淡色库蚊对苏云金杆菌及化学杀虫剂的抗性研究
|
摘要
目的探讨苏云金杆菌以色列亚种(Bti)与常用化学杀虫剂联合使用对东平湖地区淡色库蚊幼虫的杀灭效果。方法采用幼虫浸渍法测试现场淡色库蚊幼虫对Bti及5种化学杀虫剂的抗性,采用简单复配法检测Bti分别与5种化学杀虫剂混用的增效效果,增效明显的2组再次进行复配验证。结果现场淡色库蚊幼虫对Bti、溴氰菊酯、氯氰菊酯、敌敌畏、残杀威、三氯杀虫酯的抗性倍数分别为1. 04、106. 00、83. 00、5. 03、3. 51、1. 01。Bti分别与5种化学杀虫剂简单复配时均有增效效果,Bti+残杀威增效效果最佳(19. 63),其次为Bti+氯氰菊酯(11. 59)。进一步复配结果显示,残杀威与Bti以不同比例复配时都具有增效作用,其中以1∶3比例复配时共毒系数最高(189);当氯氰菊酯与Bti以1∶1及1∶3比例复配时,表现出增效作用(共毒系数:114、135)。结论将Bti与化学杀虫剂联合应用(尤其是残杀威)对山东省东平湖地区淡色库蚊有较好的灭杀效果,可延缓抗药性的发展,是蚊虫抗性治理中较为重要的防治策略。
Objective To explore the effect of Bacillus thuringiensis subsp. israelensis( Bti) combined with common chemical insecticides on Culex pipiens pallens larvae in Dongping Lake area. Methods The larvae impregnation method was used to test the resistance of Culex pipiens pallens larvae to Bti and 5 chemical insecticides. The synergistic effect of Bti mixed with 5 chemical insecticides was tested by simple mix method,and the cotoxicity coefficients were determined. Results Culex pipiens pallens larvae evolved 1. 04,106. 00,83. 00,5. 03,3. 51 and1. 01 folds resistance respectively to Bti,deltamethrin,cypermethrin,dichlorvos,propoxur and acetofenate. The mixture of Bti with five chemical insecticides showed synergistic effect,best with propoxur( 19. 63),second with cypermethrin( 11. 59). The cotoxicity coefficient was the highest as much as 189,when Bti mixed with propoxur as 3∶ 1. When Bti mixed with cypermethrin as 1∶ 1 and 3∶ 1,the co-toxicity coefficients were 114 and 135. Conclusion The combination of Bti and chemical insecticides( especially propoxur) showed a good effect on Culex pipiens pallens in Dongping Lake area of Shandong Province,which might delay the development of insecticides resistance,showing importance in mosquito resistance management.
Objective To explore the effect of Bacillus thuringiensis subsp. israelensis( Bti) combined with common chemical insecticides on Culex pipiens pallens larvae in Dongping Lake area. Methods The larvae impregnation method was used to test the resistance of Culex pipiens pallens larvae to Bti and 5 chemical insecticides. The synergistic effect of Bti mixed with 5 chemical insecticides was tested by simple mix method,and the cotoxicity coefficients were determined. Results Culex pipiens pallens larvae evolved 1. 04,106. 00,83. 00,5. 03,3. 51 and1. 01 folds resistance respectively to Bti,deltamethrin,cypermethrin,dichlorvos,propoxur and acetofenate. The mixture of Bti with five chemical insecticides showed synergistic effect,best with propoxur( 19. 63),second with cypermethrin( 11. 59). The cotoxicity coefficient was the highest as much as 189,when Bti mixed with propoxur as 3∶ 1. When Bti mixed with cypermethrin as 1∶ 1 and 3∶ 1,the co-toxicity coefficients were 114 and 135. Conclusion The combination of Bti and chemical insecticides( especially propoxur) showed a good effect on Culex pipiens pallens in Dongping Lake area of Shandong Province,which might delay the development of insecticides resistance,showing importance in mosquito resistance management.
引文
[1] Luo QC,Hao YJ,Meng F,et al. The mitochondrial genomes of Culex tritaeniorhynchus and Culex pipiens pallens(Diptera:Culicidae)and comparison analysis with two other Culex species[J]. Parasit Vectors,2016,9(1):406.
[2] Rasic G,Schama R,Powell R,et al. Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro:implications for vector control[J].Evol Appl,2015,8(9):901-915.
[3]韩光杰,李传明,孙俊,等.苏云金杆菌以色列亚种对蚊幼虫体内解毒酶活性的影响[J].中国血吸虫病防治杂志,2015,27(4):385-389.
[4] Jakob C,Poulin B,Leather SR,et al. Indirect effects of mosquito control using Bti on dragonflies and damselflies(Odonata)in the Camargue[J]. Insect Conserv Diver,2016,9(2):161-169.
[5] Heimpel AM. A critical review of Bacillus thuringiensis var thuringiensis Berliner and other crystalliferous bacteria[J]. Annu Rev Entomol,1967,12:287-322.
[6]刘维德.蚊类抗药性及其测定[M].北京:科学出版社,1979:1-114.
[7]唐振华.杀虫剂混用的毒力测定[J].昆虫知识,1980,17(3):136-138.
[8] Guo J,Ye W,Liu X,et al. piRNA-3312:A putative role for pyrethroid resistance in Culex pipiens pallens(Diptera:Culicidae)[J]. J Med Entomol,2017,54(4):1013-1018.
[9] Benelli G,Lo Iacono A,Canale A,et al. Mosquito vectors and the spread of cancer:an overlooked connection?[J]. Parasitol Res,2016,115(6):2131-2137.
[10]江洪涛,王怀位,甄天民,等.山东省淡色库蚊抗药性调查及防制对策的研究[J].中国热带医学,2005,5(1):17-18.
[11]黄晓丹,赵久旭,寇景轩,等.山东省东平湖地区淡色库蚊对常用杀虫剂的抗药性调查[J].中国媒介生物学及控制杂志,2013,24(5):406-408.
[12] Ibrahim MA,Griko N,Junker M,et al. Bacillus thuringiensis:a genomics and proteomics perspective[J]. Bioeng Bugs,2010,1(1):31-50.
[13]关鹏,秦培钢,代小娟,等.苏云金芽胞杆菌cyt2Ba16基因的克隆表达[J].生物技术,2018,28(2):119.
[14] Morris ON,Converse V,Kanagaratnam P. Chemical additive effects on the efficacy of Bacillus thuringiensis berliner subsp. kurstaki against Mamestra configurata(Lepidoptera:Noctuidae)[J]. J Econ Entomol,1995,88(4):815-824.
[15]邱思鑫.苏云金芽孢杆菌高效菌株的选育、发酵培养与添加剂筛选[D].福州:福建农林大学,2001.
[2] Rasic G,Schama R,Powell R,et al. Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro:implications for vector control[J].Evol Appl,2015,8(9):901-915.
[3]韩光杰,李传明,孙俊,等.苏云金杆菌以色列亚种对蚊幼虫体内解毒酶活性的影响[J].中国血吸虫病防治杂志,2015,27(4):385-389.
[4] Jakob C,Poulin B,Leather SR,et al. Indirect effects of mosquito control using Bti on dragonflies and damselflies(Odonata)in the Camargue[J]. Insect Conserv Diver,2016,9(2):161-169.
[5] Heimpel AM. A critical review of Bacillus thuringiensis var thuringiensis Berliner and other crystalliferous bacteria[J]. Annu Rev Entomol,1967,12:287-322.
[6]刘维德.蚊类抗药性及其测定[M].北京:科学出版社,1979:1-114.
[7]唐振华.杀虫剂混用的毒力测定[J].昆虫知识,1980,17(3):136-138.
[8] Guo J,Ye W,Liu X,et al. piRNA-3312:A putative role for pyrethroid resistance in Culex pipiens pallens(Diptera:Culicidae)[J]. J Med Entomol,2017,54(4):1013-1018.
[9] Benelli G,Lo Iacono A,Canale A,et al. Mosquito vectors and the spread of cancer:an overlooked connection?[J]. Parasitol Res,2016,115(6):2131-2137.
[10]江洪涛,王怀位,甄天民,等.山东省淡色库蚊抗药性调查及防制对策的研究[J].中国热带医学,2005,5(1):17-18.
[11]黄晓丹,赵久旭,寇景轩,等.山东省东平湖地区淡色库蚊对常用杀虫剂的抗药性调查[J].中国媒介生物学及控制杂志,2013,24(5):406-408.
[12] Ibrahim MA,Griko N,Junker M,et al. Bacillus thuringiensis:a genomics and proteomics perspective[J]. Bioeng Bugs,2010,1(1):31-50.
[13]关鹏,秦培钢,代小娟,等.苏云金芽胞杆菌cyt2Ba16基因的克隆表达[J].生物技术,2018,28(2):119.
[14] Morris ON,Converse V,Kanagaratnam P. Chemical additive effects on the efficacy of Bacillus thuringiensis berliner subsp. kurstaki against Mamestra configurata(Lepidoptera:Noctuidae)[J]. J Econ Entomol,1995,88(4):815-824.
[15]邱思鑫.苏云金芽孢杆菌高效菌株的选育、发酵培养与添加剂筛选[D].福州:福建农林大学,2001.