太湖不同湖区冬季沉积物细菌群落多样性
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摘要
为了探究太湖不同营养水平湖区冬季沉积物细菌群落多样性,利用高通量测序对太湖水草区、湖心区和河口区共9个采样点表层沉积物细菌的16S r RNA基因进行序列测定.结果发现:不同湖区的物种丰富度和均匀度为水草区>湖心区>河口区;不同湖区优势细菌群落组成存在差异.其中,硝化螺旋菌门(Nitrospirae)和酸杆菌门(Acidobacteria)2类门水平优势细菌以及厌氧绳菌纲(Anaerolineae)和硝化螺菌纲(Nitrospira)2类纲水平优势细菌在不同湖区之间差异显著.冬季太湖沉积物细菌优势类群为绿弯菌门(Chloroflexi)、变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)和硝化螺旋菌门(Nitrospirae);在属分类水平上,主要优势类群为unidentified_Chloroplast和微囊藻属(Microcystis).Cyanobacteria和Microcystis在所有采样点均有检出,平均丰度均以湖心区最高.对沉积物细菌群落与环境因子的关系进行冗余分析,结果表明:营养盐水平、水温和pH值均能影响沉积物细菌群落结构,NO_3~--N和水温是Microcystis的主要影响因子.
To explore the diversity of bacterial communities in the sediment of different lake zones with varied nutrition levels in Lake Taihu in winter, the 16 S r RNA gene of surficial sedimentary bacteria of 9sampling sites, including the estuary area, the lake center and the macrophyte-dominated area, was sequenced using high-throughput sequencing approach. The results showed that the species richness and evenness was the highest in the macrophyte-dominated area, moderated in the lake center, while lowest in the estuary area. The composition of bacterial communities varied among lake zones: the phyla of Nitrospirae and Acidobacteria, and the classes of Anaerolineae and Nitrospira, specifically showed significant difference among lake zones with different nutrition levels. At the phyla level, Proteobacteria, Chloroflexi, Cyanobacteria and Nitrospirae were dominant. unidentified_Chloroplast and Microcystis were dominant bacteria at the genus level in the surficial sediments of Lake Taihu in winter. Cyanobacteria and Microcystis were detected ubiquitously at all sampling sites with the highest average abundance appearing in the center of Lake. The redundancy analysis(RDA) for the relationship between bacterial communities and environmental factors revealed that the nutrition level, water temperature and p H could affect the bacterial community structure in sediment. The level of nitrate nitrogen(NO_3~--N) and water temperature were the major environmental factors affecting the genus of Microcystis.
To explore the diversity of bacterial communities in the sediment of different lake zones with varied nutrition levels in Lake Taihu in winter, the 16 S r RNA gene of surficial sedimentary bacteria of 9sampling sites, including the estuary area, the lake center and the macrophyte-dominated area, was sequenced using high-throughput sequencing approach. The results showed that the species richness and evenness was the highest in the macrophyte-dominated area, moderated in the lake center, while lowest in the estuary area. The composition of bacterial communities varied among lake zones: the phyla of Nitrospirae and Acidobacteria, and the classes of Anaerolineae and Nitrospira, specifically showed significant difference among lake zones with different nutrition levels. At the phyla level, Proteobacteria, Chloroflexi, Cyanobacteria and Nitrospirae were dominant. unidentified_Chloroplast and Microcystis were dominant bacteria at the genus level in the surficial sediments of Lake Taihu in winter. Cyanobacteria and Microcystis were detected ubiquitously at all sampling sites with the highest average abundance appearing in the center of Lake. The redundancy analysis(RDA) for the relationship between bacterial communities and environmental factors revealed that the nutrition level, water temperature and p H could affect the bacterial community structure in sediment. The level of nitrate nitrogen(NO_3~--N) and water temperature were the major environmental factors affecting the genus of Microcystis.
引文
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[31]Paerl H W,Huisman J.Blooms like it hot[J].Science,2008,320(5872):57-58.
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[35]Yu D,Yang Y,Zhen W,et al.Spatiotemporal variation of planktonic and sediment bacterial assemblages in two plateau freshwater lakes at different trophic status[J].Applied Microbiology and Biotechnology,2016,100(9):4161-4175.
[36]GB/3838-2002地表水环境质量标准[S].
[37]Winters A D,Marsh T L,Brenden T O,et al.Molecular characterization of bacterial communities associated with sediments in the Laurentian Great Lakes[J].Journal of Great Lakes Research,2014,40(3):640-645.
[38]Jing W,Yi L,Wang P F,et al.Response of bacterial community compositions to different sources of pollutants in sediments of a tributary of Taihu Lake,China[J].Environmental Science and Pollution Research International,2016,23(14):13886-13894.
[39]Liu X,Lu X H,Chen Y W.The effects of temperature and nutrient ratios on Microcystis blooms in Lake Taihu,China:an11-year investigation[J].Harmful Algae,2011,10(3):337-343.
[40]Wang X Y,Sun M J,Wang J M,et al.Microcystis genotype succession and related environmental factors in Lake Taihu during cyanobacterial blooms[J].Microbial Ecology,2012,64(4):986-999.
[41]Otten T G,Xu H,Qin B Q,et al.Spatiotemporal patterns and ecophysiology of toxigenic microcystis blooms in Lake Taihu,China:implications for water quality management[J].Environmental Science and Technology,2016,46(6):3480-3488.
[2]Ye W J,Liu X L,Lin S Q,et al.The vertical distribution of bacterial and archaeal communities in the water and sediment of Lake Taihu[J].Fems Microbiology Ecology,2009,70(2):263-276.
[3]Yang Z,Zhang M,Shi X L,et al.Nutrient reduction magnifies the impact of extreme weather on cyanobacterial bloom formation in large shallow Lake Taihu(China)[J].Water Research,2016,103:302-310.
[4]郑艳玲,侯立军,陆敏,等.崇明东滩夏冬季表层沉积物细菌多样性研究[J].中国环境科学,2012,32(2):300-310.
[5]顾婷婷,孔繁翔,谭啸,等.越冬和复苏时期太湖水体蓝藻群落结构的时空变化[J].生态学报,2011,31(1):21-30.
[6]Tang X M,Gao G A,Chao J Y,et al.Dynamics of organicaggregate-associated bacterial communities and related environmental factors in Lake Taihu,a large eutrophic shallow lake in China[J].Limnology and Oceanography,2010,55(2):469-480.
[7]Shi L M,Cai Y F,Kong F X,et al.Specific association between bacteria and buoyant Microcystis,colonies compared with other bulk bacterial communities in the eutrophic Lake Taihu,China[J].Environmental Microbiology Reports,2012,4(6):669-678.
[8]陈兆进,陈海燕,李玉英,等.南水北调中线干渠(河南段)浮游细菌群落组成及影响因素[J].中国环境科学,2017,37(4):1505-1513.
[9]Chao Y Q,Mao Y P,Wang Z P,et al.Diversity and functions of bacterial community in drinking water biofilms revealed by high-throughput sequencing[J].Scientific Reports,2015,5:10044.
[10]Sun Y J,Wang T Y,Peng X W,et al.Bacterial community compositions in sediment polluted by perfluoroalkyl acids(PFAAs)using Illumina high-throughput sequencing[J].Environmental Science and Pollution Research,2016,23(11):10556-10565.
[11]刘洋,曾全超,黄懿梅.基于454高通量测序的黄土高原不同乔木林土壤细菌群落特征[J].中国环境科学,2016,36(11):3487-3494.
[12]白洁,李海艳,张健,等.黄海西北部沉积物中细菌群落16S r DNA多样性解析[J].中国环境科学,2009,29(12):1277-1284.
[13]Shao K Q,Gao G,Qin B Q,et al.Structural changes of the sediment bacterial community in Meiliang Bay of Lake Taihu,China,during typhoon Morakot,2009[J].Journal of Basic Microbiology,2012,52(1):99-103.
[14]Tang X M,Li L L,Shao K Q,et al.Pyrosequencing analysis of free-living and attached bacterial communities in Meiliang Bay,Lake Taihu,a large eutrophic shallow lake in China[J].Canadian Journal of Microbiology,2014,61(1):22-31.
[15]国家环境保护总局.水和废水监测分析方法-第4版[M].北京:中国环境科学出版社,2002:239-284.
[16]Desantis T Z,Hugenholtz P,Larsen N,et al.Greengenes:a chimera-checked 16S r RNA gene database and workbench compatible in ARB[J].Applied&Environmental Microbiology,2006,72(7):5069-5072.
[17]Caporaso J G,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[18]Ter Braak C J F,Smilauer P.CANOCO reference manual and Cano Draw for Windows user's guide:software for canonical community ordination(version 4.5)[M].Ithaca,New York,Microcomputer Power,2002.
[19]Huse S H,Welch D M,Morrison H G,et al.Ironing out the wrinkles in the rare biosphere through improved OTU clustering[J].Environmental Microbiology,2010,12(7):1889-1898.
[20]陈兆进,丁传雨,朱静亚,等.丹江口水库枯水期浮游细菌群落组成及影响因素研究[J].中国环境科学,2017,37(1):336-344.
[21]Zhou J,Jiang Y H,Deng Y,et al.Random sampling process leads to overestimation ofβ-diversity of microbial communities[J].Mbio,2013,4(3):e00324-13.
[22]逄勇,颜润润,余钟波,等.风浪作用下的底泥悬浮沉降及内源释放量研究[J].环境科学,2008,29(9):2456-2464.
[23]Strecker A L,Arnott S E,Yan N D,et al.Variation in the response of crustacean zooplankton species richness and composition to the invasive predator Bythotrephes longimanus[J].Canadian Journal of Fisheries and Aquatic Sciences,2006,63(9):2126-2136.
[24]Sand-Jensen K,Borum J.Interactions among phytoplankton,periphyton,and macrophytes in temperate freshwaters and estuaries[J].Aquatic Botany,1991,41(1-3):137-175.
[25]Liu F H,Lin G H,Gao G,et al.Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake,Lake Taihu,as revealed by denaturing gradient gel electrophoresis[J].Journal of Applied Microbiology,2009,106(3):1022-1032.
[26]Nam Y D,Sung Y,Chang H W,et al.Characterization of the depth-related changes in the microbial communities in Lake Hovsgol sediment by 16S r RNA gene-based approaches[J].Journal of Microbiology,2008,46(2):125-136.
[27]Madsen E L.Microorganisms and their roles in fundamental biogeochemical cycles[J].Current Opinion in Biotechnology,2011,22(3):456-464.
[28]Shao K Q,Gao G,Qin B Q,et al.Comparing sediment bacterial communities in the macrophyte-dominated and algae-dominated areas of eutrophic Lake Taihu,China[J].Canadian Journal of Microbiology,2011,57(4):263-272.
[29]Huang R,Zhao D Y,Jiang C L,et al.Heterogeneity of bacterial community compositions in surface sediments of three lake zones in Lake Taihu[J].Proceedings of the National Academy of Sciences,India Section B:Biological Sciences,2015,85(2):1-10.
[30]Yang Z,Zhang M,Shi X L,et al.Nutrient reduction magnifies the impact of extreme weather on cyanobacterial bloom formation in large shallow Lake Taihu(China)[J].Water Research,2016,103:302-310.
[31]Paerl H W,Huisman J.Blooms like it hot[J].Science,2008,320(5872):57-58.
[32]O’Neil J M,Davis T W,Burford M A,et al.The rise of harmful cyanobacteria blooms:The potential roles of eutrophication and climate change[J].Harmful Algae,2012,14:313-334.
[33]宜兴日报.太湖蓝藻打捞量是去年同期的5.5倍[EB/OL].http://www.yixing.gov.cn/zxzx/show-13593314.heml2017-05-16/2017-06-26.
[34]陈楠.太湖沉积物微生物群落组成与物质循环及蓝藻爆发的相关性[D].北京:中国农业大学,2015.
[35]Yu D,Yang Y,Zhen W,et al.Spatiotemporal variation of planktonic and sediment bacterial assemblages in two plateau freshwater lakes at different trophic status[J].Applied Microbiology and Biotechnology,2016,100(9):4161-4175.
[36]GB/3838-2002地表水环境质量标准[S].
[37]Winters A D,Marsh T L,Brenden T O,et al.Molecular characterization of bacterial communities associated with sediments in the Laurentian Great Lakes[J].Journal of Great Lakes Research,2014,40(3):640-645.
[38]Jing W,Yi L,Wang P F,et al.Response of bacterial community compositions to different sources of pollutants in sediments of a tributary of Taihu Lake,China[J].Environmental Science and Pollution Research International,2016,23(14):13886-13894.
[39]Liu X,Lu X H,Chen Y W.The effects of temperature and nutrient ratios on Microcystis blooms in Lake Taihu,China:an11-year investigation[J].Harmful Algae,2011,10(3):337-343.
[40]Wang X Y,Sun M J,Wang J M,et al.Microcystis genotype succession and related environmental factors in Lake Taihu during cyanobacterial blooms[J].Microbial Ecology,2012,64(4):986-999.
[41]Otten T G,Xu H,Qin B Q,et al.Spatiotemporal patterns and ecophysiology of toxigenic microcystis blooms in Lake Taihu,China:implications for water quality management[J].Environmental Science and Technology,2016,46(6):3480-3488.
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