毛枝藻对人工污水脱氮除磷能力的研究
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摘要
文章探究了2株毛枝藻(Stigeoclonium sp.)SHY-370及HB1617在不同初始氨氮浓度以及不同氮磷比条件下的生长情况与氮磷去除能力。结果表明氨氮浓度对2株毛枝藻的生长及TP去除能力均有一定的影响, SHY-370可耐受最大氨氮浓度为10 mg/L, HB1617为5 mg/L;氨氮浓度为1—10 mg/L时SHY-370及HB1617对其去除率均达到97%以上,最大去除速率为3.98 mg/(L·d)。氮磷比对SHY-370的生长影响不大,但在氮磷比大于20时HB1617的生长受到抑制; SHY-370对NO_3~--N去除的最佳氮磷比为10—40, HB1617为2—10;氮磷比为2时水体中TP的含量超过了SHY-370及HB1617所能去除的最大值,去除率较低。实验结果表明SHY-370及HB1617在污水深度脱氮除磷方面具有一定的潜力,可考虑将其应用于城市生活污水二级出水(TN≤15 mg/L、TP≤0.5 mg/L、 NH_4~+-N≤5 mg/L)的深度处理。
In this study, we explored the ability to remove nitrate and phosphorus of two strains of Stigeoclonium spp.(SHY-370 and HB1617) by setting different initial concentrations of ammonia nitrogen and nitrogen/phosphorus(N/P)ratios in synthetic wastewater. The results indicated that different initial concentrations of ammonia nitrogen in synthetic wastewater could affect the biomass concentration and the ability to remove N and P of SHY-370 and HB1617. The maximum ammonia nitrogen concentration in synthetic wastewater that SHY-370 can tolerate was 10 mg/L, while that was 5 mg/L for HB1617. The ammonia-nitrogen removal rate of SHY-370 and HB1617 was above 97% and the maximum daily ammonia-nitrogen removal rate was 3.98 mg/L/d, while the initial concentration of ammonia nitrogen in synthetic wastewater varied between 1-10 mg/L. N/P ratios had a slight impact on the biomass concentration of the SHY-370, however, the biomass concentration of HB1617 was reduced when the corresponding rate was over 20 mg/L.The concentration of total phosphorus(TP) in synthetic wastewater was 10 mg/L. When the N/P ratio was 2, the content of TP in synthetic wastewater was higher than 10 mg/L, which led to a low TP removal rate. According to the results of our study, we can conclude that SHY-370 and HB1617 are appropriate for the treatment of urban sewage in which the concentration of ammonia nitrogen is relatively low, whereas the concentration of TP and NO_3~-N is relatively high.
In this study, we explored the ability to remove nitrate and phosphorus of two strains of Stigeoclonium spp.(SHY-370 and HB1617) by setting different initial concentrations of ammonia nitrogen and nitrogen/phosphorus(N/P)ratios in synthetic wastewater. The results indicated that different initial concentrations of ammonia nitrogen in synthetic wastewater could affect the biomass concentration and the ability to remove N and P of SHY-370 and HB1617. The maximum ammonia nitrogen concentration in synthetic wastewater that SHY-370 can tolerate was 10 mg/L, while that was 5 mg/L for HB1617. The ammonia-nitrogen removal rate of SHY-370 and HB1617 was above 97% and the maximum daily ammonia-nitrogen removal rate was 3.98 mg/L/d, while the initial concentration of ammonia nitrogen in synthetic wastewater varied between 1-10 mg/L. N/P ratios had a slight impact on the biomass concentration of the SHY-370, however, the biomass concentration of HB1617 was reduced when the corresponding rate was over 20 mg/L.The concentration of total phosphorus(TP) in synthetic wastewater was 10 mg/L. When the N/P ratio was 2, the content of TP in synthetic wastewater was higher than 10 mg/L, which led to a low TP removal rate. According to the results of our study, we can conclude that SHY-370 and HB1617 are appropriate for the treatment of urban sewage in which the concentration of ammonia nitrogen is relatively low, whereas the concentration of TP and NO_3~-N is relatively high.
引文
[1]Abdel-Raouf N,Al-Homaidan A A,Ibraheem I B M.Mi-croalgae and wastewater treatment[J].Saudi Journal of Biological Sciences,2012,19(3):257-275
[2]Cai T,Park S Y,Li Y.Nutrient recovery from wastewater streams by microalgae:status and prospects[J].Renewable and Sustainable Energy Reviews,2013,19(1):360-369
[3]Wang L,Li Y,Chen P,et al.Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.[J].Bioresource Technology,2010,101(8):2623-2628
[4]González L E,Ca?izares R O,Baena S.Efficiency of ammonia and phosphorus removal from a Colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus[J].Bioresource Technology,1997,60(3):259-262
[5]Kong Q,Li L,Martinez B,et al.Culture of microalgae Chlamydomonas reinhardtii in wastewater for biomass feedstock production[J].Applied Biochemistry and Biotechnology,2010,160(1):9-18
[6]Kuang Q J,Ma P M,Liu G X,et al.Study on the removal efficiency of nitrogen and phosphorus by filamentous green algae[J].Acta Hydrobiologica Sinica,2004,28(3):323-326[况琪军,马沛明,刘国祥,等.大型丝状绿藻对N,P去除效果研究.水生生物学报,2004,28(3):323-326]
[7]Harding J P C,Whitton B A.Resistance to zinc of Stigeoclonium tenue in the field and the laboratory[J].British Phycological Journal,1976,11(4):417-426
[8]Pawlik-Skowrońska B.Phytochelatin production in freshwater algae Stigeoclonium in response to heavy metals contained in mining water;effects of some environmental factors[J].Aquatic Toxicology,2001,52(3-4):241-249
[9]Liu J,Danneels B,Vanormelingen P,et al.Nutrient removal from horticultural wastewater by benthic filamentous algae Klebsormidium sp.,Stigeoclonium spp.and their communities:From laboratory flask to outdoor Algal Turf Scrubber(ATS)[J].Water Research,2016,92:61-68
[10]Xin L,Hong-ying H,Ke G,et al.Effects of different nitrogen and phosphorus concentrations on the growth,nutrient uptake,and lipid accumulation of a freshwater microalga Scenedesmus sp.[J].Bioresource Technology,2010,101(14):5494-5500
[11]Li J P,Wu L B,DaiY K,et al.Effects of different nitrogen-phosphorus ratio on the freshwater phytoplankton growth and the variations of environmental factors[J].Ecology and Environment,2007,16(2):342-346[李建平,吴立波,戴永康,等.不同氮磷比对淡水藻类生长的影响及水环境因子的变化.生态环境,2007,16(2):342-346]
[12]Rhee G Y,Gotham I J.Optimum N:P ratios and coexistence of planktonic algae[J].Journal of Phycology,1980,16(4):486-489
[13]Liu L L,Huang X X,Wei L K,et al.Removal of nitrogen and phosphorus by 15 strains of microalgae and their nutritional values in piggery sewage[J].Acta ScientiaeCircumstantiae,2014,34(8):1986-1994[刘林林,黄旭雄,危立坤,等.15株微藻对猪场养殖污水中氮磷的净化及其细胞营养分析.环境科学学报,2014,34(8):1986-1994]
[14]Chen C Y,Zhuang Y Y,Fang S Q.Study on N、P removed on culturing wastewater by Chlorella[J].Marine Environmental Science,2009,28(1):9-11[陈春云,庄源益,方圣琼.小球藻对养殖废水中N,P的去除研究.海洋环境科学,2009,28(1):9-11]
[15]State Bureau of Technical Supervision.PRC National Standard:Water Quality-Determination of Nitrate-nitrogen-Ultraviolet Spectrophotometry(HJ/T 346-2007)[S].Beijing:China Standards Press.2007[国家技术监督局.中华人民共和国国家标准:水质硝酸盐氮的测定紫外分光光度法(HJ/T 346-2007).北京:中国标准出版社.2007]
[16]State Bureau of Technical Supervision.PRC National Standard:Water Quality-Determination of Total Phosphorus-Ammonium Molybdate Spectrophotometric Method(GB 11893-89)[S].Beijing:China Standards Press.1990[国家技术监督局.中华人民共和国国家标准:水质总磷的测定钼酸铵分光光度法(GB 11893-89).北京:中国标准出版社.1990]
[17]State Bureau of Technical Supervision.PRC National Standard:Water Quality―Determination of Ammonia Nitrogen―Salicylic Acid Spectrophotometry(HJ 536-2009)[S].Beijing:China Standards Press.2010[国家技术监督局.中华人民共和国国家标准:水质氨氮的测定水杨酸分光光度法(HJ 536-2009).北京:中国标准出版社.2010]
[18]Xin L,Hong-ying H,Ke G,et al.Growth and nutrient re-moval properties of a freshwater microalga Scenedesmus sp.LX1 under different kinds of nitrogen sources[J].Ecological Engineering,2010,36(4):379-381
[19]Geider R J,La Roche J.Redfield revisited:variability of C:N:P in marine microalgae and its biochemical basis[J].European Journal of Phycology,2002,37(1):1-17
[20]Liang J J,Jiang X M,Jiang M W,et al.Study on removal rate of NH+4-N and PO3{4-P by immobilized Nannochloropsis oculata[J].Acta Hydrobiologica Sinica,2016,40(5):1033-1040[梁晶晶,蒋霞敏,蒋茂旺等.固定化微绿球藻去除NH+4-N、PO3{4-P效果的研究.水生生物学报,2016,40(5):1033-1040]
[21]Liu J,Vyverman W.Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp.,Klebsormidium sp.and Pseudanabaena sp.under varying N/P conditions[J].Bioresource Technology,2015,179:234-242
[22]Zhao X F,Ma P M,Liu G X,et al.Status of Chlorella sp.and Microcystis aeruginosa in different phosphorus concentrations[J].Environmental Science&Technology,2013,36(11):1-6[赵先富,马沛明,刘国祥,等.不同磷浓度对小球藻铜绿微囊藻生长及生理的影响.环境科学与技术,2013,36(11):1-6]
[23]Feng M W,Wu Y H,Feng S X,et al.Effect of different N/P ratios on algal growth[J].Ecology and Environment,2008,17(5):1759-1763[丰茂武,吴云海,冯仕训,等.不同氮磷比对藻类生长的影响.生态环境,2008,17(5):1759-1763]
[24]Aslan S,Kapdan I K.Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae[J].Ecological Engineering,2006,28(1):64-70
[2]Cai T,Park S Y,Li Y.Nutrient recovery from wastewater streams by microalgae:status and prospects[J].Renewable and Sustainable Energy Reviews,2013,19(1):360-369
[3]Wang L,Li Y,Chen P,et al.Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.[J].Bioresource Technology,2010,101(8):2623-2628
[4]González L E,Ca?izares R O,Baena S.Efficiency of ammonia and phosphorus removal from a Colombian agroindustrial wastewater by the microalgae Chlorella vulgaris and Scenedesmus dimorphus[J].Bioresource Technology,1997,60(3):259-262
[5]Kong Q,Li L,Martinez B,et al.Culture of microalgae Chlamydomonas reinhardtii in wastewater for biomass feedstock production[J].Applied Biochemistry and Biotechnology,2010,160(1):9-18
[6]Kuang Q J,Ma P M,Liu G X,et al.Study on the removal efficiency of nitrogen and phosphorus by filamentous green algae[J].Acta Hydrobiologica Sinica,2004,28(3):323-326[况琪军,马沛明,刘国祥,等.大型丝状绿藻对N,P去除效果研究.水生生物学报,2004,28(3):323-326]
[7]Harding J P C,Whitton B A.Resistance to zinc of Stigeoclonium tenue in the field and the laboratory[J].British Phycological Journal,1976,11(4):417-426
[8]Pawlik-Skowrońska B.Phytochelatin production in freshwater algae Stigeoclonium in response to heavy metals contained in mining water;effects of some environmental factors[J].Aquatic Toxicology,2001,52(3-4):241-249
[9]Liu J,Danneels B,Vanormelingen P,et al.Nutrient removal from horticultural wastewater by benthic filamentous algae Klebsormidium sp.,Stigeoclonium spp.and their communities:From laboratory flask to outdoor Algal Turf Scrubber(ATS)[J].Water Research,2016,92:61-68
[10]Xin L,Hong-ying H,Ke G,et al.Effects of different nitrogen and phosphorus concentrations on the growth,nutrient uptake,and lipid accumulation of a freshwater microalga Scenedesmus sp.[J].Bioresource Technology,2010,101(14):5494-5500
[11]Li J P,Wu L B,DaiY K,et al.Effects of different nitrogen-phosphorus ratio on the freshwater phytoplankton growth and the variations of environmental factors[J].Ecology and Environment,2007,16(2):342-346[李建平,吴立波,戴永康,等.不同氮磷比对淡水藻类生长的影响及水环境因子的变化.生态环境,2007,16(2):342-346]
[12]Rhee G Y,Gotham I J.Optimum N:P ratios and coexistence of planktonic algae[J].Journal of Phycology,1980,16(4):486-489
[13]Liu L L,Huang X X,Wei L K,et al.Removal of nitrogen and phosphorus by 15 strains of microalgae and their nutritional values in piggery sewage[J].Acta ScientiaeCircumstantiae,2014,34(8):1986-1994[刘林林,黄旭雄,危立坤,等.15株微藻对猪场养殖污水中氮磷的净化及其细胞营养分析.环境科学学报,2014,34(8):1986-1994]
[14]Chen C Y,Zhuang Y Y,Fang S Q.Study on N、P removed on culturing wastewater by Chlorella[J].Marine Environmental Science,2009,28(1):9-11[陈春云,庄源益,方圣琼.小球藻对养殖废水中N,P的去除研究.海洋环境科学,2009,28(1):9-11]
[15]State Bureau of Technical Supervision.PRC National Standard:Water Quality-Determination of Nitrate-nitrogen-Ultraviolet Spectrophotometry(HJ/T 346-2007)[S].Beijing:China Standards Press.2007[国家技术监督局.中华人民共和国国家标准:水质硝酸盐氮的测定紫外分光光度法(HJ/T 346-2007).北京:中国标准出版社.2007]
[16]State Bureau of Technical Supervision.PRC National Standard:Water Quality-Determination of Total Phosphorus-Ammonium Molybdate Spectrophotometric Method(GB 11893-89)[S].Beijing:China Standards Press.1990[国家技术监督局.中华人民共和国国家标准:水质总磷的测定钼酸铵分光光度法(GB 11893-89).北京:中国标准出版社.1990]
[17]State Bureau of Technical Supervision.PRC National Standard:Water Quality―Determination of Ammonia Nitrogen―Salicylic Acid Spectrophotometry(HJ 536-2009)[S].Beijing:China Standards Press.2010[国家技术监督局.中华人民共和国国家标准:水质氨氮的测定水杨酸分光光度法(HJ 536-2009).北京:中国标准出版社.2010]
[18]Xin L,Hong-ying H,Ke G,et al.Growth and nutrient re-moval properties of a freshwater microalga Scenedesmus sp.LX1 under different kinds of nitrogen sources[J].Ecological Engineering,2010,36(4):379-381
[19]Geider R J,La Roche J.Redfield revisited:variability of C:N:P in marine microalgae and its biochemical basis[J].European Journal of Phycology,2002,37(1):1-17
[20]Liang J J,Jiang X M,Jiang M W,et al.Study on removal rate of NH+4-N and PO3{4-P by immobilized Nannochloropsis oculata[J].Acta Hydrobiologica Sinica,2016,40(5):1033-1040[梁晶晶,蒋霞敏,蒋茂旺等.固定化微绿球藻去除NH+4-N、PO3{4-P效果的研究.水生生物学报,2016,40(5):1033-1040]
[21]Liu J,Vyverman W.Differences in nutrient uptake capacity of the benthic filamentous algae Cladophora sp.,Klebsormidium sp.and Pseudanabaena sp.under varying N/P conditions[J].Bioresource Technology,2015,179:234-242
[22]Zhao X F,Ma P M,Liu G X,et al.Status of Chlorella sp.and Microcystis aeruginosa in different phosphorus concentrations[J].Environmental Science&Technology,2013,36(11):1-6[赵先富,马沛明,刘国祥,等.不同磷浓度对小球藻铜绿微囊藻生长及生理的影响.环境科学与技术,2013,36(11):1-6]
[23]Feng M W,Wu Y H,Feng S X,et al.Effect of different N/P ratios on algal growth[J].Ecology and Environment,2008,17(5):1759-1763[丰茂武,吴云海,冯仕训,等.不同氮磷比对藻类生长的影响.生态环境,2008,17(5):1759-1763]
[24]Aslan S,Kapdan I K.Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae[J].Ecological Engineering,2006,28(1):64-70
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