太湖竺山湾春季浮游细菌群落结构及影响因素
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摘要
为研究太湖竺山湾春季浮游细菌群落结构,利用高通量测序对竺山湾4个采样点(雅浦港、沙塘港、竺山湖南和椒山)浮游细菌的16S rRNA基因进行序列测定.结果表明:测序文库的覆盖率很高,测序结果完全可以代表样本的真实情况;椒山的物种丰富度最高,但物种分布均匀度较低;竺山湾主要优势菌门为蓝藻门(Cyanobacteria)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),蓝藻门平均丰度高达64.73%,正处于水体复苏阶段,已有蓝藻水华暴发态势.属水平优势细菌主要有鱼腥藻属(Anabaena)、hgc I_clade、CL500-29_marine_group、微囊藻属(Microcystis)、聚球藻属(Synechococcus)和分枝杆菌属(Mycobacterium);运用冗余分析(RDA)探讨浮游细菌与环境因子的关系,结果表明,水温、Chl-a、NH+4-N、DO和PO3-4-P是影响浮游细菌群落的主要环境因子,其中,DO能显著影响微囊藻属,营养盐和水温对其也有一定程度影响.
In order to investigate the characteristics of bacterioplankton in the spring in Zhushan Bay,Lake Taihu,the 16 S rRNA gene of the bacterioplankton at four sampling sites in Zhushan Bay was sequenced by high-throughput sequencing using water samples collected from Yapugang,Shatanggang,Zhushanhunan,and Jiaoshan. The results showed that the coverage of the sequencing library was very high and could accurately represent the bacterioplankton community in the samples. The species richness of Jiaoshan was the highest,but the species evenness was lower. Cyanobacteria,Actinobacteria,Proteobacteria,and Bacteroidetes were the dominant phylum in Zhushan Bay. The average abundance of Cyanobacteria was as high as 64. 73%, which indicated an outbreak of cyanobacteria bloom in the water. At the genus level,Anabaena,hgc I_clade,CL500-29_marine_group,Microcystis,Synechococcus,and Mycobacterium were predominant. The results of redundancy analysis(RDA) for the relationship between bacterioplankton and environmental factors showed that water temperature,chlorophyll a(Chl-a),ammonia nitrogen(NH+4-N),dissolved oxygen(DO),and phosphate(PO3-4-P) were the main environmental factors affecting the bacterioplankton community. Dissolved oxygen could significantly affect Microcystis; nutrient and water temperature also had an effect.
In order to investigate the characteristics of bacterioplankton in the spring in Zhushan Bay,Lake Taihu,the 16 S rRNA gene of the bacterioplankton at four sampling sites in Zhushan Bay was sequenced by high-throughput sequencing using water samples collected from Yapugang,Shatanggang,Zhushanhunan,and Jiaoshan. The results showed that the coverage of the sequencing library was very high and could accurately represent the bacterioplankton community in the samples. The species richness of Jiaoshan was the highest,but the species evenness was lower. Cyanobacteria,Actinobacteria,Proteobacteria,and Bacteroidetes were the dominant phylum in Zhushan Bay. The average abundance of Cyanobacteria was as high as 64. 73%, which indicated an outbreak of cyanobacteria bloom in the water. At the genus level,Anabaena,hgc I_clade,CL500-29_marine_group,Microcystis,Synechococcus,and Mycobacterium were predominant. The results of redundancy analysis(RDA) for the relationship between bacterioplankton and environmental factors showed that water temperature,chlorophyll a(Chl-a),ammonia nitrogen(NH+4-N),dissolved oxygen(DO),and phosphate(PO3-4-P) were the main environmental factors affecting the bacterioplankton community. Dissolved oxygen could significantly affect Microcystis; nutrient and water temperature also had an effect.
引文
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[29]Tang X M,Gao G,Qin B Q,et al.Characterization of bacterial communities associated with organic aggregates in a large,shallow,eutrophic freshwater lake(Lake Taihu,China)[J].Microbial Ecology,2009,58(2):307-322.
[30]Tang X M,Chao J Y,Gong Y,et al.Spatiotemporal dynamics of bacterial community composition in large shallow eutrophic Lake Taihu:high overlap between free-living and particle-attached assemblages[J].Limnology and Oceanography,2017,62(4):1366-1382.
[31]Fan L M,Song C,Meng S L,et al.Spatial distribution of planktonic bacterial and archaeal communities in the upper section of the tidal reach in Yangtze River[J].Scientific Reports,2016,6:39147.
[32]Liu Y Q,Yao T D,Jiao N Z,et al.Seasonal dynamics of the bacterial community in Lake Namco,the largest Tibetan Lake[J].Geomicrobiology Journal,2013,30(1):17-28.
[33]Shi L M,Cai Y F,Wang X Y,et al.Community structure of bacteria associated with Microcystis colonies from cyanobacterial blooms[J].Journal of Freshwater Ecology,2010,25(2):193-203.
[34]李建柱,侯杰,张鹏飞,等.空心菜浮床对鱼塘水质和微生物多样性的影响[J].中国环境科学,2016,36(10):3071-3080.Li J Z,Hou J,Zhang P F,et al.Influence on water quality and microbial diversity in fish pond by Ipomoea aquatica floating-bed[J].China Environmental Science,2016,36(10):3071-3080.
[35]Sand-Jensen K,Borum J.Interactions among phytoplankton,periphyton,and macrophytes in temperate freshwaters and estuaries[J].Aquatic Botany,1991,41(1-3):137-175.
[36]Su X M,Steinman A D,Xue Q J,et al.Temporal patterns of phyto-and bacterioplankton and their relationships with environmental factors in Lake Taihu,China[J].Chemosphere,2017,184:299-308.
[37]Wei G S,Li J,Wang N X,et al.Spatial abundance and diversity of bacterioplankton in a typical stream-forming ecosystem,Huangqian Reservoir,China[J].Journal of Microbiology and Biotechnology,2014,24(10):1308-1318.
[38]Hu M M,Li Y H,Wang Y C,et al.Phytoplankton and bacterioplankton abundances and community dynamics in Lake Erhai[J].Water Science&Technology,2013,68(2):348-56.
[39]Bouhaddada R,Nélieu S,Nasri H,et al.High diversity of microcystins in a Microcystis,bloom from an Algerian lake[J].Environmental Pollution,2016,216:836-844.
[40]Liu X,Lu X H,Chen Y W.The effects of temperature and nutrient ratios on Microcystis,blooms in Lake Taihu,China:an11-year investigation[J].Harmful Algae,2011,10(3):337-343.
[2]Qin B Q,Zhu G W,Gao G,et al.A drinking water crisis in Lake Taihu,China:linkage to climatic variability and lake management[J].Environmental Management,2010,45(1):105-112.
[3]Dziallas C,Grossart H P.Increasing oxygen radicals and water temperature select for toxic Microcystis sp[J].PLo S One,2011,6(9):e25569.
[4]Paerl H W,Huisman J.Blooms like it hot[J].Science,2008,320(5872):57-58.
[5]宜兴日报.太湖蓝藻打捞量是去年同期的5.5倍[EB/OL].http://www.yixing.gov.cn/zxzx/show-13593314.html,2017-05-16.
[6]Li J F,Zhang J Y,Liu L Y,et al.Annual periodicity in planktonic bacterial and archaeal community composition of eutrophic Lake Taihu[J].Scientific Reports,2015,5:15488.
[7]王雯雯,姜霞,王书航,等.太湖竺山湾污染底泥环保疏浚深度的推算[J].中国环境科学,2011,31(6):1013-1018.Wang W W,Jiang X,Wang S H,et al.Calculation of environmental dredging depth of contaminated sediments in Zhushan Bay of Taihu Lake[J].China Environmental Science,2011,31(6):1013-1018.
[8]向燕,吴宇澄,刘国锋,等.太湖竺山湾沉积物中氨氧化原核生物的垂直分布与多样性[J].生态学报,2010,30(6):1423-1430.Xiang Y,Wu Y C,Liu G F,et al.Vertical distribution and diversity of ammonia-oxidizing prokaryotes in a sediment core from Zhushan Bay,Lake Taihu[J].Acta Ecologica Sinica,2010,30(6):1423-1430.
[9]Niu Y,Shen H,Chen J,et al.Phytoplankton community succession shaping bacterioplankton community composition in Lake Taihu,China[J].Water Research,2011,45(14):4169-4182.
[10]Tian C,Tan J,Wu X,et al.Spatiotemporal transition of bacterioplankton diversity in a large shallow hypertrophic freshwater lake,as determined by denaturing gradient gel electrophoresis[J].Journal of Plankton Research,2009,31(8):885-897.
[11]Li H B,Xing P,Wu Q L.Characterization of the bacterial community composition in a hypoxic zone induced by Microcystis blooms in Lake Taihu,China[J].FEMS Microbiology Ecology,2012,79(3):773-784.
[12]顾婷婷,孔繁翔,谭啸,等.越冬和复苏时期太湖水体蓝藻群落结构的时空变化[J].生态学报,2011,31(1):21-30.Gu T T,Kong F X,Tan X,et al.Investigation on spatiotemporal pattern of cyanobacterial community structure by TRFLP during overwinter and recruitment period in Taihu Lake[J].Acta Ecologica Sinica,2011,31(1):21-30.
[13]Czechowski P,Clarke L J,Breen J,et al.Antarctic eukaryotic soil diversity of the Prince Charles Mountains revealed by highthroughput sequencing[J].Soil Biology and Biochemistry,2016,95:112-121.
[14]魏佳明,崔丽娟,李伟,等.表流湿地细菌群落结构特征[J].环境科学,2016,37(11):4357-4365.Wei J M,Cui L J,Li W,et al.Characteristics of bacterial communities in surface-flow constructed wetlands[J].Environmental Science,2016,37(11):4357-4365.
[15]刘洋,黄懿梅,曾全超.黄土高原不同植被类型下土壤细菌群落特征研究[J].环境科学,2016,37(10):3931-3938.Liu Y,Huang Y M,Zeng Q C.Soil bacterial communities under different vegetation types in the Loess Plateau[J].Environmental Science,2016,37(10):3931-3938.
[16]Chao Y Q,Mao Y P,Wang Z P,et al.Diversity and functions of bacterial community in drinking water biofilms revealed by highthroughput sequencing[J].Scientific Reports,2015,5:10044.
[17]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002.
[18]薛银刚,蒋聪,耿金菊,等.基于q PCR和16S r DNA高通量测序研究蓝藻暴发期间太湖竺山湾水体浮游细菌群落[J].环境监控与预警,2017,9(3):19-23.Xue Y G,Jiang C,Geng J J,et al.Profiles of bacterioplankton based on q PCR and 16S r DNA High-throughput Sequencing during a heavy cyanobacterial bloom in Zhushan bay,Taihu Lake[J].Environmental Monitoring and Forewarning,2017,9(3):19-23.
[19]De Santis T Z,Hugenholtz P,Larsen N,et al.Greengenes,a chimera-checked 16S rRNA gene database and workbench compatible with ARB[J].Applied and Environmental Microbiology,2006,72(7):5069-5072.
[20]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[21]Ter Braak C J F,Smilauer P.CANOCO reference manual and Cano Draw for Windows user's guide:software for canonical community ordination(version 4.5)[M].Ithaca,New York:Microcomputer Power,2002.
[22]荆红卫,华蕾,孙成华,等.北京城市湖泊富营养化评价与分析[J].湖泊科学,2008,20(3):357-363.Jing H W,Hua L,Sun C H,et al.Analysis on urban lakes'eutrophication status in Beijing[J],Journal of Lake Sciences,2008,20(3):357-363.
[23]Amato K R,Yeoman C J,Kent A,et al.Habitat degradation impacts black howler monkey(Alouatta pigra)gastrointestinal microbiomes[J].The ISME Journal,2013,7(7):1344-1353.
[24]Zhou J Z,Jiang Y H,Deng Y,et al.Random sampling process leads to overestimation ofβ-diversity of microbial communities[J].m Bio,2013,4(3):e00324-13.
[25]任丽娟,何聃,邢鹏,等.湖泊水体细菌多样性及其生态功能研究进展[J].生物多样性,2013,21(4):421-432.Ren L J,He D,Xing P,et al.Bacterial diversity and ecological function in lake water bodies[J].Biodiversity Science,2013,21(4):421-432.
[26]Paerl H W,Hall N S,Calandrino E S.Controlling harmful cyanobacterial blooms in a world experiencing anthropogenic and climatic-induced change[J].Science of the Total Environment,2011,409(10):1739-1745.
[27]O'Neil J M,Davis T W,Burford M A,et al.The rise of harmful cyanobacteria blooms:the potential roles of eutrophication and climate change[J].Harmful Algae,2012,14:313-334.
[28]孔繁翔,马荣华,高俊峰,等.太湖蓝藻水华的预防、预测和预警的理论与实践[J].湖泊科学,2009,21(3):314-328.Kong F X,Ma R H,Gao J F,et al.The theory and practice of prevention,forecast and warning on cyanobacteria bloom in Lake Taihu[J].Journal of Lake Sciences,2009,21(3):314-328.
[29]Tang X M,Gao G,Qin B Q,et al.Characterization of bacterial communities associated with organic aggregates in a large,shallow,eutrophic freshwater lake(Lake Taihu,China)[J].Microbial Ecology,2009,58(2):307-322.
[30]Tang X M,Chao J Y,Gong Y,et al.Spatiotemporal dynamics of bacterial community composition in large shallow eutrophic Lake Taihu:high overlap between free-living and particle-attached assemblages[J].Limnology and Oceanography,2017,62(4):1366-1382.
[31]Fan L M,Song C,Meng S L,et al.Spatial distribution of planktonic bacterial and archaeal communities in the upper section of the tidal reach in Yangtze River[J].Scientific Reports,2016,6:39147.
[32]Liu Y Q,Yao T D,Jiao N Z,et al.Seasonal dynamics of the bacterial community in Lake Namco,the largest Tibetan Lake[J].Geomicrobiology Journal,2013,30(1):17-28.
[33]Shi L M,Cai Y F,Wang X Y,et al.Community structure of bacteria associated with Microcystis colonies from cyanobacterial blooms[J].Journal of Freshwater Ecology,2010,25(2):193-203.
[34]李建柱,侯杰,张鹏飞,等.空心菜浮床对鱼塘水质和微生物多样性的影响[J].中国环境科学,2016,36(10):3071-3080.Li J Z,Hou J,Zhang P F,et al.Influence on water quality and microbial diversity in fish pond by Ipomoea aquatica floating-bed[J].China Environmental Science,2016,36(10):3071-3080.
[35]Sand-Jensen K,Borum J.Interactions among phytoplankton,periphyton,and macrophytes in temperate freshwaters and estuaries[J].Aquatic Botany,1991,41(1-3):137-175.
[36]Su X M,Steinman A D,Xue Q J,et al.Temporal patterns of phyto-and bacterioplankton and their relationships with environmental factors in Lake Taihu,China[J].Chemosphere,2017,184:299-308.
[37]Wei G S,Li J,Wang N X,et al.Spatial abundance and diversity of bacterioplankton in a typical stream-forming ecosystem,Huangqian Reservoir,China[J].Journal of Microbiology and Biotechnology,2014,24(10):1308-1318.
[38]Hu M M,Li Y H,Wang Y C,et al.Phytoplankton and bacterioplankton abundances and community dynamics in Lake Erhai[J].Water Science&Technology,2013,68(2):348-56.
[39]Bouhaddada R,Nélieu S,Nasri H,et al.High diversity of microcystins in a Microcystis,bloom from an Algerian lake[J].Environmental Pollution,2016,216:836-844.
[40]Liu X,Lu X H,Chen Y W.The effects of temperature and nutrient ratios on Microcystis,blooms in Lake Taihu,China:an11-year investigation[J].Harmful Algae,2011,10(3):337-343.
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