林氏按蚊线粒体全基因组序列的测定及基于线粒体基因组的按蚊属系统发育分析（英文）
|
摘要
【目的】对林氏按蚊Anopheles lindesayi完整的线粒体基因组进行测序及分析,依据已知的线粒体基因组构建并讨论按蚊属蚊虫的分子系统发育关系。【方法】对林氏按蚊线粒体基因组进行测序、注释,并对其基本特征和基本组成进行分析。基于串联的13个蛋白质编码基因的核苷酸序列和氨基酸序列,用ML法和贝叶斯法构建林氏按蚊和按蚊属其他32种蚊虫的系统发育树,据此探讨按蚊属蚊虫的系统发育关系和系统分类。【结果】林氏按蚊线粒体基因组全长为15 366 bp,包含13个蛋白质编码基因,22个tR NA基因,2个rR NA基因和一段控制区。林氏按蚊线粒体基因组呈现明显的AT偏斜和GC偏斜,AT偏斜为正,GC偏斜为负。除了COX1使用TCG和ND5使用GTG作为起始密码子以外,其他蛋白质编码基因的起始密码子均遵循ATN原则;终止密码子为TAA或者T。除了tR NASer(AGN)以外,其他的tR NA基因均呈现典型的三叶草二级结构。控制区AT含量最高,为94. 54%。滑窗分析显示蛋白质编码基因是用于构建亚属或属水平系统发育关系的最佳分子标记。系统发育树强烈支持塞蚊亚属Cellia、按蚊亚属Anopheles、徕蚊亚属Nyssorhynchus和柯特蚊亚属Kerteszia均为单系群。小五斑按蚊An. atroparvus和四斑按蚊An. quadrimaculatus A这两个种聚到一起,从传统的形态分类上讲,它们和林氏按蚊均属于按蚊亚属按蚊系蚊虫。但本研究构建的4个系统发育树均显示,(小五斑按蚊An. atroparvus+四斑按蚊An. quadrimaculatus A)和林氏按蚊被属于迈蚊系的中华按蚊分开,这为两个系的分类提供了新的论点。【结论】本研究获得了林氏按蚊的完整的线粒体基因组,探析了按蚊属的线粒体基因组特征和系统发育关系,为进一步研究蚊科线粒体基因组和系统发育关系提供了依据。
【Aim】To sequence and analyze the complete mitochondrial genome(mtgenome) of Anopheles lindesayi,and to construct and discuss the molecular phylogenetic relationships of the genus Anopheles based on the known mtgenomes. 【Methods】The complete mtgenome of An. lindesayi was sequenced and annotated,and its general features and base composition were analyzed. The phylogenetic relationships between An. lindesayi and other 32 species in the genus Anopheles were reconstructed using maximum likelihood(ML) and Bayesian inference(BI) methods based on the concatenated nucleotide sequences and amino acid sequences of 13 protein-coding genes(PCGs),and the systematics of the genus Anopheles was discussed based on the phylogenetic analysis. 【Results】The complete mtgenome sequence of An. lindesayi is 15 366 bp in length,which contains 13 PCGs,22 tRNA genes,two rR NA genes,and one control region. The mtgenome has a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. The initiation codons of PCGs comply with the ATN rule except that COX1 uses TCG and ND5 uses GTG as the start codon,and the termination codon is TAA or incomplete T. The tRNAs have the typical clover-leaf structure,but tRNASer(AGN)has a large loop instead of the conserved stem-and-loop structure. The control region has the highest AT content(94. 54%). The sliding window analysis suggested that the PCGs are the most suitable markers to elucidate the phylogenetic relationships at the subgenus and genus levels. The phylogenetic analysis strongly supported each of the subgenera Cellia,Anopheles,Nyssorhynchus and Kerteszia to be a monophyletic group. An. lindesayi and An. atroparvus + An. quadrimaculatus A, the latter two traditionally classified as Anopheles Series, are separated by An. sinensis in Myzorhynchus Series on all the four phylogenetic trees,raising a new debate on the taxonomy of the two Series. 【Conclusion 】 This study obtained the complete mtgenome sequence of An.lindesayi and explored the mtgenome characteristics and phylogeny of the genus Anopheles,establishing the information frame for further studying the mtgenomes and phylogeny of the family Culicidae.
【Aim】To sequence and analyze the complete mitochondrial genome(mtgenome) of Anopheles lindesayi,and to construct and discuss the molecular phylogenetic relationships of the genus Anopheles based on the known mtgenomes. 【Methods】The complete mtgenome of An. lindesayi was sequenced and annotated,and its general features and base composition were analyzed. The phylogenetic relationships between An. lindesayi and other 32 species in the genus Anopheles were reconstructed using maximum likelihood(ML) and Bayesian inference(BI) methods based on the concatenated nucleotide sequences and amino acid sequences of 13 protein-coding genes(PCGs),and the systematics of the genus Anopheles was discussed based on the phylogenetic analysis. 【Results】The complete mtgenome sequence of An. lindesayi is 15 366 bp in length,which contains 13 PCGs,22 tRNA genes,two rR NA genes,and one control region. The mtgenome has a clear bias in nucleotide composition with a positive AT-skew and a negative GC-skew. The initiation codons of PCGs comply with the ATN rule except that COX1 uses TCG and ND5 uses GTG as the start codon,and the termination codon is TAA or incomplete T. The tRNAs have the typical clover-leaf structure,but tRNASer(AGN)has a large loop instead of the conserved stem-and-loop structure. The control region has the highest AT content(94. 54%). The sliding window analysis suggested that the PCGs are the most suitable markers to elucidate the phylogenetic relationships at the subgenus and genus levels. The phylogenetic analysis strongly supported each of the subgenera Cellia,Anopheles,Nyssorhynchus and Kerteszia to be a monophyletic group. An. lindesayi and An. atroparvus + An. quadrimaculatus A, the latter two traditionally classified as Anopheles Series, are separated by An. sinensis in Myzorhynchus Series on all the four phylogenetic trees,raising a new debate on the taxonomy of the two Series. 【Conclusion 】 This study obtained the complete mtgenome sequence of An.lindesayi and explored the mtgenome characteristics and phylogeny of the genus Anopheles,establishing the information frame for further studying the mtgenomes and phylogeny of the family Culicidae.
引文
Alzohairy AM,2011.BioEdit:an important software for molecular biology.Gerf.Bull.Biosci.,2(1):60-61.
Beard CB,Hamm DM,Collins FH,1993.The mitochondrial genome of the mosquito Anopheles gambiae:DNA sequence,genome organization,and comparisons with mitochondrial sequences of other insects.Insect Mol.Biol.,2(2):103-124.
Boore JL,1999.Animal mitochondrial genomes.Nucleic Acids Res.,27(8):1767-1780.
Boore JL,2006.The use of genome-level characters for phylogenetic reconstruction.Trends Ecol.Evol.,21(8):439-446.
Boore JL,Macey JR,Medina M,2005.Sequencing and comparing whole mitochondrial genomes of animals.Methods Enzymol.,395:311-348.
Cameron SL,2014.Insect mitochondrial genomics:implications for evolution and phylogeny.Annu.Rev.Entomol.,59:95-117.
Demari-Silva B,Foster PG,Oliveira TMPD,Bergo ES,Sanabani SS,Pessoa R,Sallum MAM,2015.Mitochondrial genomes and comparative analyses of Culex camposi,Culex coronator,Culex usquatus,and Culex usquatissimus,(Diptera:Culicidae),members of the coronator group.BMC Genomics,16:831.
Foley DH,Bryan JH,Yeates D,Saul A,1998.Evolution and systematics of Anopheles:insights from a molecular phylogeny of Australasian mosquitoes.Mol.Phylogenet.Evol.,9(2):262-275.
Glick JI,1992.Illustrated key to the female Anopheles of southwestern Asia and Egypt(Diptera:Culicidae).Mosq.Syst.,24(2):125-153.
Gutell RR,Lee JC,Cannone JJ,2002.The accuracy of ribosomal RNAcomparative structure models.Curr.Opin.Struct.Biol.,12(3):301-310.
Hao YJ,Zou YL,Ding YR,Xu WY,Yan ZT,Li XD,Fu WB,Li TJ,Chen B,2017.Complete mitochondrial genomes of Anopheles stephensi and An.dirus and comparative evolutionary mitochondriomics of 50 mosquitoes.Sci.Rep.,7(1):7666-7679.
Harbach RE,2007.The Culicidae(Diptera):a review of taxonomy,classification and phylogeny.Zootaxa,1668:591-638.
Harbach RE,Kitching IJ,2005.Reconsideration of anopheline mosquito phylogeny(Diptera:Culicidae:Anophelinae)based on morphological data.Syst.Biodivers.,3(4):345-374.
Harbach RE,Kitching IJ,2010.Phylogeny and classification of the Culicidae(Diptera).Syst.Entomol.,23(4):327-370.
Harbach RE,Kitching IJ,2016.The phylogeny of Anophelinae revisited:inferences about the origin and classification of Anopheles(Diptera:Culicidae).Zool.Scr.,45(1):34-47.
Harrison BA,Rattanarithikul R,Peyton EL,Mongkolpanya K,1990.Taxonomic changes,revised occurrence records and notes on the Culicidae of Thailand and neighboring countries.Mosq.Syst.,22(3):196-227.
Hua YQ,Ding YR,Yan ZT,Si FL,Luo QC,Chen B,2016.The complete mitochondrial genome of Anopheles minimus(Diptera:Culicidae)and the phylogenetics of known Anopheles mitogenomes.Insect Sci.,23(3):353-365.
Hua YQ,Yan ZT,Fu WB,He QY,Zhou Y,Chen B,2015.Sequencing and analysis of the complete mitogenome in Anopheles culicifacies species B(Diptera:Culicidae).Mitochondr.DNA Part A,27(4):2909-2910.
Kazutaka K,Standley DM,2013.MAFFT multiple sequence alignment software version 7:improvements in performance and usability.Mol.Biol.Evol.,30(4):772-780.
Kim H,Sames WS,Lee I,Lee D,Kim H,Rueda L,Klein T,2009.Overwintering of Anopheles lindesayi japonicus larvae in the Republic of Korea.J.Am.Mosq.Control Assoc.,25(1):32-37.
Kim MJ,Wang AR,Park JS,Kim I,2014.Complete mitochondrial genomes of five skippers(Lepidoptera:Hesperiidae)and phylogenetic reconstruction of Lepidoptera.Gene,549(1):97-112.
Krzywinski J,Grushko OG,Besansky NJ,2006.Analysis of the complete mitochondrial DNA from Anopheles funestus:an improved dipteran mitochondrial genome annotation and a temporal dimension of mosquito evolution.Mol.Phylogenet.Evol.,39(2):417-423.
Krzywinski J,Li C,Morris M,Conn JE,Lima JB,Povoa MM,Wilkerson RC,2011.Analysis of the evolutionary forces shaping mitochondrial genomes of a Neotropical malaria vector complex.Mol.Phylogenet.Evol.,58(3):469-477.
Krzywinski J,Wilkerson RC,Besansky NJ,2001.Evolution of mitochondrial and ribosomal gene sequences in Anophelinae(Diptera:Culicidae):implications for phylogeny reconstruction.Mol.Phylogenet.Evol.,18(3):479-487.
Librado P,Rozas J,2009.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Logue K,Chan ER,Phipps T,Small ST,Reimer L,Henryhalldin C,Sattabongkot J,Siba PM,Zimmerman PA,Serre D,2013.Mitochondrial genome sequences reveal deep divergences among Anopheles punctulatus sibling species in Papua New Guinea.Malar.J.,12(1):64-74.
Lowe TM,Eddy SR,1997.t RNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence.Nucleic Acids Res.,25(5):955-964.
Luo QC,Hao YJ,Meng F,Li TJ,Ding YR,Hua YQ,Chen B,2016.The mitochondrial genomes of Culex tritaeniorhynchus and Culex pipiens pallens(Diptera:Culicidae)and comparison analysis with two other Culex species.Parasit.Vectors,9(1):406-416.
Ma Y,Ma YJ,Lin L,Wang Y,2013.Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles(Diptera:Culicidae)in China:based on r DNA-ITS2 sequences.Chin.J.Vector Biol.Control,24(5):382-388.[马颖,马雅军,林琳,王琰,2013.基于r DNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究.中国媒介生物学及控制杂志,24(5):382-388]
Masta SE,Boore JL,2004.The complete mitochondrial genome sequence of the spider Habronattus oregonensis reveals rearranged and extremely truncated t RNAs.Mol.Biol.Evol.,21(5):893-902.
Masta SE,Boore JL,2008.Parallel evolution of truncated transfer RNAgenes in arachnid mitochondrial genomes.Mol.Biol.Evol.,25(5):949-959.
Mitchell SE,Cockburn AF,Seawright JA,1993.The mitochondrial genome of Anopheles quadrimaculatus species A:complete nucleotide sequence and gene organization.Genome,36(6):1058-1073.
Mohanty A,Swain S,Kar SK,Hazra RK,2009.Analysis of the phylogenetic relationship of Anopheles species,subgenus Cellia(Diptera:Culicidae)and using it to define the relationship of morphologically similar species.Infect.Genet.Evol.,9(6):1204-1224.
Moreno M,Marinotti O,Krzywinski J,Tadei WP,James AA,Achee N,Conn JE,2010.Complete mt DNA genomes of Anopheles darlingi,and an approach to anopheline divergence time.Malaria J.,9(1):127-127.
Negrisolo,E,Babbucci M,Patarnello T,2011.The mitochondrial genome of the ascalaphid owlfly Libelloides macaronius and comparative evolutionary mitochondriomics of neuropterid insects.BMC Genomics,12(1):221-246.
Ojala D,Montoya J,Attardi G,1981.TRNA punctuation model of RNAprocessing in human mitochondrial.Nature,290(5806):470-474.
Oliveira TMP,Foster PG,Bergo ES,Nagaki SS,Sanabani SS,Marinotti O,Marinotti PN,Sallum MAM,2016.Mitochondrial genomes of Anopheles(Kerteszia)(Diptera:Culicidae)from the Atlantic forest,Brazil.J.Med.Entomol.,53(4):tjw001.
Perna NT,Kocher TD,1995.Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes.J.Mol.Evol.,41(3):353-358.
Posada D,Crandall KA,1998.MODELTEST,Ver.3.06:testing the model of DNA substitution.Bioinformatics,14(9):817-818.
Reid JA,1968.Anopheline mosquitoes of Malaya and Borneo.Studies from the Institute for Medical Research,Malaysia.
Ronquist F,Huelsenbeck JP,2003.Mr Bayes 3:Bayesian phylogenetic inference under mixed models.Bioinformatics,19(12):1572-1574.
Sallum MAM,Schultz TR,Foster PG,Aronstein K,Wirtz RA,Wilkerson RC,2002.Phylogeny of Anophelinae(Diptera:Culicidae)based on nuclear ribosomal and mitochondrial DNAsequences.Syst.Entomol.,27(3):361-382.
Sallum MAM,Schultz TR,Wilkerson RC,2000.Phylogeny of Anophelinae(Diptera Culicidae)based on morphological characters.Ann.Entomol.Soc.Am.,93(4):745-775.
Sames WJ,Kim HC,Chong ST,Harrison BA,Lee WJ,Rueda LM,Klein TA,2008.Anopheles lindesayi japonicus Yamada(Diptera:Culicidae)in Korea:comprehensive review,new collection records,and description of larval habitats.J.Vector Ecol.,33(1):99-106.
Stamatakis A,2014.RAx ML version 8:a tool for phylogenetic analysis and post-analysis of large phylogenies.Bioinformatics,30(9):1312-1313.
Talavera G,Castresana J,2007.Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments.Syst.Biol.,56(4):564-577.
Tamura K,Peterson D,Peterson N,Stecher G,Nei M,Kumar S,2014.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods.Mol.Biol.Evol.,28(10):2731-2739.
Varani G,Mcclain WH,2000.The G x U wobble base pair.Afundamental building block of RNA structure crucial to RNAfunction in diverse biological systems.EMBO Rep.,1(1):18-23.
Wang G,Li C,Guo X,Xing D,Dong Y,Wang Z,Zhang Y,Liu M,Zheng Z,Zhang H,2012.Identifying the main mosquito species in China based on DNA barcoding.PLoS ONE,7(10):e47051.
Wang G,Li C,Guo X,Xing D,Dong Y,Zhao T,2014.Molecular phylogenetic analysis of the subgenera Anopheles and Cellia(Diptera:Culicidae)based on nuclear ribosomal sequences.Afr.Entomol.,22(3):660-669.
Wei SJ,Shi M,Sharkey MJ,Cornelis VA,Chen XX,2010.Comparative mitogenomics of Braconidae(Insecta:Hymenoptera)and the phylogenetic utility of mitochondrial genomes with special reference to holometabolous insects.BMC Genomics,11(1):371-386.
Zhang NX,Yu G,Li TJ,He QY,Zhou Y,Si FL,Ren S,Chen B,2015.The complete mitochondrial genome of Delia antiqua and its implications in dipteran phylogenetics.PLoS ONE,10(10):e0139736.
Zhang NX,Zhang YJ,Yu G,Chen B,2013.Structure characteristics of the mitochondrial genomes of Diptera and design and application of universal primers for their sequencing.Acta Entomol.Sin.,56(4):398-407.[张乃心,张玉娟,余果,陈斌,2013.双翅目昆虫线粒体基因组结构特点及其测序通用引物的设计和应用.昆虫学报,56(4):398-407]
Zhao L,Zheng ZM,Huang Y,Sun HM,2010.A comparative analysisof mitochondrial genomes in Orthoptera(Arthropoda:Insecta)andgenome descriptions of three grasshopper species.Zool.Sci.,27(8):662-672.
Beard CB,Hamm DM,Collins FH,1993.The mitochondrial genome of the mosquito Anopheles gambiae:DNA sequence,genome organization,and comparisons with mitochondrial sequences of other insects.Insect Mol.Biol.,2(2):103-124.
Boore JL,1999.Animal mitochondrial genomes.Nucleic Acids Res.,27(8):1767-1780.
Boore JL,2006.The use of genome-level characters for phylogenetic reconstruction.Trends Ecol.Evol.,21(8):439-446.
Boore JL,Macey JR,Medina M,2005.Sequencing and comparing whole mitochondrial genomes of animals.Methods Enzymol.,395:311-348.
Cameron SL,2014.Insect mitochondrial genomics:implications for evolution and phylogeny.Annu.Rev.Entomol.,59:95-117.
Demari-Silva B,Foster PG,Oliveira TMPD,Bergo ES,Sanabani SS,Pessoa R,Sallum MAM,2015.Mitochondrial genomes and comparative analyses of Culex camposi,Culex coronator,Culex usquatus,and Culex usquatissimus,(Diptera:Culicidae),members of the coronator group.BMC Genomics,16:831.
Foley DH,Bryan JH,Yeates D,Saul A,1998.Evolution and systematics of Anopheles:insights from a molecular phylogeny of Australasian mosquitoes.Mol.Phylogenet.Evol.,9(2):262-275.
Glick JI,1992.Illustrated key to the female Anopheles of southwestern Asia and Egypt(Diptera:Culicidae).Mosq.Syst.,24(2):125-153.
Gutell RR,Lee JC,Cannone JJ,2002.The accuracy of ribosomal RNAcomparative structure models.Curr.Opin.Struct.Biol.,12(3):301-310.
Hao YJ,Zou YL,Ding YR,Xu WY,Yan ZT,Li XD,Fu WB,Li TJ,Chen B,2017.Complete mitochondrial genomes of Anopheles stephensi and An.dirus and comparative evolutionary mitochondriomics of 50 mosquitoes.Sci.Rep.,7(1):7666-7679.
Harbach RE,2007.The Culicidae(Diptera):a review of taxonomy,classification and phylogeny.Zootaxa,1668:591-638.
Harbach RE,Kitching IJ,2005.Reconsideration of anopheline mosquito phylogeny(Diptera:Culicidae:Anophelinae)based on morphological data.Syst.Biodivers.,3(4):345-374.
Harbach RE,Kitching IJ,2010.Phylogeny and classification of the Culicidae(Diptera).Syst.Entomol.,23(4):327-370.
Harbach RE,Kitching IJ,2016.The phylogeny of Anophelinae revisited:inferences about the origin and classification of Anopheles(Diptera:Culicidae).Zool.Scr.,45(1):34-47.
Harrison BA,Rattanarithikul R,Peyton EL,Mongkolpanya K,1990.Taxonomic changes,revised occurrence records and notes on the Culicidae of Thailand and neighboring countries.Mosq.Syst.,22(3):196-227.
Hua YQ,Ding YR,Yan ZT,Si FL,Luo QC,Chen B,2016.The complete mitochondrial genome of Anopheles minimus(Diptera:Culicidae)and the phylogenetics of known Anopheles mitogenomes.Insect Sci.,23(3):353-365.
Hua YQ,Yan ZT,Fu WB,He QY,Zhou Y,Chen B,2015.Sequencing and analysis of the complete mitogenome in Anopheles culicifacies species B(Diptera:Culicidae).Mitochondr.DNA Part A,27(4):2909-2910.
Kazutaka K,Standley DM,2013.MAFFT multiple sequence alignment software version 7:improvements in performance and usability.Mol.Biol.Evol.,30(4):772-780.
Kim H,Sames WS,Lee I,Lee D,Kim H,Rueda L,Klein T,2009.Overwintering of Anopheles lindesayi japonicus larvae in the Republic of Korea.J.Am.Mosq.Control Assoc.,25(1):32-37.
Kim MJ,Wang AR,Park JS,Kim I,2014.Complete mitochondrial genomes of five skippers(Lepidoptera:Hesperiidae)and phylogenetic reconstruction of Lepidoptera.Gene,549(1):97-112.
Krzywinski J,Grushko OG,Besansky NJ,2006.Analysis of the complete mitochondrial DNA from Anopheles funestus:an improved dipteran mitochondrial genome annotation and a temporal dimension of mosquito evolution.Mol.Phylogenet.Evol.,39(2):417-423.
Krzywinski J,Li C,Morris M,Conn JE,Lima JB,Povoa MM,Wilkerson RC,2011.Analysis of the evolutionary forces shaping mitochondrial genomes of a Neotropical malaria vector complex.Mol.Phylogenet.Evol.,58(3):469-477.
Krzywinski J,Wilkerson RC,Besansky NJ,2001.Evolution of mitochondrial and ribosomal gene sequences in Anophelinae(Diptera:Culicidae):implications for phylogeny reconstruction.Mol.Phylogenet.Evol.,18(3):479-487.
Librado P,Rozas J,2009.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Logue K,Chan ER,Phipps T,Small ST,Reimer L,Henryhalldin C,Sattabongkot J,Siba PM,Zimmerman PA,Serre D,2013.Mitochondrial genome sequences reveal deep divergences among Anopheles punctulatus sibling species in Papua New Guinea.Malar.J.,12(1):64-74.
Lowe TM,Eddy SR,1997.t RNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence.Nucleic Acids Res.,25(5):955-964.
Luo QC,Hao YJ,Meng F,Li TJ,Ding YR,Hua YQ,Chen B,2016.The mitochondrial genomes of Culex tritaeniorhynchus and Culex pipiens pallens(Diptera:Culicidae)and comparison analysis with two other Culex species.Parasit.Vectors,9(1):406-416.
Ma Y,Ma YJ,Lin L,Wang Y,2013.Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles(Diptera:Culicidae)in China:based on r DNA-ITS2 sequences.Chin.J.Vector Biol.Control,24(5):382-388.[马颖,马雅军,林琳,王琰,2013.基于r DNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究.中国媒介生物学及控制杂志,24(5):382-388]
Masta SE,Boore JL,2004.The complete mitochondrial genome sequence of the spider Habronattus oregonensis reveals rearranged and extremely truncated t RNAs.Mol.Biol.Evol.,21(5):893-902.
Masta SE,Boore JL,2008.Parallel evolution of truncated transfer RNAgenes in arachnid mitochondrial genomes.Mol.Biol.Evol.,25(5):949-959.
Mitchell SE,Cockburn AF,Seawright JA,1993.The mitochondrial genome of Anopheles quadrimaculatus species A:complete nucleotide sequence and gene organization.Genome,36(6):1058-1073.
Mohanty A,Swain S,Kar SK,Hazra RK,2009.Analysis of the phylogenetic relationship of Anopheles species,subgenus Cellia(Diptera:Culicidae)and using it to define the relationship of morphologically similar species.Infect.Genet.Evol.,9(6):1204-1224.
Moreno M,Marinotti O,Krzywinski J,Tadei WP,James AA,Achee N,Conn JE,2010.Complete mt DNA genomes of Anopheles darlingi,and an approach to anopheline divergence time.Malaria J.,9(1):127-127.
Negrisolo,E,Babbucci M,Patarnello T,2011.The mitochondrial genome of the ascalaphid owlfly Libelloides macaronius and comparative evolutionary mitochondriomics of neuropterid insects.BMC Genomics,12(1):221-246.
Ojala D,Montoya J,Attardi G,1981.TRNA punctuation model of RNAprocessing in human mitochondrial.Nature,290(5806):470-474.
Oliveira TMP,Foster PG,Bergo ES,Nagaki SS,Sanabani SS,Marinotti O,Marinotti PN,Sallum MAM,2016.Mitochondrial genomes of Anopheles(Kerteszia)(Diptera:Culicidae)from the Atlantic forest,Brazil.J.Med.Entomol.,53(4):tjw001.
Perna NT,Kocher TD,1995.Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes.J.Mol.Evol.,41(3):353-358.
Posada D,Crandall KA,1998.MODELTEST,Ver.3.06:testing the model of DNA substitution.Bioinformatics,14(9):817-818.
Reid JA,1968.Anopheline mosquitoes of Malaya and Borneo.Studies from the Institute for Medical Research,Malaysia.
Ronquist F,Huelsenbeck JP,2003.Mr Bayes 3:Bayesian phylogenetic inference under mixed models.Bioinformatics,19(12):1572-1574.
Sallum MAM,Schultz TR,Foster PG,Aronstein K,Wirtz RA,Wilkerson RC,2002.Phylogeny of Anophelinae(Diptera:Culicidae)based on nuclear ribosomal and mitochondrial DNAsequences.Syst.Entomol.,27(3):361-382.
Sallum MAM,Schultz TR,Wilkerson RC,2000.Phylogeny of Anophelinae(Diptera Culicidae)based on morphological characters.Ann.Entomol.Soc.Am.,93(4):745-775.
Sames WJ,Kim HC,Chong ST,Harrison BA,Lee WJ,Rueda LM,Klein TA,2008.Anopheles lindesayi japonicus Yamada(Diptera:Culicidae)in Korea:comprehensive review,new collection records,and description of larval habitats.J.Vector Ecol.,33(1):99-106.
Stamatakis A,2014.RAx ML version 8:a tool for phylogenetic analysis and post-analysis of large phylogenies.Bioinformatics,30(9):1312-1313.
Talavera G,Castresana J,2007.Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments.Syst.Biol.,56(4):564-577.
Tamura K,Peterson D,Peterson N,Stecher G,Nei M,Kumar S,2014.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods.Mol.Biol.Evol.,28(10):2731-2739.
Varani G,Mcclain WH,2000.The G x U wobble base pair.Afundamental building block of RNA structure crucial to RNAfunction in diverse biological systems.EMBO Rep.,1(1):18-23.
Wang G,Li C,Guo X,Xing D,Dong Y,Wang Z,Zhang Y,Liu M,Zheng Z,Zhang H,2012.Identifying the main mosquito species in China based on DNA barcoding.PLoS ONE,7(10):e47051.
Wang G,Li C,Guo X,Xing D,Dong Y,Zhao T,2014.Molecular phylogenetic analysis of the subgenera Anopheles and Cellia(Diptera:Culicidae)based on nuclear ribosomal sequences.Afr.Entomol.,22(3):660-669.
Wei SJ,Shi M,Sharkey MJ,Cornelis VA,Chen XX,2010.Comparative mitogenomics of Braconidae(Insecta:Hymenoptera)and the phylogenetic utility of mitochondrial genomes with special reference to holometabolous insects.BMC Genomics,11(1):371-386.
Zhang NX,Yu G,Li TJ,He QY,Zhou Y,Si FL,Ren S,Chen B,2015.The complete mitochondrial genome of Delia antiqua and its implications in dipteran phylogenetics.PLoS ONE,10(10):e0139736.
Zhang NX,Zhang YJ,Yu G,Chen B,2013.Structure characteristics of the mitochondrial genomes of Diptera and design and application of universal primers for their sequencing.Acta Entomol.Sin.,56(4):398-407.[张乃心,张玉娟,余果,陈斌,2013.双翅目昆虫线粒体基因组结构特点及其测序通用引物的设计和应用.昆虫学报,56(4):398-407]
Zhao L,Zheng ZM,Huang Y,Sun HM,2010.A comparative analysisof mitochondrial genomes in Orthoptera(Arthropoda:Insecta)andgenome descriptions of three grasshopper species.Zool.Sci.,27(8):662-672.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |