无患子总皂苷的还原法脱色工艺研究
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摘要
采用还原法对深棕黄色的无患子总皂苷进行脱色处理,对关键的脱色工艺参数进行优化,得到的最佳工艺参数为:复合还原剂硼氢化钾和亚硫酸氢钠的添加质量分数均为4%,p H值为7,脱色温度为70℃、脱色时间为40 min,在该条件下脱色率为(72. 69±3. 34)%.采用紫外全波长扫描和黄嘌呤氧化酶体外抑制活性检测对脱色处理后的无患子总皂苷进行评价,结果表明:无患子皂苷经还原法脱色处理后,紫外吸收没有发生明显的变化,黄嘌呤氧化酶抑制活性与脱色前相当.这说明还原法脱色处理工艺对无患子总皂苷具有较好的脱色效果,并且对其理化性质和生物活性没有造成明显的影响.研究结果为无患子总皂苷在医药及日用化工行业中的高效利用提供了理论依据.
Sapindus mukorossi pericarps are rich in saponins,which are widely applicated in the industrials of pharmaceutical,cosmetic and some other fields due to its varieties of biological activities. However,the color of total saponins prepared by conventional methods is relatively dark,which limits their application in some products. The reduction method is employed to decolorize the color of the toptal saponins from Sapindus mukorossi pericarps. The decolorization reaches at( 72. 69 ± 3. 34) % with the following condition: the dosage of KBH4 and NaHSO3is 4%,4%respectively,pH of 7 within 70 ℃ for 40 min. The properties of Sapindus pericarps saponins before and after decolorizing are compared using the ultraviolet full-wavelength scanning and evaluated their inhibitory activity on xanthine oxidase in vitro,the results show that the saponins after decolorization still maintain the characteristics in ultraviolet absorption and a considerable inhibition of xanthine oxidase activity before decolorization. The present research results show a satisfactory decolorizing effect by reduction method and a not obvious change on its physicochemical properties and biological activities. This study provides a fundament for further development of saponins in pharmaceutical and cosmetic fields.
Sapindus mukorossi pericarps are rich in saponins,which are widely applicated in the industrials of pharmaceutical,cosmetic and some other fields due to its varieties of biological activities. However,the color of total saponins prepared by conventional methods is relatively dark,which limits their application in some products. The reduction method is employed to decolorize the color of the toptal saponins from Sapindus mukorossi pericarps. The decolorization reaches at( 72. 69 ± 3. 34) % with the following condition: the dosage of KBH4 and NaHSO3is 4%,4%respectively,pH of 7 within 70 ℃ for 40 min. The properties of Sapindus pericarps saponins before and after decolorizing are compared using the ultraviolet full-wavelength scanning and evaluated their inhibitory activity on xanthine oxidase in vitro,the results show that the saponins after decolorization still maintain the characteristics in ultraviolet absorption and a considerable inhibition of xanthine oxidase activity before decolorization. The present research results show a satisfactory decolorizing effect by reduction method and a not obvious change on its physicochemical properties and biological activities. This study provides a fundament for further development of saponins in pharmaceutical and cosmetic fields.
引文
[1]徐凯节,次旦扎西,丁立生.无患子属植物的化学成分及生物活性研究进展[J].天然产物研究与开发,2013,25(2):267-273.
[2]Sun Caowen,Wang Jiawei,Duan Jie,et al. Association of fruit and seed traits of Sapindus Mukorossi Germplasm with environmental factors in Southern China[J]. Forests,2017,8(12):491-506.
[3]Sonawane S M,Sonawane H. A review of recent and current research studies on the biological and pharmalogical activities of Sapindus Mukorossi[J]. International Journal of Interdisciplinary Research and Innovations,2015,3(4):85-95.
[4]Pradhan A,Bhattacharyya A. Quest for an eco-friendly alternative surfactant:surface and foam characteristics of natural surfactants[J]. Journal of Cleaner Production,2017,150:127-134.
[5]Samal K,Das C,Mohanty K. Eco-friendly biosurfactant saponin for the solubilization of cationic and anionic dyes in aqueous system[J]. Dyes and Pigments,2017,140:100-108.
[6]Samal K,Das C,Mohanty K. Application of saponin biosurfactant and its recovery in the MEUF process for removal of methyl violet from wastewater[J]. Journal of Environmental Management,2017,203:8-16.
[7]Sachin G,Dileep K,Gopal M,et al. Medicinal plants of the genus sapindus(sapindaceae):a review of their botany,phytochemisty,biological activity and traditional uses[J]. Journal of Drug Delivery and Therapeutics,2014,4(5):7-20.
[8]李林风,魏冰,邱立明,等.几种茶皂素脱色工艺的研究进展[J].粮食与食品工业,2017,24(1):57-58.
[9]程莉,龚耀刚,安凯,等.大孔树脂在三七叶皂苷脱色中的应用研究[J].云南中医学院学报,2005,28(3):13-14.
[10]任慧贤,王成忠,张代国.红曲霉发酵产物的脱色[J].中国酿造,2011,30(2):131-133.
[11]钟海雁,王承南,谢碧霞,等.茶油色泽测定及脱色工艺的研究[J].中南林业科技大学学报,2000,20(4):25-29.
[12]K9se M D,Bayraktar O. Extraction of saponins from soapnut(Sapindus mukorossi)and their antimicrobial properties[J]. World Journal of Research and Review,2016,2(5):89-93.
[13]Li Rui,Wu Zhaoliang,Wang Yanji,et al. Separation of total saponins from the pericarp of Sapindus mukorossi Gaerten. by foam fractionation[J]. Industrial Crops and Products,2013,51(6):163-170.
[14]Abdullahi A,Hamzah R U,Jigam A A,et al. Inhibitory activity of xanthine oxidase by fractions Crateva adansonii[J]. Journal of Acute Disease,2012,1(2):126-129.
[15] Akram M,Usmanghani K,Ahmed I,et al. Comprehensive review on therapeutic strategies of gouty arthritis[J]. Pakistan Journal of Pharmaceutical Sciences,2014,27(5):1575-1582.
[16]Azmi S M N,Jamal P,Amid A. Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout[J]. International Food Research Journal,2012,19(1):159-165.
[17]Ferraz Filha Z S,Vitolo I F,Fietto L G,et al. Xanthine oxidase inhibitory activity of Lychnophora species from Brazil("Arnica")[J]. Journal of Ethnopharmacology,2006,107(1):79-82.
[18]丁艳华,李海燕,杨俊,等.活性艳红水溶液的光助还原脱色反应机理研究[J].环境科学与技术,2009,32(12):37-40.
[19]程轶群,杜先锋.茶皂素还原脱色法初探[J].食品工业科技,2014,35(3):211-215.
[20]程轶群,赵敬娟,夏涛,等.茶皂素的陶瓷膜纯化工艺优化及脱色研究[J].农产品加工学刊,2011(11):11-15.
[21]曹湛慧,杨志娟,杨冰莲.橙子皮的脱色工艺条件的优化[J].食品工业,2015(9):109-112.
[22]程轶群.油茶皂苷的纯化及脱色工艺研究[D].合肥:安徽农业大学,2012.
[23]Wang Na,Chu Yanliang,Wu Fu'an,et al. Decolorization and degradation of Congo red by a newly isolated white rot fungus,Ceriporia lacerata,from decayed mulberry branches[J]. International Biodeterioration and Biodegradation,2017,117:236-244.
[24]Nagao A,Seki M,Kobayashi H. Inhibition of xanthine oxidase by flavonoids[J]. Bioscience,Biotechnology and Biochemistry,1999,63(10):2173-2176.
[25] Muhammad M T,Khan M N. Eco-friendly,biodegradable natural surfactant(Acacia Concinna):an alternative to the synthetic surfactants[J]. Journal of Cleaner Production,2018,188:678-685.
[26]Mondal M H,Malik S,Garain A,et al. Extraction of natural surfactant saponin from soapnut(Sapindus mukorossi)and its utilization in the remediation of hexavalent chromium from contaminated water[J]. Tenside Surfactants Detergents,2017,54(6):519-529.
[2]Sun Caowen,Wang Jiawei,Duan Jie,et al. Association of fruit and seed traits of Sapindus Mukorossi Germplasm with environmental factors in Southern China[J]. Forests,2017,8(12):491-506.
[3]Sonawane S M,Sonawane H. A review of recent and current research studies on the biological and pharmalogical activities of Sapindus Mukorossi[J]. International Journal of Interdisciplinary Research and Innovations,2015,3(4):85-95.
[4]Pradhan A,Bhattacharyya A. Quest for an eco-friendly alternative surfactant:surface and foam characteristics of natural surfactants[J]. Journal of Cleaner Production,2017,150:127-134.
[5]Samal K,Das C,Mohanty K. Eco-friendly biosurfactant saponin for the solubilization of cationic and anionic dyes in aqueous system[J]. Dyes and Pigments,2017,140:100-108.
[6]Samal K,Das C,Mohanty K. Application of saponin biosurfactant and its recovery in the MEUF process for removal of methyl violet from wastewater[J]. Journal of Environmental Management,2017,203:8-16.
[7]Sachin G,Dileep K,Gopal M,et al. Medicinal plants of the genus sapindus(sapindaceae):a review of their botany,phytochemisty,biological activity and traditional uses[J]. Journal of Drug Delivery and Therapeutics,2014,4(5):7-20.
[8]李林风,魏冰,邱立明,等.几种茶皂素脱色工艺的研究进展[J].粮食与食品工业,2017,24(1):57-58.
[9]程莉,龚耀刚,安凯,等.大孔树脂在三七叶皂苷脱色中的应用研究[J].云南中医学院学报,2005,28(3):13-14.
[10]任慧贤,王成忠,张代国.红曲霉发酵产物的脱色[J].中国酿造,2011,30(2):131-133.
[11]钟海雁,王承南,谢碧霞,等.茶油色泽测定及脱色工艺的研究[J].中南林业科技大学学报,2000,20(4):25-29.
[12]K9se M D,Bayraktar O. Extraction of saponins from soapnut(Sapindus mukorossi)and their antimicrobial properties[J]. World Journal of Research and Review,2016,2(5):89-93.
[13]Li Rui,Wu Zhaoliang,Wang Yanji,et al. Separation of total saponins from the pericarp of Sapindus mukorossi Gaerten. by foam fractionation[J]. Industrial Crops and Products,2013,51(6):163-170.
[14]Abdullahi A,Hamzah R U,Jigam A A,et al. Inhibitory activity of xanthine oxidase by fractions Crateva adansonii[J]. Journal of Acute Disease,2012,1(2):126-129.
[15] Akram M,Usmanghani K,Ahmed I,et al. Comprehensive review on therapeutic strategies of gouty arthritis[J]. Pakistan Journal of Pharmaceutical Sciences,2014,27(5):1575-1582.
[16]Azmi S M N,Jamal P,Amid A. Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout[J]. International Food Research Journal,2012,19(1):159-165.
[17]Ferraz Filha Z S,Vitolo I F,Fietto L G,et al. Xanthine oxidase inhibitory activity of Lychnophora species from Brazil("Arnica")[J]. Journal of Ethnopharmacology,2006,107(1):79-82.
[18]丁艳华,李海燕,杨俊,等.活性艳红水溶液的光助还原脱色反应机理研究[J].环境科学与技术,2009,32(12):37-40.
[19]程轶群,杜先锋.茶皂素还原脱色法初探[J].食品工业科技,2014,35(3):211-215.
[20]程轶群,赵敬娟,夏涛,等.茶皂素的陶瓷膜纯化工艺优化及脱色研究[J].农产品加工学刊,2011(11):11-15.
[21]曹湛慧,杨志娟,杨冰莲.橙子皮的脱色工艺条件的优化[J].食品工业,2015(9):109-112.
[22]程轶群.油茶皂苷的纯化及脱色工艺研究[D].合肥:安徽农业大学,2012.
[23]Wang Na,Chu Yanliang,Wu Fu'an,et al. Decolorization and degradation of Congo red by a newly isolated white rot fungus,Ceriporia lacerata,from decayed mulberry branches[J]. International Biodeterioration and Biodegradation,2017,117:236-244.
[24]Nagao A,Seki M,Kobayashi H. Inhibition of xanthine oxidase by flavonoids[J]. Bioscience,Biotechnology and Biochemistry,1999,63(10):2173-2176.
[25] Muhammad M T,Khan M N. Eco-friendly,biodegradable natural surfactant(Acacia Concinna):an alternative to the synthetic surfactants[J]. Journal of Cleaner Production,2018,188:678-685.
[26]Mondal M H,Malik S,Garain A,et al. Extraction of natural surfactant saponin from soapnut(Sapindus mukorossi)and its utilization in the remediation of hexavalent chromium from contaminated water[J]. Tenside Surfactants Detergents,2017,54(6):519-529.
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