底物浓度对玉米秸秆乙醇发酵及残渣甲烷发酵的影响
|
摘要
为研究底物浓度对玉米秸秆乙醇发酵过程中乙醇产率和乙醇发酵剩余残渣厌氧发酵产气特性的影响,在中温(37±0.2)℃条件下,利用实验室自制小型厌氧发酵装置,在底物浓度为2%、3%、4%和5%下开展周期为50 d的序批式厌氧发酵实验,探索不同底物浓度下玉米秸秆发酵乙醇产率和乙醇发酵剩余残渣厌氧发酵产气特性。结果表明:底物浓度对玉米秸秆乙醇发酵影响显著,当底物浓度为3%时,玉米秸秆厌氧发酵乙醇产量最大,达到39.04 g;底物浓度过低或过高均不适合后期厌氧发酵产甲烷的进行,当底物浓度为3%时,玉米秸秆乙醇发酵残渣表面纤维结构被破坏最明显,残渣厌氧发酵产甲烷实验最早在3 d出现产气峰值,挥发性固体单位甲烷产量为26.82 mL·g~(-1),并且累积产气量最高,挥发性固体单位累积甲烷产量达到270.01 mL·g~(-1),玉米秸秆乙醇发酵残渣还有较高的产气潜能;通过质量平衡分析得到,底物浓度为3%时,玉米秸秆生物转化过程中TS和VS去除率最高,分别为59.12%和79.07%。该研究可为玉米秸秆乙醇发酵工程提供参考。
To study the effects of substrate concentration on the ethanol yield in ethanol fermentation of cornstraw and the gas production characteristics in anaerobic fermentation of ethanol fermentation residues, a selfmade and small-scale anaerobic fermentation device was used to conduct the anaerobic fermentationexperiments with sequencing batch mode and 50 day period at medium temperature(37±0.2) ℃ and substrateconcentrations of 2%, 3%, 4% and 5%. The ethanol yield from corn straw ethanol fermentation at differentsubstrate concentrations and the gas production characteristics from following anaerobic fermentation of theresidues of aforementioned ethanol fermentation were explored. The results show that the substrate concentrationhad a significant effect on the ethanol fermentation of corn straw. The maximum ethanol yield from anaerobicfermentation of corn straw was 39.04 g at the substrate concentration of 3%. Too low or high substrateconcentrations were not suitable for the subsequent anaerobic fermentation and methane production. At thesubstrate concentration of 3%, an obvious destruction on the fiber structure of the residue surface after ethanolfermentation of corn straw appeared, and the gas production peak with the corresponding methane productionper volatile solid mass was 26.82 mL·g~(-1)occurred at the third day in the anaerobic fermentation and methaneproduction experiments for the residues. Then the highest cumulative methane production reached 270.01 mL·g~(-1) volatile solid, a high potential for gas production still remained in the residues from ethanol fermentation ofcorn straw. Through mass balance analysis, the highest TS and VS removal rates of 59.12% and 79.07% wereobtained at the substrate concentration of 3% in the corn straw bioconversion. This study can provide a referencefor the ethanol fermentation project of corn straw.
To study the effects of substrate concentration on the ethanol yield in ethanol fermentation of cornstraw and the gas production characteristics in anaerobic fermentation of ethanol fermentation residues, a selfmade and small-scale anaerobic fermentation device was used to conduct the anaerobic fermentationexperiments with sequencing batch mode and 50 day period at medium temperature(37±0.2) ℃ and substrateconcentrations of 2%, 3%, 4% and 5%. The ethanol yield from corn straw ethanol fermentation at differentsubstrate concentrations and the gas production characteristics from following anaerobic fermentation of theresidues of aforementioned ethanol fermentation were explored. The results show that the substrate concentrationhad a significant effect on the ethanol fermentation of corn straw. The maximum ethanol yield from anaerobicfermentation of corn straw was 39.04 g at the substrate concentration of 3%. Too low or high substrateconcentrations were not suitable for the subsequent anaerobic fermentation and methane production. At thesubstrate concentration of 3%, an obvious destruction on the fiber structure of the residue surface after ethanolfermentation of corn straw appeared, and the gas production peak with the corresponding methane productionper volatile solid mass was 26.82 mL·g~(-1)occurred at the third day in the anaerobic fermentation and methaneproduction experiments for the residues. Then the highest cumulative methane production reached 270.01 mL·g~(-1) volatile solid, a high potential for gas production still remained in the residues from ethanol fermentation ofcorn straw. Through mass balance analysis, the highest TS and VS removal rates of 59.12% and 79.07% wereobtained at the substrate concentration of 3% in the corn straw bioconversion. This study can provide a referencefor the ethanol fermentation project of corn straw.
引文
[1]RAJAGOPALAN S,PONNAMPALAM E,MCCALLA D,et al.Enhancing profitability of dry mill ethanol plants[J].Applied Biochemistry&Biotechnology,2005,120(1):37-50.
[2]CUI M,ZHAO L X,TIAN Y S,et al.Analysis and evaluation on energy utilization of main crop straw resources in China[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(12):291-296.
[3]石祖梁,贾涛,王亚静,等.我国农作物秸秆综合利用现状及焚烧碳排放估算[J].中国农业资源与区划,2017,38(9):32-37.
[4]LI X X.Biomass energy:Effective utilization of crop straws[J].Advanced Materials Research,2015,1070-1072:136-139.
[5]KIM D H,KIM S H,KIM H W,et al.Sewage sludge addition to food waste synergistically enhances hydrogen fermentation performance[J].Bioresource Technology,2011,102(18):8501-8506.
[6]廖慧璇,籍永丽,彭少麟.资源环境承载力与区域可持续发展[J].生态环境学报,2016,25(7):1253-1258.
[7]GIUDICIANNI P,CARDONE G,RAGUCCI R.Cellulose,hemicellulose and lignin slow steam pyrolysis:Thermal decomposition of biomass components mixtures[J].Journal of Analytical&Applied Pyrolysis,2013,100:213-222.
[8]杨娟,滕虎,刘海军,等.纤维素乙醇的原料预处理方法及工艺流程研究进展[J].化工进展,2013,32:97-103.
[9]宋安东,任天宝,张百良.玉米秸秆生产燃料乙醇的经济性分析[J].农业工程学报,2010,26(6):283-286.
[10]李世密,魏雅洁,张晓健,等.秸秆类木质纤维素原料厌氧发酵产沼气研究[J].可再生能源,2008,26(1):50-54.
[11]刘丽雪,陈海涛,韩永俊.沼渣物理特性及沼渣纤维化学成分测定与分析[J].农业工程学报,2010,26(7):277-280.
[12]陈玉亮,呼世斌,张守文,等.麦秆分步糖化发酵产乙醇的初步研究[J].西北农业学报,2009,18(2):149-153.
[13]王震,吕喆,杜济良,等.汽爆玉米秸秆乙醇发酵残留物产甲烷特性研究[J].太阳能学报,2016,37(3):542-545.
[14]贾丽娟,俞芳,宁平,等.温度底物浓度和微量元素对牛粪厌氧发酵产沼气的影响[J].农业工程学报,2014,30(22):260-266.
[15]艾平,张济韬,席江,等.纤维素乙醇糟液对稻秸猪粪厌氧发酵的促进机制[J].环境工程学报,2017,11(12):6404-6414.
[16]GILCREAS F W.Standard Methods for the Examination of Water and Wastewater[M].American Public Health Association,1998.
[17]李金平,崔维栋,黄娟娟,等.多元混合物料协同促进厌氧消化产甲烷性能试验研究[J].中国环境科学,2018,38(3):1024-1032.
[18]DA S A,INOUE H,ENDO T,et al.Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation[J].Bioresource Technology,2010,101(19):7402-7409.
[19]胡家骏,周群英.环境工程微生物学[M].北京:高等教育出版社,2008.
[20]TENGBORG C,GALBE M,ZACCHI G.Reduced inhibition of enzymatic hydrolysis of steam-pretreated softwood[J].Enzyme and Microbial Technology,2001,28(9):835-844.
[21]孙晓琦.硬质阔叶木木质纤维素乙醇发酵的研究[D].北京:北京林业大学,2013.
[22]侯保朝,杜风光,郭永豪,等.高浓度酒精发酵[J].酿酒科技,2005(4):93-96.
[23]OHGREN K,BENGTSSON O,GORWAGRAUSLUND M F,et al.Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400[J].Journal of Biotechnology,2006,126(4):488-498.
[24]FAN L T,LEE Y H,BEARDMORE D H.Mechanism of the enzymatic hydrolysis of cellulose:Effects of major structural features of cellulose on enzymatic hydrolysis[J].Biotechnology&Bioengineering,2010,22(1):177-199.
[25]甘荣,葛明民,刘勇迪,等.活性炭在中高温条件下对玉米秸秆厌氧发酵的影响[J].环境科学,2017,38(6):2607-2616.
[26]SUN Y G,MA Y L,WANG L Q,et al.Physicochemical properties of corn stalk after treatment using steam explosion coupled with acid or alkali[J].Carbohydrate Polymers,2015,117:486-493.
[27]陈世平,汤晓玉,肖泽仪,等.玉米秸秆水解残渣厌氧消化的产气性能[J].农业环境科学学报,2016,35(3):584-589.
[28]谢丽萍,赵晓祥,杨虹蛟,等.烟草下脚料发酵制取乙醇[J].环境工程学报,2010,4(6):1417-1420.
[2]CUI M,ZHAO L X,TIAN Y S,et al.Analysis and evaluation on energy utilization of main crop straw resources in China[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(12):291-296.
[3]石祖梁,贾涛,王亚静,等.我国农作物秸秆综合利用现状及焚烧碳排放估算[J].中国农业资源与区划,2017,38(9):32-37.
[4]LI X X.Biomass energy:Effective utilization of crop straws[J].Advanced Materials Research,2015,1070-1072:136-139.
[5]KIM D H,KIM S H,KIM H W,et al.Sewage sludge addition to food waste synergistically enhances hydrogen fermentation performance[J].Bioresource Technology,2011,102(18):8501-8506.
[6]廖慧璇,籍永丽,彭少麟.资源环境承载力与区域可持续发展[J].生态环境学报,2016,25(7):1253-1258.
[7]GIUDICIANNI P,CARDONE G,RAGUCCI R.Cellulose,hemicellulose and lignin slow steam pyrolysis:Thermal decomposition of biomass components mixtures[J].Journal of Analytical&Applied Pyrolysis,2013,100:213-222.
[8]杨娟,滕虎,刘海军,等.纤维素乙醇的原料预处理方法及工艺流程研究进展[J].化工进展,2013,32:97-103.
[9]宋安东,任天宝,张百良.玉米秸秆生产燃料乙醇的经济性分析[J].农业工程学报,2010,26(6):283-286.
[10]李世密,魏雅洁,张晓健,等.秸秆类木质纤维素原料厌氧发酵产沼气研究[J].可再生能源,2008,26(1):50-54.
[11]刘丽雪,陈海涛,韩永俊.沼渣物理特性及沼渣纤维化学成分测定与分析[J].农业工程学报,2010,26(7):277-280.
[12]陈玉亮,呼世斌,张守文,等.麦秆分步糖化发酵产乙醇的初步研究[J].西北农业学报,2009,18(2):149-153.
[13]王震,吕喆,杜济良,等.汽爆玉米秸秆乙醇发酵残留物产甲烷特性研究[J].太阳能学报,2016,37(3):542-545.
[14]贾丽娟,俞芳,宁平,等.温度底物浓度和微量元素对牛粪厌氧发酵产沼气的影响[J].农业工程学报,2014,30(22):260-266.
[15]艾平,张济韬,席江,等.纤维素乙醇糟液对稻秸猪粪厌氧发酵的促进机制[J].环境工程学报,2017,11(12):6404-6414.
[16]GILCREAS F W.Standard Methods for the Examination of Water and Wastewater[M].American Public Health Association,1998.
[17]李金平,崔维栋,黄娟娟,等.多元混合物料协同促进厌氧消化产甲烷性能试验研究[J].中国环境科学,2018,38(3):1024-1032.
[18]DA S A,INOUE H,ENDO T,et al.Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation[J].Bioresource Technology,2010,101(19):7402-7409.
[19]胡家骏,周群英.环境工程微生物学[M].北京:高等教育出版社,2008.
[20]TENGBORG C,GALBE M,ZACCHI G.Reduced inhibition of enzymatic hydrolysis of steam-pretreated softwood[J].Enzyme and Microbial Technology,2001,28(9):835-844.
[21]孙晓琦.硬质阔叶木木质纤维素乙醇发酵的研究[D].北京:北京林业大学,2013.
[22]侯保朝,杜风光,郭永豪,等.高浓度酒精发酵[J].酿酒科技,2005(4):93-96.
[23]OHGREN K,BENGTSSON O,GORWAGRAUSLUND M F,et al.Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400[J].Journal of Biotechnology,2006,126(4):488-498.
[24]FAN L T,LEE Y H,BEARDMORE D H.Mechanism of the enzymatic hydrolysis of cellulose:Effects of major structural features of cellulose on enzymatic hydrolysis[J].Biotechnology&Bioengineering,2010,22(1):177-199.
[25]甘荣,葛明民,刘勇迪,等.活性炭在中高温条件下对玉米秸秆厌氧发酵的影响[J].环境科学,2017,38(6):2607-2616.
[26]SUN Y G,MA Y L,WANG L Q,et al.Physicochemical properties of corn stalk after treatment using steam explosion coupled with acid or alkali[J].Carbohydrate Polymers,2015,117:486-493.
[27]陈世平,汤晓玉,肖泽仪,等.玉米秸秆水解残渣厌氧消化的产气性能[J].农业环境科学学报,2016,35(3):584-589.
[28]谢丽萍,赵晓祥,杨虹蛟,等.烟草下脚料发酵制取乙醇[J].环境工程学报,2010,4(6):1417-1420.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |