栀子豉汤及拆方对六种人肠道菌的影响
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摘要
目的研究栀子豉汤及栀子、淡豆豉对6种人肠道菌的影响。方法从特定人的粪便中分离培养肠杆菌、肠球菌、双歧杆菌、乳杆菌、产气荚膜梭菌和脆弱拟杆菌。分别将高、中、低浓度的栀子、淡豆豉及栀子豉汤药液加入选择性培养基中,以不加药液的培养基作为空白对照,将实验菌分别于厌氧或有氧条件下培养,计数菌落。结果不同浓度的栀子对肠杆菌的抑制率均为100.0%,淡豆豉在20%、50%、70%浓度分别使肠杆菌数量下调100.00%、53.83%和22.11%,而栀子豉汤可使肠杆菌数量维持在一定水平(104～106 CFU/g);两味药单独都表现了对肠球菌较强的抑制作用,而低、中浓度栀子豉汤则使肠球菌保持较稳定的数量;单味药及复方对产气荚膜梭菌和双歧杆菌敏感性较弱;栀子对脆弱拟杆菌抑制作用最强,而淡豆豉和栀子豉汤则可使其数量上调约20.00%;淡豆豉、栀子豉汤和高浓度栀子对乳杆菌非常敏感,低、中浓度栀子对乳杆菌数量仅有轻微下调作用。结论栀子豉汤比单独的栀子和淡豆豉更能维持肠道菌群的相对平衡。
Objective To explore the effects of Gardenia jasminoides fruit(GJF),Sojae semen preparatum(SSP)and Zhizi-Chi Decoction(ZCD)on six resident bacteria in human gut.Methods The six gut bacteria(Enterobacter,Enterococcus,Bifidobacterium,Lactobacillus,Clostridium perfringens and Bacteroides)were isolated from the faeces of specific individual.Different concentrations of the aqueous extracts of GJF,SSP and ZCD were added to the selective media respectively;the media which did not contain the extracts were used as the blank controls.Then the experimental strains were cultured under anaerobic or aerobic conditions respectively,and the number of colony was counted.Results The inhibition rates of GJF to Enterobacter were 100.00%,regardless of the concentration;the inhibition rates of SSP were 100.00%,53.83% and22.11%at the concentration of 20%,50%and 70%,respectively;however,ZCD maintained the counts of Enterobacterat a level of 104-106 CFU/g.Both GJF and SSP exhibited higher sensitivity to Enterococcus,while ZCD at low and medium concentrations maintained a stable proportion of Enterococcus.The sensitivities of GJF,SSP and ZCD to Clostridium perfringens and Bifidobacteriumwere weak;the inhibition activity of GJF to Bacteroides was the highest,while SSP and ZCD increased the counts of Bacteroides by about 20.00%.SSP,ZCD and high concentration of GJF were especially sensitive to Lactobacillus,however,ZCD at low and medium concentrations showed only a slight down-regulative effect on the bacteria.Conclusion ZCD is superior to GJF and SSP for maintaining the relative balance of gut microbiota.
Objective To explore the effects of Gardenia jasminoides fruit(GJF),Sojae semen preparatum(SSP)and Zhizi-Chi Decoction(ZCD)on six resident bacteria in human gut.Methods The six gut bacteria(Enterobacter,Enterococcus,Bifidobacterium,Lactobacillus,Clostridium perfringens and Bacteroides)were isolated from the faeces of specific individual.Different concentrations of the aqueous extracts of GJF,SSP and ZCD were added to the selective media respectively;the media which did not contain the extracts were used as the blank controls.Then the experimental strains were cultured under anaerobic or aerobic conditions respectively,and the number of colony was counted.Results The inhibition rates of GJF to Enterobacter were 100.00%,regardless of the concentration;the inhibition rates of SSP were 100.00%,53.83% and22.11%at the concentration of 20%,50%and 70%,respectively;however,ZCD maintained the counts of Enterobacterat a level of 104-106 CFU/g.Both GJF and SSP exhibited higher sensitivity to Enterococcus,while ZCD at low and medium concentrations maintained a stable proportion of Enterococcus.The sensitivities of GJF,SSP and ZCD to Clostridium perfringens and Bifidobacteriumwere weak;the inhibition activity of GJF to Bacteroides was the highest,while SSP and ZCD increased the counts of Bacteroides by about 20.00%.SSP,ZCD and high concentration of GJF were especially sensitive to Lactobacillus,however,ZCD at low and medium concentrations showed only a slight down-regulative effect on the bacteria.Conclusion ZCD is superior to GJF and SSP for maintaining the relative balance of gut microbiota.
引文
[1]熊曼琪.伤寒学[M].2版.北京:中国中医药出版社,2010:85.
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[9] WANG Xiuhai.Preparation and pharmacodynamics studies on ZhiZiChiTangoral liquid[D].Harbin:Heilongjiang University of Chinese Medicine,2011.(in Chinese)王秀海.栀子豉汤口服液制备工艺及药效学研究[D].哈尔滨:黑龙江中医药大学,2011.
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[12] XIAO Hongbin,LIU Yanqiu,WANG Li,et al.Study on multi-component combination of Chinese prescriptions based on component interaction[J].World Sci Technol/Mod TCM&Materia Medica,2011,13(2):240-243.(in Chinese)肖红斌,刘艳秋,王莉,等.基于成分相互作用的中药复方组分配伍研究[J].世界科学技术·中医药现代化思路与方法,2011,13(2):240-243.
[13]杨化冰,邹小娟,孔明望,等.肠道微生态与传统中医思想内涵[J].中医杂志,2017,58(12):1070-1071.
[14]张季阶,于丽娴,徐灵芝,等.一株无毒脆弱拟杆菌的分离鉴定[J].宁夏医学杂志,1991,4(8):216-218.
[15]朱延旭,尚尔彬,王占红.BF-839脆弱拟杆菌对肉仔鸡生产性能的影响[J].现代畜牧兽医,2012(3):40-42.
[16] FENG Shuzhen,ZHANG Heiping.The research progress of Bacteroides fragilis[J].Microbiol China,2015,42(7):1366-1371.(in Chinese)冯淑贞,张和平.脆弱拟杆菌的研究进展[J].微生物学通报,2015,42(7):1366-1371.
[17] Salzman NH.The role of the microbiome in immune cell development[J]. Allergy Asthma Immunol,2014,113(6):293-298.
[18] Hsiao EY,McBride SW,Hsien S,et al.The microbiota modulates gut physiology and behavioral abnormalities associated with autism[J].Cell,2013,155(7):1451-1463.
[19] GAO Fang.Experimental study on treating the depression with decoction of capejasmine and fermented soybean[D].Fuzhou:FuJian University of Traditional Chinese Medicine,2007.(in Chinese)高芳.栀子豉汤治疗抑郁症的实验研究[D].福州:福建中医学院,2007.
[20]袁圣龙.栀子豉汤在心身疾病治疗中的应用[J].云南中医中药杂志,2013,34(6):76-77.
[21] Groen RN,de Clercq NC,Nieuwdorp M,et al.Gut microbiota,metabolism and psychopathology:A critical review and novel perspectives[J].Crit Rev Clin Lab Sci,2018,55(4):283-293.
[2]范越,田明,王秀海,等.栀子豉汤临床和实验研究进展[J].中医药学报,2010,38(1):118-119.
[3]苏悦,彭杨芷.栀子豉汤临床应用初探[J].亚太传统医药,2016,12(4):84-85.
[4]张宇,艾芳.栀子豉汤在精神科疾病中的应用[J].长春中医药大学学报,2010,26(2):207-208.
[5]乔利军,张新春,黄燕.《伤寒论》栀子汤类方方证探析[J].新中医,2017,49(6):160-162.
[6] REN Yanqing,ZHEN Yaqin,LI Baolin,et al.Study on detoxification of Sojae Semen Preparatum on liver toxicity induced by Gardeniae Fructus[J].Pharmacol&Clin Chin Materia Medica,2017,33(4):94-97.(in Chinese)任艳青,甄亚钦,李葆林,等.淡豆豉与栀子配伍降低栀子肝脏毒性的研究[J].中药药理与临床,2017,33(4):94-97.
[7] Jia W,Li HK,Zhao LP,et al.Gut microbiota:A potential new territory for drug targeting[J].Nat Rev Drug Discov,2008,7(2):123-129.
[8] CHEN Liyan,GUAN Xuelian,ZHANG Lei,et al.Regulatory effect of Sojae Semen Preparatum on 6common gut microbes[J].Chin J Microecol,2017,29(10):1122-1126.(in Chinese)陈丽艳,官雪莲,张蕾,等.淡豆豉对人体肠道六种常住菌的调节作用[J].中国微生态学杂志,2017,29(10):1122-1126.
[9] WANG Xiuhai.Preparation and pharmacodynamics studies on ZhiZiChiTangoral liquid[D].Harbin:Heilongjiang University of Chinese Medicine,2011.(in Chinese)王秀海.栀子豉汤口服液制备工艺及药效学研究[D].哈尔滨:黑龙江中医药大学,2011.
[10] ZHONG Xiaoyu,HUO Wuzhen,GONG Qiong,et al.Study status on compatibility of Chinese materia medica based on pharmacokinetics[J].Chin Trad Herbal Drugs,2013,44(21):3084-3088.(in Chinese)钟晓雨,霍务贞,龚琼,等.基于药动学的中药配伍研究现状[J].中草药,2013,44(21):3084-3088.
[11] MAI Lanyi,LI Yixuan,CHEN Yong,et al.Applications of mathematical statistics methods on compatibility researches of traditional Chinese medicines formulae[J].China J Chin Materia Medica,2014,29(10):1749-1755.(in Chinese)麦蓝尹,李怡萱,陈勇,等.基于数量统计方法学的中药复方配伍研究进展[J].中国中药杂志,2014,29(10):1749-1755.
[12] XIAO Hongbin,LIU Yanqiu,WANG Li,et al.Study on multi-component combination of Chinese prescriptions based on component interaction[J].World Sci Technol/Mod TCM&Materia Medica,2011,13(2):240-243.(in Chinese)肖红斌,刘艳秋,王莉,等.基于成分相互作用的中药复方组分配伍研究[J].世界科学技术·中医药现代化思路与方法,2011,13(2):240-243.
[13]杨化冰,邹小娟,孔明望,等.肠道微生态与传统中医思想内涵[J].中医杂志,2017,58(12):1070-1071.
[14]张季阶,于丽娴,徐灵芝,等.一株无毒脆弱拟杆菌的分离鉴定[J].宁夏医学杂志,1991,4(8):216-218.
[15]朱延旭,尚尔彬,王占红.BF-839脆弱拟杆菌对肉仔鸡生产性能的影响[J].现代畜牧兽医,2012(3):40-42.
[16] FENG Shuzhen,ZHANG Heiping.The research progress of Bacteroides fragilis[J].Microbiol China,2015,42(7):1366-1371.(in Chinese)冯淑贞,张和平.脆弱拟杆菌的研究进展[J].微生物学通报,2015,42(7):1366-1371.
[17] Salzman NH.The role of the microbiome in immune cell development[J]. Allergy Asthma Immunol,2014,113(6):293-298.
[18] Hsiao EY,McBride SW,Hsien S,et al.The microbiota modulates gut physiology and behavioral abnormalities associated with autism[J].Cell,2013,155(7):1451-1463.
[19] GAO Fang.Experimental study on treating the depression with decoction of capejasmine and fermented soybean[D].Fuzhou:FuJian University of Traditional Chinese Medicine,2007.(in Chinese)高芳.栀子豉汤治疗抑郁症的实验研究[D].福州:福建中医学院,2007.
[20]袁圣龙.栀子豉汤在心身疾病治疗中的应用[J].云南中医中药杂志,2013,34(6):76-77.
[21] Groen RN,de Clercq NC,Nieuwdorp M,et al.Gut microbiota,metabolism and psychopathology:A critical review and novel perspectives[J].Crit Rev Clin Lab Sci,2018,55(4):283-293.
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