Box-Behnken设计-响应面法优化木鳖子霜炮制工艺
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摘要
目的采用Box-Behnken设计-响应面法(BBD-RSM)优选木鳖子霜的最佳炮制工艺。方法以丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯、β-谷甾醇、齐墩果酸含量及脂肪油含量的指标归一值(OD)为综合评价指标,采用3因素3水平的BBD-RSM设计试验,考察烘制时间、烘制温度、压制时间对木鳖子霜炮制工艺的影响。结果优化得最佳工艺条件:烘制时间1.68 h、烘制温度80℃、压制时间30 min,OD值为0.777。考虑实际情况,对烘制时间进行微调得出木鳖子最佳炮制工艺,即烘制时间为1.7 h、烘制温度为80℃、压制时间为30 min。并通过所得条件平行制备3批样品,计算得OD值分别为0.779、0.783、0.766,其RSD为1.15%。结论采用BBD-RSM优选的炮制工艺稳定,模型预测效果良好,建立了木鳖子霜炮制新工艺。
Objective To optimize the optimum processing technology of Momordicae Semen cream using Box-Behnken design-response surface method(BBD-RSM).Methods The overall desirability(OD) of indexes of the content of 3-O-β-Dglucuronic acid methyl ester,β-sitosterol,and oleanolic acid and the content of fatty oil in gypsogenin was comprehensively evaluated,and BBD-RSM with three factors and three levels was used to study the effect of baking temperature and time,and pressing time on the processing technology of Momordicae Semen cream.Results According to the experiment,the optimum processing conditions were optimized:the baking time 1.68 h,the baking temperature 80 ℃,the pressing time 30 min,and the OD value was 0.777.Considering the actual situation,the optimum processing technology of Momordicae Semen was obtained by fine-tuning the baking time(A).That was,the baking time was 1.7 h,the baking temperature was 80 ℃,and the pressing time was 30 min.Also,the calculated OD values were 0.779,0.783,0.766,and its RSD was 1.15% through the obtained conditions in parallel with three batches of samples.Conclusion The processing technology optimized by Box-Behnken design-response surface method has the advantages of stable,good model prediction effect and a new processing technology for Momordicae Semen cream production.
Objective To optimize the optimum processing technology of Momordicae Semen cream using Box-Behnken design-response surface method(BBD-RSM).Methods The overall desirability(OD) of indexes of the content of 3-O-β-Dglucuronic acid methyl ester,β-sitosterol,and oleanolic acid and the content of fatty oil in gypsogenin was comprehensively evaluated,and BBD-RSM with three factors and three levels was used to study the effect of baking temperature and time,and pressing time on the processing technology of Momordicae Semen cream.Results According to the experiment,the optimum processing conditions were optimized:the baking time 1.68 h,the baking temperature 80 ℃,the pressing time 30 min,and the OD value was 0.777.Considering the actual situation,the optimum processing technology of Momordicae Semen was obtained by fine-tuning the baking time(A).That was,the baking time was 1.7 h,the baking temperature was 80 ℃,and the pressing time was 30 min.Also,the calculated OD values were 0.779,0.783,0.766,and its RSD was 1.15% through the obtained conditions in parallel with three batches of samples.Conclusion The processing technology optimized by Box-Behnken design-response surface method has the advantages of stable,good model prediction effect and a new processing technology for Momordicae Semen cream production.
引文
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[7]Beringhs A O,Dalmina M,Creczynski-Pasa T B,et al.Response surface methodology IV-optimal design applied to the performance improvement of an RP-HPLC-UVmethod for the quantification of phenolic acids in Cecropia glaziovii products[J].Revista Brasileira Farmacognosia,2015,25(5):513-521.
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[9]李存玉,马赟,龚柔佳,等.响应面分析法耦合调节Donnan效应优化苦参提取液的纳滤浓缩工艺[J].中草药,2016,47(19):3395-3400.
[10]余小翠,刘高峰.响应面分析法在中药提取和制备工艺中的应用[J].中药材,2010,33(10):1651-1655.
[11]樊根遥,孙收杰.响应面法在优化中药提取工艺中的应用[J].应用科技,2015(6):139.
[12]陈洁,戴衍朋,孙立立,等.Box-Behnken设计-效应面法优选甘草切制工艺[J].中草药,2013,44(12):1579-1583.
[13]李化,柯华香,李发洁,等.Box-Behnken响应面法优选五味子多糖双水相提取工艺[J].中药材,2016,39(3):593-597.
[14]张丹,蒋海强,张久严.木鳖子中脂肪油的提取及GC-MS联用分析[J].中成药,2010,32(2):314-315.
[15]王晖,陈丽,陈垦,等.多指标综合评价方法及权重系数的选择[J].广东药学院学报,2007,23(5):583-589.
[16]刘飞燕,张建方.多指标回归综合评分[J].数理统计与管理,2014,33(3):408-415.
[17]王协和,夏林丽,呼梅,等.Box-Behnken响应曲面结合多指标综合评分法优选通脉复方提取工艺[J].中国医院药学杂志,2017,37(8):712-716.
[2]耿艺曼,赵连梅,朱秀丽,等.木鳖子对羟基桂皮醛对黑素瘤B16细胞分化的影响及其机制[J].中草药,2013,44(14):1951-1956.
[3]Jung K,Lee D,Yu J S,et al.Protective effect and mechanism of action of saponins isolated from the seeds of gac(Momordica cochinchinensis Spreng.)against cisplatin-induced damage in LLC-PK1 kidney cells[J].Bioorg Med Chem Lett,2016,26(5):1466-1470.
[4]赵连梅,耿艺曼,孙士萍,等.木鳖子单体化合物对羟基桂皮醛诱导小鼠黑素瘤B16细胞的分化及其机制[J].中国肿瘤生物治疗杂志,2014,21(3):282-287.
[5]郑蕾,何昊,方怡,等.木鳖子抗肿瘤有效作用部位筛选及作用机制探讨[J].中国实验方剂学杂志,2017,23(9):152-157.
[6]于向艳,崔雯萱,孙士萍,等.木鳖子对羟基桂皮醛对小鼠黑素移植瘤生长的抑制作用及机制研究[J].中草药,2016,47(10):1740-1745.
[7]Beringhs A O,Dalmina M,Creczynski-Pasa T B,et al.Response surface methodology IV-optimal design applied to the performance improvement of an RP-HPLC-UVmethod for the quantification of phenolic acids in Cecropia glaziovii products[J].Revista Brasileira Farmacognosia,2015,25(5):513-521.
[8]K?rbahti B K,Demirbüken P.Electrochemical oxidation of resorcinol in aqueous medium using boron-doped diamond anode:Reaction kinetics and process optimization with response surface methodology[J].Front Chem,2017,5:75.
[9]李存玉,马赟,龚柔佳,等.响应面分析法耦合调节Donnan效应优化苦参提取液的纳滤浓缩工艺[J].中草药,2016,47(19):3395-3400.
[10]余小翠,刘高峰.响应面分析法在中药提取和制备工艺中的应用[J].中药材,2010,33(10):1651-1655.
[11]樊根遥,孙收杰.响应面法在优化中药提取工艺中的应用[J].应用科技,2015(6):139.
[12]陈洁,戴衍朋,孙立立,等.Box-Behnken设计-效应面法优选甘草切制工艺[J].中草药,2013,44(12):1579-1583.
[13]李化,柯华香,李发洁,等.Box-Behnken响应面法优选五味子多糖双水相提取工艺[J].中药材,2016,39(3):593-597.
[14]张丹,蒋海强,张久严.木鳖子中脂肪油的提取及GC-MS联用分析[J].中成药,2010,32(2):314-315.
[15]王晖,陈丽,陈垦,等.多指标综合评价方法及权重系数的选择[J].广东药学院学报,2007,23(5):583-589.
[16]刘飞燕,张建方.多指标回归综合评分[J].数理统计与管理,2014,33(3):408-415.
[17]王协和,夏林丽,呼梅,等.Box-Behnken响应曲面结合多指标综合评分法优选通脉复方提取工艺[J].中国医院药学杂志,2017,37(8):712-716.
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