菲牛蛭与蛋白饲料原料氨基酸平衡的灰色关联度分析
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摘要
【目的】研究菲牛蛭蛭体氨基酸与蛋白饲料原料氨基酸平衡,为菲牛蛭氨基酸平衡饲料开发提供参考。【方法】采用高效液相柱前衍生方法测定菲牛蛭蛭体和11种蛋白饲料的氨基酸含量,通过灰色关联分析方法中的灰色关联度评价11种蛋白饲料与菲牛蛭蛭体氨基酸平衡程度。【结果】11种蛋白饲料与菲牛蛭蛭体氨基酸平衡的灰色关联度从高到低为牛肉粉>大豆浓缩蛋白>血浆蛋白粉>(猪肉粉=鸡肉粉)>(鱼粉>大豆分离蛋白)>血粉>血球蛋白粉>豆粕>全脂奶粉。【结论】牛肉粉、大豆浓缩蛋白、血浆蛋白粉、猪肉粉、鸡肉粉、鱼粉和大豆分离蛋白7种蛋白饲料原料与菲牛蛭蛭体氨基酸灰色关联度均在0.8以上,可优选作为开发菲牛蛭人工饲料的蛋白饲料原料。
[Purpose]We aimed to study the amino acid balance of the Hirudinaria manillensis and protein feed raw material, in order to provide reference for development of amino acid balanced feed for the leech of the H. manillensis.[Method]The high performance liquid column derivative method was used to determine the content of amino acid in the H. manillensis and 11 protein feedstuffs, in order to evaluate gray correlation degree of amino acids balance between 11 kinds of protein feed raw materials and H. manillensis.[Result]The grey correlation degree of amino acids balance between11 kinds of protein feed raw material and H. manillensis from high to low was: beef powder>soybean protein concentrate>plasma protein powder>(pork powder=chicken powder)>(fishmeal>soy protein isolate)>blood meal>hemoglobin>soybean meal>whole milk powder.[Conclusion]The grey correlation degree was above 0.8 for seven kinds of protein feed: beef powder, soybean protein concentrate, plasma protein powder, pork powder, chicken powder, fish meal and soy protein isolate, which can be optimized as the protein feed raw material of H. manillensis feed.
[Purpose]We aimed to study the amino acid balance of the Hirudinaria manillensis and protein feed raw material, in order to provide reference for development of amino acid balanced feed for the leech of the H. manillensis.[Method]The high performance liquid column derivative method was used to determine the content of amino acid in the H. manillensis and 11 protein feedstuffs, in order to evaluate gray correlation degree of amino acids balance between 11 kinds of protein feed raw materials and H. manillensis.[Result]The grey correlation degree of amino acids balance between11 kinds of protein feed raw material and H. manillensis from high to low was: beef powder>soybean protein concentrate>plasma protein powder>(pork powder=chicken powder)>(fishmeal>soy protein isolate)>blood meal>hemoglobin>soybean meal>whole milk powder.[Conclusion]The grey correlation degree was above 0.8 for seven kinds of protein feed: beef powder, soybean protein concentrate, plasma protein powder, pork powder, chicken powder, fish meal and soy protein isolate, which can be optimized as the protein feed raw material of H. manillensis feed.
引文
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[14]林香信,陆鹏,颜孙安,等.无鱼粉蛋白饲料氨基酸平衡对罗非鱼生长的影响[J].农学学报,2012,2(5):62.DOI:10.3969/j.issn.1007-7774.2012.05.013.
[15]雷兴刚,邓君明,麦康森.灰色关联度分析法评价蛋白质营养价值的可行性探讨[J].云南农业大学学报(自然科学),2010,25(4):545.DOI:10.3969/j.issn.1004-390X.2010.04.018.
[16]陈秀梅,申斌,李萌,等.饲料必需氨基酸平衡关联度对框镜鲤生长、蛋白质消化和代谢的影响[J].华南农业大学学报,2015,36(4):36.DOI:10.7671/j.issn.1001-411X.2015.04.007.
[2]周维官,周维海,覃国森.菲牛蛭的人工养殖方式试验[J].广西科学,2008,15(3):317.DOI:10.3969/j.issn.1005-9164.2008.03.031.
[3]于翔.菲牛蛭室内高密度养殖技术研究[D].广州:中山大学,2009.
[4]李军,于翔,龚元,等.正交试验法优选菲牛蛭饲料配方研究[J].中草药,2014,45(6):782.DOI:10.7501/j.issn.0253-2670.2014.06.008.
[5]江龙建,姜勋平,周光宏.应用灰色关联分析评价饲料蛋白质氨基酸平衡性[J].四川畜牧兽医,2001,28(2):20.DOI:10.3969/j.issn.1001-8964.2001.02.015.
[6]陈秀梅,申斌,李萌,等.饲料氨基酸平衡程度对框镜鲤生长和体成分的影响[J].中国饲料,2014(18):28.DOI:10.3969/j.issn.1004-3314.2014.18.010.
[7]刘思峰,党辉国,方志耕.灰色系统理论及其应用[M].开封:河南大学出版社,1991.
[8]申斌,王桂芹,李萌,等.饲料氨基酸平衡程度对框镜鲤生长和饲料利用的影响[J].饲料工业,2014,35(8):25.DOI:10.13302/j.cnki.fi.2014.08.007.
[9]谭芳芳,叶元土,肖顺应,等.补充微囊赖氨酸和蛋氨酸对草鱼生长性能的影响[J].动物营养学报,2010,22(3):804.DOI:10.3969/j.issn.1006-267x.2010.03.042.
[10]汪益峰,周维仁,章世元,等.氨基酸平衡和外源酶对异育银鲫的生长、氮代谢及血液生化指标的影响[J].江苏农业学报,2010,26(1):130.DOI:10.3969/j.issn.1000-4440.2010.01.025.
[11]黄峰.水生动物营养与饲料学[M].北京:化学工业出版社,2011.
[12]GAYLORD T G,BARROWS F T.Multiple amino acid supplementations to reduce dietary protein in plant-based rainbow trout,Oncorhynchus mykiss,feeds[J].Aquaculture,2009,287(1/2):180.
[13]季文娟.黑鲷幼鱼饲料蛋白源氨基酸平衡的研究[J].中国水产科学,2000,7(3):37.DOI:10.3321/j.issn:1005-8737.2000.03.009.
[14]林香信,陆鹏,颜孙安,等.无鱼粉蛋白饲料氨基酸平衡对罗非鱼生长的影响[J].农学学报,2012,2(5):62.DOI:10.3969/j.issn.1007-7774.2012.05.013.
[15]雷兴刚,邓君明,麦康森.灰色关联度分析法评价蛋白质营养价值的可行性探讨[J].云南农业大学学报(自然科学),2010,25(4):545.DOI:10.3969/j.issn.1004-390X.2010.04.018.
[16]陈秀梅,申斌,李萌,等.饲料必需氨基酸平衡关联度对框镜鲤生长、蛋白质消化和代谢的影响[J].华南农业大学学报,2015,36(4):36.DOI:10.7671/j.issn.1001-411X.2015.04.007.
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