不同培育措施对降香黄檀保护性酶、PAL活性及MDA含量的影响
|
摘要
为了明确移植、钾肥、修枝、乙烯四种培育措施对降香黄檀保护性酶、PAL活性及MDA含量的影响,以胸径6～8 cm幼龄降香黄檀为研究对象,研究了不同钾肥用量、修枝强度、乙烯百分数及移植中机械处理方法引起的降香黄檀SOD、POD、CAT、PAL活性与MDA含量的变化规律。结果表明:移植、钾肥、修枝、乙烯四种培育措施均对降香黄檀叶片保护性酶、PAL活性及MDA有不同程度的影响;四种酶活性在移植各处理中以全冠移>断根>去冠>去冠移>CK为顺序依次升高;与修枝强度、钾肥用量呈明显的正相关关系,且随乙烯百分比增加先增大后减小。MDA含量增加与修枝强度和钾肥的用量呈负相关关系,随乙烯百分数增加先降后升,移植试验中以CK>去冠移>去冠>断根>全冠移各处理为顺序依次增加。
Aiming to determine the effects of four cultivation methods including transplantation, potash fertilizer,pruning, and ethylene on the protective enzymes, PAL activity and MDA content of Dalbergia odorifera, young Dalbergia odorifera 6～8 cm in diameter was selected as the study object. The change regulation of SOD, POD,CAT, PAL activity and MDA content in Dalbergia odorifera were studied with different potash dosage, pruning strength, percentage of ethylene and mechanical treatment during transplantation. The results showed that the four cultivation methods which were transplantation, potash fertilizer, pruning, and ethylene, all had varying degrees of effects on protective enzymes, PAL activity and MDA content in leaves of Dalbergia odorifera. The activities of four enzymes increased in the order of full crown shift>root break>crown removal>crown shift>CK. There was a significant positive correlation between pruning intensity and potassium fertilizer dosage, and they increased first and then decreased with increasing ethylene percentage. The increase in MDA content was negatively correlated with the pruning intensity and the amount of potash fertilizer, with the ethylene percentage increased, it dropped first and then rises; in the transplantation experiment, the order of CK >de-crown shift >crown removal >root removal>full crown shift was sequentially increased.
Aiming to determine the effects of four cultivation methods including transplantation, potash fertilizer,pruning, and ethylene on the protective enzymes, PAL activity and MDA content of Dalbergia odorifera, young Dalbergia odorifera 6～8 cm in diameter was selected as the study object. The change regulation of SOD, POD,CAT, PAL activity and MDA content in Dalbergia odorifera were studied with different potash dosage, pruning strength, percentage of ethylene and mechanical treatment during transplantation. The results showed that the four cultivation methods which were transplantation, potash fertilizer, pruning, and ethylene, all had varying degrees of effects on protective enzymes, PAL activity and MDA content in leaves of Dalbergia odorifera. The activities of four enzymes increased in the order of full crown shift>root break>crown removal>crown shift>CK. There was a significant positive correlation between pruning intensity and potassium fertilizer dosage, and they increased first and then decreased with increasing ethylene percentage. The increase in MDA content was negatively correlated with the pruning intensity and the amount of potash fertilizer, with the ethylene percentage increased, it dropped first and then rises; in the transplantation experiment, the order of CK >de-crown shift >crown removal >root removal>full crown shift was sequentially increased.
引文
Bowler C.,Van Montagu T.,and Inze D.,1992,Superoxide dismutase and stress tolerance,Ann.Rev.Plant Physiol.Plant Mol.Biol.,43(1):83-116
Dhindsa R.S.,Dhindsa P.P.,and Thorpe T.A.,1981,Leaf senescence,correlated with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase,J.Exp.Bot.,32(1):93-101
Fan J.S.,Chen Y.G.,Li K.R.,and Ma C.Q.,2006,Effect of soil moisture condition on the growth and yield of Juglans regia,Linye Kexue(Scientia Silvae Sinicae),42(12):39-46(樊金拴,陈原国,李凯荣,马创奇,2006,土壤水分状况对核桃生长和发育的影响,林业科学,42(12):39-46)
Fridovich I.,1986,Biological effects of the superoxide radical,Arch.Biochem.Biophys.,247(1):1-11
Ghosh A.,Chikara J.,and Chaudhary D.R.,2011,Diminution of economic yield as affected by pruning and chemical manipula tion of Jatropha curcas L.,Biomass Bioenerg,35(3):1021-1029
Ghosh A.,Chikara J.,Chaudhary D.R.,Prakash A.R.,Boricha G.,and Zala A.,2010,Paclobutrazol arrests vegetative growth and unveils unexpressed yield potential of Jatropha curcas,J.Plant Growth Regul.,29(3):307-315
Giannopolitis C.N.,and Ries S.K.,1977,Superoxide dismutase:I.occurrence in higher plants,Plant Physiol.,59(2):309-314
Huang M.,2016,Alleviation effects of ethylene and H2O2on waterlogging stress and the underling mechanisms in wheat,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Jiang D.,pp.56(黄梅,2016,乙烯和过氧化氢对小麦渍害的缓减效应及其生理机制研究,硕士学位论文,南京农业大学,导师:姜东,pp.56)
Hu D.M.,Sun M.G.,Wang T.M.,Li X.F.,and Li C.,2005,Effect of sulfur dioxide stress on membrane lipid peroxidation and protective enzymes of three ornamental tree seedlings,Shandong Nongye Daxue Xuebao(Journal of Shandong Agricultural University)(Natural Science),36(2):175-180(胡丁猛,孙明高,王太明,李秀芬,李成,2005,SO2对三种园林绿化苗木叶片膜脂过氧化和保护酶的影响,山东农业大学学报(自然科学版),36(2):175-180)
Jiang X.D.,Jiang L.L.,Hua M.F.,Chen H.L.,Lv R.,Hu N.,and Yang X.Y.,2018,Analysis the effect of different chemical agents on high temperature stress in rice leaves,Zhongguo Nongye Qixiang(Chinese Journal of Agrometeorology),39(2):92-99(江晓东,姜琳琳,华梦飞,陈惠玲,吕润,胡凝,杨晓亚,2018,喷施不同化学制剂对水稻叶片抗高温胁迫的效果分析,中国农业气象,39(2):92-99)
Jia R.F.,2014,Studies on artifcially induced heartwood formation of Dalbergia odorifera,Dissertation for Ph.D.,Chinese Academy of Forestry Sciences,Supervisor:Xu D.P.,pp.14-17(贾瑞丰,2014,降香黄檀人工促进心材形成的研究,博士学位论文,中国林业科学研究院,导师:徐大平,pp.14-17)
Koukol J.,and Conne E.E.,1961,The metabolism of aromatic:compounds in higher plants.V.Purification and properties of the phenylalanine deaminase of Herdeum vulagare,J.Biol.Chem.,236(10):2692-2698
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.X.,Li Y.J.,and Wang B.Z.,2014,Study on rapid propagation technology of Dalbergia odorifera T.Chen.,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(4):902-905(林妃,李敬阳,黄东梅,许奕,李艳霞,李羽佳,王必尊,2014,花梨木快繁技术的研究,基因组学与应用生物学,33(4):902-905)
Li Z.T.,Chen E.Y.,Zhang M.L.,Zhao Q.L.,Xu X.L.,Ji H.,Song X.L.,and Sun X.Z.,2012,Effect of potassium application methods on antioxidant enzyme activities,yield,and potas sium use efficiency of cotton,Zuowu Xuebao(Acta Agronomica Sinica),38(3):487-494(李宗泰,陈二影,张美玲,赵庆龙,许晓龙,姬红,宋宪亮,孙学振,2012,施钾方式对棉花叶片抗氧化酶活性、产量及钾肥利用效率的影响,作物学报,38(3):487-494)
Papadimitriou E.,and Loumbourdis N.S.,2002,Exposure of the frog Rana ridibunda to copper impact on two biomarkers,lipid peroxidation,and glutathione,Bull.Environ.Contam.Toxicol.,69(6):885-891
Sun G.R.,Guan Y.,and Yan X.F.,2001,Effect of Na2CO3stress on defensive enzyme system of Puccinellia tenuiflora seedlings,Acta Agrestia Sinica,9(1):34-38
Suzuki N.,and Mittler R.,2006,Reactive oxygen species and temperature stresses:a delicate balance between signaling and destruction,Physiol.Plant.,126(1):45-51
Wang L.Y.,Liu X.J.,Xu D.P.,Yang Z.J.,Zhang N.N.,Hong Z.,Zhong W.B.,and Yuan Y.Q.,2017,Effects of plant growth regulators on vegetative and reproductive growth of Dalbergia odorifera,Huanan Nongye Daxue Xuebao(Journal of South China Agricultural University),38(5):86-90(王丽云,刘小金,徐大平,杨曾奖,张宁南,洪舟,钟文斌,袁雅琪,2017,生长调节剂对降香黄檀营养生长与生殖生长的影响,华南农业大学学报,38(5):86-90)
Wang Y.F.,Han J.F.,and Lin X.W.,1996,Study on physiological and biochemical responses of flue-cured tobacco to drought stress during early growth of the plants,Zuowu Xuebao(Acta Agronomica Sinica),22(1):117-122(汪耀富,韩锦峰,林学梧,1996,烤烟生长前期对干旱胁迫的生理生化响应研究,作物学报,22(1):117-122)
Wang Y.P.,Yang J.N.,Wang D.D.,Yang Q.,Zhang X.M.,and Shen Y.Y.,2017,Effects of clipping height on stress resistance and antioxidant enzymes activities of dual-purpose winter wheat,Caodi Xuebao(Acta Agrestia Sinica),25(4):762-767(王玉平,杨景宁,王丹丹,杨倩,张小明,沈禹颖,2017,刈割高度对粮饲兼用小麦抗逆性及保护性酶活性的影响,草地学报,25(4):762-767)
Wu G.X.,Wang L.H.,Liang H.P.,Li Y.F.,and Hao J.,2012,Fertilizer treatments for growth and physiology of Dalbergia odorifera seedlings,Zhejiang Nonglin Daxue Xuebao(Journal of Zhejiang A&F University),29(2):296-300(吴国欣,王凌晖,梁惠萍,李远发,郝建,2012,氮磷钾配比施肥对降香黄檀苗木生长及生理的影响,浙江农林大学学报,29(2):296-300)
Wu Z.H.,Zeng F.H.,Ma S.J.,Xie Y.J.,and Lu X.Y.,2004,A review of advances in active oxygen metabolism in plants under water stress,Yaredai Zhiwu Kexue(Subtropical Plant Science),33(3):77-80(吴志华,曾富华,马生健,谢耀坚,卢向阳,2004,水分胁迫下植物活性氧代谢研究进展(综述Ⅰ),亚热带植物科学,33(3):77-80)
Yeshitela T.,Robbertse P.J.,and Stassen P.J.C.,2004,Paclobutrazol suppressed vegetative growth and improved yield as well as fruit quality of'Tommy Atkins'mango(Mangifera indica)in Ethiopia,New Zeal.J.Crop.Hort.Sci.,32(3):281-293
Ye X.F.,Zhu H.B.,Jin D.M.,Liu G.S.,Wang Y.Y.,Cui S.Y.,Yu Q.W.,and Zhang S.,2007,Influence of different potassium fertilizer on some enzyme activities of growing flue-cured tobacco,Huabei Nongxuebao(Acta Agriculturae BorealiSinica),22(2):67-70(叶协锋,朱海滨,靳冬梅,刘国顺,王英元,崔树毅,喻奇伟,张胜,2007,不同种类钾肥对烤烟生长过程中几种酶活性的影响,华北农学报,22(2):67-70)
Zhang M.Q.,Chen R.K.,and Yu S.L.,1996,The mathematical analysis on metabolism of active oxygen of sugarcane under water stress,Acta Agronomica Sinica,22(6):263-267
Zhang Y.Y.,Zhu Y.J.,Mu Z.,Wang Z.M.,Ou C.M.,and Rong Y.P.,2015,Physiological characteristics of Apocynum venetum under the stress of drought and heat,Caodi Xuebao(Acta Agrestia Sinica),23(5):1119-1122(张艳艳,朱玉浩,穆蓁,王忠美,欧成明,戎郁萍,2015,干热胁迫对罗布麻幼苗生理特性的影响,草地学报,23(5):1119-1122)
Dhindsa R.S.,Dhindsa P.P.,and Thorpe T.A.,1981,Leaf senescence,correlated with increased levels of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase,J.Exp.Bot.,32(1):93-101
Fan J.S.,Chen Y.G.,Li K.R.,and Ma C.Q.,2006,Effect of soil moisture condition on the growth and yield of Juglans regia,Linye Kexue(Scientia Silvae Sinicae),42(12):39-46(樊金拴,陈原国,李凯荣,马创奇,2006,土壤水分状况对核桃生长和发育的影响,林业科学,42(12):39-46)
Fridovich I.,1986,Biological effects of the superoxide radical,Arch.Biochem.Biophys.,247(1):1-11
Ghosh A.,Chikara J.,and Chaudhary D.R.,2011,Diminution of economic yield as affected by pruning and chemical manipula tion of Jatropha curcas L.,Biomass Bioenerg,35(3):1021-1029
Ghosh A.,Chikara J.,Chaudhary D.R.,Prakash A.R.,Boricha G.,and Zala A.,2010,Paclobutrazol arrests vegetative growth and unveils unexpressed yield potential of Jatropha curcas,J.Plant Growth Regul.,29(3):307-315
Giannopolitis C.N.,and Ries S.K.,1977,Superoxide dismutase:I.occurrence in higher plants,Plant Physiol.,59(2):309-314
Huang M.,2016,Alleviation effects of ethylene and H2O2on waterlogging stress and the underling mechanisms in wheat,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Jiang D.,pp.56(黄梅,2016,乙烯和过氧化氢对小麦渍害的缓减效应及其生理机制研究,硕士学位论文,南京农业大学,导师:姜东,pp.56)
Hu D.M.,Sun M.G.,Wang T.M.,Li X.F.,and Li C.,2005,Effect of sulfur dioxide stress on membrane lipid peroxidation and protective enzymes of three ornamental tree seedlings,Shandong Nongye Daxue Xuebao(Journal of Shandong Agricultural University)(Natural Science),36(2):175-180(胡丁猛,孙明高,王太明,李秀芬,李成,2005,SO2对三种园林绿化苗木叶片膜脂过氧化和保护酶的影响,山东农业大学学报(自然科学版),36(2):175-180)
Jiang X.D.,Jiang L.L.,Hua M.F.,Chen H.L.,Lv R.,Hu N.,and Yang X.Y.,2018,Analysis the effect of different chemical agents on high temperature stress in rice leaves,Zhongguo Nongye Qixiang(Chinese Journal of Agrometeorology),39(2):92-99(江晓东,姜琳琳,华梦飞,陈惠玲,吕润,胡凝,杨晓亚,2018,喷施不同化学制剂对水稻叶片抗高温胁迫的效果分析,中国农业气象,39(2):92-99)
Jia R.F.,2014,Studies on artifcially induced heartwood formation of Dalbergia odorifera,Dissertation for Ph.D.,Chinese Academy of Forestry Sciences,Supervisor:Xu D.P.,pp.14-17(贾瑞丰,2014,降香黄檀人工促进心材形成的研究,博士学位论文,中国林业科学研究院,导师:徐大平,pp.14-17)
Koukol J.,and Conne E.E.,1961,The metabolism of aromatic:compounds in higher plants.V.Purification and properties of the phenylalanine deaminase of Herdeum vulagare,J.Biol.Chem.,236(10):2692-2698
Lin F.,Li J.Y.,Huang D.M.,Xu Y.,Li Y.X.,Li Y.J.,and Wang B.Z.,2014,Study on rapid propagation technology of Dalbergia odorifera T.Chen.,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(4):902-905(林妃,李敬阳,黄东梅,许奕,李艳霞,李羽佳,王必尊,2014,花梨木快繁技术的研究,基因组学与应用生物学,33(4):902-905)
Li Z.T.,Chen E.Y.,Zhang M.L.,Zhao Q.L.,Xu X.L.,Ji H.,Song X.L.,and Sun X.Z.,2012,Effect of potassium application methods on antioxidant enzyme activities,yield,and potas sium use efficiency of cotton,Zuowu Xuebao(Acta Agronomica Sinica),38(3):487-494(李宗泰,陈二影,张美玲,赵庆龙,许晓龙,姬红,宋宪亮,孙学振,2012,施钾方式对棉花叶片抗氧化酶活性、产量及钾肥利用效率的影响,作物学报,38(3):487-494)
Papadimitriou E.,and Loumbourdis N.S.,2002,Exposure of the frog Rana ridibunda to copper impact on two biomarkers,lipid peroxidation,and glutathione,Bull.Environ.Contam.Toxicol.,69(6):885-891
Sun G.R.,Guan Y.,and Yan X.F.,2001,Effect of Na2CO3stress on defensive enzyme system of Puccinellia tenuiflora seedlings,Acta Agrestia Sinica,9(1):34-38
Suzuki N.,and Mittler R.,2006,Reactive oxygen species and temperature stresses:a delicate balance between signaling and destruction,Physiol.Plant.,126(1):45-51
Wang L.Y.,Liu X.J.,Xu D.P.,Yang Z.J.,Zhang N.N.,Hong Z.,Zhong W.B.,and Yuan Y.Q.,2017,Effects of plant growth regulators on vegetative and reproductive growth of Dalbergia odorifera,Huanan Nongye Daxue Xuebao(Journal of South China Agricultural University),38(5):86-90(王丽云,刘小金,徐大平,杨曾奖,张宁南,洪舟,钟文斌,袁雅琪,2017,生长调节剂对降香黄檀营养生长与生殖生长的影响,华南农业大学学报,38(5):86-90)
Wang Y.F.,Han J.F.,and Lin X.W.,1996,Study on physiological and biochemical responses of flue-cured tobacco to drought stress during early growth of the plants,Zuowu Xuebao(Acta Agronomica Sinica),22(1):117-122(汪耀富,韩锦峰,林学梧,1996,烤烟生长前期对干旱胁迫的生理生化响应研究,作物学报,22(1):117-122)
Wang Y.P.,Yang J.N.,Wang D.D.,Yang Q.,Zhang X.M.,and Shen Y.Y.,2017,Effects of clipping height on stress resistance and antioxidant enzymes activities of dual-purpose winter wheat,Caodi Xuebao(Acta Agrestia Sinica),25(4):762-767(王玉平,杨景宁,王丹丹,杨倩,张小明,沈禹颖,2017,刈割高度对粮饲兼用小麦抗逆性及保护性酶活性的影响,草地学报,25(4):762-767)
Wu G.X.,Wang L.H.,Liang H.P.,Li Y.F.,and Hao J.,2012,Fertilizer treatments for growth and physiology of Dalbergia odorifera seedlings,Zhejiang Nonglin Daxue Xuebao(Journal of Zhejiang A&F University),29(2):296-300(吴国欣,王凌晖,梁惠萍,李远发,郝建,2012,氮磷钾配比施肥对降香黄檀苗木生长及生理的影响,浙江农林大学学报,29(2):296-300)
Wu Z.H.,Zeng F.H.,Ma S.J.,Xie Y.J.,and Lu X.Y.,2004,A review of advances in active oxygen metabolism in plants under water stress,Yaredai Zhiwu Kexue(Subtropical Plant Science),33(3):77-80(吴志华,曾富华,马生健,谢耀坚,卢向阳,2004,水分胁迫下植物活性氧代谢研究进展(综述Ⅰ),亚热带植物科学,33(3):77-80)
Yeshitela T.,Robbertse P.J.,and Stassen P.J.C.,2004,Paclobutrazol suppressed vegetative growth and improved yield as well as fruit quality of'Tommy Atkins'mango(Mangifera indica)in Ethiopia,New Zeal.J.Crop.Hort.Sci.,32(3):281-293
Ye X.F.,Zhu H.B.,Jin D.M.,Liu G.S.,Wang Y.Y.,Cui S.Y.,Yu Q.W.,and Zhang S.,2007,Influence of different potassium fertilizer on some enzyme activities of growing flue-cured tobacco,Huabei Nongxuebao(Acta Agriculturae BorealiSinica),22(2):67-70(叶协锋,朱海滨,靳冬梅,刘国顺,王英元,崔树毅,喻奇伟,张胜,2007,不同种类钾肥对烤烟生长过程中几种酶活性的影响,华北农学报,22(2):67-70)
Zhang M.Q.,Chen R.K.,and Yu S.L.,1996,The mathematical analysis on metabolism of active oxygen of sugarcane under water stress,Acta Agronomica Sinica,22(6):263-267
Zhang Y.Y.,Zhu Y.J.,Mu Z.,Wang Z.M.,Ou C.M.,and Rong Y.P.,2015,Physiological characteristics of Apocynum venetum under the stress of drought and heat,Caodi Xuebao(Acta Agrestia Sinica),23(5):1119-1122(张艳艳,朱玉浩,穆蓁,王忠美,欧成明,戎郁萍,2015,干热胁迫对罗布麻幼苗生理特性的影响,草地学报,23(5):1119-1122)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |