淫羊藿苷对去卵巢骨质疏松大鼠骨细胞凋亡及骨组织OPG、RANKL mRNA表达影响

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英文篇名：Effect of Icariin on Apoptosis of Bone Cells and Expression of OPG,RANKL and mRNA in Bone Tissue of Ovariectomized Rats with Osteoporosis 作者：吴峻 英文作者：WU Jun;Baoji Vocational & Technical College; 关键词：淫羊藿苷 ; 骨质疏松 ; 大鼠 ; 骨细胞凋亡 ; OPG ; RANKL ; mRNA 英文关键词：icariin;;osteoporosis;;rats;;osteocyte apoptosis;;OPG;;RANKL mRNA 中文刊名：LZXB 英文刊名：Journal of Liaoning University of Traditional Chinese Medicine 机构：宝鸡职业技术学院; 出版日期：2019-01-16 16:34 出版单位：辽宁中医药大学学报 年：2019 期：v.21;No.178 基金：陕西省科技攻关计划项目(2015K09-16) 语种：中文; 页：LZXB201902005 页数：4 CN：02 ISSN：21-1543/R 分类号：21-24

摘要

目的:探讨淫羊藿苷对去卵巢骨质疏松大鼠骨细胞凋亡及骨组织骨保护素(OPG)、核因子κB受体活化因子配体(RANKL)mRNA表达的影响。方法:选取健康雌性SD大鼠70只,采用去卵巢法建立骨质疏松模型,造模成功的51只大鼠随机分为模型组、尼尔雌醇组、淫羊藿苷组,各17只;对照组15只接受假手术,各组给予相应的药物治疗。对大鼠股骨骨密度(BMD)、骨矿含量(BMC)、血清生化指标水平、骨细胞及成骨细胞凋亡指数(AI)、骨组织OPG mRNA、RANKL mRNA、核因子κβ受体活化因子(RANK)mRNA表达水平进行检测。结果:治疗后,与模型组比较,各组大鼠股骨BMD、BMC水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组大鼠血清骨钙素(BGP)、碱性磷酸酶(ALP)、钙(Ca)水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组大鼠骨细胞AI、成骨细胞AI较低,且淫羊藿苷组<尼尔雌醇组(P<0.05);各组大鼠骨组织OPG mRNA表达水平较高,且淫羊藿苷组>尼尔雌醇组(P<0.05);各组骨组织RANKL mRNA、RANK mRNA表达水平较低,且淫羊藿苷组<尼尔雌醇组(P<0.05)。结论:淫羊藿苷能增加去卵巢骨质疏松大鼠骨密度和骨矿含量,改善血清BGP、ALP、Ca水平,抑制骨细胞及成骨细胞凋亡,可能与上调骨组织OPG mRNA表达,下调RANKL mRNA、RANK mRNA表达有关。
        Objective:To analysis the effect of icariin on apoptosis of bone cells and expression of osteoprotegerin(OPG),nuclear factor kappa B receptor activator ligand(RANKL)mRNA in bone tissue of ovariectomized rats with osteoporosis. Methods:Seventy healthy female SD rats were selected,the osteoporosis model was established by ovariectomy,51 rats which successfully modeled were randomly divided into model group,nilestriol group and icariin group,17 rats for each group;15 rats in the control group underwent sham surgery,and all rats were given corresponding drug treatment. The femoral bone mineral density(BMD),bone mineral content(BMC),serum biochemical parameters,bone cell and osteoblast apoptosis index(AI),bone tissue OPG,mRNA,RANKL mRNA,nuclear factor kappa beta receptor activator(RANK)mRNA expression levels were detected. Results:After treatment,compared with model control group,the levels of BMD and BMC in the femur were higher in each group,and icariin group > nilestriol group(P<0.05);the serum osteocalcin(BGP),alkaline phosphatase(ALP),and calcium(Ca)levels were higher in rats of each group,and icariin group>nilestriol group(P<0.05);the bone cell AI and osteoblast AI were lower in each group,and icariin group nilestriol group(P<0.05);the expression of OPG mRNA in bone tissue of rats in each group were lower,and icariin group

引文

[1]游利.骨质疏松症的现状、筛查和预防[J].中国全科医学,2016,19(14):1616-1619.
    [2]笪巍伟,唐德志,舒冰,等.原发性骨质疏松症及椎体压缩性骨折的防治进展[J].广东医学,2016,37(11):1714-1717.
    [3]李春霖.合理应用骨代谢生化标志物,提高骨质疏松患者治疗的依从性[J].标记免疫分析与临床,2015,22(10):943-943.
    [4]朱彤彤,黄连弟,李俊玮,等.淫羊藿苷对去卵巢大鼠骨质疏松症的保护作用及其机制[J].吉林大学学报(医学版),2016,42(5):915-919.
    [5]刘海龙,卫荣.绝经后骨质疏松症中西医临床治疗进展[J].现代中西医结合杂志,2014,23(14):1594-1596.
    [6]刘宗权,宋敏,蒋宜伟,等.淫羊藿苷抗骨质疏松对去势大鼠PINP,NTx影响的实验研究[J].甘肃中医学院学报,2014,31(3):4-7.
    [7]祁珊珊,杨祎琦,庞田田,等.去卵巢法建立SD大鼠绝经后骨质疏松模型手术探讨[J].中国兽医杂志,2014,50(2):27-29.
    [8]Kaddah E,Elbadrawy A,Mahdi S,et al. Role of vascular endothelial growth factor expression in pathogenesis of postmenopausal osteoporosis[J]. Egyptian Rheumatology&Rehabilitation,2013,40(4):211.
    [9]孙伟明,刘爽.雌激素影响绝经后骨质疏松分子机制研究进展[J].中国老年学,2017,37(2):499-502.
    [10]高彦彬.中医治疗骨质疏松症[J].中国临床医生杂志,2013,41(6):66-68.
    [11]Jia C,Liu H,Li M,et al. Effects of icariin on cytokine-induced ankylosing spondylitis with fibroblastic osteogenesis and its molecular mechanism[J]. International Journal of Clinical&Experimental Pathology,2014,7(12):9104.
    [12]刘广飞,程才,王璐,等.淫羊藿苷治疗骨质疏松的研究进展[J].现代生物医学进展,2015,15(26):5185-5188.
    [13]Liu M,Zhong C,He R X,et al. Icariin associated with exercise therapy is an effective treatment for postmenopausal osteoporosis[J]. Chin Med J,2012,125(10):1784.
    [14]吴祖锋,袁垒,吴风晴,等.淫羊藿苷对骨质疏松症模型大鼠OPG/RANKL/RANK轴系统影响的实验研究[J].甘肃中医学院学报,2016,33(3):4-7.
    [15]魏双双,张治芬,黄哲人,等.雌激素对去卵巢大鼠骨组织Wnt16、β-catenin、OPG、RANKL表达的影响[J].浙江医学,2017,39(4):245-249.