一株磺化沥青降解菌的筛选鉴定及降解特性研究
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摘要
为了选育降解磺化钻井液的微生物菌株,以新疆克拉玛依油田磺化钻井液污泥为微生物菌源,磺化沥青为唯一碳源,进行富集、驯化、分离纯化等筛选有效降解磺化沥青的菌株,经生理生化及分子生物技术鉴定种名,单因素实验和正交实验研究降解特性。结果获得一株有效降解磺化沥青的菌株,命名为X2,生理生化与分子鉴定为大洋沉积物芽孢杆菌。单因素实验表明,X2生长最佳的P源为KH_2PO_4,N源为NH_4NO_3;正交实验结果显示,X2菌体最佳生长条件为温度为36℃、pH值为7.8、盐浓度为1%、接种量为10%;COD降解最佳条件为36℃、pH值为8.2、盐浓度为0.9%、接种量为12%,降解率为59%～71%;pH值的R极差最大,是同时影响菌株生长及COD降解的关键因子。实验结果为降解钻井废液COD提供了有效的理论基础。
To find out a microbial strain for effective degradation of sulfonate drilling fluid, experiments were conducted using drilling fluid sludge from Kelamayi Oilfield as the source of microbe and sulfonated asphalt as the sole carbon source for concentration,acclimation, isolation and purification. The species of the microbe was determined with physiological biochemistry technology and molecular biotechnology. The degradation behavior of the microbe was studied through single-factor experiment and orthogonal experiment. A microbial strain that is able to effectively and efficiently degrade sulfonated asphalt was found through these experiments,and was named X2, which is an Oceanobacillus(in ocean sediments) determined with physiological biochemistry and molecular characterization. Single-factor experiment showed that the best P-source for X2 to grow is KH_2PO_4, and the best N-source is NH_4NO_3.Orthogonal experiment showed that the optimum growing conditions for X2 are as follows: 36 ℃, pH 7.8, 1% salt and inoculum concentration of 10%. The optimum degradation conditions for X2 are as follows: 36 ℃, pH 8.2, 0.9% salt and inoculum concentration of 12%. The rate of degradation at these conditions is 59%-71%. The Range of the pH values is highest and is thus a key factor affecting both the growth of the strain and the COD for X2 to degrade. The experimental results have provided an effective theoretical basis for degrading waste drilling fluids.
To find out a microbial strain for effective degradation of sulfonate drilling fluid, experiments were conducted using drilling fluid sludge from Kelamayi Oilfield as the source of microbe and sulfonated asphalt as the sole carbon source for concentration,acclimation, isolation and purification. The species of the microbe was determined with physiological biochemistry technology and molecular biotechnology. The degradation behavior of the microbe was studied through single-factor experiment and orthogonal experiment. A microbial strain that is able to effectively and efficiently degrade sulfonated asphalt was found through these experiments,and was named X2, which is an Oceanobacillus(in ocean sediments) determined with physiological biochemistry and molecular characterization. Single-factor experiment showed that the best P-source for X2 to grow is KH_2PO_4, and the best N-source is NH_4NO_3.Orthogonal experiment showed that the optimum growing conditions for X2 are as follows: 36 ℃, pH 7.8, 1% salt and inoculum concentration of 10%. The optimum degradation conditions for X2 are as follows: 36 ℃, pH 8.2, 0.9% salt and inoculum concentration of 12%. The rate of degradation at these conditions is 59%-71%. The Range of the pH values is highest and is thus a key factor affecting both the growth of the strain and the COD for X2 to degrade. The experimental results have provided an effective theoretical basis for degrading waste drilling fluids.
引文
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[10]谢彬强,邱正松.无固相钻井液超高温增黏剂SDKP的结构、性能及应用[J].油田化学,2014,31(4):481-487.XIE Binqiang,QIU Zhengsong.Structure,properties and application of super high temperature tackifier SDKP for solid-free drilling fluid[J].Oilfield Chemistry,2014,31(4):481-487.
[11]刘音,崔远众,常青,等.油田钻井废液处理研究进展[J].石油化工应用,2014,33(10):1-5.LIU Yin,CUI Yuanzhong,CHANG Qing,et al.Research progress on treatment of drilling waste fluid[J].Petrochemical Industry Application,2014,33(10):1-5.
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[13]舒福昌,罗刚,张岩,等.废弃水基钻井液低压蒸馏处理技术研究与应用[J].石油天然气学报,2013,35(2):117-119.SHU Fuchang,LUO Gang,ZHANG Yan,et al.Research and application of low pressure distillation technology for waste water-based drilling fluid[J].Journal of Oil and Gas Technology,2013,35(2):117-119.
[14]刘宇程,徐俊忠,袁建梅,等.废弃油基钻井液热化学破乳-离心分离实验[J].环境工程学报,2013,7(6):2333-2338.LIU Yucheng,XU Junzhong,YUAN Jianmei,et al.Experiment on thermo-chemical demulsification and centrifugal separation of abandoned oil-based drilling fluid[J].Chinese Journal of Environmental Engineering,2013,7(6):2333-2338.
[15]陈明燕,徐琦,罗林.新疆油田钻井废液固液分离实验研究及现场应用[J].石油与天然气化工,2012,41(3):346-348.CHEN Mingyan,XU Qi,LUO Lin.Experimental research and field application of solid-liquid separation of drilling waste fluid in Xinjiang oilfield[J].Chemical Engineering of Oil&Gas,2012,41(3):346-348.
[16]胡友林,乌效鸣.江汉油田废弃油基钻井液处理[J].东北石油大学学报,2013,37(1):64-70.HU Youlin,WU Xiaoming.Treatment of waste oil-based drilling fluids in Jianghan oilfield[J].Journal of Northeast Petroleum University,2013,37(1):64-70.
[17]谢水祥,蒋官澄,陈勉,等.废弃油基钻井液资源回收与无害化处置[J].环境科学研究,2011,24(5):540-547.XIE Shuixiang JIANG Guancheng,CHEN Mian,et al.Resource recycling and harmless treatment for waste oil-based drilling fluid[J].Research of Environmental Sciences,2011,24(5):540-547.
[18]郭振英,吕荣湖,张红生,等.生物法处理磺化钻井液体系钻井废水的可行性初探[J].石油化工高等学校学报,2009,22(1):5-8.GUO Zhenying,LYU Ronghu,ZHANG Hongsheng,et al.Preliminary study on drilling wastewater correlated with three-sulfonated mud system treatment with aerobic process[J].Journal Of Petrochemical Universities,2009,22(1):5-8.
[19]仝坤,张以河,谢加才,等.提高稠油废水可生化性的研究进展[J].化工环保,2011,31(5):423-426.TONG Kun,ZHANG Yihe,XIE Jiacai,et al.Research progresses in biodegradability improvement of viscous oil wastewater[J].Environmental Protection of Chemical Industry,2011,31(5):423-426.
[20]霍丹群,秦力,肖灵铃,等.生物法处理含油钻井废水的影响因素研究[J].环境工程学报,2007,1(6):56-59.HUO Danqun,QIN Li,XIAO Lingling,et al.Factors in the treatment of drilling wastewater by biological me thod[J].Chine s e Journal of Env i ronmental Engineering,2007,1(6):56-59.
[21]肖灵铃,霍丹群,秦力,等.微生物法处理钻井废水中的石油污染物[J].工业水处理,2006,26(4):59-65.XIAO Lingling,HUO Danqun,QIN Li,et al.Treatment of petroleum pollutants from drilling wastewater by microbiological method[J].Industrial Water Treatment,2006,26(4):59-65.
[22]汪严明,杨敏,郑少奎,等.用酵母菌处理油田钻井废水的研究[J].环境科学,2002,23(5):72-75.WANG Yanming,YANG Min,ZHEN Shaokui,et al.Treatment of drilling wastewater from oil field by using yeast[J].Environmental Science,2002,23(5):72-75.
[23]张淑侠,何焕杰,吕宁超,等.复合菌剂处理深井聚磺钻井液废水技术研究[J].西安石油大学学报,2012,27(6):48-52.ZHANG Shuxia,HE Huanjie,LYU Ningchao,et al.Study on treatment of deep well polysulfonated drilling fluid wastewater by compound bacteria agent[J].Journal of Xi'an Shiyou University(Natural Science Edition),2012,27(6):48-52.
[24]冯栩,谷晋川,鲍晋,等.钻井废水的生物强化处理[J].环境工程学报,2014,8(1):184-189.F ENG Xu,GU Jinchuan,BAO Jin,et al.Bioaugmentation treatment of drilling wastewater[J].Chinese Journal of Environmental Engineering,2014,8(1):184-189.
[25]宋淑芬,马立安,胡传炯,等.一株磺化钻井液降解菌的筛选鉴定及其降解特性[J].石油钻采工艺,2018,40(5):589-595.SONG Shufen,MA Lian,HU Chuanjiong,et al.Screening,evaluation and degradation property of the degrading bacteria suitable for sulphonated drilling fluid[J].Oil Drilling&Production Technology,2018,40(5):589-595.
[2]蔡利山.石油钻井废弃物环境污染特征的分析与评价[J].西部探矿工,2003,15(2):54-57.CAI Lishan.Analysis and evaluation of environmental pollution characteristics of oil drilling waste[J].WestChina Exploration Engineering,2003,15(2):54-57.
[3]赵雄虎,王风春.废弃钻井液处理研究进展[J].钻井液与完井液,2004,21(2):43-48.ZHAO Xionghu,WANG Chunfeng.Research progress in waste drilling fluid treatment[J].Drilling Fluid&Completion Fluid,2004,21(2):43-48.
[4]张红岩,吕荣湖,郭绍辉.混凝-臭氧氧化法处理三磺钻井液体系钻井废水[J].过程工程学报,2007,7(4):718-722.ZHANG Hongyan,LYU Ronghu,GUO Shaohui.Treatment of drilling wastewater from trisulfonate mud system by coagulation-ozone oxidation process[J].The Chinese Journal of Process Engineering,2007,7(4):718-722.
[5]陈金照.磺化沥青钻井液在煤田地质钻探中的应用研究[J].能源与环境,2006,(5):109-110.CHEN Jinzhao.Application of sulfonated asphalt slurry in coalfield geological drilling[J].Energy and Environment,2006,(5):109-110.
[6]李勇,陈怡,王虎,等.磺化沥青钻井液在贵州地热勘探井中的应用[J].探矿工程(岩土钻掘工程),2015,42(1):27-30.LI Yong,CHEN Yi,WANG Hu,et al.Application of sulfonated asphalt drilling fluid in geothermal exploration well of Guizhou[J].Exploration Engineering(Rock&Soil Drilling and Tunneling),2015,42(1):27-30.
[7]ARGILLIER J F,SAINTPERE S,HERZHAFT B.Stability and flowing properties of aqueous foams for underbalanced drilling[J].SPE,48982,1998.
[8]Gulf Publishing Company.Classifications of fluid systems[J].World Oil 1998,219(6):106.
[9]黄维安,邱正松,乔军,等.抗温抗盐聚合物降滤失剂的研制及其作用机制[J].西南石油大学学报(自然科学版),2013,35(1):129-134.HUANG Wei'an,QIU Zhengsong,QIAO Jun,et al.Development of anti high temperature and salt polymer fluid loss agent and study on its operation mechanisms[J].Journal of Southwest Petroleum University(Science&Technology Edition),2013,35(1):129-134.
[10]谢彬强,邱正松.无固相钻井液超高温增黏剂SDKP的结构、性能及应用[J].油田化学,2014,31(4):481-487.XIE Binqiang,QIU Zhengsong.Structure,properties and application of super high temperature tackifier SDKP for solid-free drilling fluid[J].Oilfield Chemistry,2014,31(4):481-487.
[11]刘音,崔远众,常青,等.油田钻井废液处理研究进展[J].石油化工应用,2014,33(10):1-5.LIU Yin,CUI Yuanzhong,CHANG Qing,et al.Research progress on treatment of drilling waste fluid[J].Petrochemical Industry Application,2014,33(10):1-5.
[12]崔盈贤,唐晓东,陈信,等.钻井废水处理新工艺的研究与评价[J].工业水处理,2008,28(12):56-58.CUI Yingxian,TANG Xiaodong,CHEN Xin,et al.Research and evaluation of the new technology of well drilling wastewater treatment[J].Industrial Water Treatment,2008,28(12):56-58.
[13]舒福昌,罗刚,张岩,等.废弃水基钻井液低压蒸馏处理技术研究与应用[J].石油天然气学报,2013,35(2):117-119.SHU Fuchang,LUO Gang,ZHANG Yan,et al.Research and application of low pressure distillation technology for waste water-based drilling fluid[J].Journal of Oil and Gas Technology,2013,35(2):117-119.
[14]刘宇程,徐俊忠,袁建梅,等.废弃油基钻井液热化学破乳-离心分离实验[J].环境工程学报,2013,7(6):2333-2338.LIU Yucheng,XU Junzhong,YUAN Jianmei,et al.Experiment on thermo-chemical demulsification and centrifugal separation of abandoned oil-based drilling fluid[J].Chinese Journal of Environmental Engineering,2013,7(6):2333-2338.
[15]陈明燕,徐琦,罗林.新疆油田钻井废液固液分离实验研究及现场应用[J].石油与天然气化工,2012,41(3):346-348.CHEN Mingyan,XU Qi,LUO Lin.Experimental research and field application of solid-liquid separation of drilling waste fluid in Xinjiang oilfield[J].Chemical Engineering of Oil&Gas,2012,41(3):346-348.
[16]胡友林,乌效鸣.江汉油田废弃油基钻井液处理[J].东北石油大学学报,2013,37(1):64-70.HU Youlin,WU Xiaoming.Treatment of waste oil-based drilling fluids in Jianghan oilfield[J].Journal of Northeast Petroleum University,2013,37(1):64-70.
[17]谢水祥,蒋官澄,陈勉,等.废弃油基钻井液资源回收与无害化处置[J].环境科学研究,2011,24(5):540-547.XIE Shuixiang JIANG Guancheng,CHEN Mian,et al.Resource recycling and harmless treatment for waste oil-based drilling fluid[J].Research of Environmental Sciences,2011,24(5):540-547.
[18]郭振英,吕荣湖,张红生,等.生物法处理磺化钻井液体系钻井废水的可行性初探[J].石油化工高等学校学报,2009,22(1):5-8.GUO Zhenying,LYU Ronghu,ZHANG Hongsheng,et al.Preliminary study on drilling wastewater correlated with three-sulfonated mud system treatment with aerobic process[J].Journal Of Petrochemical Universities,2009,22(1):5-8.
[19]仝坤,张以河,谢加才,等.提高稠油废水可生化性的研究进展[J].化工环保,2011,31(5):423-426.TONG Kun,ZHANG Yihe,XIE Jiacai,et al.Research progresses in biodegradability improvement of viscous oil wastewater[J].Environmental Protection of Chemical Industry,2011,31(5):423-426.
[20]霍丹群,秦力,肖灵铃,等.生物法处理含油钻井废水的影响因素研究[J].环境工程学报,2007,1(6):56-59.HUO Danqun,QIN Li,XIAO Lingling,et al.Factors in the treatment of drilling wastewater by biological me thod[J].Chine s e Journal of Env i ronmental Engineering,2007,1(6):56-59.
[21]肖灵铃,霍丹群,秦力,等.微生物法处理钻井废水中的石油污染物[J].工业水处理,2006,26(4):59-65.XIAO Lingling,HUO Danqun,QIN Li,et al.Treatment of petroleum pollutants from drilling wastewater by microbiological method[J].Industrial Water Treatment,2006,26(4):59-65.
[22]汪严明,杨敏,郑少奎,等.用酵母菌处理油田钻井废水的研究[J].环境科学,2002,23(5):72-75.WANG Yanming,YANG Min,ZHEN Shaokui,et al.Treatment of drilling wastewater from oil field by using yeast[J].Environmental Science,2002,23(5):72-75.
[23]张淑侠,何焕杰,吕宁超,等.复合菌剂处理深井聚磺钻井液废水技术研究[J].西安石油大学学报,2012,27(6):48-52.ZHANG Shuxia,HE Huanjie,LYU Ningchao,et al.Study on treatment of deep well polysulfonated drilling fluid wastewater by compound bacteria agent[J].Journal of Xi'an Shiyou University(Natural Science Edition),2012,27(6):48-52.
[24]冯栩,谷晋川,鲍晋,等.钻井废水的生物强化处理[J].环境工程学报,2014,8(1):184-189.F ENG Xu,GU Jinchuan,BAO Jin,et al.Bioaugmentation treatment of drilling wastewater[J].Chinese Journal of Environmental Engineering,2014,8(1):184-189.
[25]宋淑芬,马立安,胡传炯,等.一株磺化钻井液降解菌的筛选鉴定及其降解特性[J].石油钻采工艺,2018,40(5):589-595.SONG Shufen,MA Lian,HU Chuanjiong,et al.Screening,evaluation and degradation property of the degrading bacteria suitable for sulphonated drilling fluid[J].Oil Drilling&Production Technology,2018,40(5):589-595.
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