脊髓损伤模型大鼠损伤节段的选择:网状Meta分析
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摘要
背景:目前Allen’s打击法已经发展成为一种安全性和有效性相对较好的造模方式,脊髓损伤节段的选择也是建立理想的脊髓损伤模型非常重要的因素。目的:运用网状Meta分析的方法,比较对SD大鼠胸T_8,T_9,T_(10),T_(11),T_(12)造成脊髓损伤有效性的异同,为脊髓损伤大鼠模型损伤节段的选择提供有力证据。方法:计算机检索PubMed、中国知网(cnki)、重庆维普(VIP网)、万方数据库。英文检索主题词:"spinal cord injuries","rats","Models,Animal",中文检索主题词:"脊髓损伤模型","大鼠","BBB评分"。检索国内外所有截止时间2018年6月5日公开发表的所有文献。入选的文献均为Allen’s打击法建立脊髓损伤大鼠模型,损伤量以25 g·cm为标准,以仅进行椎板切除的大鼠为假手术组,采用Basso Beattie Bresnahan(BBB)下肢运动功能评分为观察3,7,14,28 d时间点评分。网状Meta分析比较T_8,T_9,T_(10),T_(11),T_(12)不同节段脊髓损伤有效性的排序。结果与结论:(1)最终纳入研究19篇,共703只大鼠纳入研究;(2)在3,7,14,28 d时间点不同损伤节段脊髓损伤后的BBB评分较假手术组明显下降(P≤0.05);(3)T_(10)节段脊髓损伤对下肢功能BBB评分影响最大、有效性最高;(4)不同损伤节段脊髓损伤的有效性最终的排序是:T_(10)损伤> T_9损伤> T_(11)损伤> T_8损伤> T_(12)损伤>假手术。
BACKGROUND: Allen's weight-drop method has become a safe and effective modeling method, and choosing the injury segments of the spinal cord is an important factor for establishing ideal animal models of spinal cord injury.OBJECTIVE: To compare the the similarities and differences in the effectiveness of spinal cord injury at T_8, T_9, T_(10), T_(11), and T_(12) in Sprague-Dawley rats by network meta-analysis, thus providing evidence for the selection of lesion segments in rat models of spinal cord injury.METHODS: PubMed, CNKI, VIP and WanF ang databases were searched with the keywords of "spinal cord injuries, rats, models, animal,BBB scores" in English and Chinese, respectively for the literature published before June 5, 2018. The inclusion criteria were as follows: the modeling method of spinal cord injury was Allen's weight-drop(25 g·cm) method, rats only undergoing laminectomy were included in sham operation group, and low extremity function was assessed by Basso Beattie Bresnahan scores at 3, 7, 14 and 28 days. The order of the effectiveness of spinal cord injury at T_8, T_9, T_(10), T_(11), and T_(12) was compared by network meta-analysis.RESULTS AND CONCLUSION:(1) Nineteen studies were included, involving 703 rats.(2) Basso Beattie Bresnahan scores in the spinal cord injury rats at different segments at 3, 7, 14 and 28 days were significantly decreased compared with the sham operation group(P ≤ 0.05).(3)Spinal cord injury at T_(10) showed largest effect on Beattie Bresnahan scores.(4) The order of effectiveness of spinal cord injury at different segments was: T_(10) > T_9 > T_(11) > T_8 > T_(12) > sham operation.
BACKGROUND: Allen's weight-drop method has become a safe and effective modeling method, and choosing the injury segments of the spinal cord is an important factor for establishing ideal animal models of spinal cord injury.OBJECTIVE: To compare the the similarities and differences in the effectiveness of spinal cord injury at T_8, T_9, T_(10), T_(11), and T_(12) in Sprague-Dawley rats by network meta-analysis, thus providing evidence for the selection of lesion segments in rat models of spinal cord injury.METHODS: PubMed, CNKI, VIP and WanF ang databases were searched with the keywords of "spinal cord injuries, rats, models, animal,BBB scores" in English and Chinese, respectively for the literature published before June 5, 2018. The inclusion criteria were as follows: the modeling method of spinal cord injury was Allen's weight-drop(25 g·cm) method, rats only undergoing laminectomy were included in sham operation group, and low extremity function was assessed by Basso Beattie Bresnahan scores at 3, 7, 14 and 28 days. The order of the effectiveness of spinal cord injury at T_8, T_9, T_(10), T_(11), and T_(12) was compared by network meta-analysis.RESULTS AND CONCLUSION:(1) Nineteen studies were included, involving 703 rats.(2) Basso Beattie Bresnahan scores in the spinal cord injury rats at different segments at 3, 7, 14 and 28 days were significantly decreased compared with the sham operation group(P ≤ 0.05).(3)Spinal cord injury at T_(10) showed largest effect on Beattie Bresnahan scores.(4) The order of effectiveness of spinal cord injury at different segments was: T_(10) > T_9 > T_(11) > T_8 > T_(12) > sham operation.
引文
[1]陈星月,陈栋,陈春慧,等.中国创伤性脊髓损伤流行病学和疾病经济负担的系统评价[J].中国循证医学杂志,2018,18(2):143-150.
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[9]李康森,黄帅,黄子祥,等.美金刚对急性脊髓损伤大鼠运动功能的影响[J].神经解剖学杂志,2016,32(3):373-379.
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[12]许争光,杨峻,吕志平,等.芫花根醇提颗粒联合甲基泼尼松龙对脊髓损伤大鼠BDNF、NMDA表达及行为学的影响[J].中国中西医结合杂志,2015,35(8):1004-1010.
[13]蒋松鹤,林海燕,何蓉,等.督脉、夹脊电针对脊髓损伤大鼠功能康复的影响[J].中华针灸电子杂志,2015,4(1):7-12.
[14]郭杨,马勇,潘娅岚,等.脊髓康对大鼠脊髓损伤后神经功能恢复及脑源性神经营养因子表达的影响[J].中国康复理论与实践,2014,20(8):701-708.
[15]Kang N,Hai Y,Yang J,et al.Hyperbaric oxygen intervention reduces secondary spinal cord injury in rats via regulation of HMGB1/TLR4/NF-κB signaling pathway.Int J Clin Exp Pathol.2015;8(2):1141-53
[16]Long Y,Liang F,Gao C,et al.Hyperbaric oxygen therapy reduces apoptosis after spinal cord injury in rats.Int J Clin Exp Med.2014;7(11):4073-81
[17]白登彦,张海军,袁治国.局部转染Ngn2基因对大鼠实验性急性脊髓损伤运动功能影响的研究[J].中国伤残医学,2013,21(9):55-57.
[18]贺晓玉.大鼠胸段脊髓打击损伤程度与脊髓功能变化的关系[J].山东医药,2013,53(17):23-25.
[19]宋佳,齐慧慧,贾连顺.原花青素对大鼠急性脊髓损伤的保护作用及机制研究[J].中国矫形外科杂志,2013,21(8):794-798.
[20]王波,方以群,张存海.高压氧对大鼠脊髓损伤后局部炎症因子的影响[J].中国应用生理学杂志,2012,28(5):388-389+393.
[21]李兵奎,曾彬,常巍,等.督脉电针对大鼠脊髓损伤后神经功能恢复的影响[J].中国康复理论与实践,2012,18(7):619-622.
[22]吕越昌.Neuroserpin对大鼠急性脊髓损伤后神经功能的修复作用及其机制的初步研究[D].上海:复旦大学,2013.
[23]杨棋,常巍,李东升,等.大鼠脊髓挫伤模型的建立及其意义?[J].医学临床研究,2015,(8):1471-1473,1474.
[24]付俊文.Butin对大鼠急性脊髓损伤后肢运动功能及NF-κb、Caspase-3因子表达的影响[D].大连:大连医科大学,2012.
[25]刘海润.BMS-345541对大鼠脊髓损伤后粒细胞浸润及运动功能影响的实验研究[D].大连:大连医科大学,2011.
[26]焦杰君,蒋建农,都斌,等.银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的影响[J].实用临床医药杂志,2010,14(15):5-8.
[27]Ropper AE,Zeng X,Anderson JE,et al.An efficient device to experimentally model compression injury of mammalian spinal cord.Exper Neurol.2015;271:515-523.
[28]West H,Richardson WD,Fruttiger M.Stabilization of the retinal vascular network by reciprocal feedback between blood vessels and astrocytes.Development.2005,132(8):1855-62
[29]张富强,刘玮玮,王克平,等.行为学评价大鼠急性脊髓损伤模型脊髓节段选择的研究[J].世界科技研究与发展,2015,37(5):579-583.
[30]尚云龙,李一帆,宁云峰,等.大鼠脊髓损伤模型的解剖学参考定位[J].解剖学研究,2013,35(6):412-414+481.
[31]秦峰,董大明.脊髓损伤模型进展[J].疑难病杂志,2015,14(4):436-439.
[32]张大威,李一帆,朱丹.急性大鼠脊髓损伤模型的建立与评估[J].中风与神经疾病杂志,2015,32(4):325-327.
[33]Bramer WM,Giustini D,Kramer BM.Comparing the coverage,recall,and precision of searches for 120 systematic reviews in Embase,MEDLINE,and Google Scholar:a prospective study.Syst Rev.2016;5:39.
[34]Halladay CW,Trikalinos TA,Schmid IT,et al.Using data sources beyond PubMed has a modest impact on the results of systematic reviews of therapeutic interventions.J Clin Epidemiol.2015;68(9):1076-1084.
[2]Smith E,Fitzpatrick P,Murtagh J,et al.Epidemiology of traumatic spinal cord injury in Ireland,2010-2015.Neuroepidemiology.2018;51(1-2):19-24.
[3]Kumar R,Lim J,Mekary RA,et al.Traumatic spinal injury:global epidemiology and worldwide volume.World Neurosurg.2018;113():e345-e363
[4]Krueger H,Noonan VK,Trenaman LM,et al.The economic burden of traumatic spinal cord injury in Canada.Chronic Dis Inj Can.2013;33(3):113-122.
[5]Varma AK,Das A,Wallace G 4th,et al.Spinal cord injury:a review of current therapy,future treatments,and basic science frontiers.Neurochem Res.2013;38(5):895-905.
[6]van Hedel HJ,Curt A.Fighting for each segment:estimating the clinical value of cervical and thoracic segments in SCI.JNeurotrauma.2006;23(11):1621-1631.
[7]Lundh A,Gotzsche PC.Recommendations by Cochrane Review Groups for assessment of the risk of bias in studies.BMC Med Res Methodol.2008;8:22.
[8]曾欢欢,黄英如,李子健,等.大黄素对大鼠急性脊髓损伤后继发脊髓水肿的影响[J].中国康复理论与实践,2018,24(4):378-384.
[9]李康森,黄帅,黄子祥,等.美金刚对急性脊髓损伤大鼠运动功能的影响[J].神经解剖学杂志,2016,32(3):373-379.
[10]Jia YF,Gao HL,Ma LJ,et al.Effect of nimodipine on rat spinal cord injury.Genet Mol Res.2015;14(1):1269-76
[11]郭磊.大鼠脊髓损伤后β淀粉样蛋白的表达变化及作用机制研究[D].北京:中国人民解放军医学院,2016.
[12]许争光,杨峻,吕志平,等.芫花根醇提颗粒联合甲基泼尼松龙对脊髓损伤大鼠BDNF、NMDA表达及行为学的影响[J].中国中西医结合杂志,2015,35(8):1004-1010.
[13]蒋松鹤,林海燕,何蓉,等.督脉、夹脊电针对脊髓损伤大鼠功能康复的影响[J].中华针灸电子杂志,2015,4(1):7-12.
[14]郭杨,马勇,潘娅岚,等.脊髓康对大鼠脊髓损伤后神经功能恢复及脑源性神经营养因子表达的影响[J].中国康复理论与实践,2014,20(8):701-708.
[15]Kang N,Hai Y,Yang J,et al.Hyperbaric oxygen intervention reduces secondary spinal cord injury in rats via regulation of HMGB1/TLR4/NF-κB signaling pathway.Int J Clin Exp Pathol.2015;8(2):1141-53
[16]Long Y,Liang F,Gao C,et al.Hyperbaric oxygen therapy reduces apoptosis after spinal cord injury in rats.Int J Clin Exp Med.2014;7(11):4073-81
[17]白登彦,张海军,袁治国.局部转染Ngn2基因对大鼠实验性急性脊髓损伤运动功能影响的研究[J].中国伤残医学,2013,21(9):55-57.
[18]贺晓玉.大鼠胸段脊髓打击损伤程度与脊髓功能变化的关系[J].山东医药,2013,53(17):23-25.
[19]宋佳,齐慧慧,贾连顺.原花青素对大鼠急性脊髓损伤的保护作用及机制研究[J].中国矫形外科杂志,2013,21(8):794-798.
[20]王波,方以群,张存海.高压氧对大鼠脊髓损伤后局部炎症因子的影响[J].中国应用生理学杂志,2012,28(5):388-389+393.
[21]李兵奎,曾彬,常巍,等.督脉电针对大鼠脊髓损伤后神经功能恢复的影响[J].中国康复理论与实践,2012,18(7):619-622.
[22]吕越昌.Neuroserpin对大鼠急性脊髓损伤后神经功能的修复作用及其机制的初步研究[D].上海:复旦大学,2013.
[23]杨棋,常巍,李东升,等.大鼠脊髓挫伤模型的建立及其意义?[J].医学临床研究,2015,(8):1471-1473,1474.
[24]付俊文.Butin对大鼠急性脊髓损伤后肢运动功能及NF-κb、Caspase-3因子表达的影响[D].大连:大连医科大学,2012.
[25]刘海润.BMS-345541对大鼠脊髓损伤后粒细胞浸润及运动功能影响的实验研究[D].大连:大连医科大学,2011.
[26]焦杰君,蒋建农,都斌,等.银杏叶提取物EGb761对大鼠脊髓损伤后神经损伤恢复的影响[J].实用临床医药杂志,2010,14(15):5-8.
[27]Ropper AE,Zeng X,Anderson JE,et al.An efficient device to experimentally model compression injury of mammalian spinal cord.Exper Neurol.2015;271:515-523.
[28]West H,Richardson WD,Fruttiger M.Stabilization of the retinal vascular network by reciprocal feedback between blood vessels and astrocytes.Development.2005,132(8):1855-62
[29]张富强,刘玮玮,王克平,等.行为学评价大鼠急性脊髓损伤模型脊髓节段选择的研究[J].世界科技研究与发展,2015,37(5):579-583.
[30]尚云龙,李一帆,宁云峰,等.大鼠脊髓损伤模型的解剖学参考定位[J].解剖学研究,2013,35(6):412-414+481.
[31]秦峰,董大明.脊髓损伤模型进展[J].疑难病杂志,2015,14(4):436-439.
[32]张大威,李一帆,朱丹.急性大鼠脊髓损伤模型的建立与评估[J].中风与神经疾病杂志,2015,32(4):325-327.
[33]Bramer WM,Giustini D,Kramer BM.Comparing the coverage,recall,and precision of searches for 120 systematic reviews in Embase,MEDLINE,and Google Scholar:a prospective study.Syst Rev.2016;5:39.
[34]Halladay CW,Trikalinos TA,Schmid IT,et al.Using data sources beyond PubMed has a modest impact on the results of systematic reviews of therapeutic interventions.J Clin Epidemiol.2015;68(9):1076-1084.
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