盐胁迫下商洛丹参对外源钙的生理响应
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摘要
研究了不同浓度(10、20、30、40、50 mmol/L) CaCl2对盐胁迫(100 mmol/L NaCl)下一年生丹参幼苗相关生理指标的影响。结果表明:与CK1(蒸馏水对照)相比,CK2(100 mmol/L NaCl对照)下丹参幼苗的各项生理指标均受到了显著影响;随着施用外源钙浓度的增加,丹参幼苗中CAT、POD活性,以及可溶性糖、可溶性蛋白、叶绿素及Pro含量均呈先升高后降低的变化趋势,而丙二醛含量呈先降低后升高的变化趋势;与CK2相比,在处理T3(30 mmol/L CaCl2+100 mmol/L NaCl)下,MDA含量下降了36.4%,CAT、POD活性以及可溶性糖、可溶性蛋白、Pro和叶绿素含量分别提高了65%、130%、98%、82%、75%和164%,差异显著(P<0.05)。可见,施用一定浓度的CaCl2能有效缓解盐胁迫对丹参幼苗生长发育的抑制作用,增强丹参的耐盐性。
The effects of different concentrations(10,20,30,40 and 50 mmol/L) of CaCl2 on several physiological indexes of one-year-old Salvia miltiorrhiza seedlings under salt(100 mmol/L NaCl) stress were studied. The results showed that: in comparison with CK1(distilled water control),the various physiological indexes of S. miltiorrhiza seedlings were significantly affected by CK2(100 mmol/L NaCl control); when the concentration of applied exogenous calcium increased,the activities of CAT and POD,as well as the contents of soluble sugar,soluble protein,chlorophyll and Pro in S. miltiorrhiza seedlings all increased first and then decreased,while the content of MDA decreased first and then increased. In comparison with CK2,the treatment T3(30 mmol/L CaCl2+100 mmol/L NaCl) significantly reduced the MDA content by 36.4%,significantly increased the activities of CAT and POD by 65% and 130% respectively,and significantly enhanced the contents of soluble sugar,soluble protein,Pro and chlorophyll by 98%,82%,75% and 164% separately. Thus,applying a certain concentration of CaCl2 could effectively alleviate the inhibitory effects of salt stress on the growth and development of S. miltiorrhiza seedlings,and enhance their tolerance to salt.
The effects of different concentrations(10,20,30,40 and 50 mmol/L) of CaCl2 on several physiological indexes of one-year-old Salvia miltiorrhiza seedlings under salt(100 mmol/L NaCl) stress were studied. The results showed that: in comparison with CK1(distilled water control),the various physiological indexes of S. miltiorrhiza seedlings were significantly affected by CK2(100 mmol/L NaCl control); when the concentration of applied exogenous calcium increased,the activities of CAT and POD,as well as the contents of soluble sugar,soluble protein,chlorophyll and Pro in S. miltiorrhiza seedlings all increased first and then decreased,while the content of MDA decreased first and then increased. In comparison with CK2,the treatment T3(30 mmol/L CaCl2+100 mmol/L NaCl) significantly reduced the MDA content by 36.4%,significantly increased the activities of CAT and POD by 65% and 130% respectively,and significantly enhanced the contents of soluble sugar,soluble protein,Pro and chlorophyll by 98%,82%,75% and 164% separately. Thus,applying a certain concentration of CaCl2 could effectively alleviate the inhibitory effects of salt stress on the growth and development of S. miltiorrhiza seedlings,and enhance their tolerance to salt.
引文
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[20]朱利君,闫秋洁.外源氯化钙对大蒜幼苗盐胁迫伤害的缓解作用[J].江苏农业科学,2016,44(8):242-244.
[21]李百伟,孙存华. CaCl2对NaCl胁迫下甘薯幼苗叶片SOD和POD活性的影响[J].新技术应用与实践,2018(3):183-184.
[22]石全梅.外源GA3对盐胁迫下药用丹参生理特性的影响[D].南京:南京农业大学,2015:39-73.
[23]华智锐,李小玲.外源硅对盐胁迫下黄芩幼苗生理生化特性的影响[J].江西农业学报,2018,30(3):23-27.
[24]刘新星,罗俊杰.外源钙对盐胁迫下豌豆幼苗生化指标的影响[J].江西农业学报,2013,25(2):24-27.
[25]王淑智,李利,张道勇,等.NaCl与Cd对小球藻光系统Ⅱ(PSⅡ)活性的影响[J].应用与环境生物学报,2011,17(6):839-846.
[2]Najafi F,Khavari-Nejad R A,Siah A M. The effects ofsalt stress on certain physiological parameters in summersavory(Satureja hortensis L.)plants[J]. Journal ofStress Physiology&Biochemistry,2010,6(1):13-21.
[3]牛灵慧,朱光明,王康才,等.盐胁迫对丹参种子萌发及幼苗生长的影响[J].安徽农业大学学报,2016,43(4):651-657.
[4]潘瑞炽,王小菁,李娘辉,等.植物生理学[M].北京:高等教育出版社,2009.
[5]霍书新,杜国强,张小红,等.钙缓解植物盐害的作用机制研究进展[J].农药市场信息,2005(6):3-6.
[6]王涵,杨娜,谭静,等.丹参化学成分、药理作用及临床应用的研究进展[J].特产研究,2018(1):48-53.
[7]刘艾林,李铭源,王一涛,等.丹参药理学活性物质基础研究现状[J].中国药学杂志,2007,42(9):641-646.
[8]刘丽,曾长立.外源钙对盐胁迫下菠菜生理特性的影响[J].湖北农业科学,2015,54(1):118-122.
[9]Chen H X,Li W J,An S Z. The role of calcium in allevi-ating photoinhibition in NaCl stressed leaves of Rumex K-lseedlings[J]. Journal of Plant Physiology and MolecularBiology,2003,29(5):440-445.
[10]周双云,蒋晶,高龙燕,等.不同浓度CaCl2对盐胁迫下巴西蕉幼苗生理的影响[J].应用与环境生物学报,2014,20(3):449-454.
[11]郑秀芳,张超强.外源钙对盐胁迫下马铃薯试管苗生长和相关生理特性的影响[J].西北农业学报,2015,24(6):97-102.
[12]Gene Y,Tester M,McDonald G K. Calcium requirementof wheat in saline and nonsaline conditions[J]. PlantSoil,2010,327:331-345.
[13]魏永鹏,陈天祥,南丽丽,等.盐胁迫下外源Ca2+对红豆草幼苗抗性生理的影响[J].草原与草坪,2015,35(1):15-20.
[14]杨莎,侯林琳,郭峰,等.盐胁迫下外源Ca2+对花生生长发育、生理及产量的影响[J].应用生态学报,2017,28(3):894-900.
[15]张志良,瞿伟菁,李小方,等.植物生理学实验指导[M].北京:高等教育出版社,2009.
[16]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[17]赵世杰,史国安,董新纯,等.植物生理学实验指导[M].北京:中国农业科学技术出版社,2002.
[18]朱广联,钟诲文,张爱琴.植物生理学实验[M].北京:北京大学出版社,1990.
[19]杨立飞,朱月林,胡春梅,等.NaCl胁迫对嫁接黄瓜膜脂过氧化、渗透调节物质含量及光合特性的影响[J].西北植物学报,2006,26(6):1195-1200.
[20]朱利君,闫秋洁.外源氯化钙对大蒜幼苗盐胁迫伤害的缓解作用[J].江苏农业科学,2016,44(8):242-244.
[21]李百伟,孙存华. CaCl2对NaCl胁迫下甘薯幼苗叶片SOD和POD活性的影响[J].新技术应用与实践,2018(3):183-184.
[22]石全梅.外源GA3对盐胁迫下药用丹参生理特性的影响[D].南京:南京农业大学,2015:39-73.
[23]华智锐,李小玲.外源硅对盐胁迫下黄芩幼苗生理生化特性的影响[J].江西农业学报,2018,30(3):23-27.
[24]刘新星,罗俊杰.外源钙对盐胁迫下豌豆幼苗生化指标的影响[J].江西农业学报,2013,25(2):24-27.
[25]王淑智,李利,张道勇,等.NaCl与Cd对小球藻光系统Ⅱ(PSⅡ)活性的影响[J].应用与环境生物学报,2011,17(6):839-846.
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