银/稀土元素/碳微球抗菌剂的制备与表征
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摘要
以水热法合成的碳微球(carbonmicrospheres,CMSs)为载体,通过在硝酸银溶液和稀土硝酸盐溶液中浸渍使银和稀土元素吸附在碳微球上,得到载银碳微球抗菌剂(Ag/CMSs)和载银/稀土元素碳微球复合抗菌剂(Ag/Re/CMSs)。通过扫描电子显微镜(SEM)、X线衍射分析仪(XRD)、X线光电子能谱(XPS)、透射电子显微镜(TEM)和红外吸收光谱(FTIR)对产物的形貌和结构进行表征;采用抑菌环法,以大肠杆菌、白葡萄球菌和枯草芽孢杆菌为实验菌种对抗菌剂的抗菌性能进行定性分析。研究结果表明:使用水热法制备的碳微球表面光滑,粒径均一,单分散性良好;Ag/CMSs和Ag/Re/CMSs抗菌剂对大肠杆菌、白葡萄球菌和枯草芽孢杆菌均有很好的抑菌效果,其中负载银和铈的碳微球(Ag/Ce/CMSs)具有最强的抗菌效果。
Carbon microspheres(CMSs) as a carrier were synthesized by hydrothermal method. The Ag-loading carbon microspheres(Ag/CMSs) antibacterial agent and Ag/Re-loading carbon microspheres(Ag/Re/CMSs) composite antibacterial agent were prepared by immersing CMSs in a silver nitrate solution and rare earth nitrate solution. A series of investigations using scanning electron microscopy(SEM), X-ray diffraction(XRD), X-ray photoelectron spectrometry(XPS), transmission electron microscope(TEM) and Fourier transform infrared spectrometer(FTIR) were carried out to characterize the morphology and structure of samples. Qualitative analysis of antibacterial properties of antibacterial agent towards Escherichia col(E.coli), Staphylococcus aureus(S.aureus) and Bacillus subtilis(B.subtilis) were investigated by antibacterial ring method. The results show that the as-prepared carbon microspheres have smooth surface,uniform particle size and good monodisperse. Ag/CMSs and Ag/Re/CMSs antibacterial agents show good antibacterial effect for E.coli, S.aureus and B.subtilis.Among them,Ag/Ce/CMSs exhibits the strongest antibacterial effect.
Carbon microspheres(CMSs) as a carrier were synthesized by hydrothermal method. The Ag-loading carbon microspheres(Ag/CMSs) antibacterial agent and Ag/Re-loading carbon microspheres(Ag/Re/CMSs) composite antibacterial agent were prepared by immersing CMSs in a silver nitrate solution and rare earth nitrate solution. A series of investigations using scanning electron microscopy(SEM), X-ray diffraction(XRD), X-ray photoelectron spectrometry(XPS), transmission electron microscope(TEM) and Fourier transform infrared spectrometer(FTIR) were carried out to characterize the morphology and structure of samples. Qualitative analysis of antibacterial properties of antibacterial agent towards Escherichia col(E.coli), Staphylococcus aureus(S.aureus) and Bacillus subtilis(B.subtilis) were investigated by antibacterial ring method. The results show that the as-prepared carbon microspheres have smooth surface,uniform particle size and good monodisperse. Ag/CMSs and Ag/Re/CMSs antibacterial agents show good antibacterial effect for E.coli, S.aureus and B.subtilis.Among them,Ag/Ce/CMSs exhibits the strongest antibacterial effect.
引文
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[2]刘慧媛,辛正,王永明.稀土元素抑菌喷剂抗菌效果观察[J].中国消毒学杂志,2006,23(6):528-529.LIU Huiyuan,XIN Zheng,WANG Yongming.Observation on antimicrobial efficacy of rare earth element bacteriostatic spray[J].Chinese Journal of Disinfection,2006,23(6):528-529.
[3]陈爱美,施庆珊,欧阳友生,等.稀土抗菌效应及应用的研究进展[J].微生物学通报,2009,36(1):90-96.CHEN Aimei,SHI Qingshan,OUYANG Yousheng,et al.Progress in antimicrobial activity and application of rare earths[J].Microbiology,2009,36(1):90-96.
[4]杨永珍,刘伟峰,韩艳星,等.功能化碳微球构建自组装膜[J].化工学报,2011,62(4):1020-1026.YANG Yongzhen,LIU Weifeng,HAN Yanxing,et al.Self-assembly film of functionalized carbon microspheres[J].Journal of Chemical Industry and Engineering(China),2011,62(4):1020-1026.
[5]李莎,段菲菲,杨永珍,等.硅烷偶联剂对碳微球的表面化学修饰[J].功能材料,2011,42(1):25-29.LI Sha,DUAN Feifei,YANG Yongzhen,et al.Surface modification of carbon microspheres using a silane coupling agent[J].Journal of Functional Materials,2011,42(1):25-29.
[6]许并社,罗秋苹,杨永珍,等.银/碳微球复合材料的制备和表征[J].中国材料进展,2009,28(2):35-38.XU Bingshe,LUO Qiuping,YANG Yongzhen,et al.Preparation and characterization of Ag/carbon microspheres composites[J].Materials China,2009,28(2):35-38.
[7]ZHAO Ying,WANG Ziqiang,ZHAO Xin,et al.Antibacterial action of silver-doped activated carbon prepared by vacuum impregnation[J].Applied Surface Science,2013,266(2):67-72.
[8]梁金生,梁广川,祁洪飞,等.稀土复合磷酸盐无机抗菌材料对陶瓷活化水性能的影响[J].中国稀土学报,2003,21(2):241-243.LIANG Jinsheng,LIANG Guanchuan,QI Hongfei,et al.Influence of rare earth/composite phosphate inorganic antibacterial materials on activated water property of ceramic[J].Journal of the Chinese Rare Earth Society,2003,21(2):241-243.
[9]梁金生,金宗哲,王静.抗菌功能陶瓷材料的研究[J].河北工业大学学报,2000,29(5):45-47.LIANG Jinsheng,JIN Zongzhe,WANG Jing.Study on antibacterial ceramics[J].Journal of Hebei University of Technology,2000,29(5):45-47.
[10]李欢,唐晓宁,张彬,等.掺铒银型无机抗菌材料的制备与研究[J].硅酸盐通报,2017,36(2):629-634.LI Huan,TANG Xiaoning,ZHANG Bin,et al.Preparation and research on Ag-Er inorganic antibacterial materials doped erbium[J].Bulletin of the Chinese Ceramic Society,2017,36(2):629-634.
[11]蔡婷婷,张梦莹,刘镕玮,等.碳微球的水热法制备与表征[J].信息记录材料,2017,18(8):49-50.CAI Tingting,ZHANG Mengyin,LIU Rongwei,et al.Preparation and characterization of carbon microspheres by hydrothermal method[J].Information Recording Materials,2017,18(8):49-50.
[12]TIEN H W,HUANG Y L,YANG S Y,et al.The production of graphene nanosheets decorated with silver nanoparticles for use in transparent,conductive films[J].Carbon,2011,49(5):1550-1560.
[13]LIM E J,CHOI S M,MIN H S,et al.Highly dispersed Ag nanoparticles on nanosheets of reduced graphene oxide for oxygen reduction reaction in alkaline media[J].Electrochemistry Communications,2013,28(1):100-103.
[14]龚艳勃,戴晋明,侯文生,等.载银碳微球抗菌剂的制备及其抗菌性能的研究[J].化工新型材料,2016,44(4):97-99.GONG Yanbo,DAI Jinming,HOU Wensheng,et al.Research on the preparation and antibacterial activity of Ag/CMSs[J].New Chemical Materials,2016,44(4):97-99.
[15]段晓杰,徐丽明,王春仁.纳米银医用抗菌敷料抗菌性能检测方法的研究[J].药物分析杂志,2012,32(1):167-172.DUAN Xiaojie,XU Liming,WANG Chunren.Evaluation of antibacterial activity for medical silver nanoparticles-containing dressing[J].Chinese Journal of Pharmaceutical Analysis,2012,32(1):167-172.
[16]张利,刘春,魏丽乔,等.抗菌医用导尿管的抗感染性能测试[J].太原理工大学学报,2012,43(6):655-659.ZHANG Li,LIU Chun,WEI Liqiao,et al.The Property of anti-infectivity in antibacterial medical urethral catheter[J].Journal of Taiyuan University of Technology,2012,43(6):655-659.
[17]郝亚敏,杨维佳,闫翎鹏,等.三明治结构银碳复合材料的制备与表征[J].中国材料进展,2015,34(5):363-366.HAO Yamin,YANG Weijia,YAN Lingpeng,et al.Preparation and characterization of sandwich-like Ag-C-Ag nanocomposites[J].Materials China,2015,34(5):363-366.
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