干旱胁迫下丁二醇诱导对不同翦股颖品种种子萌发特性的影响
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摘要
【目的】研究PEG-6000模拟干旱胁迫下,诱导剂丁二醇处理对不同品种翦股颖种子萌发的影响.【方法】以翦股颖属的6个品种为供试材料,用不同浓度的两种诱导剂2,3-BD(2,3丁二醇)(0,100,200,300μmol/L)和2R,3R-BD(2R,3R-丁二醇)(0,50,100,150μmol/L)诱导翦股颖种子,采用纸上发芽法(TP)测定在20%PEG-6000模拟干旱胁迫下丁二醇诱导对6个品种翦股颖种子萌发和生长的影响.【结果】干旱胁迫条件下,不同浓度的两种丁二醇诱导使种子萌发期一些指标发生变化.对发芽势影响不太明显,对发芽率、发芽指数、活力指数、抗旱萌发指数和胁迫指数均有促进作用,且2,3-BD处理相比2R,3R-BD处理效果更显著,其中浓度为200μmol/L的2,3-BD促进作用最明显.【结论】丁二醇诱导能使种子萌发期抗旱能力得到一定的提升,总体来看,200μmol/L的2,3-BD效果最佳,但品种间存在差异.
【Objective】The effect of butanediol treatment on seed germination of different bentgrass cultivars was studied under drought stress simulated by PEG-6000.【Method】Under the simulated drought stress with 20% PEG-6000,the TP method was used to determine the effect of different concentrations of two inducers including 2,3-BD(0,100,200,300μmol/L)and 2 R,3 R-BD(0,50,100,150μmol/L)on the seed germination and growth of six bentgrass cultivars.【Result】Under the drought stress,the two kinds of butanediol with different concentrations induced some changes in seed germination indexes,except for the germination potential,the germination rate,germination index,vigor index,drought-resistant germination index and stress index were all promoted,and the effect of 2,3-BD treatment was more obvious than2 R,3 R-BD,and the concentration of 200μmol/L 2,3-BD showed the most obvious promoting effect.【Conclusion】The butanediol induction improved drought resistance in seed germination of bentgrass and 200μmol/L 2,3-BD had the best promoting effect and there was difference among cultivars.
【Objective】The effect of butanediol treatment on seed germination of different bentgrass cultivars was studied under drought stress simulated by PEG-6000.【Method】Under the simulated drought stress with 20% PEG-6000,the TP method was used to determine the effect of different concentrations of two inducers including 2,3-BD(0,100,200,300μmol/L)and 2 R,3 R-BD(0,50,100,150μmol/L)on the seed germination and growth of six bentgrass cultivars.【Result】Under the drought stress,the two kinds of butanediol with different concentrations induced some changes in seed germination indexes,except for the germination potential,the germination rate,germination index,vigor index,drought-resistant germination index and stress index were all promoted,and the effect of 2,3-BD treatment was more obvious than2 R,3 R-BD,and the concentration of 200μmol/L 2,3-BD showed the most obvious promoting effect.【Conclusion】The butanediol induction improved drought resistance in seed germination of bentgrass and 200μmol/L 2,3-BD had the best promoting effect and there was difference among cultivars.
引文
[1]周久,刘建秀,陈树元.草坪草抗旱性研究概述[J].草业科学,2002,19(5):61-68.
[2]王东芳,张飞,周梅,等.农业干旱风险研究进展与发展趋势[J].安徽农业科学,2014,42(17):5455-5458.
[3]王艳树,李凤山,张玉霞,等.PEG胁迫对蓖麻种子吸胀萌发的影响[J].内蒙古民族大学学报(自然科学版),2007,22(3):302-306.
[4]孙吉雄.草坪学[M].北京:中国农业出版社,1995.
[5]BONOS S A,CASLER M D,MEYER W A.Plant responses and characteristics associated with dollar spot resistance in creeping bentgrass[J].Crop Science,2004,44(5):1763-1769.
[6]安永平,强爱玲,张媛媛,等.渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究[J].植物遗传资源学报,2006,7(4):421-426.
[7]王颖,穆春生,王靖,等.松嫩草地主要豆科牧草种子萌发期耐旱性差异研究[J].中国草地学报,2006,28(1):7-12.
[8]傅华龙,何天久,吴巧玉.植物生长调节剂的研究与应用[J].生物加工过程,2008,6(7):7-12.
[9]金一锋,陈阳,董亚楠,等.外源水杨酸对草地早熟禾种子萌发及幼苗抗旱性的影响[J].种子,2015,34(4):25-28.
[10]吕山花,樊颖伦.壳聚糖对玉米种子萌发及耐旱性的影响[J].玉米科学,2011,19(1):92-94
[11]谢腾龙,孟瑶,顾万荣,等.DCPTA诱导寒地春玉米种子萌发及幼苗抗旱理化特征的研究[J].中国植保导刊,2015,35(6):11-19
[12]王竞红,多多.多效唑对6种草坪草苗期抗旱性影响的研究[J].草业学报,2014,23(6):253-258.
[13]王学智,曹敏建,蒋文春.抗旱剂处理对玉米种子的萌发及苗期抗旱力的影响[J].玉米科学,2005(2):82-84,89.
[14]曹丽,回振龙,魏小红,等.SNP对PEG模拟干旱胁迫下早熟禾种子萌发及幼苗抗性的影响[J].甘肃农业大学学报,2013,48(5):100-106,113.
[15]马玉花,刘品高,高英,等.干旱胁迫下‘肋果沙棘'渗透调节物及保护酶活性变化的研究[J].甘肃农业大学学报,2013,48(4):100-104.
[16]陈志飞.外源植物激素对干旱胁迫下高羊茅生长的缓解效应研究[D].杨凌:西北农林科技大学,2016.
[17]崛田康司,蒋卫平.5—氨基乙酰丙酸对两种草坪生长的影响[J].青海草业,2001(4):46-47.
[18]RYU C,FARAG M A,HU C,et al.Bacterial volatiles induce systemic resistance in Arabidopsis[J].Plant Physiology,2004,134(3):1017-1026.
[19]HAN S H,LEE S J,MOON J H,et al.GacS-dependent production of 2R,3R-butanediol by Pseudomonas chlororaphis O6is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv.tabaci in tobacco[J].Molecular Plant-Microbe Interactions,2006,19(8):924-930.
[20]梁国岭,周青平,颜红波.聚乙二醇对羊茅属4种植物种子萌发特性的影响研[J].草业科学,2007,24(6):50-54.
[21]MEICHEL B E,KAUFMANN M R.The osmotic potential of polyethylene glycol-6000[J].plant physiology,1973,51(5):914-916
[22]汪建军,麻安卫,汪治刚,等.不同温度和PEG处理对中华羊茅种子萌发的影响[J].草业学报,2016,25(4):73-80.
[23]王赞,李源,吴欣明,等.PEG渗透胁迫下鸭茅种子萌发特性及抗旱性鉴定[J].中国草地学报,2008,30(1):50-55.
[24]魏永胜,梁宗锁,山仑,等.利用隶属函数值法评价苜蓿抗旱性[J].草业科学,2005,22(6):33-36.
[25]张健,池宝亮,黄学芳,等.玉米萌芽期水分胁迫的抗旱性分析[J].山西农业科学,2007,35(2):34-35
[26]徐明慧,关义新,马兴林,等.玉米芽苗期抗旱性研究进展综述[J].玉米科学,2002,10(4):35-38
[27]岑画梦,彭玲莉,杨雪,等.Cd2+对狗牙根、假俭草种子萌发及幼苗生长的影响[J].草业学报,2015,24(5):100-107.
[28]BOUSLAMA M,SCHAPAUGH W T Jr.Stress tolerance in soybeans.I.Evaluation of three screening techniques for heat and drought tolerance[J].Crop Science,1984,24(5):933-937
[29]黄义春,李建民,段留生,等.甜菜碱对玉米幼苗抗旱性的诱导效应[J].玉米科学,2011(1):95-100.
[30]刘芳芳,高洋,赵春旭,等.干旱胁迫下多效唑浸种对多年生黑麦草种子萌发的影响[J].草业科学,2011,28(12):2143-2149.
[2]王东芳,张飞,周梅,等.农业干旱风险研究进展与发展趋势[J].安徽农业科学,2014,42(17):5455-5458.
[3]王艳树,李凤山,张玉霞,等.PEG胁迫对蓖麻种子吸胀萌发的影响[J].内蒙古民族大学学报(自然科学版),2007,22(3):302-306.
[4]孙吉雄.草坪学[M].北京:中国农业出版社,1995.
[5]BONOS S A,CASLER M D,MEYER W A.Plant responses and characteristics associated with dollar spot resistance in creeping bentgrass[J].Crop Science,2004,44(5):1763-1769.
[6]安永平,强爱玲,张媛媛,等.渗透胁迫下水稻种子萌发特性及抗旱性鉴定指标研究[J].植物遗传资源学报,2006,7(4):421-426.
[7]王颖,穆春生,王靖,等.松嫩草地主要豆科牧草种子萌发期耐旱性差异研究[J].中国草地学报,2006,28(1):7-12.
[8]傅华龙,何天久,吴巧玉.植物生长调节剂的研究与应用[J].生物加工过程,2008,6(7):7-12.
[9]金一锋,陈阳,董亚楠,等.外源水杨酸对草地早熟禾种子萌发及幼苗抗旱性的影响[J].种子,2015,34(4):25-28.
[10]吕山花,樊颖伦.壳聚糖对玉米种子萌发及耐旱性的影响[J].玉米科学,2011,19(1):92-94
[11]谢腾龙,孟瑶,顾万荣,等.DCPTA诱导寒地春玉米种子萌发及幼苗抗旱理化特征的研究[J].中国植保导刊,2015,35(6):11-19
[12]王竞红,多多.多效唑对6种草坪草苗期抗旱性影响的研究[J].草业学报,2014,23(6):253-258.
[13]王学智,曹敏建,蒋文春.抗旱剂处理对玉米种子的萌发及苗期抗旱力的影响[J].玉米科学,2005(2):82-84,89.
[14]曹丽,回振龙,魏小红,等.SNP对PEG模拟干旱胁迫下早熟禾种子萌发及幼苗抗性的影响[J].甘肃农业大学学报,2013,48(5):100-106,113.
[15]马玉花,刘品高,高英,等.干旱胁迫下‘肋果沙棘'渗透调节物及保护酶活性变化的研究[J].甘肃农业大学学报,2013,48(4):100-104.
[16]陈志飞.外源植物激素对干旱胁迫下高羊茅生长的缓解效应研究[D].杨凌:西北农林科技大学,2016.
[17]崛田康司,蒋卫平.5—氨基乙酰丙酸对两种草坪生长的影响[J].青海草业,2001(4):46-47.
[18]RYU C,FARAG M A,HU C,et al.Bacterial volatiles induce systemic resistance in Arabidopsis[J].Plant Physiology,2004,134(3):1017-1026.
[19]HAN S H,LEE S J,MOON J H,et al.GacS-dependent production of 2R,3R-butanediol by Pseudomonas chlororaphis O6is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv.tabaci in tobacco[J].Molecular Plant-Microbe Interactions,2006,19(8):924-930.
[20]梁国岭,周青平,颜红波.聚乙二醇对羊茅属4种植物种子萌发特性的影响研[J].草业科学,2007,24(6):50-54.
[21]MEICHEL B E,KAUFMANN M R.The osmotic potential of polyethylene glycol-6000[J].plant physiology,1973,51(5):914-916
[22]汪建军,麻安卫,汪治刚,等.不同温度和PEG处理对中华羊茅种子萌发的影响[J].草业学报,2016,25(4):73-80.
[23]王赞,李源,吴欣明,等.PEG渗透胁迫下鸭茅种子萌发特性及抗旱性鉴定[J].中国草地学报,2008,30(1):50-55.
[24]魏永胜,梁宗锁,山仑,等.利用隶属函数值法评价苜蓿抗旱性[J].草业科学,2005,22(6):33-36.
[25]张健,池宝亮,黄学芳,等.玉米萌芽期水分胁迫的抗旱性分析[J].山西农业科学,2007,35(2):34-35
[26]徐明慧,关义新,马兴林,等.玉米芽苗期抗旱性研究进展综述[J].玉米科学,2002,10(4):35-38
[27]岑画梦,彭玲莉,杨雪,等.Cd2+对狗牙根、假俭草种子萌发及幼苗生长的影响[J].草业学报,2015,24(5):100-107.
[28]BOUSLAMA M,SCHAPAUGH W T Jr.Stress tolerance in soybeans.I.Evaluation of three screening techniques for heat and drought tolerance[J].Crop Science,1984,24(5):933-937
[29]黄义春,李建民,段留生,等.甜菜碱对玉米幼苗抗旱性的诱导效应[J].玉米科学,2011(1):95-100.
[30]刘芳芳,高洋,赵春旭,等.干旱胁迫下多效唑浸种对多年生黑麦草种子萌发的影响[J].草业科学,2011,28(12):2143-2149.
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