活性黑染料脱色菌的分离鉴定及复合菌群培养研究
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摘要
从活性污泥中分离得到6株活性黑脱色菌,从中筛选出脱色能力最强的菌株Y1,经菌落形态、生理生化特性及16S r RNA基因序列分析,鉴定该菌株属于希瓦氏菌属(Shewanella).通过两两复配、三三复配实验探究复合菌群的脱色效果,结果表明,混合培养后复合菌群对活性黑染料的脱色率较单一菌株培养时均有较明显的提高.部分复合菌群的脱色率在72 h后稳定在90%以上,其中复合菌株Y1+Y5的脱色率最高为95. 68%,并且其对印染工业废水也具有较强的脱色能力(脱色率为92. 69%).
Six strains of bacteria with capacity of decolorizing dyes were isolated from activated sludge.Strain Y1,with excellent ability of dye-decolorizing,was identified as Shewanella sp. based on morphological and physiological-biochemical characteristics and 16 S r RNA gene sequence analysis. After mixed cultivating,the decolorizing rate of reactive black dye was obviously improved,and the decolorizing rate of most multiple colonies steadied at more than 90% after 72 h. It was found that the decolorizing rate of multiple colony Y1 +Y5 was 95. 68%,and it has excellent ability of dye-decolorizing for industrial printing and dying wastewater( decolorizing rate is 92. 69%).
Six strains of bacteria with capacity of decolorizing dyes were isolated from activated sludge.Strain Y1,with excellent ability of dye-decolorizing,was identified as Shewanella sp. based on morphological and physiological-biochemical characteristics and 16 S r RNA gene sequence analysis. After mixed cultivating,the decolorizing rate of reactive black dye was obviously improved,and the decolorizing rate of most multiple colonies steadied at more than 90% after 72 h. It was found that the decolorizing rate of multiple colony Y1 +Y5 was 95. 68%,and it has excellent ability of dye-decolorizing for industrial printing and dying wastewater( decolorizing rate is 92. 69%).
引文
[1]刘梅红.印染废水处理技术研究进展[J].纺织学报,2007,1(28):116-119.
[2]赵鸭美,谢柳,周俊利.一株广谱高效脱色菌的分离鉴定及其脱色特性[J].基因组学与应用生物学,2016,35(1):130-136.
[3]章杰.禁用染料和环保型染料[M].北京:中国化学工业出版社,2001:117.
[4]胥维昌.染料行业废水处理现状和展望[J].染料工业,2002,39(6):35-39.
[5]张宁,邹华,吴倩云.零价铝/酸体系对活性黑染料的降解[J].环境工程学报,2014,8(12):5237-5244.
[6]邱丽娟,陈亮,黄满红,等.3种污泥对活性黑KN-B的生物吸附及降解研究[J].环境工程学报,2010,4(1):96-100.
[7]任南琪,周显娇,郭婉茜,等.染料废水处理技术研究进展[J].化工学报,2013,64(1):84-94.
[8]Aravindhan R,Rao J R,Nair B U.Removal of basic yellow dye from aqueous solution by sorption on green alga Caulerpa scalpelliformis[J].Journal of Hazardous Materials,2007,142:68-76.
[9]Sarioglu M,Bali U,Bisgin T.The removal of C.I.Basic Red 46 in a mixed methanohenic anaerobic culture[J].Dyes and Pigments,2007,74:223-229.
[10]龚为进,刘玥.印染废水生物脱色研究进展[J].河南化工,2012,6(29):17-20.
[11]李庄,曾光明,高兴斋,等.偶氮染料废水处理研究现状及其发展方向[J].湖南化工,2000,30(6):12-15.
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[14]花莉,解井坤,朱超,等.微生物对偶氮染料脱色降解的研究进展[J].西北农林科技大学学报(自然科学版),2015,43(10):104-109.
[15]陈晔,陈刚,陈亮,等.活性黑5高效脱色菌的分离鉴定和生长特性研究[J].环境科学与技术,2011,34(2):64-68.
[16]Khehraa M S,Sainia H S,Sharma D K,et al.Decolorization of various azo dyes by bacterial consortium[J].Dyes and Pigments,2005,67(1):55-61.
[17]叶红曼.染料脱色优势菌的脱色特性及配伍特性研究[D].合肥:合肥工业大学博士论文,2011:50-53.
[18]杨清香,贾振杰.微生物染料脱色研究进展[J].微生物学通报,2006,33(4):144-148.
[19]谢学辉,朱玲玉,刘娜,等.印染废水处理功能菌研究进展[J].化工进展,2015,24(2):554-559.
[20]许玫英,钟小燕,曹渭,等.脱色希瓦氏菌(Shewanella decolorationis)S12T的脱色特性[J].微生物学通报,2005,32(1):5-9.
[21]江莹,桂震,齐杭杭,等.一株腐败希瓦氏菌的筛选鉴定及其染料脱色研究[J].安徽农业科学,2015,43(23):224-228.
[22]Ji Q Y,Liu G F,Zhou J T,et al.Removal of water-insoluble Sudan dyes by Shewanella oneidensis MR-1[J].Bioresource Technology,2012,114:144-148.
[23]Liu G F,Zhou J T,Wang J,et al.Decolorization of azo dyes by Shewanella oneidensis MR-1 in the presence of humic acid[J].Applied Microbiology and Biotechnology,2011,91(2):417-424.
[24]Yang Y Y,Du L N,Wang G,et al.The decolorization capacity and mechanism of Shewanella oneidensis MR-1 for methyl orange and Acid Yellow 199 under microaerophilic conditions[J].Water Science and Technology,2011,63(5):957-964.
[25]Wu J,Kim K,Sung N C,et al.Isolation and characterization of Shewanella oneidensis WL-7 capable of decolorizing azo dye reactive black 5[J].The Journal of General and Applied Microbiology,2009,55(1):51-55.
[26]刘亮,刘聪,蒋继宏,等.希瓦氏菌在印染废水脱色中的研究进展[J].环境科学与技术,2015,43(6):110-116.
[27]沈永红,薛玉,刘与鹏,等.高效微生物絮凝剂产生菌的筛选及复合菌群培养研究[J].河南大学学报(自然科学版),2015,45(3):334-338.
[28]张建恒,霍元子,王阳阳,等.浒苔与球等鞭金藻相互抑制的实验验证[J].上海海洋大学学报,2011,20(2):211-216.
[2]赵鸭美,谢柳,周俊利.一株广谱高效脱色菌的分离鉴定及其脱色特性[J].基因组学与应用生物学,2016,35(1):130-136.
[3]章杰.禁用染料和环保型染料[M].北京:中国化学工业出版社,2001:117.
[4]胥维昌.染料行业废水处理现状和展望[J].染料工业,2002,39(6):35-39.
[5]张宁,邹华,吴倩云.零价铝/酸体系对活性黑染料的降解[J].环境工程学报,2014,8(12):5237-5244.
[6]邱丽娟,陈亮,黄满红,等.3种污泥对活性黑KN-B的生物吸附及降解研究[J].环境工程学报,2010,4(1):96-100.
[7]任南琪,周显娇,郭婉茜,等.染料废水处理技术研究进展[J].化工学报,2013,64(1):84-94.
[8]Aravindhan R,Rao J R,Nair B U.Removal of basic yellow dye from aqueous solution by sorption on green alga Caulerpa scalpelliformis[J].Journal of Hazardous Materials,2007,142:68-76.
[9]Sarioglu M,Bali U,Bisgin T.The removal of C.I.Basic Red 46 in a mixed methanohenic anaerobic culture[J].Dyes and Pigments,2007,74:223-229.
[10]龚为进,刘玥.印染废水生物脱色研究进展[J].河南化工,2012,6(29):17-20.
[11]李庄,曾光明,高兴斋,等.偶氮染料废水处理研究现状及其发展方向[J].湖南化工,2000,30(6):12-15.
[12]徐文东,文湘华.微生物在含染料废水处理中的应用[J].环境污染治理技术与设备,2000,1(2):9-16.
[13]洪义国,许玫英,郭俊,等.细菌偶氮还原研究进展[J].应用与环境生物学报,2005,11(5):642-647.
[14]花莉,解井坤,朱超,等.微生物对偶氮染料脱色降解的研究进展[J].西北农林科技大学学报(自然科学版),2015,43(10):104-109.
[15]陈晔,陈刚,陈亮,等.活性黑5高效脱色菌的分离鉴定和生长特性研究[J].环境科学与技术,2011,34(2):64-68.
[16]Khehraa M S,Sainia H S,Sharma D K,et al.Decolorization of various azo dyes by bacterial consortium[J].Dyes and Pigments,2005,67(1):55-61.
[17]叶红曼.染料脱色优势菌的脱色特性及配伍特性研究[D].合肥:合肥工业大学博士论文,2011:50-53.
[18]杨清香,贾振杰.微生物染料脱色研究进展[J].微生物学通报,2006,33(4):144-148.
[19]谢学辉,朱玲玉,刘娜,等.印染废水处理功能菌研究进展[J].化工进展,2015,24(2):554-559.
[20]许玫英,钟小燕,曹渭,等.脱色希瓦氏菌(Shewanella decolorationis)S12T的脱色特性[J].微生物学通报,2005,32(1):5-9.
[21]江莹,桂震,齐杭杭,等.一株腐败希瓦氏菌的筛选鉴定及其染料脱色研究[J].安徽农业科学,2015,43(23):224-228.
[22]Ji Q Y,Liu G F,Zhou J T,et al.Removal of water-insoluble Sudan dyes by Shewanella oneidensis MR-1[J].Bioresource Technology,2012,114:144-148.
[23]Liu G F,Zhou J T,Wang J,et al.Decolorization of azo dyes by Shewanella oneidensis MR-1 in the presence of humic acid[J].Applied Microbiology and Biotechnology,2011,91(2):417-424.
[24]Yang Y Y,Du L N,Wang G,et al.The decolorization capacity and mechanism of Shewanella oneidensis MR-1 for methyl orange and Acid Yellow 199 under microaerophilic conditions[J].Water Science and Technology,2011,63(5):957-964.
[25]Wu J,Kim K,Sung N C,et al.Isolation and characterization of Shewanella oneidensis WL-7 capable of decolorizing azo dye reactive black 5[J].The Journal of General and Applied Microbiology,2009,55(1):51-55.
[26]刘亮,刘聪,蒋继宏,等.希瓦氏菌在印染废水脱色中的研究进展[J].环境科学与技术,2015,43(6):110-116.
[27]沈永红,薛玉,刘与鹏,等.高效微生物絮凝剂产生菌的筛选及复合菌群培养研究[J].河南大学学报(自然科学版),2015,45(3):334-338.
[28]张建恒,霍元子,王阳阳,等.浒苔与球等鞭金藻相互抑制的实验验证[J].上海海洋大学学报,2011,20(2):211-216.
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