温胆汤对肥胖痰湿证免疫及炎症细胞因子表达的影响
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摘要
目的:探讨祛痰名方温胆汤对高脂饮食诱导的肥胖SD大鼠免疫及炎症细胞因子表达的影响。方法:根据文献方法建立肥胖痰湿证大鼠模型后采用温胆汤灌胃进行干预,比较用药前后大鼠肥胖率、体质量、Lee’s指数、血脂(TG、TC、LDL-C、HDL-C)、免疫T淋巴细胞(CD~+_3、CD~+_4、CD~+_8)及炎症细胞因子(TNF-α、IL-6、IL-17和IL-22)表达的变化。结果:高脂饮食可成功建立肥胖大鼠模型,造模组大鼠肥胖率大于20%,体质量、Lee’s指数明显增高,有显著性差异(P<0.05);温胆汤干预可降低肥胖大鼠肥胖率、体质量和Lee’s指数(P<0.05),改善血脂水平(P<0.05),调整T淋巴细胞CD~+_3、CD~+_4、CD~+_8及CD~+_4/CD~+_8比值的表达,有效调节TNF-α、IL-6、IL-17和IL-22等相关炎症细胞因子的表达(P<0.05)。结论:温胆汤改善肥胖效果明显,其机理可能是通过调控机体免疫机制来改善肥胖炎症反应状态,进而达到纠正肥胖痰湿病理的作用,这为"治痰"名方温胆汤干预肥胖痰湿证提供了科学依据。
Objective: To investigate the effect of Wendan Decoction on the expression of immune and inflammatory cytokines in obese SD rats induced by high-fat diet.Methods: The obese rat model with syndrome of phlegm-dampness was set up according to the literature method and then gavage administration with Wendan Decoction to compare the obesity rate, body weight, Lee's index, blood lipids(TG, TC, LDL-C, HDL-C), immune T lymphocytes(CD~+_3, CD~+_4, CD~+_8) and inflammatory cytokines(TNF-α, IL-6, IL-17 and IL-22) before and after treatment.Results:High-fat diet may establish successfully obesity rat model, and the obesity rate of the model group was more than 20%.The body weight and Lee's index were significantly increased and there was significant difference compared with those of the normal group(P<0.05).The invervention of Wendan Decoction may reduce obesity rate, body weight and Lee's index(P<0.05), improve blood lipid level(P<0.05), regulate the expressions of CD~+_3, CD~+_4, CD~+_8 and CD~+_4/CD~+_8 ratio of T lymphocytes, and effectively regulate the expressions of TNF-α, IL-6, IL-17 and IL-22 related inflammatory cytokines(P<0.05).Conclusion: Wendan Decoction has obvious effect on improving obesity, and its mechanism may be through regulating the body's immune mechanism to improve the inflammatory state of obesity, and then to correct the pathological effect of obesity with phlegm dampness, which provides a scientific basis for the famous prescription Wendan Decoction for treating phlegm to interfere with obesity phlegm dampness syndrome and then get the role of correcting the phlegm dampness pathology of obesity and provides a scientific basis for Wendan Decoction intervene obesity with syndrome of phlegm-dampness.
Objective: To investigate the effect of Wendan Decoction on the expression of immune and inflammatory cytokines in obese SD rats induced by high-fat diet.Methods: The obese rat model with syndrome of phlegm-dampness was set up according to the literature method and then gavage administration with Wendan Decoction to compare the obesity rate, body weight, Lee's index, blood lipids(TG, TC, LDL-C, HDL-C), immune T lymphocytes(CD~+_3, CD~+_4, CD~+_8) and inflammatory cytokines(TNF-α, IL-6, IL-17 and IL-22) before and after treatment.Results:High-fat diet may establish successfully obesity rat model, and the obesity rate of the model group was more than 20%.The body weight and Lee's index were significantly increased and there was significant difference compared with those of the normal group(P<0.05).The invervention of Wendan Decoction may reduce obesity rate, body weight and Lee's index(P<0.05), improve blood lipid level(P<0.05), regulate the expressions of CD~+_3, CD~+_4, CD~+_8 and CD~+_4/CD~+_8 ratio of T lymphocytes, and effectively regulate the expressions of TNF-α, IL-6, IL-17 and IL-22 related inflammatory cytokines(P<0.05).Conclusion: Wendan Decoction has obvious effect on improving obesity, and its mechanism may be through regulating the body's immune mechanism to improve the inflammatory state of obesity, and then to correct the pathological effect of obesity with phlegm dampness, which provides a scientific basis for the famous prescription Wendan Decoction for treating phlegm to interfere with obesity phlegm dampness syndrome and then get the role of correcting the phlegm dampness pathology of obesity and provides a scientific basis for Wendan Decoction intervene obesity with syndrome of phlegm-dampness.
引文
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[7] 刘洪双,刘莉,隋艳波.加减黄连温胆汤治疗代谢综合征(痰湿蕴结证)的临床研究[J].中国中医急症,2016,25(10):1837-1839,1850.
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[10] 马祎喆,周红文.免疫细胞在脂肪组织炎症中的调控作用[J].南京医科大学学报(自然科学版),2016,36(1):19-25.
[11] 刘涛,徐秋玲,杨叔禹.高脂饮食诱导痰湿证动物模型的建立与评价[J].长春中医药大学学报,2009,25(3):333-335.
[12] 杨海燕,王萍,喻松仁.温胆汤对大鼠肥胖评价指标及血糖血脂水平的影响[J].中华中医药学刊,2017,35(3):544-546.
[13] 宋剑南,刘东远,牛晓红.高脂血症与中医痰浊关系的实验研究[J].中国中医基础医学杂志,1995,1(1):49.
[14] 杨海燕,王萍.温胆汤减轻肥胖大鼠体重及对血清脂联素水平的影响[J].江西中医药,2014,35(3):14-16.
[15] Chandler PC,Viana JB,Oswald KD,et al.Feedingresponse to melanocortin agonist predicts preference for and obesity from a High-fat diet[J].Physiol Behav,2005,85(2):221-230.
[16] 李雨庭,范琳琳,袁茵,等.黄连温胆汤药理作用及临床应用研究进展[J].中医药学报,2018,46(2):115-119.
[17] 邱晓堂.张永杰应用温胆汤经验[J].河南中医,2015,35(3):505-506.
[18] 喻松仁,舒晴,王萍,等.肥胖痰湿证形成机制及证治规律探微[J].辽宁中医杂志,2017,44(12):2547-2548.
[19] 杨海燕,王萍.温胆汤干预肥胖及相关疾病的表观遗传学机制探讨[J].中医研究,2014,27(11):5-7.
[20] 喻松仁,王萍,舒晴,等.肥胖痰湿衍变规律探析[J].中华中医药杂志,2018,33(4):1483-1485.
[21] 万生芳.红芪多糖对脾虚大鼠T淋巴细胞亚群的影响[J].中医研究,2012,25(11):73-75.
[22] Deiuliis J,Shah Z,Shah N,et al.Visceral a dipose inflammation in obesity is Associated with critical alterations in tregulatory cell numbers[J].PLoS One, 2011,6(1):163-173.
[23] 董莉君,王辉.T细胞亚群在肥胖引起炎症中的作用[J].细胞与分子免疫学杂志,2015,31(9):1292-1295.
[24] 刘砺,张丽娜,满富丽,等.肥胖与免疫研究进展[J].北京医学,2017,39(6):631-633.
[25] 吕兰秋,傅君芬.儿童肥胖与细胞免疫功能[J].中国儿童保健杂志,2005,13(6):525-526.
[26] 司原成,王晓芳,汪弋力,等.营养性肥胖之免疫机制研究进展[J].中国实验方剂学杂志,2016,22(6):230-234.
[2] 王增武,郝光,王馨,等.我国中年人群超重/肥胖现况及心血管病危险因素聚集分析[J].中华流行病学杂志,2014,35(4):354-358.
[3] 曹平良,邱元芝,管让宪,等.江西省高级公务员代谢综合征的调查分析[J].实用临床医学(江西),2009,10(4):100-102.
[4] 舒晴,喻松仁,王萍.从中医体质角度探讨肥胖的形成机制[J].江西中医药,2017,48(2):8-9,15.
[5] 史晓娜,陶枫,陆灏,等.肥胖证候和证素分布特征的文献研究[J].上海中医药大学学报,2014,28(4):53-57.
[6] 连东辉.加味温胆汤治疗痰湿壅盛型肥胖性高血压病 60例[J].河南中医,2012,32(7):913-914.
[7] 刘洪双,刘莉,隋艳波.加减黄连温胆汤治疗代谢综合征(痰湿蕴结证)的临床研究[J].中国中医急症,2016,25(10):1837-1839,1850.
[8] Hotamisligil GS.Inflammatory pathways and insulin action [J].Int J Obes Relat Metab Disord,2003,27(Suppl 3):53-55.
[9] 郑璐玉,杨玲玲,李玲孺,等.液相芯片技术检测痰湿体质人群TNF-α、IL-6、CRP及MCP-1的表达研究[J].中国中西医结合杂志,2013,33(7):920-923.
[10] 马祎喆,周红文.免疫细胞在脂肪组织炎症中的调控作用[J].南京医科大学学报(自然科学版),2016,36(1):19-25.
[11] 刘涛,徐秋玲,杨叔禹.高脂饮食诱导痰湿证动物模型的建立与评价[J].长春中医药大学学报,2009,25(3):333-335.
[12] 杨海燕,王萍,喻松仁.温胆汤对大鼠肥胖评价指标及血糖血脂水平的影响[J].中华中医药学刊,2017,35(3):544-546.
[13] 宋剑南,刘东远,牛晓红.高脂血症与中医痰浊关系的实验研究[J].中国中医基础医学杂志,1995,1(1):49.
[14] 杨海燕,王萍.温胆汤减轻肥胖大鼠体重及对血清脂联素水平的影响[J].江西中医药,2014,35(3):14-16.
[15] Chandler PC,Viana JB,Oswald KD,et al.Feedingresponse to melanocortin agonist predicts preference for and obesity from a High-fat diet[J].Physiol Behav,2005,85(2):221-230.
[16] 李雨庭,范琳琳,袁茵,等.黄连温胆汤药理作用及临床应用研究进展[J].中医药学报,2018,46(2):115-119.
[17] 邱晓堂.张永杰应用温胆汤经验[J].河南中医,2015,35(3):505-506.
[18] 喻松仁,舒晴,王萍,等.肥胖痰湿证形成机制及证治规律探微[J].辽宁中医杂志,2017,44(12):2547-2548.
[19] 杨海燕,王萍.温胆汤干预肥胖及相关疾病的表观遗传学机制探讨[J].中医研究,2014,27(11):5-7.
[20] 喻松仁,王萍,舒晴,等.肥胖痰湿衍变规律探析[J].中华中医药杂志,2018,33(4):1483-1485.
[21] 万生芳.红芪多糖对脾虚大鼠T淋巴细胞亚群的影响[J].中医研究,2012,25(11):73-75.
[22] Deiuliis J,Shah Z,Shah N,et al.Visceral a dipose inflammation in obesity is Associated with critical alterations in tregulatory cell numbers[J].PLoS One, 2011,6(1):163-173.
[23] 董莉君,王辉.T细胞亚群在肥胖引起炎症中的作用[J].细胞与分子免疫学杂志,2015,31(9):1292-1295.
[24] 刘砺,张丽娜,满富丽,等.肥胖与免疫研究进展[J].北京医学,2017,39(6):631-633.
[25] 吕兰秋,傅君芬.儿童肥胖与细胞免疫功能[J].中国儿童保健杂志,2005,13(6):525-526.
[26] 司原成,王晓芳,汪弋力,等.营养性肥胖之免疫机制研究进展[J].中国实验方剂学杂志,2016,22(6):230-234.
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