一株磺化钻井液降解菌的筛选鉴定及其降解特性

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英文篇名：Screening, evaluation and degradation property of the degrading bacteria suitable for sulphonated drilling fluid 作者：宋淑芬 ; 马立安 ; 胡传炯 ; 张照明 ; 周宏 ; 余维初 英文作者：SONG Shufen;MA Li' an;HU Chuanjiong;ZHANG Zhaoming;ZHOU Hong;YU Weichu;State Key Laboratory of Petroleum Pollution Control;Yangtze University;Hubei Collaborative Innovation Center for Unconventional Oil and Gas;Dongying City Kewei Biotechnology L.td;Beijing Design Branch of China Petroleum Engineering Construction Co., Ltd; 关键词：磺化钻井液 ; 降解菌 ; 筛选 ; 降解特性 ; 弯曲芽孢杆菌 ; 钻井废液 ; 无害化处理 英文关键词：sulphonated drilling fluid;;Degrading bacteria;;screening;;degradation characteristic;;Bacillus flexus;;waste drilling fluid;;harmless treatment 中文刊名：SYZC 英文刊名：Oil Drilling & Production Technology 机构：石油石化污染物控制与处理国家重点实验室;长江大学;非常规油气湖北省协同创新中心;科维生物技术有限公司;中国石油工程建设有限公司; 出版日期：2018-09-20 出版单位：石油钻采工艺 年：2018 期：v.40;No.239 基金：石油石化污染物控制与处理国家重点实验室开放课题“聚磺钻井液废液及钻屑土壤化作用机理研究”(编号:PPC2016004) 语种：中文; 页：SYZC201805010 页数：7 CN：05 ISSN：13-1072/TE 分类号：55-61

摘要

为了筛选磺化钻井液高效降解菌株,以新疆某磺化钻井液伤害土壤为菌源,磺化钻井液为唯一碳源,通过富集驯化分离筛选出一株降解磺化钻井液效率较高的菌株Xh8,经鉴定为Bacillus flexus。经生理生化及16S rRNA基因序列分析确定了其分类地位,并进行了单因素和正交试验以确定其降解特性。单因素试验表明Xh8生长最佳条件为:pH值为9.5、盐质量分数为4%、转速为150 r/min、温度为35℃、接种量为10%、N源蛋白胨、P源K2HPO4。正交试验表明磺化钻井液COD降解最佳条件为:pH值为9.5、盐质量分数为4.5%、转速为150 r/min、温度为35℃。培养7 d,磺化钻井液COD降解率为22.9%。试验结果表明,Xh8可作为磺化钻井液降解的优良备选菌株。该研究可为进一步治理受到磺化钻井液伤害的地层提供理论指导。
        In order to screen out the bacteria strain that can degrade sulphonated drilling fluid efficiently, a series of studies were carried out with the sulphonated drilling fluid as the only carbon source and some soil contaminated by the sulphonated drilling fluid in Xinjiang as the bacteria source. The bacteria strain Xh8 that can degrade sulphonated drilling fluid more efficiently was screened out by means of enrichment, acclimation and separation. And according to the evaluation, it is Bacillus flexus. Then, its classification status was determined by virtue of physiological & biochemical and 16 S rRNA gene sequence analysis. In addition, its degradation property was figured out through single-factor experiment and orthogonal experiment. The single-factor experiment results show that the growth of Xh8 is optimal in the condition of pH value 9.5, salt mass fraction 4%, rotation speed 150 r/min, temperature 35 ℃, inoculation amount 10%, N-source peptone, and P-source K2 HPO4. The orthogonal experiment results show that the optimal COD degradation condition of sulphonated drilling fluid is pH value 9.5, salt mass fraction 4.5%, rotation speed 150 r/min and temperature 35 ℃. After being cultured for 7 days, the COD degradation rate of sulphonated drilling fluid is 22.9%. The experimental results indicate that Xh8 can be taken as the preferred bacteria strain for the degradation of sulphonated drilling fluid. The research results can provide the theoretical guidance for the further treatment of the strata contaminated by sulphonated drilling fluid.

引文

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