不同李资源光合效能及其季节变化比较与聚类分析
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摘要
[目的]本文旨在研究不同李资源在生长季内叶片光合能力的差异,为李的高光效育种提供理论依据。[方法]采用Li-6400便携式光合测定系统分别于2017年春季(5月下旬)、夏季(8月上旬)和秋季(10月上旬)对不同种源李资源的22个品种进行光合日变化测定。对净光合速率(P_n)日积分值、蒸腾速率(T_r)日积分值、气孔导度(G_s)和水分利用效率(WUE)进行数值分类,并进行主成分分析、聚类分析和判定分析。[结果]李资源的P_n日积分均值春季最高,春、夏、秋呈现逐渐降低趋势; WUE日均值夏季最低,春、秋季节较高。将不同李资源按P_n高低可划分为高、中、低光合速率3类;按WUE高低可划分为高、较高、中、低水分利用效率4类。进一步主成分分析确定P_n日积分值、T_r日积分值、G_s和WUE为影响李光合生理的主导因子。对各指标进行系统聚类分析,最终不同李资源被聚为3类,并筛选出6个综合光合性状较优的品种:黑刺李‘Damas~(-1)869’,欧洲李‘大玫瑰’,中国李‘芙蓉李’‘嘉庆子’,杂交李‘安哥诺’‘圣玫瑰’。[结论]在3个季节中,不同李资源的光合能力存在差异,通过聚类分析的方法可以筛选出适用于高光效育种的李资源。
[Objectives]The research aimed to study the difference of leaf photosynthetic capacity of different plum resources in the growing season and provide the theoretical basis for the high light efficiency breeding of plum. [Methods]The photosynthesis daily changes of 22 varieties from different provenances of plum germplasm resources were measured using Li-6400 portable photosynthesis system respectively in spring( late May),summer( early August) and autumn( early October),2017. Classified the diuenal integral value( DIV) of net photosynthetic rate( P_n),DIV of T_r,G_sand water use efficiency( WUE),and the principal component,the clustering and the decision analysis were carried out. [Results]Plum resources had the highest P_nin spring and it showed a trend of gradually decreasing in three seasons. Daily mean value of WUE was the lowest in summer and higher in spring and autumn.Different plum resources were classified into 3 categories: high,relatively high,middle and low photosynthetic rate according to P_n.According to the values of WUE,four categories of high,medium,and low water use efficiency were divided. Further principal component analysis showed that DIV of P_n,DIV of T_r,G_sand WUE were the main factors influencing photosynthetic physiology.According to the four indicators,different plum resources were clustered into three categories. Six selected varieties with better comprehensive photosynthetic characters: Prunus spisona'Damas~(-1)869',Prunus domestica'Great Rosa',Prunus salicina'Furongli'and'Jiaqingzi ',hybrid plum'Angeleno 'and'Sata Rosa ',were used for high photosynthetic efficiency breeding of plum.[Conclusions]The photosynthetic capacity of different plum resources are differences in three seasons. Through the clustering analysis method the plum resource can be selected which is suitable for the high light efficiency breeding.
[Objectives]The research aimed to study the difference of leaf photosynthetic capacity of different plum resources in the growing season and provide the theoretical basis for the high light efficiency breeding of plum. [Methods]The photosynthesis daily changes of 22 varieties from different provenances of plum germplasm resources were measured using Li-6400 portable photosynthesis system respectively in spring( late May),summer( early August) and autumn( early October),2017. Classified the diuenal integral value( DIV) of net photosynthetic rate( P_n),DIV of T_r,G_sand water use efficiency( WUE),and the principal component,the clustering and the decision analysis were carried out. [Results]Plum resources had the highest P_nin spring and it showed a trend of gradually decreasing in three seasons. Daily mean value of WUE was the lowest in summer and higher in spring and autumn.Different plum resources were classified into 3 categories: high,relatively high,middle and low photosynthetic rate according to P_n.According to the values of WUE,four categories of high,medium,and low water use efficiency were divided. Further principal component analysis showed that DIV of P_n,DIV of T_r,G_sand WUE were the main factors influencing photosynthetic physiology.According to the four indicators,different plum resources were clustered into three categories. Six selected varieties with better comprehensive photosynthetic characters: Prunus spisona'Damas~(-1)869',Prunus domestica'Great Rosa',Prunus salicina'Furongli'and'Jiaqingzi ',hybrid plum'Angeleno 'and'Sata Rosa ',were used for high photosynthetic efficiency breeding of plum.[Conclusions]The photosynthetic capacity of different plum resources are differences in three seasons. Through the clustering analysis method the plum resource can be selected which is suitable for the high light efficiency breeding.
引文
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[5] Venema J H,Linger P,Heusden A W,et al. The inheritance of chilling tolerance in tomato(Lycopersicon spp.)[J]. Plant Biology,2005,7(2):118-130.
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[7]张宗琼,张晓丽,王强,等.东南亚水稻种质资源的光合特性比较及聚类分析[J].南方农业学报,2014,45(8):1358-1362.Zhang Z Q,Zhang X L,Wang Q,et al. Comparison and cluster analysis of photosynthetic characters of different rice germplasm resources in Southeast Asia[J]. Journal of Southern Agriculture,2014,45(8):1358-1362(in Chinese with English abstract).
[8]张贵合,郭华春.马铃薯不同品种(系)的光合特性比较与聚类分析[J].作物学报,2017,43(7):1067-1076.Zhang G H,Guo H C. Comparison of photosynthetic characteristics and cluster analysis in potato varieties(lines)[J]. Acta Agronomica Sinica,2017,43(7):1067-1076(in Chinese with English abstract).
[9]张加延.我国李杏种质资源调查研究的突破性进展[J].园艺与种苗,2011(2):7-10,37.Zhang J Y. Disruptive progress of plum and apricot germplasm resources investigation in China[J]. Horticulture and Seed,2011(2):7-10,37(in Chinese with English abstract).
[10]王玉柱,杨丽,阎爱玲,等.李品种选育研究进展[J].果树学报,2002,19(5):340-345.Wang Y Z,Yang L,Yan A L,et al. Progress of plum breeding in the world[J]. Journal of Fruit Science,2002,19(5):340-345(in Chinese with English abstract).
[11]于泽源,许姣卉,霍俊伟.李光合特性的研究[J].东北农业大学学报,2004,35(3):315-317.Yu Z Y,Xu J H,Huo J W. Studies on photosynthetic characteristics of plum[J]. Journal of Northeast Agricultural University,2004,35(3):315-317(in Chinese with English abstract).
[12]张吉祥.黑琥珀李光合特性的研究[J].果树学报,2005,22(1):84-86.Zhang J X. Studies on photosynthetic characteristics of black amber plum cultivar[J]. Journal of Fruit Science,2005,22(1):84-86(in Chinese with English abstract).
[13]尹利德.三个李品种幼树光合特性的研究[D].武汉:华中农业大学,2004.Yin L D. The research on the photosynthetic characteristics of three plum varieties[D]. Wuhan:Huazhong Agricultural University,2004(in Chinese with English abstract).
[14]黄诗扬,卢宝明,任建武,等.太阳李不同叶位光合速率及叶绿素荧光参数特征[J].福建林业科技,2016,43(2):77-79,116.Huang S Y,Lu B M,Ran J W,et al. Measurement of chlorophyll fluorescence parameters and photosynthesis of different blade position in Prunus ceraifera‘Pissardii’[J]. Journal of Fujian Forestry Science and Technology,2016,43(2):77-79,116(in Chinese with English abstract).
[15]刘元铅,王开芳,杜华兵,等. 11个李品种光合速率及蒸腾强度测试研究[J].山东林业科技,2004(1):15-16.Liu Y Q,Wang K F,Du H B,et al. Studies on photosynthetic rate and transpiration rate of 11 plum cultivars[J]. Journal of Shandong Forestry Science and Technology,2004(1):15-16(in Chinese).
[16] Li W W,Wei Y J,Feng B B,et al. Comparison of photosynthetic characteristics among different Pruns domestica×armeniaca cultivars[J].Agricultural Science and Technology,2016,17(3):485-489.
[17]巴图巴雅尔,徐业勇,王明,等. 4个引种杏李品种气体交换特征及其对环境因子的响应[J].江苏农业科学,2014,42(9):123-125.Batu Bayaer,Xu Y Y,Wang M,et al. Gas exchange characteristics and its response to environmental factors of 4 apricot plum cultivars[J].Jiangsu Agrricultural Sciences,2014,42(9):123-125(in Chinese).
[18]王明,许业勇,杨红丽.三个引进欧洲李品种光合特性对比研究[J].南方农业,2015,9(18):10-11,13.Wang M,Xu Y Y,Yang H L. Comparison of photosynthetic characteristics of 3 European plum[J]. South China Agriculture,2015,9(18):10-11,13(in Chinese).
[19]庄猛,姜卫兵,花国平,等.金边黄杨与大叶黄杨光合特性的比较[J].植物生理学通讯,2006,42(1):39-42.Zhuang M,Jiang W B,Hua G P,et al. Comparison of the photosynthetic characteristics of Euonymus japonicus L.f. aureo-marginatus Rehd. and Euonymus japonicus L.[J]. Plant Physiology Communications,2006,42(1):39-42(in Chinese with English abstract).
[20]曹珂,朱更瑞,冯义彬,等.杏、李和杏李光合特性比较及优异种质筛选[J].植物遗传资源学报,2007,8(3):331-335.Cao K,Zhu G R,Feng Y B,et al. Comparison of photosynthetic characters in different apricot,plum and apricot plum and the screening of outstanding germplasm[J]. Journal of Plant Genetic Resources,2007,8(3):331-335(in Chinese with English abstract).
[21]张加延,周恩.中国果树志·李卷[M].北京:中国林业出版社,1998.Zhang J Y,Zhou E. Chinese Fruit Tree of Plum[M]. Beijing:China Frestry Publishing Houes,1998(in Chinese).
[22]张斌斌,姜卫兵,韩键,等.桃光合性能杂种优势的秋季节日变化特征[J].中国生态农业学报,2011,19(1):82-86.Zhang B B,Jiang W B,Han J,et al. Diurnal variation characteristics of heterosis of peach photosynthetic performance in autumn[J]. Chinese Journal of Eco-Agriculture,2011,19(1):82-86(in Chinese with English abstract).
[23]罗俊,周会,张木清,等.能源甘蔗主要经济和光合性状的遗传分析[J].应用与环境生物学报,2004,10(3):268-273.Luo J,Zhou H,Zhang M Q,et al. Genetic analysis of main economic and photosynthetic traits in energy sugarcane[J]. Chin J Appl Environ Biol,2004,10(3):268-273(in Chinese with English abstract).
[24]乔玉山.中国李RAPD、ISSR和SSR反应体系的建立及其品种资源遗传多样性分析[D].南京:南京农业大学,2003.Qiao Y S. Establishment of RAPD,ISSR,and SSR reaction system and analysis of genetic diversity of Japanese plum cultivars[D]. Nanjing:Nanjing Agricultual University,2003(in Chinese with English abstract).
[25]曹生奎,冯起,司建华,等.植物叶片水分利用效率研究综述[J].生态学报,2009,29(7):3882-3892.Cao S K,Feng Q,Si J H,et al. Summary on the plantwater use efficiency at leaf level[J]. Acta Ecilogica Sinica,2009,29(7):3882-3892(in Chinese with English abstract).
[2] Proietti P,Famiana F. Diurnal and seasonal changes in photosynthetic characteristics in different olive(Olea europaea L.)cultivars[J].Photosynthetica,2002,40(2):171-176.
[3]姜卫兵,庄猛,沈志军,等.不同季节红叶桃、紫叶李的光合特性研究[J].园艺学报,2006,33(3):577-582.Jing W B,Zhuang M,Sheng Z J,et al. Study on the photosynthetic characteristics of red-leaf peach and purple-leaf plum in different seasons[J].Acta Horticulturae Sinica,2006,33(3):577-582(in Chinese with English abstract).
[4]张斌斌,姜卫兵,韩键,等.桃光合性状杂种优势研究[J].园艺学报,2011,38(1):25-34.Zhang B B,Jiang W B,Han J,et al. Research on heterosis of photosynthetic characteristcs of peach[J]. Acta Horticulturae Sinica,2011,38(1):25-34(in Chinese with English abstract).
[5] Venema J H,Linger P,Heusden A W,et al. The inheritance of chilling tolerance in tomato(Lycopersicon spp.)[J]. Plant Biology,2005,7(2):118-130.
[6]郭春芳,孙云,陈常颂,等.茶树品种光合与水分利用特性比较及聚类分析[J].作物学报,2008,34(10):1797-1804.Guo C F,Sun Y,Chen C S,et al. Comparison and cluster analysis of photosynthetic characters and water use efficiency in tea(Camellia sinensis)cultivars[J]. Acta Agronomica Sinica,2008,34(10):1797-1804(in Chinese with English abstract).
[7]张宗琼,张晓丽,王强,等.东南亚水稻种质资源的光合特性比较及聚类分析[J].南方农业学报,2014,45(8):1358-1362.Zhang Z Q,Zhang X L,Wang Q,et al. Comparison and cluster analysis of photosynthetic characters of different rice germplasm resources in Southeast Asia[J]. Journal of Southern Agriculture,2014,45(8):1358-1362(in Chinese with English abstract).
[8]张贵合,郭华春.马铃薯不同品种(系)的光合特性比较与聚类分析[J].作物学报,2017,43(7):1067-1076.Zhang G H,Guo H C. Comparison of photosynthetic characteristics and cluster analysis in potato varieties(lines)[J]. Acta Agronomica Sinica,2017,43(7):1067-1076(in Chinese with English abstract).
[9]张加延.我国李杏种质资源调查研究的突破性进展[J].园艺与种苗,2011(2):7-10,37.Zhang J Y. Disruptive progress of plum and apricot germplasm resources investigation in China[J]. Horticulture and Seed,2011(2):7-10,37(in Chinese with English abstract).
[10]王玉柱,杨丽,阎爱玲,等.李品种选育研究进展[J].果树学报,2002,19(5):340-345.Wang Y Z,Yang L,Yan A L,et al. Progress of plum breeding in the world[J]. Journal of Fruit Science,2002,19(5):340-345(in Chinese with English abstract).
[11]于泽源,许姣卉,霍俊伟.李光合特性的研究[J].东北农业大学学报,2004,35(3):315-317.Yu Z Y,Xu J H,Huo J W. Studies on photosynthetic characteristics of plum[J]. Journal of Northeast Agricultural University,2004,35(3):315-317(in Chinese with English abstract).
[12]张吉祥.黑琥珀李光合特性的研究[J].果树学报,2005,22(1):84-86.Zhang J X. Studies on photosynthetic characteristics of black amber plum cultivar[J]. Journal of Fruit Science,2005,22(1):84-86(in Chinese with English abstract).
[13]尹利德.三个李品种幼树光合特性的研究[D].武汉:华中农业大学,2004.Yin L D. The research on the photosynthetic characteristics of three plum varieties[D]. Wuhan:Huazhong Agricultural University,2004(in Chinese with English abstract).
[14]黄诗扬,卢宝明,任建武,等.太阳李不同叶位光合速率及叶绿素荧光参数特征[J].福建林业科技,2016,43(2):77-79,116.Huang S Y,Lu B M,Ran J W,et al. Measurement of chlorophyll fluorescence parameters and photosynthesis of different blade position in Prunus ceraifera‘Pissardii’[J]. Journal of Fujian Forestry Science and Technology,2016,43(2):77-79,116(in Chinese with English abstract).
[15]刘元铅,王开芳,杜华兵,等. 11个李品种光合速率及蒸腾强度测试研究[J].山东林业科技,2004(1):15-16.Liu Y Q,Wang K F,Du H B,et al. Studies on photosynthetic rate and transpiration rate of 11 plum cultivars[J]. Journal of Shandong Forestry Science and Technology,2004(1):15-16(in Chinese).
[16] Li W W,Wei Y J,Feng B B,et al. Comparison of photosynthetic characteristics among different Pruns domestica×armeniaca cultivars[J].Agricultural Science and Technology,2016,17(3):485-489.
[17]巴图巴雅尔,徐业勇,王明,等. 4个引种杏李品种气体交换特征及其对环境因子的响应[J].江苏农业科学,2014,42(9):123-125.Batu Bayaer,Xu Y Y,Wang M,et al. Gas exchange characteristics and its response to environmental factors of 4 apricot plum cultivars[J].Jiangsu Agrricultural Sciences,2014,42(9):123-125(in Chinese).
[18]王明,许业勇,杨红丽.三个引进欧洲李品种光合特性对比研究[J].南方农业,2015,9(18):10-11,13.Wang M,Xu Y Y,Yang H L. Comparison of photosynthetic characteristics of 3 European plum[J]. South China Agriculture,2015,9(18):10-11,13(in Chinese).
[19]庄猛,姜卫兵,花国平,等.金边黄杨与大叶黄杨光合特性的比较[J].植物生理学通讯,2006,42(1):39-42.Zhuang M,Jiang W B,Hua G P,et al. Comparison of the photosynthetic characteristics of Euonymus japonicus L.f. aureo-marginatus Rehd. and Euonymus japonicus L.[J]. Plant Physiology Communications,2006,42(1):39-42(in Chinese with English abstract).
[20]曹珂,朱更瑞,冯义彬,等.杏、李和杏李光合特性比较及优异种质筛选[J].植物遗传资源学报,2007,8(3):331-335.Cao K,Zhu G R,Feng Y B,et al. Comparison of photosynthetic characters in different apricot,plum and apricot plum and the screening of outstanding germplasm[J]. Journal of Plant Genetic Resources,2007,8(3):331-335(in Chinese with English abstract).
[21]张加延,周恩.中国果树志·李卷[M].北京:中国林业出版社,1998.Zhang J Y,Zhou E. Chinese Fruit Tree of Plum[M]. Beijing:China Frestry Publishing Houes,1998(in Chinese).
[22]张斌斌,姜卫兵,韩键,等.桃光合性能杂种优势的秋季节日变化特征[J].中国生态农业学报,2011,19(1):82-86.Zhang B B,Jiang W B,Han J,et al. Diurnal variation characteristics of heterosis of peach photosynthetic performance in autumn[J]. Chinese Journal of Eco-Agriculture,2011,19(1):82-86(in Chinese with English abstract).
[23]罗俊,周会,张木清,等.能源甘蔗主要经济和光合性状的遗传分析[J].应用与环境生物学报,2004,10(3):268-273.Luo J,Zhou H,Zhang M Q,et al. Genetic analysis of main economic and photosynthetic traits in energy sugarcane[J]. Chin J Appl Environ Biol,2004,10(3):268-273(in Chinese with English abstract).
[24]乔玉山.中国李RAPD、ISSR和SSR反应体系的建立及其品种资源遗传多样性分析[D].南京:南京农业大学,2003.Qiao Y S. Establishment of RAPD,ISSR,and SSR reaction system and analysis of genetic diversity of Japanese plum cultivars[D]. Nanjing:Nanjing Agricultual University,2003(in Chinese with English abstract).
[25]曹生奎,冯起,司建华,等.植物叶片水分利用效率研究综述[J].生态学报,2009,29(7):3882-3892.Cao S K,Feng Q,Si J H,et al. Summary on the plantwater use efficiency at leaf level[J]. Acta Ecilogica Sinica,2009,29(7):3882-3892(in Chinese with English abstract).
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