石墨烯/PLGA复合支架对骨髓间充质干细胞增殖和分化能力的影响

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英文篇名：Effects of graphene/PLGA composite scaffolds on proliferation and differentiation of bone marrow mesenchymal stem cells 作者：郑奥 ; 曹玲燕 ; 刘阳 ; 吴建楠 ; 蒋欣泉 英文作者：ZHENG Ao;CAO Lingyan;LIU yang;WU Jiannan;JIANG Xinquan;Department of Prosthodontics,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology & National Clinical Research Center of Stomatology;School of Materials Science and Engineering,East China University of Science and Technology; 关键词：石墨烯 ; 聚乳酸-羟基乙酸共聚物 ; 多孔支架 ; 骨组织工程 英文关键词：graphene;;poly(lactic-co-glycolic acid);;porous scaffold;;bone tissue engineering 中文刊名：KQSW 英文刊名：Oral Biomedicine 机构：上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科;上海市口腔医学重点实验室上海市口腔医学研究所国家口腔疾病临床研究中心;华东理工大学材料与工程学院; 出版日期：2018-06-25 出版单位：口腔生物医学 年：2018 期：v.9 基金：国家重点研发计划(2016YFC1102900);; 国家自然科学基金重点国际(地区)合作研究项目(81620108006);; 上海市科委(国际合作)(15410722700) 语种：中文; 页：KQSW201802001 页数：6 CN：02 ISSN：32-1813/R 分类号：5-9+15

摘要

目的:制备石墨烯(G)/聚乳酸-羟基乙酸共聚物(PLGA)三维多孔复合支架,研究G/PLGA三维多孔复合支架作为骨组织支架材料的可行性。方法:不同质量比的G与PLGA(0,0.5‰,5‰)均匀混合,利用碳酸氢钠致孔制备多孔G/PLGA三维复合支架。通过CCK-8检测、细胞骨架染色、碱性磷酸酶(ALP)活性分析以及RT-PCR实验检测支架材料的细胞毒性及其对大鼠骨髓间充质干细胞(BMSCs)黏附、增殖及分化的影响。结果:扫描电镜结果显示所制备的G/PLGA三维支架材料具有连通的多孔结构,G在支架中均匀分布。CCK-8检测结果显示G/PLGA三维支架无明显细胞毒性。与单纯PLGA支架组相比,G/PLGA三维多孔支架组的BMSCs表达ALP活性有所增加,成骨相关基因表达随G含量升高呈明显上调,其中含有5‰G的复合三维多孔支架的促成骨分化作用最明显。结论:本实验所制备的G/PLGA三维多孔支架具有良好的生物相容性,能够促进BMSCs体外成骨分化,有望作为新型骨组织工程支架材料。
        Objective:This study aimed at fabricating three-dimensional porous graphene(G)/poly(lactic-co-glycolic acid(PLGA) composite scaffolds and establishing the potential for further application of G/PLGA porous scaffolds in bone tissue engineering.Methods:Different concentrations of graphene was mixed with PLGA(G/PLGA,wt.‰:0,0.5‰,5‰) and the porous structure of G/PLGA composite scaffold was formed by using sodium bicarbonate.CCK-8 test,cytoskeleton staining,alkaline phosphatase(ALP)activity test,as well as RT-PCR test were used to detect the cytotoxicity of G/PLGA scaffolds and their effects on the adhesion,proliferation and differentiation of rat bone marrow mesenchymal stem cells(BMSCs).Results:Scanning electron microscopy confirmed the inner connected porous structure of the three-dimensional G/PLGA scaffold as well as the uniform distribution of graphene in the scaffolds.CCK-8 test indicated that G/PLGA porous scaffolds had no obvious cytotoxicity.Compared with BMSCs seeded on PLGA scaffold,the ALP activity of BMSCs seeded on the G/PLGA scaffolds increased and the expression of bone related genes was significantly up-regulated with increase of G concentration.G/PLGA porous scaffold containing 5‰ graphene showed more obvious effects on osteogenic differentiation.Conclusions:The G/PLGA three-dimensional porous scaffold prepared in this research possessed good biocompatibility and could promote osteogenic differentiation of BMSCs in vitro.Thus,it has been expected to be used as a scaffold for bone tissue engineering.

引文

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