文
[1]Caroline A,Yannig N,Olivier O,et al.Glucose oxidase bioanodes for glucose conversion and H2O2 production for horseradish peroxidase biocathodes in a flow through glucose biofuel cell design[J].Journal of Power Sources,2018,392:176-180.
[2]Scibior D,Czeczot H.Katalaza-budowa,w?a?ciwo?ci,funkcje[Catalase:structure,properties,functions][J].Postepy Hig Med Dosw(Online),2006,60:170-180.
[3]Gracia M I,Lázaro R,Latorre M A,et al.Influence of enzyme supplementation of diets and cooking-flaking of maize on digestive traits and growth performance of broilers from 1 to 21 days of age[J].Animal Feed Science and Technology,2009,150(3/4):303-315.
[4]Loew O.A new enzyme of general occurrence in organisms[J].Science,1900,11(279):701-702.
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[6]Yan Li.The Experiment of Glucose Oxidase Used in Broiler[J].Journal of Longyan Teachers College,2004,22(6):77-78.
[7]方锐,左建军,凌宝明,等.日粮中添加过氧化氢酶对断奶仔猪生长性能、肠道形态及抗氧化性能的影响[J].中国饲料,2017(1):23-27.
[8]庞家满,王江,李杰,等.葡萄糖氧化酶对黄羽肉鸡生产性能和养分代谢的影响[J].中国畜牧兽医,2013,40(2):72-75.
[9]刘永超,刘宁,石学刚.低能日粮添加木聚糖酶或复合酶对肉鸡生产性能和养分消化率的影响[J].饲料工业,2011,32(16):39-42.
[10]宋海彬,赵国先,李娜,等.葡萄糖氧化酶及其在畜牧生产中的应用[J].饲料与畜牧,2008(7):37-39.
[11]宋海彬,赵国先,刘彦慈,等.葡萄糖氧化酶对肉鸡肠道形态结构和消化酶活性的影响[J].中国畜牧杂志,2010,46(23):56-59.
[12]杨佳栋,魏凤菊,潘新新,等.动物过氧化氢酶(CAT)的研究进展[J].黑龙江畜牧兽医,2016(13):59-62.
[13]赵国先,张小云,左晓磊,等.葡萄糖氧化酶对蛋鸡生产性能的影响[J].河北农业大学学报,2007(1):89-92.
[14]赵国先,张晓云,计成,等.葡萄糖氧化酶对蛋鸡日粮主要营养物质表观消化率及肠道微生物的调控作用(英文)[J].动物营养学报,2008,20(6):679-685.
[2]Scibior D,Czeczot H.Katalaza-budowa,w?a?ciwo?ci,funkcje[Catalase:structure,properties,functions][J].Postepy Hig Med Dosw(Online),2006,60:170-180.
[3]Gracia M I,Lázaro R,Latorre M A,et al.Influence of enzyme supplementation of diets and cooking-flaking of maize on digestive traits and growth performance of broilers from 1 to 21 days of age[J].Animal Feed Science and Technology,2009,150(3/4):303-315.
[4]Loew O.A new enzyme of general occurrence in organisms[J].Science,1900,11(279):701-702.
[5]Wilson R,Turner A P F.Glucose oxidase:an ideal enzyme[J].Biosensors and Bioelectronics,1992,7(3):165-185.
[6]Yan Li.The Experiment of Glucose Oxidase Used in Broiler[J].Journal of Longyan Teachers College,2004,22(6):77-78.
[7]方锐,左建军,凌宝明,等.日粮中添加过氧化氢酶对断奶仔猪生长性能、肠道形态及抗氧化性能的影响[J].中国饲料,2017(1):23-27.
[8]庞家满,王江,李杰,等.葡萄糖氧化酶对黄羽肉鸡生产性能和养分代谢的影响[J].中国畜牧兽医,2013,40(2):72-75.
[9]刘永超,刘宁,石学刚.低能日粮添加木聚糖酶或复合酶对肉鸡生产性能和养分消化率的影响[J].饲料工业,2011,32(16):39-42.
[10]宋海彬,赵国先,李娜,等.葡萄糖氧化酶及其在畜牧生产中的应用[J].饲料与畜牧,2008(7):37-39.
[11]宋海彬,赵国先,刘彦慈,等.葡萄糖氧化酶对肉鸡肠道形态结构和消化酶活性的影响[J].中国畜牧杂志,2010,46(23):56-59.
[12]杨佳栋,魏凤菊,潘新新,等.动物过氧化氢酶(CAT)的研究进展[J].黑龙江畜牧兽医,2016(13):59-62.
[13]赵国先,张小云,左晓磊,等.葡萄糖氧化酶对蛋鸡生产性能的影响[J].河北农业大学学报,2007(1):89-92.
[14]赵国先,张晓云,计成,等.葡萄糖氧化酶对蛋鸡日粮主要营养物质表观消化率及肠道微生物的调控作用(英文)[J].动物营养学报,2008,20(6):679-685.
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