双修饰Fe_3O_4/CdSe/CdS荧光磁性复合物的制备及其表征
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摘要
采用共沉淀法制得Fe3O4溶胶,用γ-缩水甘油醚氧丙基三甲氧基硅烷(KH-560)对其表面进行改性,制得有机硅改性的纳米Fe3O4磁性粒子;用L-半胱氨酸盐酸盐(L-Cys)将油相的CdSe/CdS转成水相并带上氨基的CdSe/CdS纳米晶;将其复合制备了Fe3O4/CdSe/CdS荧光磁性双功能纳米复合物颗粒。该Fe3O4/CdSe/CdS复合物颗粒平均尺寸约为40nm,饱和磁化强度为21.287A.m2/kg,该纳米粒子既具有优异的荧光特性,也具有较强的超顺磁性。
Fe3O4 magnetic nanoparticles were prepared using hydrothermal coprecipitation process of ferric and ferrous ions.The as-prepared nanoparticles were further modified by γ-(2,3-epoxypropoxy)propytrimethoxysilane(KH-560) to obtain silicone modified Fe3O4 nanoparticles.Also,the water-soluble CdSe/CdS bearing amino have been synthesized using L-cysteine hydrochloride(L-Cys) as stabilizer,At last,CdSe/CdS were linked to Fe3O4 to form luminescent/magnetic bifunctional nanoparticles.The as-prepared composites with a typical diameter of 40nm show 21.287A·m2/kg of saturation magnetic susceptibility.Such material still retains a strong photoluminescence effect of CdSe/CdS quantum dots and also exhibits a typical superparamagnetic behavior of Fe3O4 nanoparticles.
Fe3O4 magnetic nanoparticles were prepared using hydrothermal coprecipitation process of ferric and ferrous ions.The as-prepared nanoparticles were further modified by γ-(2,3-epoxypropoxy)propytrimethoxysilane(KH-560) to obtain silicone modified Fe3O4 nanoparticles.Also,the water-soluble CdSe/CdS bearing amino have been synthesized using L-cysteine hydrochloride(L-Cys) as stabilizer,At last,CdSe/CdS were linked to Fe3O4 to form luminescent/magnetic bifunctional nanoparticles.The as-prepared composites with a typical diameter of 40nm show 21.287A·m2/kg of saturation magnetic susceptibility.Such material still retains a strong photoluminescence effect of CdSe/CdS quantum dots and also exhibits a typical superparamagnetic behavior of Fe3O4 nanoparticles.
引文
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[5]Ma M,Zhang Y,Yu W,et al.Preparation and character-ization of magnetite nanoparticles coated by amimo silane[J].Colloids and Surfaces A:Physicocheim Eng Aspects2003,212:219-226.
[6]陈庆梅,宗小林.磁性纳米材料及其在癌症诊疗中的应用[J].纳米材料与结构,2009,46(6):340-345.
[7]唐秋莎,张东生,顾宁,等.新型纳米基因载体(PEI/Mn0.5Zn0.5Fe2O4)的制备、表征及体外实验[J].功能材料,2007,38(8):1268-1272.
[8]翁吉芳.水溶性CdTe量子点的生物标记及其生物应用[D].上海:上海交通大学,2006.
[9]Medintz I L,Uyeda H T,Goldman E R,et al.Quantumdot bioconjugates for imaging,labeling and sensing[J].Nat Master,2005,4:435-446.
[10]Bruchez M,Moronne M,Gin P,et al.Semiconductornanocrystals as fluorescent biological lables[J].Sci-ence,1998,281(5385):2013-2016.
[11]Wang Xiuling,Wei Ling,Tao Guanhong,et al.Deter-mination of hydrocortisone in cosmetics by CdSe/CdSquantum dots-based fluoroimmunoassay using magneticFe3O4/Au nanoparticles as solid carriers[J].Chin JChem,2011,29(3):587-593.
[12]Wang Xiuling,Tao Guanhong,Meng Yuanhua.A novelCdSe/CdS quantum dots-based competitive fluoroimmuno-assay for the detection of clenbuterol residue in pig urineusing magnetic core/shell Fe3O4/Au nanoparticles as a sol-id carrier[J].Anal Sci,2009,25(12):1409-1413.
[13]Guo J,Yang W L,Wang C C,et al.Poly(N-isopropylan-crylamide)-coated luminescent magnetic silica micro-spheres:preparation,characterization,and biomedicalapplications[J].Chem Master,2006,18:5554-5562.
[14]贾秋凌,王德平,周萘.载有荧光量子点的纳米磁性微粒的合成方法及其应用前景[J].材料导报,2006,20(2):30-32.
[15]Sun Pan,Zhang Hongyan,Liu Chang,et al.Preparationand characterization of Fe3O4/CdTe magnetic/fluores-cent nanocomposites and their application in immuno-labling and fluorescent imaging of cancer cells[J].Langmuir,2010,26(2):1278-128.
[16]Yang Xu,Karmakar A,Wang Daoyuan,et al.Multi-functional Fe3O4cored magnetic-quantum dot fluores-cent nanocomposites for RF nanohyperthermia of cancercells[J].J Phys Chem C,2010,114(11):5020-5026.
[17]Li Juan,Huang Zhongbing,Wu Dianwu,et al.Prepa-ration and protein detection of Zn ferrite film with mag-netic and photoluminescence properties[J].J PhysChem C,2010,114(3):1586-1592.
[18]李亚茹,张雪莲,潘园园.磁性荧光纳米复合粒子的制备及其表面生物功能化[J].功能材料,2012,43(8):1045-1048.
[19]王秀宇,杨桂琴,张之圣,等.纳米Fe3O4颗粒及磁性液体的制备[J].功能材料与器件学报,2005,11(2):228-232.
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[21]Yang D Z,Chen Q F,Xu S K.Synthesis of CdSe/CdSwith a simple non-TOP-based route[J].Journal of Lu-minescence,2007,126:853-858.
[22]张宝亮,张秋禹,张和鹏,等.负载高密度乙肝检测探针磁珠的制备及性能[J].高等学校化学学报,2011,32(5):1070-1016.