氧化还原介体催化强化Shewanella oneidensis MR-1还原亚硒酸盐的研究
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摘要
针对微生物还原亚硒酸盐过程普遍存在的时间较长的问题,考察了4种水溶性醌类介体(α-AQS,AQS,1,5-AQDS和AQDS)对奥奈达希瓦氏菌(Shewanella oneidensis MR-1)还原亚硒酸盐过程的加速作用,通过单因素试验优化了培养条件,对硒纳米颗粒的Zeta电位和粒径进行了表征。结果表明,4种醌类介体都加速了亚硒酸盐的还原,其中,AQDS的加速效果最显著;在pH值为8.0,温度为30℃,AQDS浓度为0.2mmol/L条件下,48h时亚硒酸盐的转化率达到100%;加入AQDS后会生成更大尺寸的硒纳米颗粒,并可能使硒纳米颗粒表面包裹的有机物质成分及含量发生改变。研究结果为Shewanella oneidensis MR-1修复亚硒酸盐污染的实际应用提供了理论依据。
Accelerating the selenite reduction rate is important in selenite bioreduction by microorganism process.This studyinvestigates the impact of redox mediators(α-AQS,AQS,1,5-AQDS and AQDS)on bioreduction of selenite by Shewanella oneidensis MR-1 and optimizes the bioreduction conditions with Shewanella oneidensis MR-1 and AQDS.The selenium particles obtained from cultures are analyzed using Zeta potential analyzer.The results suggest that all four tested redox mediators are able to accelerate the reduction of selenite by Shewanella oneidensis MR-1,and AQDS is the best.The optimum conditons are AQDS concentration of 0.2 mmol/L pH of 8.0 and temperature of 30 ℃.Selenite is completely removed after 48 h bioreduction by Shewanella oneidensis MR-1 with optimum conditons.Bigger size selenium nanoparticles(SeNPS)can be obtained with AQDS.AQDS may influence the composition and content of the organic layer covering around selenium nanoparticles(SeNPs).This study could provide a foundation for particle application in selenite biodegradation.
Accelerating the selenite reduction rate is important in selenite bioreduction by microorganism process.This studyinvestigates the impact of redox mediators(α-AQS,AQS,1,5-AQDS and AQDS)on bioreduction of selenite by Shewanella oneidensis MR-1 and optimizes the bioreduction conditions with Shewanella oneidensis MR-1 and AQDS.The selenium particles obtained from cultures are analyzed using Zeta potential analyzer.The results suggest that all four tested redox mediators are able to accelerate the reduction of selenite by Shewanella oneidensis MR-1,and AQDS is the best.The optimum conditons are AQDS concentration of 0.2 mmol/L pH of 8.0 and temperature of 30 ℃.Selenite is completely removed after 48 h bioreduction by Shewanella oneidensis MR-1 with optimum conditons.Bigger size selenium nanoparticles(SeNPS)can be obtained with AQDS.AQDS may influence the composition and content of the organic layer covering around selenium nanoparticles(SeNPs).This study could provide a foundation for particle application in selenite biodegradation.
引文
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[2]宁婵娟,吴国良.微量元素硒与人体健康及我国富硒食品的开发状况[J].山西农业科学,2009,37(5):88-90.NING Chanjuan,WU Guoliang.Trace element selenium and human health,and development situation of Se-enriched food in China[J].Journal of Shanxi Agricultural Sciences,2009,37(5):88-90.
[3]王玉标.硒与人体健康[J].安徽教育学院学报,2003,21(3):43-44.WANG Yubiao.Selenium and human health[J].Journal of Anhui Institute of Education,2003,21(3):43-44.
[4]朱建明,梁小兵,凌宏文,等.环境中硒存在形式的研究现状[J].矿物岩石地球化学通报,2003,22(1):75-81.ZHU Jianming,LIANG Xiaobing,LING Hongwen,et al.Advances in studying occurrence modes of selenium in environment[J].Bulletin of Mineralogy,Petrology and Geochemistry,2003,22(1):75-81.
[5]LAMPIS S,ZONARO E,BERTOLINI C,et al.Selenite biotransformation and detoxification by Stenotrophomonas maltophilia SeITE02:Novel clues on the route to bacterial biogenesis of selenium nanoparticles[J].Journal of Hazardous Materials,2017,324(Part A):3-14.
[6]黄辉.活性炭吸附法去除水中硒的研究[D].昆明:昆明理工大学,2005.HUANG Hui.Studying on Removal of Selenium from Water by Activated Carbon Process[D].Kunming:Kunming University of Science and Technology,2005.
[7]王琳,施永生,毛云.离子交换法去除水中硒的研究[J].有色金属设计,2005,32(1):53-57.WANG Lin,SHI Yongsheng,MAO Yun.Study on selenium removal from water by ion-exchange process[J].Nonferrous Metals Design,2005,32(1):53-57.
[8]朱建明,雷磊,肖湘,等.地衣芽孢杆菌对亚硒酸盐的还原[J].矿物岩石地球化学通报,2011,30(3):245-250.ZHU Jianming,LEI Lei,XIAO Xiang,et al.The reduction of selenite by Bacillus licheniformis[J].Bulletin of Mineralogy,Petrology and Geochemistry,2011,30(3):245-250.
[9]李丹,李保珍,张龙颜,等.光合细菌还原亚硒酸盐为红色单质硒的条件优化[J].山西农业科学,2015,43(5):543-547.LI Dan,LI Baozhen,ZHANG Longyan,et al.Optimizing experimental conditions for the reduction of selenite to red elemental selenium by photosynthetic bacteria Rhodopseudomonas palustris[J].Journal of Shanxi Agricultural Sciences,2015,43(5):543-547.
[10]蒋华东,何晓红,张礼霞,等.一株假单胞菌(Pseudomonas alcaliphila MBR)好氧还原亚硒酸钠为红色单质硒[J].微生物学报,2010,50(10):1347-1352.JIANG Huadong,HE Xiaohong,ZHANG Lixia,et al.Reduction of selenite to elemental red selenium under aerobic condition by Pseudomonas alcaliphila MBR[J].Acta Microbiologica Sinica,2010,50(10):1347-1352.
[11]MISHRA R R,PRAJAPATI S,DAS J,et al.Reduction of selenite to red elemental selenium by moderately halotolerant Bacillus megaterium strains isolated from Bhitarkanika mangrove soil and characterization of reduced product[J].Chemosphere,2011,84(9):1231-1237.
[12]LI Daobo,CHENG Yuanyuan,WU Chao,et al.Selenite reduction by Shewanella oneidensis MR-1is mediated by fumarate reductase in periplasm[J].Scientific Reports,2014,4(6168):3735.
[13]WADHWANI S A,SHEDBALKAR U U,SINGH R,et al.Biogenic selenium nanoparticles:Current status and future prospects[J].Applied Microbiology and Biotechnology,2016,100(6):2555-2566.
[14]ZHOU Nanqing,TIAN Lijiao,WANG Yucai,et al.Extracellular biosynthesis of copper sulfide nanoparticles by Shewanella oneidensis MR-1as a photothermal agent[J].Enzyme&Microbial Technology,2016,95:230-235.
[15]XIA Zechao,CHENG Yuanyuan,KONG Wanqin,et al.Electron shuttles alter selenite reduction pathway and redistribute formed Se(0)nanoparticles[J].Process Biochemistry,2016,51(3):408-413.
[16]GUO Jianbo,ZHOU Jiti,WANG Dong,et al.Biocalalyst effects of immobilized anthraquinone on the anaerobic reduction of azo dyes by the salt-tolerant bacteria[J].Water Research,2007,41(2):426-432.
[17]王倩,郭延凯,牛博贤,等.生物降解高氯酸盐的影响因素研究[J].河北科技大学学报,2015,36(6):652-658.WANG Qian,GUO Yankai,NIU Boxian,et al.Study on the affecting factors of perchlorate biodegradation[J].Journal of Hebei University of Science and Technology,2015,36(6):652-658.
[18]陈延明,张华雨,赵丽君,等.氧化还原介体在环境治理中应用研究进展[J].河北工业科技,2013,30(4):266-271.CHEN Yanming,ZHANG Huayu,ZHAO Lijun,et al.Development and application of redox mediators for environmental protection[J].Hebei Journal of Industrial Science and Technology,2013,30(4):266-271.
[19]DUNNIVANT F M,SCHWARZENBACH R P,MACALADY D L.Reduction of substituted nitrobenzenes in aqueous solutions containing natural organic matter[J].Environmental Science&Technology,1992,26(11):2133-2141.
[20]GU Baohua,CHEN Jie.Enhanced microbial reduction of Cr(Ⅵ)and U(Ⅵ)by different natural organic matter fractions[J].Geochimica et Cosmochimica Acta,2003,67(19):3575-3582.
[21]WANG Xiujuan,LIU Guangfei,ZHOU Jiti,et al.Quinone-mediated reduction of selenite and tellurite by Escherichia coli[J].Bioresource Technology,2011,102(3):3268-3271.
[22]ZHANG Y,ZAHIR Z A,AMRHEIN C,et al.Application of redox mediator to accelerate selenate reduction to elemental selenium by Enterobacter taylorae[J].Journal of Agricultural and Food Chemistry,2007,55(14):5714-5717.
[23]HE Qiang,YAO Kai.Impact of alternative electron acceptors on selenium(Ⅳ)reduction by Anaeromyxobacter dehalogenans[J].Bioresource Technology,2011,102(3):3578-3580.
[24]PEARCE C I,PATTRICK R A,LAW N,et al.Investigating different mechanisms for biogenic selenite transformations:Geobacter sulfurreducens,Shewanella oneidensis and Veillonella atypica[J].Environmental Technology,2009,30(12):1313-1326.
[25]郭延凯,马志远,郭建博,等.醌介体催化强化酸性红B生物脱色[J].环境工程学报,2013,7(5):1739-1743.GUO Yankai,MA Zhiyuan,GUO Jianbo,et al.Enhanced biodecolorization of Acid Red B catalyzed by quinone mediators[J].Chinese Journal of Environmental Engineering,2013,7(5):1739-1743.
[26]李海波,廉静,郭延凯,等.氧化还原介体催化强化Paracoccus versutus菌株GW1反硝化特性研究[J].环境科学,2012,33(7):2458-2463.LI Haibo,LIAN Jing,GUO Yankai,et al.Biocatalyst of redox mediators on the denitrification by Paracoccus versutus strain GW1[J].Environmental Science,2012,33(7):2458-2463.
[27]黄丹,王竞,李培良,等.Shewanella sp.XB缺氧反硝化降解苯酚[J].环境工程学报,2014,8(4):1345-1349.HUANG Dan,WANG Jing,LI Peiliang,et al.Nitrate-dependent phenol degradation by Shewanella sp.XB under anoxic conditions[J].Chinese Journal of Environmental Engineering,2014,8(4):1345-1349.
[28]周群英,王士芬.环境工程微生物学[M].3版.北京:高等教育出版社,2008.
[29]刘金香.奥奈达希瓦氏菌铀(Ⅵ)还原能力的增强机制及应用基础研究[D].衡阳:南华大学,2015.LIU Jinxiang.Study on Mechanism and Fundamental Application of U(Ⅵ)Reduction by Shewanella oneidensis[D].Hengyang:University of South China,2015.
[30]KHOEI N S,LAMPIS S,ZONARO E,et al.Insights into selenite reduction and biogenesis of elemental selenium nanoparticles by two environmental isolates of Burkholderia fungorum[J].New Biotechnology,2017,34:1-11.
[31]JAIN R,JORDAN N,WEISS S,et al.Extracellular polymeric substances govern the surface charge of biogenic elemental selenium nanoparticles[J].Environmental Science&Technology,2015,49(3):1713-1720.
[32]LENZ M,KOLVENBACH B,GYGAX B,et al.Shedding light on selenium biomineralization:Proteins associated with bionanominerals[J].Applied and Environmental Microbiology,2011,77(13):4676-4680.
[33]LAMPIS S,ZONARO E,BERTOLINI C,et al.Delayed formation of zero-valent selenium nanoparticles by Bacillus mycoides SeITE01as a consequence of selenite reduction under aerobic conditions[J].Microbial Cell Factories,2014,13(1):35-48.
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